45 results about "Blood corpuscles" patented technology

The invention belongs to agents for medical diagnostic apparatuses, and in particular relates to an agent for a blood cell analyzer. The agent for the blood cell analyzer consists of a diluent, an EOI hemolytic agent, an LH hemolytic agent, an EOII staining solution and a cleaning solution. The agent for the blood cell analyzer is wide in range of use, suitable for many types of machines, suitable for detectors with machine types of BC5100 / 5180 / 5300 / 5380 / 5310 / 5390 and the like, and stable and accurate in detection result; the R value of a correlation coefficient after linear regression reaches more than or equal to 0.99 compared with a Mairui original blood cell agent and expresses relatively good relativity; and moreover, the agent is rich in raw material and low in price, and the raw materials are nontoxic and harmless and do not have threats on operators, so that the burden of the environment is lightened, and the raw materials are beneficial for popularization and application of the agent for the blood cell analyzer.

A corpuscle / plasma separating part is disposed at the lower end of the substrate, and a sensor part connected to the corpuscle / plasma separating part is disposed at the upper end of the substrate, with a calibration solution reservoir being disposed on the lower side of the sensor part, and a calibration solution waste reservoir being disposed on the upper side of the sensor part. A first centrifugal axis is located upper to the corpuscle fraction storing part and lower to the plasma fraction storing part of the corpuscle / plasma separating part, while a second centrifugal axis is located within or close to the sensor part. Conveyance and discharge of the calibration solution can be carried out by performing centrifugation around the first centrifugal axis which is distant from the sensor part at a low speed of rotation, so that the centrifugal force exerted on the sensors would be small. During the centrifuge operation at a high speed of rotation for the separation of blood corpuscles, centrifugation can be performed around the second centrifugal axis so that the centrifugal force exerted on the sensors is small. Centrifuge operation allows separation of the blood corpuscles and blood plasma, and conveyance of the blood plasma and the calibration solution, as Well as certain discharge of the calibration solution from the sensors, thereby allowing precise analysis. Any damage in the sensors due to strong centrifugal force during the separation of blood corpuscles and blood plasma can be prevented.

The invention relates to a swine hemoglobin enzymolysis and decoloration technology. The technology comprises the following steps of: performing centrifugal separation on swine blood to form blood corpuscles, adding water and quickly stirring; regulating pH value, and heating for boiling; adding a composite protease preparation, and performing enzymolysis at certain temperatures; performing enzyme inactivation after the enzymolysis, filtering, and performing spray drying on supernate to obtain gray white hemoglobin polypeptide; and drying precipitates to obtain a heme coarse product. By the technical scheme provided by the invention, hemoglobin polypeptide powder and the heme coarse product are obtained, wherein the hemoglobin polypeptide is a good animal protein source and is used as a feed additive to improve the digestion and absorption rates of protein; heme can be used as a chromogenic reagent in food industry and can be used as a pharmaceutical raw material in medical industry; and the hemoglobin polypeptide and the heme are products with high added values. The technical scheme has important significance of improving processing value-added capability of animal blood products, broadening comprehensive utilization ways, and promoting the technology upgrading of the whole industry.

The invention provides benzpyrole squaric acid cyanine dye. The general formula of the structure of the benzpyrole squaric acid cyanine dye is shown in the description, wherein X is one of Br, Cl and I, and R is one of the following structures shown in the description. The benzpyrole squaric acid cyanine dye provided by the invention has good stability and large absorption strength, can be applied in matching reagents of a 5-classified hematology analyzer to be used as dye of nucleated red blood cells, and can also be applied to fluorescent marks and other life science research fields.

The invention discloses a blood plasma albumen and / or blood corpuscle albumen preparing method, which adopts a high pressure homogenizing machine to dispose the fresh animal blood plasma and / or blood corpuscle under the pressure of 60 to 120 MPa and mechanically sterilize the pathogen microbe and lead to the fresh blood corpuscle cell membrane breaking. The generated blood plasma albumen powder and / or blood corpuscle albumen powder have high security and greatly improve the digestion and absorption rate.

The invention relates to a preparation method of a direct antihuman globulin reagent card. The preparation method is characterized in that the reagent card comprises six microcolumn gel tubes respectively comprising two gel tubes containing anti-IgG, two gel tubes containing anti-C3d and two gel tubes containing gel suspension mediums, and each reagent card is shared by two persons. The method comprises the technical processes of preparation of the gel suspension mediums, preparation of gel, selection of antibody as well as preparation and split charging of gel. The direct antihuman globulin reagent card prepared by the method has high sensitivity, good specificity and stable quality, red blood corpuscles having sensitization to IgG antibodies and the red blood corpuscles having sensitization to C3d alexin can be detected by using the same card at the same time, and the red blood corpuscles having sensitization to IgG antibodies or the C3d alexin can be accurately determined.

