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36 results about "Ovary cell" patented technology
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Cell preparation for repairing ovarian functions
InactiveCN105663168APrevent pluripotencyLong-term maintenance of stem cell propertiesPeptide/protein ingredientsMammal material medical ingredientsOvarian functionAdditive ingredient
The invention relates to a cell preparation for restoring ovarian function, including amniotic mesenchymal stem cells and ovarian cells. By using amniotic mesenchymal stem cells combined with ovarian cells to make cell preparations, as well as auxiliary components for preventing the multidirectional differentiation of amniotic mesenchymal stem cells and promoting the activity and normal growth and proliferation of amniotic mesenchymal stem cells and ovarian cells, at the same time, Combined with ovarian cells, amniotic mesenchymal stem cells can maintain their stem cell characteristics for a long time, and then act on the ovary through the cytokines secreted by them combined with ovarian cells to achieve the purpose of immediate and complete restoration of ovarian function; avoid cells made of single cells In the preparation, the cell properties are unstable, resulting in unstable ovarian function repair effect, poor repair effect, and failure to completely repair ovary and other defects.
Owner:SHEN ZHEN ISTEM REGENERATIVE MEDICINE SCI TECH CO LTD
Establishment and application method of ovary cell line of cynoglossus semilaevis
The invention aims at providing an establishment method of an ovary cell line of cynoglossus semilaevis, which comprises the following steps: 1) removing the outermost membrane of the ovary tissue of cynoglossus semilaevis under a sterile condition, cutting the tissue into small blocks in a culture solution, flushing the tissue blocks with a PBS solution and digesting with a trypsin solution, wherein the solution after trypsin digestion contains cells, cell clusters and tissue blocks not completely digested; and 2) centrifuging the solution to remove the supernatant trypsin, and suspending the cells, cell clusters and small tissue block precipitates with a culture solution; after a suspension is obtained, inoculating into a culture plate treated by the type-I collagen; adding a culture solution, and culturing in a biochemical incubator at 24 DEG C; and performing subculture to finish establishment of the ovary cell line. In the establishment method provided by the invention, an ovary cell line of cynoglossus semilaevis is successfully established by use of the ovary tissue of cynoglossus semilaevis, the established cell line can be continuously passed, the passage cells obtained by the method can be passed for over 60 generations, and a great quantity of ovary cells can be provided.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
Saliva test for early diagnosis of cancers
InactiveUS20050106642A1Reduce concentrationReduce amountDisease diagnosisFermentationCancer antigenOvary cell
Proteonic cancer markers (PCMs) for breast, colon, liver and ovary were isolated, from the respective lysate of transformed cells, by differential centrifugation. Polyclonal antibodies were generated in mice against the (PCMs) for breast, colon, liver and ovary individually and combination thereof. Saliva from normal people was assayed by ELISA for antimixture of PCMs; breast, colon, liver and ovary cells individually. It was revealed that cancer antigen was detectable in saliva from normal people and the ELISA titer / 100 μl ranged from 1:200 to 1:1600. Out of 32 normal salivas tested, ELISA titer was higher than 1:1000 in seven specimens. Those specimens were assayed by ELISA tests for individual PCM using anti-breast, anti-colon, anti-liver and anti-ovary. Each saliva specimen showed highest titer for one type of cancer antigen. Four saliva specimens showed high titers for breast PCM, two for colon one for liver. Only one saliva specimen showed high titer for ovary and colon PCMs. Thus, the invention further relates to the quantitative assessment of specific PCMs for breast, colon, liver and ovary in human saliva, by using antibodies against these markers individually.
