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Preparation method of Euphorbia factor L3

A technology of Euphorbia factor and euphorbia seed, applied in the field of preparation of Euphorbia factor L3, can solve the problems of hindering the progress of EuphorbiaFactorL3, difficult to achieve extraction of EuphorbiaFactorL3, etc., and achieves the effects of stable process, simple operation and high purity

Inactive Publication Date: 2015-12-16
TIANJIN YAOYU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But so far, the method of extracting Euphorbia Factor L3 (EuphorbiaFactorL3) by liquid chromatography in natural product extracts has not been reported, and it is even more difficult to extract high-purity or monomer EuphorbiaFactorL3, which is also to a certain extent Blocking progress of EuphorbiaFactorL3 in development as a traditional Chinese medicine

Method used

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  • Preparation method of Euphorbia factor L3
  • Preparation method of Euphorbia factor L3
  • Preparation method of Euphorbia factor L3

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Obtaining the primary extract of QJZ

[0035] 1. Crushing:

[0036] Take Qianjinzi as a raw material, put it into a pulverizer for pulverization.

[0037] 2. Organic reagent extraction:

[0038] The stephenia powder was extracted with organic reagents, three consecutive extractions, each extraction time was 6h (heated to 60 degrees, and then heated for 6h). specific:

[0039] The first organic reagent extraction: add 2.5 kg of Stephania chinensis powder to 20 L of organic reagent, the organic reagent is ethyl acetate, stir well until the material liquid is uniform and there are no obvious lumps, turn on the water bath, extract for 6 hours, after the extraction is completed, collect the The supernatant was filtered with filter paper, and the supernatant was concentrated in a rotary evaporator and then transferred to a constant temperature blast drying oven at 60°C for drying to obtain the initial QJZ extract, which was precipitated for use;

[0040] The second org...

Embodiment 2

[0047] 1. Obtaining the primary extract of QJZ

[0048] 1. Crushing:

[0049] Take Qianjinzi as a raw material, put it into a pulverizer for pulverization.

[0050] 2. Organic reagent extraction:

[0051] The stephenia powder was extracted with organic reagents, three consecutive extractions, each extraction time was 6h (heated to 60 degrees, and then heated for 6h). specific:

[0052] The first organic reagent extraction: add 2.5 kg of Stephania chinensis powder to 20 L of organic reagent, the organic reagent is ethyl acetate, stir well until the material liquid is uniform and there are no obvious lumps, turn on the water bath, extract for 6 hours, after the extraction is completed, collect the The supernatant was filtered with filter paper, and the supernatant was concentrated in a rotary evaporator and then transferred to a constant temperature blast drying oven at 60°C for drying to obtain the initial QJZ extract, which was precipitated for use;

[0053] The second org...

Embodiment 3

[0060] 1. Obtaining the primary extract of QJZ

[0061] 1. Crushing:

[0062] Take Qianjinzi as a raw material, put it into a pulverizer for pulverization.

[0063] 2. Organic reagent extraction:

[0064] The stephenia powder was extracted with organic reagents, three consecutive extractions, each extraction time was 6h (heated to 60 degrees, and then heated for 6h). specific:

[0065] The first organic reagent extraction: add 2.5 kg of Stephania chinensis powder to 20 L of organic reagent, the organic reagent is ethyl acetate, stir well until the material liquid is uniform and there are no obvious lumps, turn on the water bath, extract for 6 hours, after the extraction is completed, collect the The supernatant was filtered with filter paper, and the supernatant was concentrated in a rotary evaporator and then transferred to a constant temperature blast drying oven at 60°C for drying to obtain the initial QJZ extract, which was precipitated for use;

[0066] The second org...

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Abstract

The invention relates to a preparation method of a Euphorbia factor L3. The Euphorbia Factor L3 is extracted from leptochloa chinensis by virtue of a two-dimensional liquid chromatographic technique; by virtue of the reasonable selection of a chromatographic column, a dissolved solution, a mobile phase and the like and the optimization of technical conditions such as the flow velocity, the elution mode and the collection section and the complementary separation through the orthogonality of the first dimension and the second dimension, the whole separation and purification method is practicable, the operability is high, and the purity of the obtained Euphorbia Factor L3 can reach up to 97% or more; the process of the whole process is stable, the operation is simple and convenient, and the separation efficiency is high.

Description

technical field [0001] The invention relates to the field of compound production, in particular to a preparation method of euphorbia factor L3. Background technique [0002] Qianjinzi is the dry mature seed of Euphorbialathyris, which is one of the traditional Chinese medicinal materials in my country. Fructus Qianjinzi is warm in nature, pungent in taste, and slightly poisonous. Its main functions are to expel water to reduce swelling and eliminate blood stasis. , warts. In foreign countries, the plant seeds have long been used for cancer treatment records, and its methanol extract has been shown to have anti-cancer effects in preliminary screening assays for anti-malignant tumor-related activities. EuphorbiaFactorL3 is such a class of compounds, which is an important natural active ingredient widely present in Euphorbiaceae plants, and its structure is as follows: [0003] [0004] Euphorbia Factor L3R 1 = benzoy [0005] More and more studies have shown that polyc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/78C07C67/48C07C67/56A61P35/00
CPCC07C67/48C07C67/56C07C2602/32C07D213/80C07D213/803C07C69/78
Inventor 张耀洲杨珊珊杜思邈盖其静马红庞莉王欢欢陆洪韩朝李上文
Owner TIANJIN YAOYU BIOLOGICAL TECH
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