500 results about "Spodoptera" patented technology

Disclosed and claimed is a new insect cell line, Sf900+, ATCC CRL-12579. The insect cell line was established from Lepidoptera, Noctuidae, Spodoptera frugiperda Sf-9 (ATCC CRL-1711) through multiple rounds of limiting dilution and selection in a serum-free insect medium supplemented with added human insulin. The insect cell line is useful in BEVS or as an adjuvant and has many characteristics and advantages. Also disclosed and claimed are recombinant proteins from recombinant baculovirus expression in insect cells such as Sf900+ cells, for instance, HA, NA, EPO, CD4, CEA, and thrombospondin.

The subject invention relates in part to stacking Cry1Be toxins along with Cry1Fa toxins to prevent insects from developing resistance towards either toxin by itself. As discussed in more detail herein, the subject pair of proteins is a particularly advantageous combination, as no other pair of proteins is known to provide high levels of control and non-cross-resistant activity against both Spodoptera frugiperda (FAW) and Ostrinia nubilalis (ECB) insects. This dual, non-cross-resistant activity is also advantageous because it can reduce the number of proteins / genes needed to target these insects with multiple, non-cross-resistant proteins. This can reduce or eliminate the need for refuge acreage. Accordingly, the subject invention also relates generally to using four genes to provide three proteins for non-cross-resistant control of a first insect, and three proteins for non-cross-resistant control of a second insect. In preferred embodiments, the targeted insects are FAW and ECB.

Disclosed and claimed is apparatus and methods for the growth of cells to high density, products therefrom and uses thereof. Also disclosed and claimed is the use of this method for the growth to high-density insect cells, such as the Spodoptera frugiperda Sf900+ cell line (ATCC: CRL 12579). Further disclosed is the infection of Sf900+ cells at high cell density with wild type and recombinant baculoviruses to produce baculovirus and DNA or gene or expression products.

The invention belongs to the field of pesticide, which mainly relates to an ultralow-volume solvent containing pleocidin or spinetoram. The ultralow-volume solvent is an active component I or an active ingredient compounded by the active component I or an active component II, and the balance is solvent and accessory ingredient, wherein the active component I is pleocidin or spinetoram; and the active component II is insecticide of the other type of action mechanism. The ultralow volume solvent is simple to process and has the advantages of water conservation, high work efficiency, long efficacy continuing period, small possibility of generating resistance to drug for a target, small environment pollution, synergy and the like. Ultralow volume spraying, low-volume spraying or low-volume electrostatic spraying is applied to effectively prevent and control various pests, such as Cnaphalocrocis medinalis, Chilo suppressalis, rice planthopper, Ostrinia nubilalis, plutella xylostella, cabbage caterpillar, Spodoptera litura, Spodoptera exigua, aphids, Lesser green leaf hopper, Icerya purchase, aleyrodid, Thrip, stinkbug, acridid, flea beetle, leafminer, looper and the like.

The invention relates to the field of pesticides, in particular to ultralow volume liquid containing benzoylurea insecticide, which comprises active ingredients, solvent and adjuvant. The active ingredient I is optional one of lufenuron, diflubenzuron, triflumuron, chlorfluazuron and chlorbenzuron belonging to benzoylurea, and the active ingredient II is insecticide of another type of function mechanism. The ultralow volume liquid containing benzoylurea insecticide has the advantages of simplicity in processing, high efficiency, water saving, long-term effect, a target being not easy to resist the insecticide, low environmental pollution, synergy and the like. Application of ultralow volume spray, low volume spray or ultralow volume static spray is well effective in controlling ostrinia furnacalis, sugarcane borer, locust, peach fruit borer, lesser green leaf hopper, striped flea beetle, bean fly, diamond back moth, prodenia litura, cabbage caterpillar, leaf beetle, thrip, leaf miner, inchworm, red spider and the like, and the ultralow volume liquid containing benzoylurea insecticide can be used for effectively controlling various pests on wheat, maize, vegetables, tobacco plants, tea plants, fruit trees, cotton plants, woods and sugarcanes.