Owner:LIPPS BINIE V +1
Transgenic insect cell line for high-yield baculovirus, and preparation method and application thereof
InactiveCN102807969AMicroorganism based processesViruses/bacteriophagesGenetically modified insectOvary cell
The invention provides a transgenic insect cell line for high-yield baculovirus, and a preparation method and the application thereof. The name of the transgenic insect cell line is IOZCAS-Spex IX and the preservation number of the cell line is CGMCC No.4506. The preparation method of the transgenic insect cell line comprises the steps as follows: spodoptera exigua ovary cells are cultured; humantelomerase reverse transcriptase genes are transformed into known expression vectors to obtain recombinant vectors; the recombinant vectors are introduced into the spodoptera exigua ovary cells; and the spodoptera exigua ovary cells are enabled to express human telomerase reverse transcriptase, so as to obtain the transgenic insect cell line. The transgenic insect cell line is applied to the production of the baculovirus.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
High-yield baculovirus cell line induced by carcinogen, preparation method and application
The invention provides a high-yield baculovirus ovary cells line induced by a carcinogen, a preparation method and an application, a name of the cell line is IOZCAS-Spex12, and a preservation number is CGMCC No. 4804, the preparation method comprises the following steps: culturing beet armyworm ovary cells; and using the carcinogen to induce and converse the beet armyworm ovary cells obtained in the step 1 to obtain the high-yield baculovirus ovary cells line induced and conversed by the carcinogen; and the invention also relates to the application of high-yield baculovirus ovary cells line in baculovirus production.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
Gene engineering cell line receiving site-directed integration of exogenous genes
InactiveCN108441478ANucleic acid vectorVector-based foreign material introductionOvary cellGene engineering
The present invention relates to an engineering cell line for site-directed integration of exogenous genes, and uses thereof, wherein an identification tag is inserted into the Z4q3 region of Chinesehamster ovary cells (CHO) as host cells to obtain the cell line. According to the present invention, the engineering cell line supports the site-directed integration of exogenous genes, and can maintain the efficient and stable expression of exogenous genes for a long time.
Owner:成都金洛克锶生物技术有限公司
Establishment method for pomacea canaliculata ovary cell line
InactiveCN105861417AContribute to researchImprove training effectInvertebrate cellsFresh water organismOvary cell
The invention discloses an establishment method for a pomacea canaliculata ovary cell line and belongs to the technical field of freshwater organism cell culture. The establishment method includes the following steps that cell culture fluid is prepared; female pomacea canaliculata with the body weight of 30-40 g is selected to be raised in sterile water; the surface of the female pomacea canaliculata is disinfected; the pomacea canaliculata is dissected to obtain ovarian tissue; the ovarian tissue is cleaned with PBS cleaning fluid and cut into pieces; a cell culture bottle is inoculated with the treated ovarian tissue; the culture bottle is placed in a 5% CO2 culture box, and constant temperature culture is conducted at the temperature of 28 DEG C; after overnight treatment, a proper amount of cell culture fluid is added, and the tissue is immersed; the cell culture fluid is sucked out and replaced with an equal amount of cell culture fluid every other 5 days till the culture bottle is filled with extended and proliferated cells. The pomacea canaliculata ovary tissue cell line can be rapidly and repeatedly established within a short time, needed cell culture equipment is simple, and operability is high. The establishment method is an important supplement for culture of cells of aquatic invertebrates and provides fundamental data for research on the immunologic mechanism of the aquatic invertebrates.
Owner:CHINA JILIANG UNIV
Method for evaluating genetic toxicity of drinking water through multi-genetic-end point biological group tests
InactiveCN105510545AImprove versatilityThe detection method is simpleTesting waterAmes testResearch Object
The invention discloses a method for evaluating the genetic toxicity of drinking water through multi-genetic-end point biological group tests. According to the method, water supply plant filter outflow water and disinfected water serve as research objects, biological group genetic toxicity tests: an Ames test, an in vivo micronucleus test in mouse bone marrow polychromatic erythrocyte, a mouse sperm shape abnormality test, a hamster ovary cell micronucleus test and an SOS / umu test serve as evaluation standards, and when 3 positive results exist in the biological group genetic toxicity test, it is shown that biological genetic toxicity safety exists. The method has the following advantages that three genetic end points of gene mutation, chromosomal aberration and DNA damage are contained; test organisms comprise pronuclei, eukaryon and mammals; in-vivo and in-vitro tests are included; sexual cells and body cells are included; the detection means is relatively simple, convenient, rapid, economical and good in laboratory universality.
