A method for detecting bio-available
cadmium in soil includes steps: S100,
sample collection and pretreatment, to be more specific, sampling soil, subjecting sampled soil to
air drying at the
room temperature,
grinding, and filtering through a 200-mesh screen to obtain a soil sample; weighing 1-5g of the soil sample, adding into a 250mL
polytetrafluoroethylene centrifugal cup, adding 25mL of 1mol / L
ammonium chloride solution and 25mL of 80%
ethanol solution, cleaning through an ultrasonic cleaner, centrifuging through a
centrifuge, filtering supernatant through a filter membrane to obtain extract liquid, and adopting HNO3 solution in concentration of 1% to obtain a constant volume; S200, determination of the concentration of bio-available
cadmium in the extract liquid, to be more specific,adopting an
inductively coupled plasma source
mass spectrometer for creating a
standard curve for determining different concentrations of bio-available
cadmium according to an
inductively coupled plasma mass spectrometry method; adopting the
inductively coupled plasma source
mass spectrometer for measuring concentration of bio-available cadmium in the extract liquid according to the
standard curve; S300, result analysis, to be more specific, calculating the content of bio-available cadmium in the soil sample according to measured soil
sample mass and measured extract liquid concentration.