The invention discloses a fluoro methoxylpyrazole-containing o-formylaminobenzamide compound shown as a structural formula (I) or agriculturally suitable salts thereof, a synthesis method and application thereof. The fluoro methoxylpyrazole-containing o-formylaminobenzamide compound has the advantages of effectiveness, low toxicity, low cost and environmental safety and can be used for preventingand removing various pests in the fields of agriculture and horticulture or pests parasitized on animals. The fluoro methoxylpyrazole-containing o-formylaminobenzamide compound has very high activityon lepidopteran pests (plutella xylostella, spodoptera exigua and cotton bollworm), hemipteran pests (black peach aphid and aphis craccivora koch), homopteran pests (nilaparvata lugens), dipteran pests (liriomyza trifolii) and leaf beetle pests (phaedon conchleariae). Particularly, good effect on the lepidopteran pests, the hemipteran pests and the dipteran pests can be achieved under the condition of low dosage of the compound.

The present invention discloses an active biological drug fertilizer, which is characterized in that: the present invention is prepared with the fermenting process by photosynthetic bacteria, yellow corrupted acid and the extraction from Azadirachtin, Langdu Root and kusnezoff monkshood. The present invention is with an especial function for killing insect and preventing disease as well as accelerating the growth for the plant, protecting flower and fruit, enhancing the output and improving the integrative performance for the product quality and so on. The present invention is adaptable to the crops including rice, wheat, cotton, cole, bean, earthpea, corn, bast fibre plants, watermelon, fruit tree, vegetable, tobacco leaf, tea leaf, medicinal materials, flower, and pasture and seeding wood and so on. Besides, the present invention has also the function for accelerating the output for the pecan. The prevention and cure objects include: lepidopterans, orthopteran, Diptera, Coleoptera, ring vein, Hymenoptera and acarid and so on. The present invention has the much higher sensitivity to the following pests including: prodenia litura, asparagus caterpillar, cabbage butterfly, tea geometrid, tea smaller green leaf hopper, pecan peachblossom maggot and locust and so on.

The invention discloses a sex pheromone composite for luring prodenia litura and a luring core thereof. The sex pheromone composite is characterized in that: the sex pheromone composite consists of the following components of sex pheromone and phenylacetaldehyde, wherein the sex pheromone consists of cis-9,trans-11-tetradecadienoic acetate and cis-9,trans-13-tetradecadienoic tetradecadienoic which are mixed in a mass ratio of 3-13: 1; and the phenylacetaldehyde is used as a synergist and accounts for 20 to 60 percent of the total mass of the components. The invention also discloses a related luring core. The researches of the invention prove that the phenylacetaldehyde can greatly improve the technology of luring and killing prodenia litura by sex pheromone, solves the problem of low control efficiency caused by multiple mating of the prodenia litura in the prior art, plays an important role in and has a great significance for the control, detection and reporting of the sexual luring insects such as the prodenia litura.

The invention relates to a method for controlling spodoptera litura. The method for controlling the spodoptera litura comprises the step that the spodoptera litura is made to make contact with CrylA protein. According to the method for controlling the spodoptera litura, the spodoptera litura is controlled through the CrylA protein which is generated by bodies of plants and is capable of killing the spodoptera litura. Compared with an agricultural control method, a chemical control method and a physical control method adopted in the prior art, the method for controlling the spodoptera litura carries out protection on all growth periods and all plants to control the infringement of the spodoptera litura, and is free of pollution and residues, stable and thorough in effect, simple, convenient to implement and economical.

The invention discloses a substituent ether compound which is structurally shown as a general formula (I): the definition of each substituent group refers to an instruction manual. The compound has the broad-spectrum insecticidal activity, is very effective to lepidoptera pests including European corn borers, sugarcane borers, summer fruit tortrix moths, apple budworms, tussock moths, rice leaf rollers, corn borers, tobacco leaf worms, small budworms, cabbage moths, lesser corn worms, cotton leaf worms and the like, particularly has better activity to the cabbage moths and the lesser corn worms and can obtain good effect under the low dosage. The substituent ether compound also has high activity to homoptera pests like aphids and the like. Meanwhile, a part of compound also has good bactericidal activity and can be used for preventing and treating blumeria graminis, cucumber downy mildew, vegetable gray mold and the like.

The invention relates to prodenia litura artificial feed, which is prepared from the following raw materials in weight percentage: 5-10% of soybean flour, 1-5% of wheat germ flour, 1-5% of yeast powder, 1-5% of agar, 0.1-0.5% of corrosion preventive, 0.1-0.5% of mold preventive, and the balance of water. The invention further relates to a preparation method of the artificial feed and a method for raising prodenia litura. The method for raising the prodenia litura is simple, convenient and practical to operate, reduces feed replacing frequency and disease infection, and can obtain large amount of prodenia liturae with identical age.