Owner:HARBIN INST OF TECH
Health food formula applicable for ovary maintenance, endocrine modulation, anti-oxidation and senility delay of female and method for preparing health food formula
The invention relates to a health food formula applicable for ovary maintenance, endocrine modulation, anti-oxidation and senility delay of female and a method for preparing the health food formula, belonging to the technical field of health food. The health food is refined by adopting soybean isoflavone with natural plant functionality and good moistening function for human body as a main raw material, and porphyrin iron, isomaltose hypgather, biological calcium carbonate, raspberry, vitamin and the like as auxiliary materials. According to the health food in the technology formula, the good effects of the soybean isoflavone on free radical removing and ovary endocrine balance modulation are expressed, the isomaltose hypgather can facilitate the absorption of the soybean isoflavone in the human body, the biological utilization rate of the soybean isoflavone is increased, and thus the intake amount of the soybean isoflavone can be reduced; and simultaneously, the technology formula is also added with the nutrient elements including iron, calcium, vitamin and the like for facilitating the metabolic balance of blood and bone calcium in the human body, thus a senescent process of ovary cells of the female can be slowed down, and the health food formula preparation without side effect is provided for wide female friends.
Owner:WUHAN YIYUANTANG BIOTECH
Antheraea pernyi ovary cell culture medium and application thereof
ActiveCN102618487AComprehensive nutrition for growthEasy to operateArtificial cell constructsVertebrate cellsBiotechnologyPenicillin
The present invention discloses composing components of an antheraea pernyi ovary cell culture medium, a preparation method of the antheraea pernyi ovary cell culture medium, and an application of the antheraea pernyi ovary cell culture medium in antheraea pernyi ovary cell culture. According to the present invention, a prepared amino acid and sugar mixing storage solution (MLM-AAS), an inorganicsalt storage solution (MLM-Salt), a vitamin storage solution (MLM-Vit), bovine serum albumin, fetuin and the like are prepared according to a certain ratio, and the pH value is adjusted to 6.2-6.4 toobtain a MLM-45 culture medium, wherein the prepared MLM-45 culture medium is added with fetal bovine serum and a penicillin and streptomycin mixing solution according to a certain volume ratio before the prepared MLM-45 culture medium is used, the volume ratio of the fetal bovine serum is 20%, and the volume ratio of the penicillin and streptomycin mixing solution is 1%. With a plurality of experiments, the following in vitro cell growth conditions are determined that: the culture temperature is 26-28 DEG C, the pH value is 6.2-6.4, and the osmotic pressure is 315-350 mOsm / kg. With the present invention, technical services are provided for researches in antheraea pernyi biology, antheraea pernyi pathogenic microorganism and related fields.
Owner:辽宁省农业科学院大连生物技术研究所
Separation and in-vitro culture method for pelodiscus sinensis ovary cells
InactiveCN106834207AImprove survival rateNovel stepsArtificial cell constructsGenital tract cellsDigestion TreatmentPhosphate
The invention discloses a separation and in-vitro culture method for pelodiscus sinensis ovary cells. The separation and in-vitro culture method comprises the following steps: (1) selecting sexually immature female pelodiscus sinensis; sterilizing and taking out in-vitro ovary tissues; washing the in-vitro ovary tissues with a phosphate buffering solution and washing; putting the in-vitro ovary tissues into a container; (2) carrying out digestion treatment on the washed in-vitro ovary tissues through adopting collagenase and pancreatin, and centrifuging and collecting the cells; (3) putting the collected cells into a treated cell culture container and adding a complete culture medium for culturing; (4) after the cells are proliferated and fully spread on the bottom of the cell culture container, digesting by utilizing the pancreatin and carrying out subculture or freezing preservation to obtain the pelodiscus sinensis ovary cells. The method disclosed by the invention has novel steps, is simple and convenient and has high repeatability; a pelodiscus sinensis ovary cell line with a high survival rate can be obtained.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
Method for producing pramlintide (PA) by virtue of bombyx mori bioreactor
The invention discloses a method for expressing pramlintide, and belongs to the technical field of genetic engineering. The method is characterized by comprising the following steps: conducting bacterial infection on bombyx mori ovary cells by virtue of a baculovirus multi-gene expression system, so that a baculovirus, which is capable of simultaneously expressing a human-derived amidating enzyme and the pramlintide; and then, injecting the virus into five-instar bombyx mori bodies, so that the amidating enzyme and the pramlintide are co-expressed by the bombyx mori, wherein glycine at the C terminal of the pramlintide is directly amidated by the amidating enzyme, so that the pramlintide, having an activity, is produced, and the pramlintide is purified by virtue of conventional purification means. With the application of the method disclosed by the invention, a plurality of genes can be expressed, which is conducive to co-expression of the human-derived amidating enzyme and the pramlintide; the method disclosed by the invention, which makes use of the bombyx mori bioreactor, is high in protein expression amount and is more complete in modification; and the method disclosed by the invention is short in production time, high in efficiency and low in production cost.
