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Method for facilitating in-vitro maturation of human immature oocytes by utilizing 3D printing technology

A 3D printing and oocyte technology, applied in the field of biological 3D printing, can solve problems such as poor mood, cancer risk, ovarian hyperstimulation syndrome, etc., and achieve the effect of reducing technical costs, fast and convenient process, and high degree of automation

Inactive Publication Date: 2017-04-19
GUANGXI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present in this patented method allows us to create an organism-like structure called a follophery by making tiny droplets containing cells directly onto paper or other materials like plasterboard instead of growing them into eggs. This results in faster and more accurate cell culture than previous methods because it uses 3D printers rather than traditional techniques such as egg shell production.

Problems solved by technology

This patented method involves inducing stereology during natural or induced abortion procedures that can help prevent conception failures caused by insufficient estrogen levels at menopausal ages. Current methods require expensive equipment like sophisticated techniques involving multiple steps before successful births occur due to their complexity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for promoting in vitro maturation of human immature oocytes using 3D printing technology, the method comprising the following steps:

[0024] (1) Select ovarian cells with a diameter between 50 and 60 μm, wash them with normal saline, and collect naked eggs and granulosa cells digested with digestive enzymes to form ovarian cells;

[0025] (2) Put the in vitro maturation culture medium in the culture dish, then put the ovarian granulosa cells, cover with mineral oil, and put them in a CO2 incubator for balanced culture for 24 hours. The culture temperature is 37°C, the culture humidity is saturated humidity, and the outside air The environment contains 5.0% CO2;

[0026] (3) Pick out the apoptotic oocytes, with each droplet containing 15 oocytes as the standard, put the droplet into the ovarian granulosa cells after culturing for 24 hours, and continue culturing for 2 days at a temperature of 37°C, the cultivation humidity is saturated humidity, and the extern...

Embodiment 2

[0034] A method for promoting in vitro maturation of human immature oocytes using 3D printing technology, the method comprising the following steps:

[0035] (1) Select ovarian cells with a diameter between 50 and 60 μm, wash them with normal saline, and collect naked eggs and granulosa cells digested with digestive enzymes to form ovarian cells;

[0036] (2) Put the in vitro maturation culture medium in the culture dish, then put the ovarian granulosa cells, cover with mineral oil, and put them in a CO2 incubator for balanced culture for 24 hours. The culture temperature is 37°C, the culture humidity is saturated humidity, and the outside air The environment contains 5.0% CO2;

[0037] (3) Pick out the apoptotic oocytes, with each droplet containing 15 oocytes as the standard, put the droplet into the ovarian granulosa cells after culturing for 24 hours, and continue culturing for 2 days at a temperature of 37°C, the cultivation humidity is saturated humidity, and the extern...

Embodiment 3

[0044]A method for promoting in vitro maturation of human immature oocytes using 3D printing technology, the method comprising the following steps:

[0045] (1) Select ovarian cells with a diameter between 50 and 60 μm, wash them with normal saline, and collect naked eggs and granulosa cells digested with digestive enzymes to form ovarian cells;

[0046] (2) Put the in vitro maturation culture medium in the culture dish, then put the ovarian granulosa cells, cover with mineral oil, and put them in a CO2 incubator for balanced culture for 24 hours. The culture temperature is 37°C, the culture humidity is saturated humidity, and the outside air The environment contains 5.0% CO2;

[0047] (3) Pick out the apoptotic oocytes, with each droplet containing 15 oocytes as the standard, put the droplet into the ovarian granulosa cells after culturing for 24 hours, and continue culturing for 2 days at a temperature of 37°C, the cultivation humidity is saturated humidity, and the externa...

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Abstract

The invention relates to the technical field of biological 3D printing, in particular to a method for facilitating in-vitro maturation of human immature oocytes by utilizing a 3D printing technology. The method comprises the following steps of (1) properly treating naked ova and granular cells of ovary cells; (2) performing in-vitro balanced culture of ovarian granular cells; (3) putting oocytes into the ovarian granular cells, and continuing to perform culture for 2 days; (4) preparing 3D printing ink; (5) preparing 3D printing gel; and (6) printing out follicles by adopting the 3D printing technology, putting the oocytes and an in-vitro maturation culture solution into the follicles, and performing culture for one week. According to the method, the follicles with a biochemical environment similar to that of a human body try to be simulated, and then the oocytes are quickly and accurately put into the follicles for performing artificial culture by depending on the 3D printing technology, so that the in-vitro maturation of the human immature oocytes is realized; the method depends on the 3D printing technology; and the whole process is quick and convenient, and the automation degree is high, so that the technical expense of the in-vitro maturation of the oocytes can be reduced.

Description

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Claims

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Application Information

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Owner GUANGXI MEDICAL UNIVERSITY
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