The invention discloses an impedance method detection system of a blood cell analyzer and a method for identifying hole blockage of an impedance channel. The method for identifying hole blockage of the impedance channel comprises the steps: acquiring historical average pulse data, and calculating a mean value and a standard deviation of the historical average pulse data; acquiring voltage pulse data of the sampling analysis, and counting average pulses of cells passing through small holes in each unit time; sequentially calculating the front mean value and the rear mean value of each unit time, and judging the unit time of possible hole blockage according to the change conditions of the front mean value and the rear mean value of each unit time; and judging whether hole blockage occurs in unit time of possible hole blockage for the first time, comparing the second variable quantity with a standard deviation of historical average pulse data after judging that hole blockage does not occur in unit time of possible hole blockage for the first time, and judging whether hole blockage occurs in unit time of possible hole blockage for the second time. The identification and report of the whole-process hole blockage condition are realized.

The invention discloses a multidirectional valve hydrocephalus shunt valve with antibacterial and anticoagulant functions, and preparation and an application method thereof, and belongs to the field of medical equipment. Two ends of a drainage tube are sealed through a silicone hose; a plurality of long seams are axially cut on the side wall at one end of the drainage tube, so as to form a valve type channel; and a hole is punched in the side wall at the other end of the drainage tube, which is served as a channel for allowing intracranial liquid to flow into the hydrocephalus shunt. At least one type of antibacterial drug permeates in the shunt valve body, so as to prevent wound from being infected; and meanwhile, anticoagulant drugs are connected with the surface of the tube wall in a surface grafting manner, so as to prevent blood corpuscle and protein in hydrocephalus from being adhered to the inner tube wall and causing lumen blockage. The shunt valve is used for the drainage of hydrocephalus from brain to other body cavities, the antibacterial and anticoagulant design can effectively prevent infection and vessel clogging in hydrocephalus shunt, the multidirectional valve design at the remote end can prevent liquid from flowing back, and the hydrocephalus shunt valve is multifunctional.

The invention relates to a detection kit, specifically to a kit for detecting alpha-1,3Gal antigen and application thereof. According to the invention, a solid phase antigen coated plate is prepared from Gal1,3Gal-BSA antigen in advance, and then the antibody competitive ELISA inhibition method detection kit is prepared through combination with the specific antibody M86 of the alpha-1,3Gal antigen. The kit provided by the invention is applicable to qualitative and quantitative detection of alpha-Gal antigen of a variety of biomaterials, especially to detection of residual alpha-Gal in animal-derived biomaterials. The specificity of the kit reaches 100%, and the sensitivity of the kit is embodied by capacity of detection of rabbit blood corpuscles with a concentration of 3.9 * 10<4> / ml and Gal antigen in a Gal antigen positive reference with a concentration of 6.25 mu g / ml.

The invention relates to a self-adjusting rotor used for conveying blood or conveying shear sensitive fluid. The rotor rotates in a pump body so that the pressure of the fluid is increased and the fluid flows out along the circumferential direction relative to the flowing direction of a medium, the flowing direction comprises an incident flow face (3) and a back flow face (4), and an upper end face and a lower end face of the rotor are an upper oblique plane and a lower oblique plane; if the rotor is cut along a central axis, an included angle formed by an intersecting line of the upper oblique plane and a section and a rotary horizontal plane is an upper top angle beta, and the beta is more than 0 degree; an included angle formed by an intersecting line of the lower oblique plane and the section and the rotary horizontal plane is a lower bottom angle alpha, and the alpha is more than or equal to 0 degree; the number of the rotors is even, and the rotors are symmetrically distributed along a rotating shaft; and rotor clearances are formed between the upper end face and the lower end face of the rotor and the pump body, the rotor clearances are the distances from each point on the upper end face and the lower end face to the inner surface of the corresponding pump body, and the rotor clearances from the incident flow face to the back flow face are reduced progressively. The self-adjusting rotor has extremely high self-balancing and self-adjusting properties. When the self-adjusting rotor is applied to an artificial heart, the rotor can reduce the damage to blood corpuscles, improve the compatibility with the blood, and lower the manufacturing cost.

