Method for detecting decapterus maruadsi oocyte development time phase

A technology of egg cells and oocytes, which is applied in the field of detection of the developmental phase of blue trevally eggs, can solve the problems of low probability of success of the experiment, failure to consider nucleolus distribution and number changes, nucleocytoplasmic connections, etc.

Pending Publication Date: 2017-02-01
GUANGDONG OCEAN UNIVERSITY
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Problems solved by technology

Although this staging method has the characteristics of simplifying the number of stages and being easy to understand and remember, it does not take into account the distribution and number changes of nucleoli and the connection between nucleoplasm, and because follicular cells are located around the cells, it is easy to stain. Washed away by the staining solution, the probability of success in the experiment is low

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  • Method for detecting decapterus maruadsi oocyte development time phase
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  • Method for detecting decapterus maruadsi oocyte development time phase

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Experimental program
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Effect test

Embodiment 1

[0137] Sample source:

[0138] The blue squid samples in the breeding season came from small fishing boats in Beihai Qiaogang, Wenchang Qinglan Wharf, and Yangjiang Zhapo Wharf in the Beibu Gulf. The sampling time was spring, and 100 samples were collected from each location. When collecting samples, record the collection location, number and fishing area (see Table 1).

[0139] Table 1 Basic information of sample collection

[0140]

[0141] Gonad sample sampling and processing methods:

[0142] The samples taken at the wharf were taken back to the laboratory after adding ice to the blue round trevally, and the gonads were taken out in time for relevant measurements, and some gonad samples were soaked in Born's solution for preservation, and some were directly stored at low temperature.

[0143] Optical microscope slide production process:

[0144] (1) Preparation: Soak the required slides and coverslips in pure water added with washing powder or detergent for one night...

Embodiment 2

[0161] Egg cell development phase of blue round trevally:

[0162] Through the histological observation of the samples, according to the development of egg cell nucleolus, yolk nucleus, oil droplet, yolk granule and the change of nucleus-to-cytoplasmic ratio of egg cells, the development process of the egg cell of the blue round trevally can be divided into five phases: the first phase ( Oogonia stage), the second phase (the small growth period of the primary oocyte), the third phase (the initial stage of the primary oocyte growth), the fourth phase (the late stage of the primary oocyte growth), the first phase Phase 5 (secondary oocyte stage).

[0163]Stage I: The stage of oogonia, whose shape is triangular, round and oval, and the cell membrane is not easy to distinguish. The cells in this stage are small in size, with a long diameter of 7.6 microns and a short diameter of 4.6 microns. The oogonia are dispersed, and the intercellular connections are small. Inside the oogon...

Embodiment 3

[0192] For the traditional visual staging method of gonad maturity of marine fish, see "Marine Survey Specifications-Marine Biological Survey" (GB / T12763.6-2007). The gonadal development of almost all marine fishes is divided according to this staging method, which is universal, but lacks specificity for each species. Blue round trevally can be divided into 6 stages according to the apparent color of the gonads and the proportion of the abdominal cavity space, which is highly subjective and difficult to grasp the judgment standard; at the same time, the traditional visual staging method cannot distinguish the gonad maturity stage I Individual sex, because the appearance characteristics of stage I gonads are not significantly different between males and females.

[0193] This method divides the gonad maturity of blue round trevally, and the judgment standard of each development stage is digitized, which reduces the subjective influence of the researcher and improves the accuracy ...

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Abstract

The invention relates to the field of animals, in particular to a method for detecting a decapterus maruadsi oocyte development time phase. According to the invention, development of oocyte in ovary is researched by adopting a biological tissue section technology. The result shows that a nucleo-cytoplasmic ratio of the oocyte in the ovary is between 0.03 and 0.3, and is obviously reduced for three times totally along with the oocyte development. According to the nucleo-cytoplasmic ratio change of the oocyte, the oocyte development is divided into five stages by virtue of cell volume, nucleus development, and the development conditions of yolk nucleus, follicle cells and the like. Analysis of the area ratio of the oocyte in the ovary at each stage shows that oocytes with different time phases exist in the ovary, so the decapterus maruadsi has the asynchronous spawning characteristic. Compared with the traditional visual-inspection staging method, the histological technique adopted by the research is capable of easily distinguishing the gender, and the staging result is accurate.

Description

technical field [0001] The invention relates to the field of animals, in particular to a method for detecting the developmental phase of the egg cells of the blue round trevally. Background technique [0002] Because different fish have different genetic characteristics and living environment conditions, the development of their ovaries and egg cells is also different. So far, there is no consistent standard for the staging of ovaries and oocytes at home and abroad. [0003] In 1939, Soviet scholar Мейен divided fish gonads into 6 phases. This division has been recognized by many scholars and cited by most scholars. However, the classification basis used by Мейен is only the proportion of the number of oocytes in each stage. This method does not consider oogonia, which is not systematic, and because the volume of oocytes in each stage varies greatly, the number of oocytes is not comparable. sex. [0004] In 1964, Shi Tengfang divided the gonads of silver carp into four s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02
CPCG01N33/4833
Inventor 李忠炉崔黎军高兵兵颜云榕卢伙胜
Owner GUANGDONG OCEAN UNIVERSITY
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