287 results about "Tropism" patented technology

Methods of making and using recombinant AAV vectors and virions for gene delivery to the lung are described. The recombinant AAV virions are derived from caprine AAV and bovine AAV, both of which display tropism for lung tissue.

Adenovirus serotypes differ in their natural tropism. The adenovirus serotypes 2, 4, 5, and 7 all have a natural affiliation towards lung epithelia and other respiratory tissues. In contrast, serotypes 40 and 41 have a natural affiliation towards the gastrointestinal tract. The serotypes described, differ in at least capsid proteins (penton-base, hexon), proteins responsible for cell binding (fiber protein), and proteins involved in adenovirus replication. This difference in tropism and capsid protein among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins.

The present invention provides methods of achieving directed evolution of viruses by in vivo screening or “panning” to identify viruses comprising scrambled AAV capsids having characteristics of interest, e.g., tropism profile and / or neutralization profile (e.g., ability to evade neutralizing antibodies). The invention also provides scrambled AAV capsids and virus particles comprising the same.

Methods of making and using recombinant AAV vectors and virions for gene delivery to the lung are described. The recombinant AAV virions are derived from caprine AAV and bovine AAV, both of which display tropism for lung tissue.

An equipment for making abrasive particles in even distribution, array pattern and preferred orientation comprises an adsorbing releasing apparatus being composed of a plurality of acicular soft magnetic metallic sticks, an upper and a lower stators, and a plurality of windings. Each winding forms an electric circuit through a electrical source, a resistance, and a switch a plurality of blind holes being distributed on face of the blind hole feeding tropism board. Using electric current to form magnetic field for the acicular soft magnetic metallic sticks absorb or release abrasive particles plated with soft magnetic materials. The equipment is simple, convenient in operation, high in efficiency and reliability. The equipment makes abrasive particles in matrix with an ideal state in distributing density, uniformity, arrange, and tropism. Thereby, the function of the abrasive materials is fully exerted and the life of products is prolonged.

A change in viral tropism occurs in many HIV positive individuals over time and can be indicated by a shift in coreceptor use from CCR5 to CXCR4. The shift in coreceptor use to CXCR4 has been shown to correlate with increased disease progression. In patients undergoing HAART, the predominant populations of virus can be shifted back to CCR5-mediated entry after the CXCR4-specific strains have emerged. The present invention relates to a diagnostic method to monitor coreceptor use in the treatment of human immunodeficiency virus (HIV) infection. The present invention further relates to a diagnostic method applied to HIV-positive individuals undergoing HAART to monitor the suppression of CXCR4 specific strains. The diagnostic methods can be used to assist in selecting antiretroviral therapy and to improve predictions of disease prognosis over time.

The present invention discloses a pearl nucleus processing method for culturing nucleated pearl which comprise the following steps: (1)Choosing pearl nucleus of 12-17mm diameter, (2) purifying pearl nucleus, washing pearl nucleus with 5-10% dilute hydrochloric acid, then washing repeatedly with limewater until the pearl nucleus is neutral in pH, placing pearl nucleus in boiled water for disinfection; (3) activating the immersed pearl nuleus, placing pearl nucleus in activation liquor and processed pearl nucleus is obtained. The present invention purifies the pearl nucleus and activate the pearl nucleus before transplanting that effectively promote the tropism of pearl oyster to pearl nuclus reduce the cut infection probability of nucleus-transplanting tissue quick the healing rate of wound, promote the forming quality of pearl sac, reduce the surface blemish forming rate of nucleated pearl, promote the yield of nucleated pearl.

Methods to improve the tropism or other features of a virus are disclosed. Such methods can be used to prepare, e.g., DNA or plasmid libraries of variants of a gene encoding a viral capsid or envelope protein having a randomly inserted restriction site, libraries of viral clones with such variant genes with a randomly inserted restriction site or polypeptide sequence targeting a receptor expressed by a specific type of mammalian cells. Described are also methods to prepare mosaic viruses, i.e., viral particles wherein copies of one or more capsid or envelope proteins originate from different sources. These methods can be used to prepare mosaic viruses of a specific mixture of wild-type and mutant proteins, or of different types of mutant proteins.

Novel adeno-associated virus (AAV) vectors in nucleotide and amino acid forms and uses thereof are provided. The isolates show specific tropism for certain target tissues, such as blood stem cells, liver, heart and joint tissue, and may be used to transduce stem cells for introduction of genes of interest into the target tissues. Certain of the vectors are able to cross tightly controlled biological junctions, such as the blood-brain barrier, which open up additional novel uses and target organs for the vectors, providing for additional methods of gene therapy and drug delivery.

Adenovirus serotypes differ in their natural tropism. The adenovirus serotypes 2, 4, 5 and 7 all have a natural affiliation towards lung epithelia and other respiratory tissues. In contrast, serotypes 40 and 41 have a natural affiliation towards the gastrointestinal tract. The serotypes described differ in at least capsid proteins (penton-base, hexon), proteins responsible for cell binding (fiber protein), and proteins involved in adenovirus replication. This difference in tropism and capsid protein among serotypes has led to the many research efforts aimed at redirecting the adenovirus tropism by modification of the capsid proteins.

The invention discloses a similar Chinese herbal medicine search method based on a probability topic model. The method includes the following steps: transforming Chinese herbal medicine information in 'China Great Pharmacopoeia' and 'Chinese Materia Medica' into digital text through an optical character recognition tool; extracting information such as efficacy, property and flavor and channel tropism of Chinese herbal medicines by using a regular expression, and building a Chinese herbal medicine information base; generating corresponding vector spaces according to efficacy, property and flavor and channel tropism attributes of the Chinese herbal medicines respectively, and regulating the vector spaces of the efficacy according to the probability topic model; and at last, calculating similarity of the efficacy, property and flavor and channel tropism attributes between the Chinese herbal medicines according to cosine coefficients, and generating a Chinese herbal medicine similarity database. A user inputs the name of a Chinese herbal medicine, and a system displays the Chinese herbal medicine and similar Chinese herbal medicines visually in the form of a relational graph through searching in the corresponding Chinese herbal medicine pair similarity information database. By means of the similar Chinese herbal medicine search method based on the probability topic model, relevant Chinese herbal medicines can be searched according to attribute similarity, and the method is of great significance for Chinese herbal medicine study and Chinese herbal medicine informatization.

The invention discloses an FP growth algorithm model-based traditional Chinese medicine formula data mining method and system. The system comprises the following steps of: pre-processing recorded traditional Chinese medicine original data; constructing an FP-tree data structure which takes characteristic items in a first transaction set as frequent items; arranging characteristic items corresponding transaction in the first transaction set according to the sequence of the frequent items in the FP-tree data structure, and generating a second transaction set, wherein the second transaction set comprises a plurality of data sets, each data set take included characteristic items as transactions and each transaction comprises the characteristic items, arranged before the characteristic items corresponding to the transactions, in the data set; carrying out an FP-growth algorithm on each data set and generating FP-tree sub-data structures in one-to-one correspondence with the plurality of data sets; and mining association rules between different effects and / or association rules between the mined effects and channel tropisms on the basis of a corresponding relationship between the characteristic items in the different FP-tree sub-data structures. According to the method and system disclosed by the invention, the information interferences in the retrieval process of the users can be decreased.