108 results about "Transformation cell" patented technology

The present invention provides efficient transfer of genes into host cells or embryos to transform the cells or embryos by transposition vectors using the minimal amount of nucleotide sequences in the transposon piggyBac required for gene transfer. The transformed cells or embryos may also be developed into transgenic organisms.

The invention is generally related to methods of generating plants transformed with novel autonomous mini-chromosomes. Mini-chromosomes with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the mini-chromosome into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the mini-chromosome. Plants generated in the present invention contain novel genes introduced into their genome by integration into existing chromosomes.

The invention discloses a soybean gene GmCIB1 and a gene GmCRY2 and application thereof for regulating and controlling flowering and aging, and particularly relates to a soybean gene GmCIB1 and a gene GmCRY2 with sequences shown in SEQ ID NO: 1 or 3, a DNA (deoxyribonucleic acid) molecule containing the genes, a carrier, protein encoded by the gene and related transformation cells and transgenic plants. The invention also relates to application of the genes in regulating and controlling plant leaf aging and flowering, and a method for culturing related transgenic plants.

The present invention provides one method of using methanol utilizing yeast, especially transferred recombinant saccharomycete with ectogenic S-adenosyl-menthionine (SAM) synthetase expression vector, to produce SAM and the method of constituting the expression vector and transferred cell. The recombinant saccharomycete is used in fermentation of culture medium with methanol, proper amount of methionine and certain nitrogen source, high yield of SAM may be obtain and SAM content in cell may reach 10-20 % of dry cell weight. The present invention also provides one methanol utilizing Pachia pastoris sacchromycete strain with preserving number of CGMCC No.0648.

The invention discloses a high-efficiency digital optical fiber CDMA (Code Division Multiple Access) repeater and a realizing method. The system consists of a near terminal device and a remote terminal device, and the near terminal device and the remote terminal device communicate with each other through transmitting digital base band signals in optical fibers. The repeater comprises a radio frequency small signal cell, a digital signal process cell, a highly efficient power amplification cell, a duplexer cell, a voltage transformation cell, a monitor cell and the like, wherein the highly efficient power amplification cell adds peak clipping and self-adaptive digital predistortion processes to the digital signal process cell by a symmetric Doherty technology so that the whole device achieves qualified linearity while improving the efficiency. The invention has the beneficial effects of effectively guaranteeing that the linearity index meets the requirements and solving the contradiction of linearity and efficiency of the radio frequency when improving the efficiency of the whole system, and also tracking the power amplification linearity feature changes due to the changes of work conditions and work environments, such as device aging, temperature change and the like, so as to always keep great linear effect.

The present invention provides one new kind dahlia Streptoverticillium resisting receptor protein (S1Vc1) expressed in Solanum lycopersicoides Dun. and its coding sequence and application in improving plant disease resistance, especially in resisting verticillium wilt. The present invention relates to fusion gene constituent and new recombinant expression vector carrying the constituent, as well as transgenic plant generated via transforming plant cell with the expression vector and its subsequent generation, including plant seed and plant tissue. The present invention expresses the said gene in plant to obtain transgenic plant with strengthened resistance to plant diseases, especially verticillium wilt.

The invention belongs to the field of plant genetic engineering and transgenic breeding, and provides a method for culturing and identifying a recombinant vector containing a CmWRKY48 gene, a transformed cell and CmWRKY48 trans-genetic chrysanthemum morifolium and application thereof. The method for culturing CmWRKY48 trans-genetic chrysanthemum morifolium comprises the following steps: (1) cloning a CmWRKY48 gene of chrysanthemum morifolium; (2) constructing a plant expression vector pMDC43-CmWRKY48; (3) transforming the chrysanthemum morifolium by an agrobacterium EHA105 mediated leaf disc method. PCR (Polymerase Chain Reaction) and quantitative RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detections on the transformed plant prove that the CmWRKY48 gene is integrated to the DNA of the genome of the transgentic plant and performs transcription. An anti-aphis detection is performed on the transgenetic plant, and the anti-aphis capability of the transgenetic plant is greatly improved, so that a novel and practical method is provided to breeding of stress tolerance variety of chrysanthemum morifolium by utilizing a gene engineering technology, and the progress of chrysanthemum morifolium biotechnological breeding can be effectively promoted.

The invention belongs to the technical fields of molecular biology and genetic engineering, relates to a new Late Embryogenesis Abundant protein ('LEA protein' for short) which is expressed in the Jatropha curcas, and an encoding sequence and application in improving drought tolerance of plants, and relates to fusion gene structures containing the genes, new recombined expression vectors carrying the fusion gene structures, and transformation plant cells, and transgenic plants of the genes generated by the transformation cells and offsprings thereof, which comprise plant seeds and plant tissues. By introducing the genes into plant hosts, drought stress resistance enhanced transgenic plants (crops) can be obtained. The genes have important theoretical and practical significances for culturing new breeds of drought resistance enhanced crops, and can be applied to breeding and identifying plant resistance breeds needed by farming and animal husbandry and ecological environment management.

The present invention provides recombinant viruses which replicate the viral genome selectively in response to the intracellular conditions of the target cell through the use a pathway-responsive promoter which substantially inhibits viral replication in the host cell based on the phenotypic or genotypic of the infected cell. In the target cell, the promoter element of the pathway-responsive promoter is inactive and thus the virus is permitted to replicate. This results in: (1) killing the cells by natural lytic nature of the virus, and / or (2) provides a therapeutic dose of a transgene product (amplified in comparison to replication incompetent vectors) to the target cell, and (3) producing a localized concentration of the virus facilitating the infection of surrounding cells to the recombinant virus. The invention further provides therapeutic and diagnostic methods of use of the vectors, pharmaceutical formulations comprising the vectors, methods of making the vectors and transformed cells comprising the vectors.