36 results about "Ovary cell" patented technology

The invention aims at providing an establishment method of an ovary cell line of cynoglossus semilaevis, which comprises the following steps: 1) removing the outermost membrane of the ovary tissue of cynoglossus semilaevis under a sterile condition, cutting the tissue into small blocks in a culture solution, flushing the tissue blocks with a PBS solution and digesting with a trypsin solution, wherein the solution after trypsin digestion contains cells, cell clusters and tissue blocks not completely digested; and 2) centrifuging the solution to remove the supernatant trypsin, and suspending the cells, cell clusters and small tissue block precipitates with a culture solution; after a suspension is obtained, inoculating into a culture plate treated by the type-I collagen; adding a culture solution, and culturing in a biochemical incubator at 24 DEG C; and performing subculture to finish establishment of the ovary cell line. In the establishment method provided by the invention, an ovary cell line of cynoglossus semilaevis is successfully established by use of the ovary tissue of cynoglossus semilaevis, the established cell line can be continuously passed, the passage cells obtained by the method can be passed for over 60 generations, and a great quantity of ovary cells can be provided.

The invention provides a transgenic insect cell line for high-yield baculovirus, and a preparation method and the application thereof. The name of the transgenic insect cell line is IOZCAS-Spex IX and the preservation number of the cell line is CGMCC No.4506. The preparation method of the transgenic insect cell line comprises the steps as follows: spodoptera exigua ovary cells are cultured; humantelomerase reverse transcriptase genes are transformed into known expression vectors to obtain recombinant vectors; the recombinant vectors are introduced into the spodoptera exigua ovary cells; and the spodoptera exigua ovary cells are enabled to express human telomerase reverse transcriptase, so as to obtain the transgenic insect cell line. The transgenic insect cell line is applied to the production of the baculovirus.

The invention discloses an establishment method for a pomacea canaliculata ovary cell line and belongs to the technical field of freshwater organism cell culture. The establishment method includes the following steps that cell culture fluid is prepared; female pomacea canaliculata with the body weight of 30-40 g is selected to be raised in sterile water; the surface of the female pomacea canaliculata is disinfected; the pomacea canaliculata is dissected to obtain ovarian tissue; the ovarian tissue is cleaned with PBS cleaning fluid and cut into pieces; a cell culture bottle is inoculated with the treated ovarian tissue; the culture bottle is placed in a 5% CO2 culture box, and constant temperature culture is conducted at the temperature of 28 DEG C; after overnight treatment, a proper amount of cell culture fluid is added, and the tissue is immersed; the cell culture fluid is sucked out and replaced with an equal amount of cell culture fluid every other 5 days till the culture bottle is filled with extended and proliferated cells. The pomacea canaliculata ovary tissue cell line can be rapidly and repeatedly established within a short time, needed cell culture equipment is simple, and operability is high. The establishment method is an important supplement for culture of cells of aquatic invertebrates and provides fundamental data for research on the immunologic mechanism of the aquatic invertebrates.

The invention relates to a health food formula applicable for ovary maintenance, endocrine modulation, anti-oxidation and senility delay of female and a method for preparing the health food formula, belonging to the technical field of health food. The health food is refined by adopting soybean isoflavone with natural plant functionality and good moistening function for human body as a main raw material, and porphyrin iron, isomaltose hypgather, biological calcium carbonate, raspberry, vitamin and the like as auxiliary materials. According to the health food in the technology formula, the good effects of the soybean isoflavone on free radical removing and ovary endocrine balance modulation are expressed, the isomaltose hypgather can facilitate the absorption of the soybean isoflavone in the human body, the biological utilization rate of the soybean isoflavone is increased, and thus the intake amount of the soybean isoflavone can be reduced; and simultaneously, the technology formula is also added with the nutrient elements including iron, calcium, vitamin and the like for facilitating the metabolic balance of blood and bone calcium in the human body, thus a senescent process of ovary cells of the female can be slowed down, and the health food formula preparation without side effect is provided for wide female friends.