The invention belongs to the pesticide field and particularly relates to a synergic pesticidal composition with active ingredients to be the combinations of fipronil and a series of subspecies of Bacillus thuringiensis (Bt for short), wherein the Bacillus thuringiensis subspecies include six subspecies: Bacillus thuringiensis var.kurstaki, Bacillus thuringiensis var.aizawa, Bacillus thuringiensis var.tianmensis, B.thuringiensis var.israelensis, Bacillus thuringiensis subsp.aizawai and Bacillus thuringiensis subsp. kenyae. The main agent thereof includes the following active ingredients by the weight percentages as follows: fipronil 0.1%-80%, Bacillus thuringiensis 100-100000 IU (international unit) / mg, or 10-1000 billion spore / g, or 0.01-20% toxin protein, and the rest is an accessory ingredient. The pesticidal composition can be efficiently used for controlling agricultural pests such as rice leaf rollers, chilo suppressalis walkers, diamondback moths, prodenia lituras, corn borers, and so on, and has particular characteristics of high efficacy, long lasting period and safe usage, so that the pesticidal composition belongs to environment-friendly pesticides and can prolong the generation of resistance, reduce production cost and increase economic benefit.

The invention discloses an insect-fusion-resistant gene, coding protein, a carrier and application thereof. The insect-fusion-resistant gene is formed by using a connecting peptide coding gene to connect a nucleotide sequence of coding BT insecticidal crystal protein Cry1Ab with coding BT trophophase insecticidal protein Vip3A, the connecting peptide amino acid sequence is shown as SEQ ID NO:1. Insecticidal activity of the insect-fusion-resistant gene is improved, and insecticidal spectrum is wider. The insect-fusion-resistant gene can kill main lepidopterous insects like armyworm, Spodoptera litura, Ostrinia furnacalis, cotton bollworm and Spodoptera exigua of paddy and maize. In addition, the insect-fusion-resistant gene can effectively slow down insect resistance.

The invention relates to a planting technology of purple sweet potatoes, in particular to various measures for planting purple sweet potatoes on the aspects of purple sweet potato seed selection, fertilization, seedling culturing, field management and the like. The planting technology comprises the following steps of: selecting Japanese king purple sweet potatoes serving as seeds; taking the seeds out of a cellar when the temperature is about 15 DEG C in the middle of February for seedling culturing; transversely arranging the seeds on a seedling bed continuously at the line space about 5 centimeters; covering with fine soil; spraying water onto the soil with a sprayer; covering with a plastic film; preserving moisture and heat; when seedlings grow to over 20 centimeters, inserting the seedlings into a field; performing deeply digging and ridging on a sun-exposed plot with a high relief, wherein the ridge distance is generally 60-70 centimeters, and the ridge height is 25-30 centimeters; performing top application once to twice according to the soil fertility; at the tuber expansion stage, keeping the ground wet and keeping the soil moisture at about 70-80 percent; and at the field growing period, selecting low-toxicity chemical pesticides in time to prevent and control pests such as aphids, palaearctic sweet potatoes, prodenia lituras and the like.

This invention relates to a process for preventing or delaying the development of resistance in populations of Spodoptera frugiperda to transgenic plants expressing a Cry1A and / or a Cry1F protein, comprising providing such plants also with a gene expressing a VIP3 protein, as well as to related uses and methods, such as methods for the production of transgenic plants comprising two different insecticidal proteins that show no competition for binding to the binding sites in the midgut brush border of Spodoptera frugiperda larvae.

The invention belongs to the technical field of researches and the development of attractants for injurious insects, and particularly relates to a spodoptera frugiperda attractant composition. The spodoptera frugiperda attractant composition comprises a sex pheromone and an auxiliary ingredient, and the sex pheromone comprises (Z)-7-dodecene-1-farnesyl acetate, (Z)-9-tetradecene acetate, (Z)-11-hexadecyl acetate and (Z)-9-dodecylene-1-farnesyl acetate. The attractant composition can be used for trapping spodoptera frugiperda.

