An integrated device for poration of
biological tissue, harvesting a
biological fluid from the tissue, and analysis of the
biological fluid. The device comprises a tissue-contacting layer having an electrically or optically heated probe to heat and conduct heat to the tissue to form at least one opening, such as a micropore to collect
biological fluid from the opening, and a detecting layer responsive to the biological fluid to provide an indication of a characteristic of the biological fluid, such as the concentration of an
analyte in
interstitial fluid. In the embodiment in which, the probe comprises a photosensitizing
assembly designed for the uniform application of a photosensitizing material, such as, for example, a dye or a
pigment, to a tissue, e.g., the stratum comeum. In one embodiment, the photosensitizing
assembly comprises photosensitizing material combined with a carrier, such as, for example, an
adhesive or an ink, and the resulting combination is applied to a substrate, such as, for example, an
inert polymeric substrate to form a photosensitizing
assembly. In another embodiment, the photosensitizing assembly comprises photosensitizing material incorporated into a film-forming polymeric material.