Owner:JINAN UNIVERSITY +2
Improved method used for cloned embryo construction
ActiveCN105039305AEasy to operateImprove efficiencyGenetic engineeringFermentationCytosolic partStaining
The invention belongs to the field of clone technology, and more specifically discloses an improved method used for cloned embryo construction. The improved method comprises following steps: S1, ovocytes are delivered into an operation droplet A, and blind absorption is adopted so as to realize denucleation; S2, cytoplasm obtained via denucleation is delivered into a dyed droplet, dyeing situations are observed directly under a fluoroscope, and the ovocytes processed via denucleation are reserved for standby application; S3, the ovocytes processed via denucleation are delivered into an operation droplet B for standing, and then are delivered into an operation droplet C containing a donor cells, one of the ovocyte is bound with one donor cell, and then is bound with another ovocyte so as to obtain a somatic cell-ovocyte-ovocyte combination; and S4, the somatic cell-ovocyte-ovocyte combination is allowed to stand in a fusion activation fluid, and then is delivered into a fusion slot for electrofusion. The improved method is simple and rapid in operation; denucleation is complete and high in efficiency; and cloning efficiency is high.
Owner:广东中芯种业科技有限公司
Mythimna separata pupa ovary cell line capable of highly yielding baculovirus, and preparation method and applications thereof
ActiveCN112760277AInvertebrate cellsMicroorganism based processesBaculovirus expression vector systemOvary cell
The invention provides a mythimna separata pupa ovary cell line capable of highly yielding baculovirus. The cell line is named as IOZCAS-Myse-1 and assigned the accession number CGMCC No.17282. A construction method of the cell line is provided. The method includes the following steps: step 1) obtaining mythimna separata ovary tissue; step 2) culturing the mythimna separata ovary tissue until a culture flask is filled with proliferative cells, wherein the tissue is closely attached to the bottom of the cell culture flask; and step 3) taking newly proliferative single cells, performing continuous culture until cell passage so as to obtain the cell line. The whole process is conducted under an aseptic condition. The provided cell line can be used for copying baculovirus, so that the baculovirus or baculovirus insecticide can be produced in large scale; and the cell line can also be applied to express the protein having commercial or scientific values by using a constructed baculovirus expression vector system.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
Method for facilitating in-vitro maturation of human immature oocytes by utilizing 3D printing technology
Owner:GUANGXI MEDICAL UNIVERSITY
Superovulation polyvinylpyrrolidone FSH composite slow-release injection for sika deer
InactiveCN105726468AStrong health effectsTrigger detachmentOrganic active ingredientsPeptide/protein ingredientsPyrrolidinonesOvary cell
The invention provides a superovulation polyvinylpyrrolidone FSH composite slow-release injection for sika deer. Four substances, such as FSH, acidic xylosidase, cellulase and gentamicin, are uniformly dispersed in polyvinylpyrrolidone so as to form a slow release agent; and according to a synergistic effect of medicines such as FSH, acidic xylosidase, cellulose, gentamicin and the like, target substances and indirectly produced nutrient substances can continuously and uniformly act on ovary cells so as to promote follicle growth and maturity; and therefore, a good effect on superovulation is achieved, and the mature ovum is high in quality. According to the dosage form, stress reaction is reduced by intravenous injection, and intense struggling caused by pain is alleviated.
