A method for the detection of an early biomarker for assessing a change in renal status in a mammalian subject following a renal event. The method typically includes the steps of (a) providing a
body fluid sample obtained from a mammalian subject; (b) analyzing the molecular weight of the proteins in the sample using
proteome analysis; and (c) identifying the presence of a
protein in the sample selected from the group consisting of a 6.4 kDa
protein, a 28.5 kDa
protein, a 33 kDa protein, a 44 kDa protein, a 67 kDa protein, and combinations thereof. The presence of one of these proteins can serve as an early biomarker for assessing a change in renal status. The levels of these proteins can be compared to predetermined levels, and thus provide a determination of the subject's renal status. The invention also includes a method of assessing the administration of
aprotinin during cardio-pulmonary
bypass surgery and provides for methods where the level of the 6.4 kDa biomarker in the subject's
urine directs a caregiver's therapeutic decision regarding the intra-operative administration of
aprotinin.