The invention discloses paecilomyces javanicus strain and the application thereof. The strain adopts paecilomyces javanicus IJ-N2, the strain is preserved in the China Center for Type Culture Collection on tenth of June in 2008 with a CCTCC No of M208085. The strain is wild strain obtained through separating a plutella xylostella body naturally infested by entomogenous fungous from Nonggang Natural Forest Reserve Areas in Guangxi, the paecilomyces javanicus strain is obtained through the wild strain being back inoculated to the plutella xylostella body for rejuvenation, and depurative strain is obtained through the monospore separating operation being performed to the strain. The paecilomyces javanicus strain has strong infestation and insecticidal effect to aleyrodids, solenopsis invicta buren, spodoptera litura, aphid and plutella xylostella according to long-term infection biology study and indoor bioassay.

The invention discloses a Bacillus thuringiensis MB-15 strain which has a broad spectrum and high virulence on vegetable lepidoptera pests, and the preservation number of the strain is CGMCC No. 5255. The strain can form a diamond insecticidal crystal protein, contains cry1I, cry1Ac, cry2Ac and vip3Aa genes, and has high-efficiency insecticidal activity on plutella xylostella, Pieris brassicae, beet armyworm, Spodoptera litura, Helicoverpa armigera and other lepidoptera pests. The invention also relates to a preparation method of the wettable powder of strain. The wettable powder comprises the following content of main components: 40.0-50.0% of Bacillus thuringiensis MB-15 strain powder, 5.0-10.0% of a dispersant, 5.0-10.0% of a wetting agent, 0.5-1.0% of a stabilizer, 0.5-1.0% of an ultraviolet light protection agent, and 28.0-49.0% of a carrier. The wettable powder can be used to control a plurality of lepidoptera pests on vegetables, and has the characteristics of broad spectrum, high efficiency, and safety, etc.

Disclosed is an indoor artificial reproduction method of monoparasitism apanteles ruficrus haliday. The method includes the steps of host breeding and bee breeding; host breeding refers to taking prodenia litura larvae of two to three years old as reproduction hosts, transferring part of the larvae into an environment of 6 DEG C for breeding to be old larvae for pupation, and forming generation overlapping of host larvae; bee breeding includes bee inoculating, cocoon management, adult bee management the like, bee inoculating refers to placing leaves with traces formed by gnawing of the host larvae and five healthy prodenia litura larvae of two to three years old in a flat-bottom test tube of 2x10cm for inoculating for 2 hours, and cocoon and adult bee management needs to be performed under relatively proper conditions and needs to be matched with corresponding management technology. The indoor artificial reproduction method is easy to get in materials, high in reproduction rate and high in stability.

The invention discloses a phytotron for feeding prodenia litura and an indoor artificial breeding method. The method comprises the following steps: putting prodenia litura larvae into the phytotron under a specific condition; selecting natural hosts (tobacco leaf, Chinese cabbage and the like) to feed the prodenia litura larvae; changing fresh hosts everyday and removing faeces and rotten leaves; after the prodenia litura larvae pupate, centrally putting the prodenia litura into an insect rearing box; after adult emergence of the prodenia litura, pairing the prodenia litura according to the female-male ratio of 1 to 1, laying eggs and breeding population. According to the feeding method, the prodenia litura are fed in the manmade phytotron, the feeding technique is simple and easy to operate, low in feeding cost, and high in reproductive rate, and standard health test insects can be obtained in an indoor continuous feeding manner.

The invention discloses an Isaria javanicanicus mould strain and application thereof. The high-efficiency Isaria javanicanicus (Friedrichs & Bally) strain Ij01 is classified to belong to Corynespora, Hyphomycetaceae, Hyphomycetales, Hyphomycetes, Mitosporic Fungi, Eumycota. The strain has been preserved in China Center for Type Culture Collection (CCTCC), the preservation date is Oct 27, 2008, and the preservation number is CCTCC M 208171. The high-efficiency Isaria javanicanicus mould is entomopathogenic fungi to have high efficiency for preventing and controlling prodenia litura, has the characteristics of brand-new action mechanism different from the prior chemical insecticides, and no environmental pollution and residue when taken as a living biological pesticide, and meets the requirement of organic food production.