The present invention discloses composing components of an antheraea pernyi ovary cell culture medium, a preparation method of the antheraea pernyi ovary cell culture medium, and an application of the antheraea pernyi ovary cell culture medium in antheraea pernyi ovary cell culture. According to the present invention, a prepared amino acid and sugar mixing storage solution (MLM-AAS), an inorganicsalt storage solution (MLM-Salt), a vitamin storage solution (MLM-Vit), bovine serum albumin, fetuin and the like are prepared according to a certain ratio, and the pH value is adjusted to 6.2-6.4 toobtain a MLM-45 culture medium, wherein the prepared MLM-45 culture medium is added with fetal bovine serum and a penicillin and streptomycin mixing solution according to a certain volume ratio before the prepared MLM-45 culture medium is used, the volume ratio of the fetal bovine serum is 20%, and the volume ratio of the penicillin and streptomycin mixing solution is 1%. With a plurality of experiments, the following in vitro cell growth conditions are determined that: the culture temperature is 26-28 DEG C, the pH value is 6.2-6.4, and the osmotic pressure is 315-350 mOsm / kg. With the present invention, technical services are provided for researches in antheraea pernyi biology, antheraea pernyi pathogenic microorganism and related fields.

The invention provides a high-yield baculovirus ovary cells line induced by a carcinogen, a preparation method and an application, a name of the cell line is IOZCAS-Spex12, and a preservation number is CGMCC No. 4804, the preparation method comprises the following steps: culturing beet armyworm ovary cells; and using the carcinogen to induce and converse the beet armyworm ovary cells obtained in the step 1 to obtain the high-yield baculovirus ovary cells line induced and conversed by the carcinogen; and the invention also relates to the application of high-yield baculovirus ovary cells line in baculovirus production.

The invention relates to the field of animals, in particular to a method for detecting a decapterus maruadsi oocyte development time phase. According to the invention, development of oocyte in ovary is researched by adopting a biological tissue section technology. The result shows that a nucleo-cytoplasmic ratio of the oocyte in the ovary is between 0.03 and 0.3, and is obviously reduced for three times totally along with the oocyte development. According to the nucleo-cytoplasmic ratio change of the oocyte, the oocyte development is divided into five stages by virtue of cell volume, nucleus development, and the development conditions of yolk nucleus, follicle cells and the like. Analysis of the area ratio of the oocyte in the ovary at each stage shows that oocytes with different time phases exist in the ovary, so the decapterus maruadsi has the asynchronous spawning characteristic. Compared with the traditional visual-inspection staging method, the histological technique adopted by the research is capable of easily distinguishing the gender, and the staging result is accurate.

The invention provides a serum-free medium for CHO cell culture. Said culture medium is based on DMEM / F12 with addition of transferrin, insulin, ethanolamine and other substances. The serum-free medium of the invention can make CHO cells grow vigorously, and the cell density and cell activity are comparable to those of the serum-containing medium, and can completely replace the serum-containing medium for the cultivation of CHO cells.

The invention provides a transgenic insect cell line for high-yield baculovirus, and a preparation method and the application thereof. The name of the transgenic insect cell line is IOZCAS-Spex IX and the preservation number of the cell line is CGMCC No.4506. The preparation method of the transgenic insect cell line comprises the steps as follows: spodoptera exigua ovary cells are cultured; human telomerase reverse transcriptase genes are transformed into known expression vectors to obtain recombinant vectors; the recombinant vectors are introduced into the spodoptera exigua ovary cells; and the spodoptera exigua ovary cells are enabled to express human telomerase reverse transcriptase, so as to obtain the transgenic insect cell line. The transgenic insect cell line is applied to the production of the baculovirus.

The present invention provides a high-yielding baculovirus pupae ovary cell line of Oriental Armyworm. The name of the cell line is IOZCAS-Myse-1, and its preservation number is CGMCC No.17282. The present invention provides a method for constructing the O. orientalis pupal ovary cell line of the high-yielding baculovirus. The method comprises the following steps: step 1) obtaining the ovary tissue of the oriental armyworm; step 2) cultivating the ovary tissue of the oriental armyworm, until The proliferated cells are filled with the culture flask, wherein the tissue is closely attached to the bottom of the cell culture flask; step 3) take the newly proliferated single cell and continue to culture until the cell is passed down; the whole process is carried out under sterile conditions . The oriental armyworm pupal ovary cell line with high yield of baculovirus provided by the present invention can be used to replicate baculovirus for large-scale production of baculovirus or baculovirus insecticide; the cell line can also be applied to construct Baculovirus expression vector system for expressing proteins of commercial or scientific value.

The present invention relates to newly identified nucleic acids and polypeptides present in normal and neoplastic ovary cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions comprising the nucleic acids, polypeptides, antibodies, variants, derivatives, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating ovarian cancer and non-cancerous disease states in ovary tissue, identifying ovary tissue, monitoring and identifying and / or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered ovary tissue for treatment and research.