Cell separating, preparation and dyeing integrated device and circulating tumor cell capturing method

Abstract

The invention discloses a cell separating, preparation and dyeing integrated device and a circulating tumor cell capturing method. A chip comprises a base material and a channel which extends into the base material, wherein a sample inlet and a sample outlet are respectively formed in two ends of the channel; n rows of micro-column arrays are arranged in the channel in an arrayed manner, and n is greater than or equal to 2; each row of micro-column arrays respectively comprises a plurality of micro-columns which are arranged at intervals; a capturing gap is formed between each two adjacent micro-columns, and the following conditions are met: (a), the capturing gaps of an xth row of micro-column arrays are greater than the capturing gaps of a (x+1)th row of micro-column arrays from the sample inlet to the sample outlet, and x is greater than or equal to 1 and smaller than n; and (b), the capturing gaps of the nth row of micro-column arrays are smaller than the outer diameters of circulating tumor cells. The method has the advantages of small amount of consumed samples, high resolution precision and sensitivity, easiness in integration and miniaturization and the like. In addition, the circulating tumor cells are captured on the basis of the characteristic that the boundary dimension of the circulating tumor cells is different from that of blood corpuscles, and capturing of the circulating tumor cells does not depend on expression of an EpCAM antibody.

Description

technical field [0001] The application belongs to the field of in vitro diagnosis and microfluidic technology, and in particular relates to an integrated device for cell separation, preparation and staining and a method for capturing circulating tumor cells. Background technique [0002] Circulating tumor cells (CTCs) refer to tumor cells that have diffused from the primary tumor into the peripheral blood circulation system, and their appearance indicates that tumor cells have begun to spread from the lesion to the surroundings, which may lead to more organs and tissues. produce lesions. [0003] Circulating tumor cells can appear in the early stage of tumorigenesis, and studies have shown that about 90% of cancer death cases are related to the spread of CTCs. Therefore, CTCs detection is important in the early prevention of major diseases, evaluation of treatment effects, drug sensitivity testing, and tumor recurrence monitoring. It is of great significance in medical appl...

AI Technical Summary

Problems solved by technology

Although the counting has high sensitivity, there are expensive instruments and reagents consumed, and the limitation of using antibodies to capture cells is large. Not all tumor cells express EpCAM, and the detection efficiency is low.

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