Owner:INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
Specific sgRNA for target knocking out human OC-2 genes and application
ActiveCN109112129ASpecific targetingAccurate targetOrganic active ingredientsStable introduction of DNAHuman DNA sequencingOvary cell
The invention provides specific sgRNA for target knocking out human OC-2 genes and application. By adopting the OC-2 genes on a human genome as a target, a series of sgRNA molecules which are obtainedby a series of screening and analysis and can efficiently, specifically and accurately target the human OC-2 gene, as shown in SEQ ID NO.2 to 4. The invention also provides a lentivirus vector and alentivirus comprising the specific sgRNA at the same time. The invention also provides a method for knocking out human OC-2 genes by utilizing a CRISPR-Cas9 system, so that a stable OC-2 gene knockoutstrain can be obtained. The specific sgRNA provided by the invention can block the expression of OC-2 gene in an ovary cell by virtue of a CRISPR / Cas system, the proliferation, migration and invasioncapacity of the ovary cancer cells can be significantly inhibited, a novel direction and concept can be provided for the research and clinical treatment of the ovarian cancer, and the application prospect is good.
Owner:JINAN UNIVERSITY
Macrobrachium nipponense AST gene, primer group for amplification of macrobrachium nipponense AST gene and amplification method thereof
The invention relates to the field of gene cloning, especially to macrobrachium nipponense allatostatin (AST) gene, its amplification method and an amplification primer group. By the use of the primer group provided by the invention, an AST gene full-length cDNA sequence is cloned from macrobrachium nipponense brain tissues. The invention not only provides a theoretical basis for the research of macrobrachium nipponense ecdysis regulation, gonad maturation and oocyte maturation but also establishes a theoretical basis for the improved varieties propagation of macrobrachium nipponense.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
High-yield baculovirus cell line induced by carcinogen, preparation method and application
The invention provides a high-yield baculovirus ovary cells line induced by a carcinogen, a preparation method and an application, a name of the cell line is IOZCAS-Spex12, and a preservation number is CGMCC No. 4804, the preparation method comprises the following steps: culturing beet armyworm ovary cells; and using the carcinogen to induce and converse the beet armyworm ovary cells obtained in the step 1 to obtain the high-yield baculovirus ovary cells line induced and conversed by the carcinogen; and the invention also relates to the application of high-yield baculovirus ovary cells line in baculovirus production.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
Method for detecting decapterus maruadsi oocyte development time phase
PendingCN106367469APaste firmlyGuaranteed timeMicrobiological testing/measurementMaterial analysisYolkDecapterus maruadsi
The invention relates to the field of animals, in particular to a method for detecting a decapterus maruadsi oocyte development time phase. According to the invention, development of oocyte in ovary is researched by adopting a biological tissue section technology. The result shows that a nucleo-cytoplasmic ratio of the oocyte in the ovary is between 0.03 and 0.3, and is obviously reduced for three times totally along with the oocyte development. According to the nucleo-cytoplasmic ratio change of the oocyte, the oocyte development is divided into five stages by virtue of cell volume, nucleus development, and the development conditions of yolk nucleus, follicle cells and the like. Analysis of the area ratio of the oocyte in the ovary at each stage shows that oocytes with different time phases exist in the ovary, so the decapterus maruadsi has the asynchronous spawning characteristic. Compared with the traditional visual-inspection staging method, the histological technique adopted by the research is capable of easily distinguishing the gender, and the staging result is accurate.
