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84results about How to "Improve replication efficiency" patented technology

Data transmission method, data receiving method and sotring equipment

The embodiment of the invention provides a data transmission method, a data receiving method and storing equipment. The emobodiment comprises that first storing equipment receives a first data order sent by a host; the first data order carries to-be-written in data and address information; a first code is added to the to-be-written in data and address information and buffer emory is written in; the first code is a current time slice code; to-be-written in data and address information corresponding to the first code is read from the buffer; the current time slice code is rewritten to mark follow-up information carried by data order; and the to-be-written in data and address information are sent to second storing equipment. Data copying effiency can be improved.
Owner:HUAWEI TECH CO LTD

Method for cultivating hepatitis e virus

The invention discloses a method for cultivating hepatitis e virus. The method comprises the steps that a serum of a later pregnant woman is added into an HEB strain obtained through separation of an epidemic disease investigation for in-vitro culture, the virus can be replicated massively, and immunofluorescence and Western Blot analysis are utilized for revealing that the serum of the later pregnant woman can promote massive replication of HEV progeny virus. According to the method, great breakthrough is made in the aspect of HEV in-vitro culture, and a good foundation is laid for further researching the biological and immunological characteristics of HEV and the HEV infection and pathogenic mechanism and screening and identifying anti-HEV drugs.
Owner:KUNMING UNIV OF SCI & TECH

A message forwarding method and apparatus

The embodiment of the invention provides a message forwarding method and a device, which are applied in the field of communication technology, can reduce the consumption of CPU resources in the message copying process and improve the efficiency of the message copying. Specifically, the scheme is applied to a message forwarding device connected with each member of the stack through an aggregation group. The port of the message forwarding device connected with each member of the stack is a member port of the aggregation group. The message forwarding device further comprises a switching unit. Themessage forwarding method comprises the following steps: the first service unit receives the LACP message sent by the stack member through the member port belonging to the aggregation group locally,adds the identification of the aggregation group to the LACP message, and broadcasts the LACP message through the exchange unit. The second service unit receives an LACP message from the switching unit, searches for a member port belonging to the aggregation group locally according to the identification of the aggregation group in the LACP message, and sends the LACP message through the member port belonging to the aggregation group locally.
Owner:MAIPU COMM TECH CO LTD

Method and device for copying image file

The embodiment of the invention discloses a method and a device for copying an image file. The method comprises the following steps of when the image file of a production center is copied to a data backup center, obtaining the target position information of a copied data block of the current image file; when a write request on the image file is received, and the number corresponding to the starting position of the write request is greater than the number corresponding to the target position, directly executing the write request; when the number corresponding to the starting position of the write request is smaller than the number corresponding to the target position, executing the write request, and recording the write request into a pre-established write request log; when the copying of the image file of the production center to the data backup center is completed, transmitting the data of the write request log to the data backup center, and replaying, so as to form an effective image snapshot. The method disclosed by the embodiment has the advantages that the influence to the I/O (input/output) request operation of an upper layer is low in the image file copying process, the log copying operation is reduced by one half, and the copying efficiency of the image file is improved.
Owner:SANGFOR TECH INC

Drug screening system based on recombinant Zika virus expressing green fluorescent protein and application thereof

The invention discloses a drug screening system based on a recombinant Zika virus expressing a green fluorescent protein and an application thereof. The drug screening system is composed of the recombinant Zika virus (reported zika virus) expressing the green fluorescent protein (GFP) and a BHK-21 cell line expressing DC-SIGNR. The reported Zika virus expresses the green fluorescent protein, has strong fluorescent signal, has uniform coloration in cytoplasm, and is easy to observe and prone to high throughput screening analysis. The BHK-21 cell line stably expressing DC-SIGNR increases the sensitivity to the infection of the reported Zika virus, increases the efficiency of virus replication, reduces the pressure of virus replication, and improves the stability of recombinant virus. The drug screening system can directly observe and analyze the viral replication condition before and after drug action in the state of living cells, and does not need to fix and immunostain cells, so that the whole operation process is simpler and faster, the operation steps of live viruses are reduced, and the safety of the whole operation process is improved.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI

Expression quantity of increasing insect rhabdovirus system exogenous gene by utilizing insect hormone

A process for increasing the expression to exogenous gene in the insect baculovirus system by insect hormones features that the molting hormone (MH), the juvenile hormone (JH) and their mixture are used to increase the expression efficiency of exogenous gene and polyhedron protein gene in the insect baculovirus system and the copying efficiency of insect baculovirus in insect cells.
Owner:中国农业科学院蚕业研究所

Breakpoint re-copying method for medium data

The invention discloses a breakpoint re-copying method for medium data. The method comprises the following steps: a system generates task information according to characteristics of the medium data and begins to transmit the medium data; when the transmission of the medium data is interrupted, the system records state information of a stopping point and adds the state information of the stopping point and the characteristics of the medium data to an unfinished task list; when the new medium data transmission task is started by the system, the systems compares the characteristics of the new medium data transmission task and the characteristics of the medium data in the unfinished task list; if a comparison result is that the characteristics of the new medium data transmission task are as same as the characteristics of the medium data in the unfinished task list, the system extracts the information of the unfinished task and then continues the transmission of the medium data according to the state information of the stopping point; and if the comparison result is that the characteristics of the new medium data transmission task are different from the characteristics of the medium data in the unfinished task list, the system adds the new medium data transmission task to the unfinished task list and then starts the new medium data transmission.
Owner:PANSAFE SOFTWARE SHANGHAI

Construction method of HBV (Hepatitis B Virus) isolating strain replicating-form plasmid

The invention discloses a construction method of HBV (Hepatitis B Virus) isolating strain replicating-form plasmid, comprising the following steps of: 1, extraction of HBV full-length genome, PCR (Polymerase Chain Reaction) amplification and clone construction; 2, construction of a recombinant transition vector; 3, enzyme cutting and dephosphorylation of the recombinant transition vector; and 4, construction of replicating-form plasmid, wherein the recombinant transition vector is synchronously and directionally connected by three connection segments, two reverse BspQI enzyme cutting sites are formed between two inserted HBV segments, and the clone of the HBV full-length genome and the bacterial colony cultivation and amplification of the replicating-form plasmid are carried out by an SOC culture medium. The invention applies a novel full-length genome clone vector, improves the enzyme cutting solution and adopts a novel bacterial colony culture medium. The invention has the advantages of simple performance, high efficiency and stability, can be applied to the comprehensive phenotype research on a China HBV clinic isolating strain whole genome and has very important application value.
Owner:RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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