Compounds with EB (Epstein-Barr) virus and Kaposi's sarcoma-associated herpesvirus (KSHV) resisting functions in Hypericum japonicum, and preparation method and application thereof

A technology of Epstein-Barr virus and herpes virus, applied in the field of medicine, can solve the problems of human side effects and incomplete cure

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some progress has been made in the clinical treatment of KSHV-related diseases, but these methods cannot be completely cured, and have certain side effects on the human body

Method used

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  • Compounds with EB (Epstein-Barr) virus and Kaposi's sarcoma-associated herpesvirus (KSHV) resisting functions in Hypericum japonicum, and preparation method and application thereof
  • Compounds with EB (Epstein-Barr) virus and Kaposi's sarcoma-associated herpesvirus (KSHV) resisting functions in Hypericum japonicum, and preparation method and application thereof
  • Compounds with EB (Epstein-Barr) virus and Kaposi's sarcoma-associated herpesvirus (KSHV) resisting functions in Hypericum japonicum, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Preparation and structural identification of compounds D. antivirin B, L. antivirin B, D. antivirin D and L. anti-KS virus C.

[0051] (1) Preparation of the compounds represented by the formula (1) from C. chinensis dextrorotary antiviral toxin B, C. chinensis L. antiviral toxin B, C. chinensis antiviral D and C. sylvestris L. antiviral toxin C

[0052] 1. Collection of Hypericum japocicum:

[0053] Digeriae collected from the Dabie Mountain area of ​​Qichun County, Hubei Province: 50kg of the whole plant was collected in October, dried in the sun, dried in vacuum, and stored in the Chinese Herbal Medicine Specimen Bank of the School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology.

[0054] The sagegrass collected from Lushan District, Lushan City, Jiangxi Province: the whole herb was collected in August, dried in the sun, dried in vacuum, and stored in the Chinese Herbal Medicine Specimen Bank of the School of Pharmacy, To...

Embodiment 2

[0065] Example 2: Inhibitory activity of compound 1-10 on DNA replication of Epstein-Barr virus; inhibitory activity of compound 1-18 on lytic replication of KSHV.

[0066] Evaluation of the anti-Epstein-Barr virus activity of compound 1-10: adopted at first The cellviabilityreagent kit (thermofisher) detects the toxic effect of the compound on the cells; then use the B95-8 cells with EBV virus as a model to evaluate the lytic replication of the compound to EBV, use TPA (20ng / mL) to activate EBV to enter the lytic replication, and pass The QPCR method was used to detect the EBV gene copy number to evaluate the effect of the compound on the replication.

[0067] Evaluation of the anti-KSHV activity of compound 1-18: first adopted The cellviabilityreagent kit (thermofisher) was used to detect the toxicity of the compound to the cells; then the cell line iSLK.219 of the KSHV virus with the recombinant GFP gene latently present was used as a model for evaluating the cleavage and...

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Abstract

The invention provides compounds with EB (Epstein-Barr) virus and Kaposi's sarcoma-associated herpesvirus (KSHV) resisting activities and sources thereof, and a separation purification method and application of the compounds. The compounds 1-18 are separated and purified from Hypericum japonicum, wherein the compounds 1-10 are separated and purified from Hypericum japonicum collected in Dabieshan in Qichun County of Hubei Province in October, and the compounds 11-18 are separated and purified from Hypericum japonicum collected in Lushan in Lushan City of Jiangxi province. The research of the antivirus activities of the 18 compounds on the two tumorigenic herpesviruses detects that the compounds 1, 3, 4, 7 and 8 have inhibiting activities for DNA replication of EB virus; and the compounds 3, 6 and 13-17 have inhibiting activities for replication of KSHV in the pyrolysis stage.

Description

technical field [0001] The invention belongs to the technical field of medicine, and relates to an anti-Epstein-Barr virus and anti-Kaposi's sarcoma-associated herpes virus compound compound and its separation and preparation method and application, in particular to the separation and purification process, structure confirmation, anti-virus and anti-viral DNA replication effects and the like. Background technique [0002] Epstein-Barrvirus (Epstein-Barrvirus, EBV) belongs to the Herpesviridae Gammaherpesvirinae subfamily, is a human lymphotropic virus, and about 95% of healthy adults worldwide carry the virus. EBV is mainly latent in mature B lymphocytes in a quiescent state in humans. It is the pathogen of infectious mononucleosis and is related to the occurrence and development of a variety of human lymphocytic and epithelial cell tumors, such as nasopharyngeal carcinoma (NPC). ), Hodgkin's disease, T / B lymphocytoma, Burkitt'S lymphoma, and B lymphocytoma in immunodeficien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/94C07D493/04C07D313/08C07C49/83A61K31/335A61K31/352A61K31/12A61P31/12A61P31/22
CPCC07C49/83C07D311/94C07D313/08C07D493/04
Inventor 张勇慧胡琳珍薛永波张锦文王珍珍
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