Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for separating and purifying follicular cells

A technology for separation and purification of theca, which can be used in cell dissociation methods, biochemical equipment and methods, animal cells, etc., and can solve the problems of slow progress in the research of human theca cells

Active Publication Date: 2022-06-28
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
View PDF10 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This trend has slowed the progress of related research on human theca cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for separating and purifying follicular cells
  • Method for separating and purifying follicular cells
  • Method for separating and purifying follicular cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Separation of ovarian tissue, preliminary filtration to remove granulosa cells

[0033] The discarded follicular fluid was collected in a sterile container on the day of egg retrieval, and allowed to stand for more than 30 minutes.

[0034] The sediment at the bottom of the follicular fluid was sucked, placed in a 50 mL sterile centrifuge tube, centrifuged at 500 g for 5 min, the supernatant was discarded, an appropriate amount of PBS was added to the centrifuge tube, and a suspension was formed by pipetting.

[0035] Place the sterilized 100μm cell sieve in a 6cm petri dish, pipette the suspension into the cell sieve in stages, and finally suck the PBS solution to repeatedly wash the ovarian tissue in the cell sieve, in order to remove granulosa cells and granulosa cell clusters by preliminary filtration. piece.

[0036] (2) Isolation of ovarian cells

[0037] Use ophthalmic tweezers to transfer the ovarian tissue on the cell sieve to a 15 mL sterile centrifuge t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for separating and purifying follicular cells, and belongs to the field of cell culture. The method comprises the following steps: collecting sterile follicular fluid discarded in an ovum collection day, and separating to obtain ovarian tissues; digesting the obtained ovarian tissue by using a PBS (Phosphate Buffer Solution) containing type I collagenase, collecting suspension cells, removing undigested follicle membrane tissues in the suspension cells by using a cell sieve, and collecting the ovarian cells; after the ovarian cells are resuspended, low-speed short-time centrifugation is carried out, precipitates are collected to obtain primarily purified follicular cells, then the primarily purified follicular cells are laid and planted in an uncoated culture dish, the follicular cells are further purified, and the separated and purified follicular cells are obtained. The method can overcome the defects of extracting the follicular cells from conventional ovarian tissues, is simple and easy to operate, and can quickly purify the follicular cells, so that a new idea and a technical route are provided for the source of the follicular cells, and a new breakthrough is brought to the research bottleneck of the follicular cells and the ovarian tissues.

Description

technical field [0001] The invention relates to the field of cell culture, in particular to a method for separating and purifying theca cells. Background technique [0002] Polycystic ovary syndrome (PCOS) is one of the most common endocrine diseases in women of reproductive age, with an incidence of 5-10%. The main clinical manifestations are oligomenorrhea or amenorrhea, abnormal uterine bleeding, infertility, and hirsutism. , acne. PCOS patients are at increased risk of developing gestational diabetes mellitus and gestational hypertension during pregnancy. Long-term complications of PCOS include insulin resistance, impaired glucose tolerance, type 2 diabetes, obesity, dyslipidemia, heart disease, hypertension, depression, sleep apnea syndrome, and endometrial cancer. PCOS endangers women's health for a long time. However, due to the unclear etiology and pathogenesis, there is no cure. Patients need lifelong follow-up and lifelong treatment. Increased androgen plays a k...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0682C12N2509/00
Inventor 陈明晖郭希钟依平刘洋
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com