Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Construction of a protein tag vector and its expression and detection method

A construction method and protein technology, applied in the field of biomarkers, can solve problems such as difficulty, non-expression, and uncertainty of the length of the target gene promoter

Active Publication Date: 2021-11-05
ANHUI NORMAL UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are the following problems: First, the effective promoter length of the target gene is uncertain
If the promoter is too short, it may not be effective, and the GFP fusion protein will not be expressed, and if it is too long, it will be difficult to amplify

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Construction of a protein tag vector and its expression and detection method
  • Construction of a protein tag vector and its expression and detection method
  • Construction of a protein tag vector and its expression and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0038] It should be noted that the following biotechnology terms are involved in the embodiments of the present invention:

[0039] (1) Marking of the last exon: firstly, the penultimate exon of a gene in the genome (here, the nanos gene, called the target gene) (here, the third exon of the nanos gene, Exon 3) downstream partial sequence", "the last intron (here is the 3rd intron of the nanos gene, Intron 3)", "the last exon (here is the 4th exon of the nanos gene , Exon 4) and its downstream 100bp sequence" were amplified and cloned, called the target sequence. Then, a tag sequence (such as GFP) is inserted in front of the stop codon (such as TAG) after the internal coding sequence of the exon at the end of the target sequence to obtain the transformed target sequence. Finally, the modified target sequence is integrated into the genome through the carrier of the transgene, and the sequence can participate in the RNA splicing and protein translation of the target gene in the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the construction of a protein label carrier and its expression and detection methods, and relates to the technical field of biomarkers. Taking the nos gene highly expressed specifically in the ovarian germ cells of Drosophila as an example, the invention develops a cell endogenous The protein labeling technology, that is, the protein labeling technology based on the last exon labeling; firstly, with the help of molecular cloning technology, the last exon of the nos gene was constructed with the GFP tag sequence transgenic vector; secondly, with the help of Drosophila maturation Drosophila carrying this transgenic vector was prepared by the transgenic technology, and by detecting the expression of nos gene expression product GFP fluorescence in the ovarian cells, this technology provides convenience for the study of the expression pattern of the model organism Drosophila gene, and provides a basis for mammals It provides a reference for the study of gene expression and localization.

Description

technical field [0001] The invention relates to the technical field of biomarkers, in particular to the construction of a protein tag carrier and its expression and detection methods. Background technique [0002] The process of RNA splicing is the process of excising non-coding regions called introns from mRNA precursor molecules and splicing the coding regions of genes called exons to form mature mRNA. It is a very important biological process in the gene expression of eukaryotic cells. Through RNA splicing, many functional mRNAs with coding information can be produced. It is very important for the development and evolution of organisms. The RNA splicing body is responsible for the RNA splicing process. Specifically, it cuts the RNA at the junction of the exon and the intron of the initial transcription product of the mRNA (Precursor mRNA, referred to as pre-mRNA), and then connects the exons in turn, cutting out Introns make up a complete mRNA. Studies have shown that t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/65C12N15/85A01K67/033G01N33/68
CPCA01K67/0339A01K2227/706A01K2267/03C12N15/65C12N15/85G01N33/68G01N2333/43595
Inventor 陈冬生陶小倩孙铭钟吴华勤
Owner ANHUI NORMAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com