The invention discloses a cell separating, preparation and dyeing integrated device and a circulating tumor cell capturing method. A chip comprises a base material and a channel which extends into the base material, wherein a sample inlet and a sample outlet are respectively formed in two ends of the channel; n rows of micro-column arrays are arranged in the channel in an arrayed manner, and n is greater than or equal to 2; each row of micro-column arrays respectively comprises a plurality of micro-columns which are arranged at intervals; a capturing gap is formed between each two adjacent micro-columns, and the following conditions are met: (a), the capturing gaps of an xth row of micro-column arrays are greater than the capturing gaps of a (x+1)th row of micro-column arrays from the sample inlet to the sample outlet, and x is greater than or equal to 1 and smaller than n; and (b), the capturing gaps of the nth row of micro-column arrays are smaller than the outer diameters of circulating tumor cells. The method has the advantages of small amount of consumed samples, high resolution precision and sensitivity, easiness in integration and miniaturization and the like. In addition, the circulating tumor cells are captured on the basis of the characteristic that the boundary dimension of the circulating tumor cells is different from that of blood corpuscles, and capturing of the circulating tumor cells does not depend on expression of an EpCAM antibody.

The invention relates to a new process of a technology for extracting superoxide dismutase from animal blood. The new process is characterized by comprising the following steps of: (1) separation of blood cells; (2) removal of hemoglobin; (3) thermal denaturation: heating supernatant to be 65 DEG C, insulating for 10 minutes, then quickly cooling to room temperature, centrifuging for 20 minutes at a rotating speed of 3000r / min, removing precipitates and collecting the supernatant; (4) precipitation: cooling the supernatant in salt-ice bath, then adding 1.5 times of precooling acetone at the operating temperature below minus 5 DEG C while stirring uniformly and then generating white precipitate; and (5) purification of SOD (Superoxide Dismutase): adopting a method for combining thermal denaturation, isoelectric point and acetone fractional precipitation, firstly treating a SOD crude extract solution for 15 minutes at the temperature of 55 DEG C, then adjusting the pH value till the isoelectric point is 3-4, finally adding the acetone with the final volume of being 1.0%, and collecting the precipitates; adding deionized water into the precipitate to obtain a solution which is an SOD purified liquid; and carrying out purification by adopting a DEAE Sepha-dexA-50 chromatographic column to obtain a purified product which is an SOD fine liquid.

The invention discloses a novel process for extracting superoxide dismutase SOD from animal blood cell liquid. The process mainly comprises the following steps of: after carrying out anticoagulation treatment on animal fresh blood, centrifuging and separating blood cells; carrying out hemolysis, thermally denaturing, super-filtering and concentrating, and then carrying out fractional precipitation by acetone to obtain a rough product of the SOD; subjecting the rough product of the SOD to chromatographic purification, and then freezing and drying to obtain a competitive product of the SOD. In the process provided by the invention, chloroform ethanol is abandoned and transitional metal salt which is any one of copper salt, zinc salt and manganese salt in the process of thermally denaturing is not added; a ultra-filtering and concentrating process is further introduced on the base and the use amount of the acetone in the process of carrying out the fractional precipitation by acetone is extremely small. The novel process for extracting the SOD guarantees the activity of the traditional SOD to the maximum extent, has the advantages of simplicity in operation and low cost and is suitable for large-scale industrial production.

The invention discloses a compact multifunctional medical square cabin. The medical square cabin (1) comprises a square cabin body (2) and medical diagnosis equipment, wherein the medical diagnosis equipment comprises a digital camera shooting system, a nuclear magnetic resonance system and physical examination complete equipment; the digital camera shooting system comprises a double-stand-columndigital camera shooting instrument (8), a high pressure generator (9) and a power distribution cabinet (10); the nuclear magnetic resonance system comprises a nuclear magnetic resonance cabinet (11),an isolation transformer (12), a nuclear magnetic resonance magnet (13) and a nuclear magnetic resonance scanning bed (14); the physical examination complete equipment comprises a B supersonic diagnostic set (15), an electrocardiograph (16), a biochemical analyzer (17), a urea examination machine (18) and a blood-cell counter (19); and a nuclear magnetic resonance equipment room (3), a nuclear magnetic resonance scanning room (4), an integrated physical examination operation room (5), a digital camera shooting scanning room (6) and a box equipment room (7) are successively arranged in the medical square cabinet. The compact multifunctional medical square cabin is used, involved diagnosis functions are multiple, and costs are low.