Owner:GUANGDONG OCEAN UNIVERSITY
Separation and purification method of Leopard Coral Grouper oogonium and identification and application thereof
PendingCN113774014AIncrease vitalityAvoid damageCell dissociation methodsMicrobiological testing/measurementBiotechnologyZooid
The invention provides a separation and purification method of a Leopard Coral Grouper oogonium and identification and application thereof. By adopting the separation and purification method, the oogonium with the proportion of 85.6-90.3% can be obtained from an ovary cell suspension with the oogonium proportion of about 10% through separation and purification; the method has small damage to the cell, the obtained cell is high in activity, the operation process is simple and convenient, used instruments are common, and the method is easy to popularize and use; according to the identification method of the Leopard Coral Grouper oogonium, a hybridization display technology is adopted, a Nanos2 probe is used for marking the oogonium, and the Leopard Coral Grouper oogonium can be quickly identified; the separation and purification method of the Leopard Coral Grouper oogonium provides a technical guarantee for Leopard Coral Grouper reproductive development research and later amplification of a Leopard Coral Grouper male individual by applying an oogonial stem cells with the help of a germ cell transplantation technology.
Owner:中国海洋大学三亚海洋研究院
Encapsulated cells for treating low testosterone in male subjects
A pharmaceutical composition for treating low testosterone comprises microcapsules the microcapsules containing live mammalian ovary cells. The ovary cells comprise ovarian theca cells in a treatment-effective amount, but not granulosa cells or without granulosa cells in amounts detrimental to the administration of testosterone. Methods of treating male subjects afflicted with low testosterone by administration of such ovary cell-containing microcapsules are also described.
Owner:WAKE FOREST UNIV HEALTH SCI INC
Serum-free medium suitable to culturing ovary cells of Chinese hamster
Owner:EAST CHINA UNIV OF SCI & TECH
Transgenic insect cell line for high-yield baculovirus, and preparation method and application thereof
InactiveCN102807969BMicroorganism based processesViruses/bacteriophagesGenetically modified insectOvary cell
The invention provides a transgenic insect cell line for high-yield baculovirus, and a preparation method and the application thereof. The name of the transgenic insect cell line is IOZCAS-Spex IX and the preservation number of the cell line is CGMCC No.4506. The preparation method of the transgenic insect cell line comprises the steps as follows: spodoptera exigua ovary cells are cultured; human telomerase reverse transcriptase genes are transformed into known expression vectors to obtain recombinant vectors; the recombinant vectors are introduced into the spodoptera exigua ovary cells; and the spodoptera exigua ovary cells are enabled to express human telomerase reverse transcriptase, so as to obtain the transgenic insect cell line. The transgenic insect cell line is applied to the production of the baculovirus.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
Recombinant human adiponectin expression vector, vector construction method and expression method
PendingCN112646837AEfficient expressionIncrease concentrationAntibody mimetics/scaffoldsNucleic acid vectorOvary cellCricetulus
The invention relates to the technical field of biology, in particular to a recombinant human adiponectin expression vector, a vector construction method and an expression method. The recombinant human adiponectin expression vector comprises a nucleotide sequence as shown in SEQ ID No. 1 and an Fc-GS plasmid. The recombinant human adiponectin expression vector construction method comprises the following steps of carrying out codon optimization according to an amino acid sequence of human adiponectin to obtain a cDNA sequence of human adiponectin, wherein the cDNA sequence of the human adiponectin is as shown in SEQ ID No. 1; designing a primer, amplifying the cDNA sequence, and recovering a target fragment after identification; and connecting the target fragment to the Fc-GS plasmid to obtain the recombinant human adiponectin expression vector. When the recombinant human adiponectin expression vector is transfected into hamster ovary cells, recombinant human adiponectin-Fc fusion protein can be stably expressed; and the method is simple, convenient and efficient.
