Optical system of blood cell analyzer, optical gain calibration method and storage medium

Abstract

The invention discloses an optical system of a blood cell analyzer, an optical gain calibration method and a storage medium, wherein the method comprises the steps: obtaining a current first gain, and calculating a first calibration parameter of each angle; judging whether an optical system is normal or not according to the first calibration parameters of the three angles, and adjusting the first gain to the second gain if the optical system is judged to be normal; calculating a second calibration parameter of each angle, judging whether the optical system is normal or not according to the second calibration parameters of the three angles, and calculating the calibration gain of each angle according to the first calibration parameter and the second calibration parameter of the three angles if the optical system is judged to be normal; judging whether the calibration gain at each angle is within a threshold range or not, and storing the calibration gain if the calibration gain at each angle is within the threshold range. and calculating the calibration gain according to the first calibration parameters and the second calibration parameters, so as to reducing the requirement of optical gain calibration on the consistency of the instrument.

Description

technical field [0001] The present application relates to the technical field of blood testing, in particular to an optical system, an optical gain calibration method and a storage medium of a blood cell analyzer. Background technique [0002] When using a blood cell analyzer to analyze a blood sample, a certain amount of blood sample is sucked in, and after it is treated with a reagent, the blood sample is injected into a conical flow chamber filled with a diluent through a nozzle. Encased in sheath fluid, individual blood cells pass through the center of the flow chamber. The blood cells suspended in the sheath fluid pass through the laser detection area after secondary acceleration. When the blood cells pass through the laser detection area, they are irradiated by the laser beam to generate scattered light. The nature of the scattered light is related to the size of the cell, the refractive index of the cell membrane and the internal structure of the cell. [0003] In or...

AI Technical Summary

Problems solved by technology

However, the existing gain calibration method has relatively high requirements for the consistency of the instrument when performing optical gain calibration, so it needs to be improved

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