The present invention relates to specific component in a blood specimen containing a blood corpuscle, such as consistency measurement technology of glucose. In the present invention, to calculate the consistency of the specific component in consideration of the diffusion speed of the specific component diffused to the outside of the blood corpuscle from the inside thereof. Under ideal condition, the diffusion speed is showed as a Michaelis-Menten equation: V = V [max] delta S / ( K [m] + delta S), wherein V [max] is potential maximum film permeance speed of the specific component from the blood corpuscle to outside, K [m] is michalis constant (the value of delta S when V = V [ max ] / 2), delta S is the consistency difference between the inner and outer of the specific components of the blood corpuscle.

The invention discloses a small hole blockage identification method and device, and the method comprises the steps: carrying out the derivation of a small hole voltage curve, obtaining an inflection point of the small hole voltage curve, solving the integral of a derivative function between the inflection points to identify the blockage condition, and obtaining a blockage occurrence or ending timepoint through combining with the position of the inflection point. When hole blockage is found, the small hole blockage working condition can be further judged in combination with the average voltageof the small hole voltage curve within the preset time period, small hole blockage is prevented from being not reported or misreported, and therefore the small hole blockage working condition of thesmall hole can be recognized more accurately.

The invention discloses an optical system of a blood cell analyzer, an optical gain calibration method and a storage medium, wherein the method comprises the steps: obtaining a current first gain, and calculating a first calibration parameter of each angle; judging whether an optical system is normal or not according to the first calibration parameters of the three angles, and adjusting the first gain to the second gain if the optical system is judged to be normal; calculating a second calibration parameter of each angle, judging whether the optical system is normal or not according to the second calibration parameters of the three angles, and calculating the calibration gain of each angle according to the first calibration parameter and the second calibration parameter of the three angles if the optical system is judged to be normal; judging whether the calibration gain at each angle is within a threshold range or not, and storing the calibration gain if the calibration gain at each angle is within the threshold range. and calculating the calibration gain according to the first calibration parameters and the second calibration parameters, so as to reducing the requirement of optical gain calibration on the consistency of the instrument.

A method of measuring hematocrit and a method of testing blood are provided. The method for measuring hematocrit includes the following steps. A test strip is provided. The test strip includes a reaction region and a pair of electrodes disposed in the reaction region. A whole blood sample is entered to the reaction region. After the whole blood sample enters the reaction region, a set of square wave voltages is applied to the pair of electrodes based on a square wave voltammetry method to obtain a feedback related to hematocrit. A difference between an initial time when the whole blood sampleenters the reaction region and an initial time when the set of square wave voltage is applied ranges from 0.1 seconds to 200 seconds. A hematocrit value is calculated according to the feedback.

The invention discloses a whole blood quality control material. The whole blood quality control material comprises three cell simulating materials, an anticoagulation agent, white cell stationary liquid, and red cell and blood platelet stationary liquid. The invention further discloses a preparation method of the whole blood quality control material. The whole blood quality control material is wide in raw material resources and simple in preparation process; three classes of white cells can be clearly identified on a three-class blood cell instrument; the size difference of red cells and blood platelets is obvious, and the red cells and the blood platelets are easy to identify by the instrument; interferences of the small red cells on the blood platelets are small and the storage life can reach 6 months; the use requirements of clinical quality control are met.

The invention relates to a preparation method of a direct antihuman globulin reagent card. The preparation method is characterized in that the reagent card comprises six microcolumn gel tubes respectively comprising two gel tubes containing anti-IgG, two gel tubes containing anti-C3d and two gel tubes containing gel suspension mediums, and each reagent card is shared by two persons. The method comprises the technical processes of preparation of the gel suspension mediums, preparation of gel, selection of antibody as well as preparation and split charging of gel. The direct antihuman globulin reagent card prepared by the method has high sensitivity, good specificity and stable quality, red blood corpuscles having sensitization to IgG antibodies and the red blood corpuscles having sensitization to C3d alexin can be detected by using the same card at the same time, and the red blood corpuscles having sensitization to IgG antibodies or the C3d alexin can be accurately determined.

The invention relates to a new process of a technology for extracting superoxide dismutase from animal blood. The new process is characterized by comprising the following steps of: (1) separation of blood cells; (2) removal of hemoglobin; (3) thermal denaturation: heating supernatant to be 65 DEG C, insulating for 10 minutes, then quickly cooling to room temperature, centrifuging for 20 minutes at a rotating speed of 3000r / min, removing precipitates and collecting the supernatant; (4) precipitation: cooling the supernatant in salt-ice bath, then adding 1.5 times of precooling acetone at the operating temperature below minus 5 DEG C while stirring uniformly and then generating white precipitate; and (5) purification of SOD (Superoxide Dismutase): adopting a method for combining thermal denaturation, isoelectric point and acetone fractional precipitation, firstly treating a SOD crude extract solution for 15 minutes at the temperature of 55 DEG C, then adjusting the pH value till the isoelectric point is 3-4, finally adding the acetone with the final volume of being 1.0%, and collecting the precipitates; adding deionized water into the precipitate to obtain a solution which is an SOD purified liquid; and carrying out purification by adopting a DEAE Sepha-dexA-50 chromatographic column to obtain a purified product which is an SOD fine liquid.

