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Biomarker for diagnosing ovarian cell apoptosis and injury caused by heavy metal uranium

A technology of biomarkers and ovarian cells, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., and can solve problems such as malignant diseases of metal uranium

Pending Publication Date: 2021-04-13
CHINA INST FOR RADIATION PROTECTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, while widely used, the high toxicity of metal uranium causes malignant diseases and a large number of uncomfortable symptoms due to its unknown etiological factors

Method used

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  • Biomarker for diagnosing ovarian cell apoptosis and injury caused by heavy metal uranium
  • Biomarker for diagnosing ovarian cell apoptosis and injury caused by heavy metal uranium
  • Biomarker for diagnosing ovarian cell apoptosis and injury caused by heavy metal uranium

Examples

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Embodiment 1

[0021] 1. Cell culture and infection

[0022] Hamster ovary cells (CHO-K1) adherent growth at 25cm 2 Add 90% F-12K culture medium, 10% FBS fetal bovine serum, 1% penicillin and streptomycin F-12K complete culture solution 5ml in the culture flask, in 5% CO 2 , and incubate in a 37°C cell culture incubator. Vaccination 5*10 4 The cells are kept in culture flasks, the cell state is observed every day, and the cells are subcultured every 2-3 days to maintain the viability of the cells. The doubling time of CHO-K1 cells in the exponential growth phase is about 8-12 hours, and the adherent growth efficiency is high.

[0023] The exposure solution is uranyl nitrate with a concentration of 500 μmol / L. Use CO 2 Incubate at 37°C in an incubator for 24 hours. Reagents were used to detect the survival rate of the cells in the control group and the experimental group, and the changes in the apoptosis cycle of the cells in the control group and the experimental group were detected by...

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Abstract

The invention belongs to the technical field of biomarkers, and relates to an application of a biomarker to preparation of a reagent / reagent group for diagnosing ovarian cell apoptosis and injury caused by heavy metal uranium. The biomarker is selected from one, more or all of the following genes: Casp3, Fos, Tgfb2, Fgf17, Faslg, Dusp2, Fgf9, Cacng5, Relb, Gadd45b, Hspa1a, Fgf7, Rap1a, Pla2g4c, Cacna1i, Cacna1b, Myc, Pla2g4f, Hspb1, Jun, Cacna1c, Ddit3, Dusp8, Cacnb4, Cacna1f, Rasgrp2, Map4k2, Nr4a1, Rac3, Fgfr4, Map4k1, Cacna1d, Fgf8, Mapk11 and Cd14. By utilizing the application of the biomarker to preparation of the reagent / reagent group for diagnosing ovarian cell apoptosis and injury caused by heavy metal uranium, the biomarker for diagnosing ovarian cell apoptosis and injury caused by heavy metal uranium can be screened out, and analysis of biological functions and pathways influenced by the biomarkers is performed.

Description

technical field [0001] The invention belongs to the technical field of biomarkers, and relates to the use of biomarkers in the preparation of reagents / reagent groups for the diagnosis of ovarian cell apoptosis damage caused by heavy metal uranium. Background technique [0002] Metal uranium has unique characteristics, including high density, high strength, high hardness, good toughness, easy spontaneous combustion, easy extraction, and low price. Metal uranium is widely used as the outer cladding material of fast breeder reactor; the material of balance weight of aircraft and forklift; the keel of yacht; the catalyst for the synthesis of acrylonitrile from propylene and ammonia; high-performance screw rotor, inertial flywheel; iron and steel smelting and ceramics Industrial glaze additives, etc. [0003] But while it is widely used, the high toxicity of metal uranium leads to malignant diseases and a large number of uncomfortable symptoms due to its unknown etiological fact...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156Y02A50/30
Inventor 李建国高洁尹晶晶潘泽轩秦秀军刘欢
Owner CHINA INST FOR RADIATION PROTECTION
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