Owner:GENERAL HOSPITAL OF PLA
Construction method, expression method and detection method for protein tag carrier
ActiveCN109402153ABiological material analysisBiological testingGreen fluorescent proteinProtein tag
The invention discloses a construction method, expression method and detection method for a protein tag carrier and relates to the technical field of biomarking. With Drosophila ovarian germ cell specific highly-expressed nos gene taken as an example, a cellular endogenous protein marking technique is developed, namely a protein tagging technique based on terminal exon marking; first, a transgeniccarrier with GFP (green fluorescent protein) tag sequence embedded in the terminal exon is constructed for nos gene; second, Drosophila with the transgenic carrier is prepared by means of mature transgenic technology of Drosophila; whether the expression product of the nos gene in Drosophila ovary cells is subjected to GFP fluorescence expression is detected; the technique herein facilitates theexpression pattern research for model organism Drosophila; references are provided for mammalian gene expression and positioning.
Owner:ANHUI NORMAL UNIV
Compositions and methods relating to ovary specific genes and proteins
The present invention relates to newly identified nucleic acids and polypeptides present in normal and neoplastic ovary cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions comprising the nucleic acids, polypeptides, antibodies, variants, derivatives, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating ovarian cancer and non-cancerous disease states in ovary tissue, identifying ovary tissue, monitoring and identifying and / or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered ovary tissue for treatment and research.
Owner:SALCEDA SUSANA +5
Orient armyworm pupal ovary cell line with high production rate of baculovirus and its preparation method and application
ActiveCN112760277BInvertebrate cellsMicroorganism based processesBaculovirus expression vector systemOvary cell
The present invention provides a high-yielding baculovirus pupae ovary cell line of Oriental Armyworm. The name of the cell line is IOZCAS-Myse-1, and its preservation number is CGMCC No.17282. The present invention provides a method for constructing the O. orientalis pupal ovary cell line of the high-yielding baculovirus. The method comprises the following steps: step 1) obtaining the ovary tissue of the oriental armyworm; step 2) cultivating the ovary tissue of the oriental armyworm, until The proliferated cells are filled with the culture flask, wherein the tissue is closely attached to the bottom of the cell culture flask; step 3) take the newly proliferated single cell and continue to culture until the cell is passed down; the whole process is carried out under sterile conditions . The oriental armyworm pupal ovary cell line with high yield of baculovirus provided by the present invention can be used to replicate baculovirus for large-scale production of baculovirus or baculovirus insecticide; the cell line can also be applied to construct Baculovirus expression vector system for expressing proteins of commercial or scientific value.
Owner:INST OF ZOOLOGY CHINESE ACAD OF SCI
Ovary egg cell repairing composition and preparation method thereof
PendingCN113730558AImprove balancePromote blood circulationOrganic active ingredientsPeptide/protein ingredientsBiotechnologyChamomile extract
The invention relates to the technical field of ovarian repair and discloses an ovary egg cell repairing composition and a preparation method thereof. The invention aims to solve technical problems that an existing ovary aging treatment method has a large side effect and an unclear curative effect. The invention provides an ovary egg cell repairing composition. The composition comprises a motherwort herb extract, a kudzuvine root extract, a rhizoma belamcandae extract, a peony root extract, a seaweed extract, a yeast extract, an apple fruit cell culture extract, a golden chamomile extract, a hamamelis virginiana extract, royal jelly freeze-dried powder, collagen, soy isoflavone, xanthan gum, carbomer, disodium glycyrrhizinate, chitosan and a coenzyme A. The invention further provides a preparation method of the ovary egg cell repairing composition. The composition can promote female hormone balance, regulate female reproductive system, promote pelvic blood circulation, enhance ovary immunity, enable ovary to secrete healthy ova, and achieve a purpose of effectively repairing egg cells in ovary.
Owner:广东圆康再生医学科技开发有限公司
Compositions and methods relating to ovarian specific genes and proteins
InactiveUS20050148008A1Peptide/protein ingredientsGenetic material ingredientsDiseaseAntiendomysial antibodies
The present invention relates to newly identified nucleic acids and polypeptides present in normal and neoplastic ovary cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions comprising the nucleic acids, polypeptides, antibodies, variants, derivatives, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating ovarian cancer and non-cancerous disease states in ovary tissue, identifying ovary tissue, monitoring and identifying and / or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered ovary tissue for treatment and research.
Owner:SALCEDA SUSANA +7
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