The invention discloses a preparation method for decolorized blood globulin powder. The method comprises the following steps: sampling fresh blood of a health animal; adding an anticoagulant to carry out anticoagulation, and separating to obtain a blood globulin liquid; adding a salt solution with the concentration of 0.3-0.6% to the blood globulin liquid, wherein the amount of the salt solution is 1-4 times the amount of the blood globulin liquid; carrying out high speed stirring for 1-2 hours, and heating to a temperature of 60-70 DEG C by water bath; adding an antifoaming agent to the heated blood globulin liquid, wherein the volume of the antifoaming agent is 1.0-5.0% of the volume of the blood globulin liquid; immediately adding a calcium peroxide solution with the concentration of 0.5-1.5 g / ml, and completely stirring, wherein the volume of the calcium peroxide solution is 2.0-6.0% of the volume of the blood globulin liquid; adopting hydrochloric acid to adjust the pH value to 3.5-5.5, and adopting a 10% sodium hydroxide solution to adjust the pH value to 6.5-7.5; carrying out spray drying to obtain the decolorized blood globulin powder. The method of the present invention has characteristics of simple operation and low cost, and is applicable for the large scale industrial production.

The invention discloses a package analyzer for detecting blood transfusion compatibility. The package analyzer comprises a serum blood cell sample injector, a test tube combiner and a manipulator foroperating a sample injector to add a sample into a test tube combiner, and further comprises a polyamine adding device and a deflocculating agent adding device. The manipulator can operate the polycondensation amine adding device to add a polycondensation amine reagent into the test tube combiner, the polyamine adding device comprises a fixed frame connected with the manipulator, and a reagent bottle and two injectors are arranged in the fixed frame. A bypass pipe communicated with the bottom of the reagent bottle is arranged at the bottom of each of the two injector needle cylinders, the topsof the push rods of the two injectors are each provided with a sliding support extending downwards, and the two sliding supports are oppositely arranged. The opposite sides of the two sliding supports are each provided with a rack, a servo motor is further installed in the fixing frame, a gear is installed at the output end of the servo motor, and the two sides of the gear are meshed with the tworacks at the same time. The package analyzer enables the operation efficiency of the blood transfusion compatibility test to be improved, and errors are not likely to happen.

The invention provides a sample analysis method and a sample analysis system, the sample analysis system comprises a first sample analyzer, a second sample analyzer and a sample conveying device, thefirst sample analyzer is a blood cell analyzer used for detecting a whole blood sample to be detected so as to obtain a blood routine examination result; the second sample analyzer is an analyzer usedfor detecting the whole blood sample to be detected to obtain a corresponding detection result, and the sample analysis method comprises the following steps: acquiring first detection data of the whole blood sample to be detected on the first sample analyzer and second detection data of the whole blood sample to be detected on the second sample analyzer; acquiring actual correction parameters corresponding to the whole blood sample to be detected according to the first detection data; obtaining an actual detection result corresponding to the whole blood sample to be detected according to thesecond detection data; and correcting the actual detection result according to the actual correction parameters. The method and the system can improve the accuracy of the detection result.

The invention provides a sample analysis method and a sample analysis system. The sample analysis system comprises a first sample analyzer and a second sample analyzer, the first sample analyzer is ablood cell analyzer used for detecting a whole blood sample to be detected to obtain a blood routine examination result; the second sample analyzer is an analyzer for detecting the whole blood sampleto be detected to obtain a detection result; the sample analysis method comprises the following steps: acquiring first detection data of the whole blood sample to be detected on the first sample analyzer; acquiring actual correction parameters of the whole blood sample to be detected according to the first detection data; acquiring a default detection result of the whole blood sample to be detected on the second sample analyzer, wherein the default detection result is a detection result corrected according to the default correction parameter; acquiring an actual detection result of the whole blood sample to be detected on the second sample analyzer according to the default detection result and the default correction parameter; and correcting the actual detection result according to the actual correction parameter. The method and the system can improve the accuracy of the detection result.