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New process of technology for extracting superoxide dismutase from animal blood

A technology of superoxide and dismutase, applied in the direction of oxidoreductase, etc., can solve the problems of high cost, complex SOD process, and many production equipment, and achieve the effect of simple operation, convenient and fast operation, and simple method

Inactive Publication Date: 2013-11-20
ANHUI XIAOGANG BAODI PIG TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The content of the present invention is to overcome the shortcomings of conventional methods for extracting SOD in industrialized production, such as complex process, many production equipments, and high cost, and propose a new process that is simple and convenient in process, low in cost, easy to operate, and suitable for industrialized production

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0009] 2, Example 1: A new process for extracting superoxide dismutase from animal blood, characterized in that the process comprises the following steps, (1) separating blood cells, taking fresh animal blood, adding it to 3.8% trisodium citrate anticoagulant solution, and freshly The ratio of animal blood to anticoagulant solution is 3:1, stir gently, centrifuge at 4000r / min for 20min, and collect red blood cells; Repeat three times, then add 1 to 1.1 times the volume of deionized water to the washed red blood cells, stir the hemolysis for 30 minutes, then slowly add 0.25 times the volume of pre-cooled 95% ethanol and 0.15 times the volume of pre-cooled chloroform to the hemolysis, and vigorously Stir for about 15 minutes, let stand for 1 hour, then centrifuge at 4 000 r / min for 20 minutes to remove the denatured hemoglobin precipitate, take the supernatant, filter, and collect the filtrate; (3) heat denaturation, heat the supernatant to 65°C, keep it warm for 10 minutes, and...

Embodiment 2

[0010] Example 2: As an improvement of the present invention: when performing anticoagulant treatment in the step (1), the sodium citrate used is added in an amount of 2.0-3.0 g / Kg animal blood, and the stirring hemolysis time is 20-30 min. All the other steps are identical to Example 1.

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PUM

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Abstract

The invention relates to a new process of a technology for extracting superoxide dismutase from animal blood. The new process is characterized by comprising the following steps of: (1) separation of blood cells; (2) removal of hemoglobin; (3) thermal denaturation: heating supernatant to be 65 DEG C, insulating for 10 minutes, then quickly cooling to room temperature, centrifuging for 20 minutes at a rotating speed of 3000r / min, removing precipitates and collecting the supernatant; (4) precipitation: cooling the supernatant in salt-ice bath, then adding 1.5 times of precooling acetone at the operating temperature below minus 5 DEG C while stirring uniformly and then generating white precipitate; and (5) purification of SOD (Superoxide Dismutase): adopting a method for combining thermal denaturation, isoelectric point and acetone fractional precipitation, firstly treating a SOD crude extract solution for 15 minutes at the temperature of 55 DEG C, then adjusting the pH value till the isoelectric point is 3-4, finally adding the acetone with the final volume of being 1.0%, and collecting the precipitates; adding deionized water into the precipitate to obtain a solution which is an SOD purified liquid; and carrying out purification by adopting a DEAE Sepha-dexA-50 chromatographic column to obtain a purified product which is an SOD fine liquid.

Description

technical field [0001] The invention relates to a process for extracting superoxide dismutase (SOD), in particular to a new process for extracting superoxide dismutase (SOD) from animal blood cells. [0002] Background technique [0003] SOD is an active substance derived from living organisms. It is composed of protein and metal ions. It is the most important key metalloenzyme for body cells to resist oxidative damage. It has anti-oxidation, anti-aging, anti-fatigue, anti-tumor, anti-radiation, and improved immune functions. , reduce blood pressure and blood lipids, prevent and assist diabetes, prevent prostatitis, prevent chronic diseases and their complications, eliminate side effects of chemotherapy, avoid secondary injuries of surgery, prevent and treat skin diseases, and accelerate wound healing. SOD has multiple functions such as anti-aging, anti-tumor, anti-radiation, and enhancing human immunity. It is now widely used in medicine, food, health care products, cosmet...

Claims

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Application Information

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IPC IPC(8): C12N9/02
Inventor 龙峥于福满王小乔姜无边
Owner ANHUI XIAOGANG BAODI PIG TECH
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