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Fungi Imperficti strain and uses thereof

A kind of Paecilomycetes, Paecilomyces vulgaris technology, applied in the application, fungi, biocides and other directions

Inactive Publication Date: 2009-01-14
GUANGDONG XINJINGXIANG BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prevention and control of pests such as fire ants, Spodoptera litura, whitefly and diamondback moth, traditional chemical control methods are mostly used. In terms of biological pesticide control, there is no relevant domestic use of Paecilomyces javanica strains for the control of the above pests. patent application

Method used

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  • Fungi Imperficti strain and uses thereof
  • Fungi Imperficti strain and uses thereof
  • Fungi Imperficti strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Isolation, purification, identification and screening of Paecilomyces javanica of embodiment 1

[0014] 1.1 Materials

[0015] The carcass of a diamondback moth Plutella xyostella infested by fungus fungus was collected from vegetables in Nonggang Nature Reserve, Guangxi Province.

[0016] Czapek-Dox: NaNO 3 2g,K 2 HPO 4 1g, KCl 0.5g, MgSO 4 0.5g, FeSO 4 0.01g, 30g sucrose, 20g agar, add water to make the total volume 1000ml and boil it, then sterilize by autoclaving at 121°C for 30min.

[0017] Aseptic operating conditions: All utensils and utensils must be sterilized by autoclaving (121°C, 30min), and operations such as inoculation are carried out in ultra-clean workbenches.

[0018] Culture conditions: culture in a 25°C light (14L:10D) incubator, transfer to a PDA slope after colony formation, and then transfer to a 4°C refrigerator for storage.

[0019] 1.2 Isolation and identification of pathogenic bacteria

[0020] (1) Isolation, purification and ident...

Embodiment 2

[0060] Embodiment 2 Paecilomyces javanica IJ-N2 bacterial strain is to the determination of the pathogenicity of red fire ants

[0061] Bioassay is one of the effective means to detect the degree of lethality and lethality rate of entomogenic fungi to target pests, and can provide an important reference for comprehensive evaluation of the biological control potential of the fungus. In the invention, the pathogenicity of the Paecilomyces javanica IJ-N2 strain to the red fire ants is measured, so as to screen out the optimal concentration used in the prevention and control.

[0062] Insects to be tested: red fire ants, collected from the lawn of Guangzhou University Town, raised indoors for a period of time after collection, and selected healthy large worker ants of the same size for the test after the population stabilized.

[0063] (1) Treatment of test strains

[0064] The Paecilomyces javanica IJ-N2 bacterial strain that obtains after purification, adopts Czapek plate, in t...

Embodiment 3

[0073] Embodiment 3 Paecilomyces javanica IJ-N2 bacterial strain is to the determination of the pathogenicity of Spodoptera litura larvae

[0074] Insects and host plants for testing: Spodoptera litura, the adults of Spodoptera litura raised on kale in the net room were inoculated onto the kale seedlings without insects, and after 12 hours of oviposition, the adults were removed, and the kale seedlings were placed in In a light incubator at 25±0.5°C, wait until Spodoptera litura develops to the 2nd instar larvae. Brassica campestris L. was purchased from Vegetable Research Institute of Guangdong Academy of Agricultural Sciences, and potted seedlings with 8-10 expanded leaves were used for the test seedlings.

[0075] (1) Treatment of test strains

[0076] The Paecilomyces javanica IJ-N2 bacterial strain obtained after purification was cultivated for 10 days in a thermostat (14L: 10D) at 25 ± 0.5°C on a Czapek plate, and the sterile water of 0.03% Tween-80 was added to collect...

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Abstract

The invention discloses paecilomyces javanicus strain and the application thereof. The strain adopts paecilomyces javanicus IJ-N2, the strain is preserved in the China Center for Type Culture Collection on tenth of June in 2008 with a CCTCC No of M208085. The strain is wild strain obtained through separating a plutella xylostella body naturally infested by entomogenous fungous from Nonggang Natural Forest Reserve Areas in Guangxi, the paecilomyces javanicus strain is obtained through the wild strain being back inoculated to the plutella xylostella body for rejuvenation, and depurative strain is obtained through the monospore separating operation being performed to the strain. The paecilomyces javanicus strain has strong infestation and insecticidal effect to aleyrodids, solenopsis invicta buren, spodoptera litura, aphid and plutella xylostella according to long-term infection biology study and indoor bioassay.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Paecilomyces javanica and its application in pest control. Background technique [0002] In the prevention and control of pests such as fire ants, Spodoptera litura, whitefly and diamondback moth, traditional chemical control methods are mostly used. In terms of biological pesticide control, there is no relevant domestic use of Paecilomyces javanica strains for the control of the above pests. patent application. [0003] Paecilomyces javanica is an entomopathogenic fungus. As a living biological pesticide, it has a new mechanism of action different from existing chemical insecticides, has no pollution to the environment, and has no residue. Alternative control drugs for Bacillus (Bt) resistant insects. Contents of the invention [0004] An object of the present invention is to overcome the deficiencies of the prior art and provide a Paecilomyces javanica s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/79
Inventor 黄振任顺祥
Owner GUANGDONG XINJINGXIANG BIOLOGICAL ENG CO LTD
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