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Preparation method of sea cucumber active polypeptide under ultrasonic extraction

A technology of active peptides and ultrasound, applied in the preparation method of peptides, chemical instruments and methods, medical preparations containing active ingredients, etc., can solve the problems of reduced effect and loss of active substances in sea cucumbers, and achieve simple instruments and high automation , the effect of convenient operation

Inactive Publication Date: 2010-03-24
大连正大海洋生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the traditional processing method, the active substances of sea cucumbers are lost and their effects are also reduced. After research, the quality of active ingredients in sea cucumbers is relatively complete. How to retain the maximum limit in processing has always been the goal pursued by scientists and enterprises.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take 100kg of fresh and alive sea cucumbers, remove the viscera and the sediment in the sea cucumber intestines, put them into a 300L stainless steel cleaning tank, add edible water to rinse three times, and remove them after cleaning. Calculate 60kg according to the peeling rate of 60%. Sea cucumber body. Start the stainless steel meat grinder, and grind the sea cucumber and sea cucumber sausage into fine pieces. Get sea cucumber minced 60kg and put into 100kg stainless steel drums respectively, with an average of 15kg per barrel, add deionized water 5 times the weight of sea cucumber minced sea cucumbers in four stainless steel drums, add 75kg per barrel on average. Start the TM-2 type colloid mill, set 160 mesh for the first time, set 290 mesh for the second time, grind twice repeatedly, get sea cucumber slurry 360kg.

[0025] Start the thick slurry pump to input 360kg of sea cucumber slurry into the vacuum external 600L ultrasonic reactor, close the tank mouth, adj...

Embodiment 2

[0029] According to embodiment 1 process, differ from embodiment 1 in that:

[0030] (1) Take 100 kg of fresh and live white sea cucumber, remove the viscera to obtain 70 kg of white sea cucumber material, mince it, add water, and make 210 kg of slurry.

[0031] (2) Ultrasonic extraction power is 500W, transmission frequency is 15KHz, power density of transmission end face is 50W / cm 2 , the pulse cycle frequency is 8 times per second, and the sound velocity is 1000 m / s, so that the white sea cucumber slurry generates vortex in the reaction kettle, and ultrasonic extraction is performed three times under the conditions of vacuuming 80Pa and temperature 26°C, each time for 20min, and the interval time is 40min. Then filter with a 100-200 mesh duplex filter to obtain 210 kg of ultrasonically extracted white japonicus active polypeptide liquid.

[0032] (3) Adopt the high-speed butterfly centrifuge of 6000r / min to separate for 10min, obtain 200kg of separation liquid, add 2 time...

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Abstract

The invention relates to a deep processing method of sea cucumber, concretely a preparation method of sea cucumber active polypeptide under ultrasonic extraction. The preparation method comprises thefollowing steps: A preparing fresh paste of sea cucumber; B extracting sea cucumber polypeptide using ultrasonic wave; C purifying sea cucumber polypeptide using ultra-micro film; D performing concentration at low temperature; E centrifugally spray-drying. The positive effects of the invention are that the sea cucumber polypeptide is extracted using the ultrasonic wave technology to increase the deep processing rate of the sea cucumber, effectively keep the active component in the sea cucumber, thus the collagen of the sea cucumber is not invariant and the helical state of the collagen is keptand the activity is added and the active polypeptide of the sea cucumber is uniformly distributed under purification of ultra-micro film; the extraction process does not need high temperature, thereby preventing decomposing the polypeptide, polysaccharide, saponin molecular structure of the sea cucumber due to high temperature and conquering the defects of the conventional extraction that the polypeptide, polysaccharide molecular structure is broken and the activity is reduced; the instrument is simple, the operation is convenient and the autopadicity of the ultrasonoscope is very high and the preparation method is convenient for industrialization. The sea cucumber active polypeptide can be prepared into capsules or tablets capable of invigorating the kidney and supporting yang, benefiting vital energy and invigorating yin, producing and enriching the blood, moistening the intestines and removing the dryness and depressing tumor diffusion.

Description

technical field [0001] The invention relates to deep processing of sea cucumbers, in particular to a preparation method for ultrasonically extracting sea cucumber active polypeptides. Background technique [0002] "Sea cucumber is warming and nourishing, so it is the enemy's ginseng." Sea cucumbers are rich in nutrients, especially collagen protein, which dissolves well in water and is easily digested and absorbed by the human body. Sea cucumber has a strong effect of tonifying kidney and strengthening yang, nourishing qi and nourishing yin, promoting blood and nourishing blood, moistening intestines and removing dryness, and inhibiting the spread of tumors. Due to the traditional processing method, the active substances of sea cucumbers are lost and their effects are also reduced. After research, the quality of active ingredients in sea cucumbers is relatively complete. How to preserve the maximum limit during processing has always been the goal pursued by scientists and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/34C07K1/14C07K2/00A61K38/02A61K9/20A61K9/48A61P13/12A61P35/00
Inventor 李长青张世平陈静平李晓龙
Owner 大连正大海洋生物有限公司
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