Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of p110 delta and antibody of p110 delta in specifically marking trophoblast giant cell (pTGC)

A specific technology for trophoblast giant cells, which is applied in the field of cell detection and labeling, can solve the problems of numerous operation steps, low accessibility and inconvenience of ISH, and achieve the effect of wide application range, easy identification and wide application

Active Publication Date: 2014-11-19
GUANGDONG PHARMA UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Not only that, no matter from the probe design, preparation or later experiments, ISH presents a lot of inconvenience, with many steps and takes up to three days. Although there are kits produced to improve the success rate and convenience of the experiment, compared with immuno Histochemistry (IHC) and other physical structure positioning marker methods, ISH's "friendliness" is still not high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of p110 delta and antibody of p110 delta in specifically marking trophoblast giant cell (pTGC)
  • Application of p110 delta and antibody of p110 delta in specifically marking trophoblast giant cell (pTGC)
  • Application of p110 delta and antibody of p110 delta in specifically marking trophoblast giant cell (pTGC)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Detection of pTGC by immunohistochemistry (IHC)

[0036] 1. Preparation of p110δ antibody

[0037] The p110δ antibody was prepared using the partial fragment of human p110δ, ie the amino acid sequence shown in SEQ ID NO.1 as information basis.

[0038] The p110δ antibody was purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology), trade name PI3-kinase p110δ antibody (A-8) (sc-55589), is a monoclonal mouse-derived antibody, which can target mice and Human-derived specimens were tested experimentally.

[0039] 2. Obtain mouse embryos / placenta

[0040] Mice (C57BL / 6J mice, purchased from Guangdong Medical Experimental Animal Center, production license number: SCXK (Guangdong) 2008- 0002), the mouse embryos / placenta were obtained as follows:

[0041] S1. The mice were killed by neck dislocation;

[0042] S2. Lay the mouse on its back, expose the abdominal cavity with the opening in the middle of the abdomen, turn the intestinal tissue to the out...

Embodiment 2

[0068] Example 2 Immunofluorescence (IF) detection of pTGC

[0069] 1. The preparation of p110δ antibody and the acquisition of mouse embryo / placenta are the same as in Example 1.

[0070] 2. Slice and stain the embryos obtained above, and use the method of IF to detect with p110δ antibody (primary antibody). CK7 (cytokeratin 7), a well-recognized marker of trophoblast cells, was used as a positive control.

[0071] The operation process of IF is as follows:

[0072] (1) Paraffin sections were normally dewaxed to water, washed with PBS for 5 minutes, 3 times.

[0073] (2) Antigen retrieval: 0.01M citrate buffer pH=6.0 (repair solution), heat repair in boiling water bath for 15 minutes.

[0074] (3) Block with 10% fetal bovine serum (diluted in PBS) at room temperature for 1 hour

[0075] (4) Incubate with primary antibody overnight at 4°C (antibody 1: 200-fold dilution).

[0076] (5) Wash with PBS for 5 minutes, 3 times.

[0077] (6) Secondary antibody incubation (Alex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of p110 delta and an antibody of the p110 delta in specifically marking an early trophoblast giant cell (Parietal TGC, pTGC). The p110 delta is a segment of p110 delta; an amino acid sequence of the segment is as described as SEQIDNO.1. According to a record, a p110 delta antibody is used as a detection medium for specifically marking pTGC by using conventional technical methods such as an immunohistochemical method, an immunofluorescence method or a polymerase chain reaction (PCR) method; a marker is relatively simple and convenient; the detection time is greatly shortened; the marker is easy to identify, relatively high in success rate, relatively good in user acceptance and relatively convenient to use in clinic. In addition, compared with those of the nucleic acid of hybridization in situ, the observation index is in the protein level; the organism effect condition can be reflected relatively directly; the application range is relatively wide.

Description

technical field [0001] The invention belongs to the technical field of cell detection labeling. More specifically, it relates to the application of p110δ and its antibody in specifically labeling trophoblast giant cells (pTGC). Background technique [0002] The placenta is a transitional organ for the exchange of substances between mother and child formed by the joint growth of embryonic membrane and maternal endometrium during pregnancy in mammals. For the fetus developing in the womb, the placenta has functions such as supply, metabolism, excretion, secretion and immunity. Abnormal placenta will cause fetal hypoxia, distress, malnutrition, growth retardation, mental impairment, and even fetal death. Phenomenon. [0003] Trophoblast giant cells (Parietal TGC, pTGC) are a type of multinucleated giant cells linearly arranged at the implantation site and outside the yolk sac of the parietal lobe. According to their differentiation and formation, they are divided into prima...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/532C12Q1/68
CPCC12Q1/6888C12Q2600/158G01N33/532G01N2800/368
Inventor 王丽京胡曦文李江超杨雪松章倩倩
Owner GUANGDONG PHARMA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com