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Method for detecting mercury ions through gold electrode modified by polythymidine DNA

A multi-thymine and thymine technology, applied in the direction of material electrochemical variables, measuring devices, scientific instruments, etc., can solve the problems of large detection instruments and inapplicability, and achieve the effect of low background signal, simple instrument and good selectivity

Inactive Publication Date: 2012-03-14
EAST CHINA NORMAL UNIV
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Problems solved by technology

Since both fluorescence detection and ultraviolet detection require relatively large and expensive detection instruments, these methods are not suitable for some relatively simple workplaces and field operations.

Method used

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  • Method for detecting mercury ions through gold electrode modified by polythymidine DNA
  • Method for detecting mercury ions through gold electrode modified by polythymidine DNA

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Embodiment 1

[0020] (1) Preparation of polythymine DNA-modified gold electrodes

[0021] The electrochemically treated fresh and clean gold electrode was placed in 1 μM sulfhydryl-modified polythymine oligonucleotide (PTO) solution, self-assembled at room temperature for 16 h, the gold electrode was taken out, and washed with 10 mM HEPES solution (pH 7.2). Unassembled PTO was removed, then placed in 1 mM mercaptohexanol solution at room temperature for 2 h, and washed with the same HEPES solution (pH 7.2) to remove unassembled mercaptohexanol. To prevent solution evaporation, the electrodes remain sealed throughout the assembly process. The prepared electrodes are PTO / Au electrodes.

[0022] (2) Detection of mercury ions in river water samples

[0023] Soak the prepared PTO / Au electrode in a series of 10mM HEPES solution (pH 7.2) of standard water samples containing different concentrations of mercury ions for 15min, then wash twice with 10mM HEPES solution (pH 7.2); wash with 10mM HEPES...

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Abstract

The invention provides a method for detecting mercury ions through a gold electrode modified by polythymidine DNA, which is characterized in that polythymidine DNA molecule is taken as a recognition element for mercury ions, the gold electrode modified by polythymidine DNA is prepared, then mercury ions in an aqueous solution is detected by the prepared gold electrode, wherein alkali base T in DNA molecule is capable of specifically binding with mercury ions to form a stable T-Hg<2+>-T structure. The intensity of an electrochemical signal is relative with the concentration of mercury ions in the solution, so that the concentration of mercury ions in the aqueous solution can be calculated. According to the invention, the method for detecting mercury ions has the advantages of no pollution on environment, good selectivity on mercury ions, eliminated interference of most metal ions, high sensitivity, simple required apparatus and low cost, and is suitable for being applicable to such humble situations as fields and the like.

Description

technical field [0001] The invention relates to a method for detecting and analyzing the concentration of mercury ions, in particular to a method for directly electrochemically detecting mercury ions in aqueous solution by using a polythymine DNA-modified gold electrode. Background technique [0002] Since the structure and properties of DNA are very sensitive to the influence of heavy metals, it is of great value and significance to develop highly selective and sensitive heavy metal analysis and detection methods using the interaction between DNA and metal ions. Gao et al. proved that guanine in DNA can be combined with Cu 2+ Specific binding forms a stable structure, (Y.G.Gao, M.Sriam, A.H.J.Wang, Nucleic Acid Res.21(1993)4093-4191) and used for Cu 2+ testing. The Ono group reported for the first time that the base pair C-C in DNA can interact with Ag + Specific binding to form stable C-Ag + -C structure (Miyake, Y.; Togashi, H.; Tashiro, M.; Yamaguchi, H.; Oda, S.; Ku...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/333
Inventor 何品刚吴继魁王霞崔静徐颖程圭芳
Owner EAST CHINA NORMAL UNIV
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