The invention discloses an artificial group breeding method of prodenia litura (fabricius). Larva and a proper number of fodder are placed in plastic boxes which are respectively 11.5cm, 13.5cm and 16.5cm in diameter and are sterilized to be bred, the plastic boxes are covered by gauzes, the box with 1 to 3 old larva is tightly covered by a lid or the mouth of the box with 4 old larva is bonded by a rubber band, the fodder and the plastic boxes are replaced every day, and after the larva pupate, pupas are picked out from the fodder and placed in the plastic boxes, and the pupas are kept wet by degreasing cotton; male and female eclosion adults are paired and placed in a glass cup to be bred, and the adults generate experimental groups under certain breeding conditions. The method is simple, convenient to perform, low in breeding cost and good in breeding effect and can perform indoor transgenerational breeding.

The invention discloses an in vivo reproduction method of entomopathogenic nematodes, relating to an in vivo culture method of entomopathogenic nematodes and aiming to solve the problems of low yield in applying the tenebrio molitors to breed the nematodes. The in vivo reproduction method comprises the following steps of: firstly, cutting two pieces of medium speed qualitative filter paper and filling in a cover of a culture dish A; then placing prodenia litura larvae; then soaking the medium speed qualitative filter paper by using water suspension infective juveniles; covering the bottom of the culture dish A to the cover of the culture dish A till all prodenia litura larvae die; removing the bottom of the culture dish A; placing the cover of the culture dish A in a culture dish B; filling sterile water to a gap between the culture dish B and the culture dish A; culturing at a constant temperature till the infective juveniles occur in the sterile water; then collecting the sterile water with the infective juveniles every day; and complementing the sterile water so as to complete the in vivo reproduction of the entomopathogenic nematodes. The in vivo reproduction method of the entomopathogenic nematodes is mainly used for the in vivo reproduction of the entomopathogenic nematodes.

The invention discloses artificial feed for Spodoptera frugiperda larvae and a preparation method and application thereof. The artificial feed for Spodoptera frugiperda larvae is made from, by weight,200-300 parts of corn meal, 80-120 parts of soybean meal, 10-30 parts of yeast powder, 10-20 parts of milk powder, 5-15 parts of vitamin complex, 6-9 parts of ascorbic acid, 2-6 parts of methyl 4-hydroxybenzoate, 2-5 parts of sorbic acid, 10-30 parts of agar powder, and 1500-2000 parts of sterile water. The invention also provides a method of feeding with the artificial feed. The artificial feedfor Spodoptera frugiperda larvae and the preparation method and application thereof have the advantages that materials are easy to attain, the cost is low, preparation is convenient, storage time is long, nutrition is complete, feeding effect is good, the artificial feed is very practical, and large-scale feeding of Spodoptera frugiperda larvae is benefited.

The invention relates to an application of an insecticidal protein. A method for controlling prodenia litura comprises the step of enabling the prodenia litura to contact Cry2Ab protein at least. The prodenia litura is controlled and killed by Cry2Ab protein generated inside plants. Compared with agricultural control methods, chemical control methods and physical control methods in the prior art, the insecticidal protein can protect the wholes plants in the whole growth period against the attack of prodenia litura, is free from pollution and residue, is stable and thorough in effect, is simple and economical and is convenient to use.

The invention discloses an insecticidal composition containing sulfoxaflor, comprising A and B components, wherein the active component A is selected from sulfoxaflor and the active component B is selected from any one of the following insecticides: pymetrozine, imidacloprid, acetamiprid, nitenpyram, thiamethoxam, buprofezin, thiacloprid, clothianidin and dinotefuran; and the weight percentage of the active components A to B is: (1-50%): (1-50%). The composition can be used for preventing and treating many insects, has obvious synergy, expands the insecticidal range and has relatively high activity on sucking insects such as plant hopper, aphid, Empoasca pirisuga Matsumura and aleyrodid, and Lepidoptera pests such as plutella xylostella and spodoptera exigua. Furthermore, the composition reduces the usage of the farm chemical and residue of the farm chemical on the crops, alleviates the environment pollution, and has safety on both human and poultry, good environment compatibility; and the insects are difficult to generate drug resistance.

The invention relates to a pesticide composition with a synergy effect. The pesticide composition is characterized in that effective components comprise methoxyfenozide and emamectin benzoate, wherein the mass ratio of the methoxyfenozide and the emamectin benzoate is 100:1-1:1. The composition has obvious synergy effect and can reduce the pesticide dosage and delay the resistance development. The composition can be used for preventing and controlling lepidoptera pests and is particularly suitable for preventing and controlling noctuidae pests, such as spodoptera exigua, prodenia litura, cotton bollworm, and the like.