39 results about "Molting Hormone" patented technology

The invention discloses a compound feed for Scylla serrata, comprising fish meal, shrimp head powder, soybean meal, rapeseed meal, fish oil, soybean phosphatide powder, brewers yeast, choline chloride, molt hormone, bread flour, calcium dihydrogen phosphate, sodium dehydroacetate, butyl hydroxy anisole, astaxanthin, Schizochytrium limacinum, Bacillus licheniformis, a composite feeding promoting agent, a complex vitamin additive, a composite mineral matter additive and a composite adhesive. The feed has balanced nutrition, is resistant to spoilage, has a long storage life, not only reduces feed cost but also promotes the growth and improves the color of the Scylla serrate. In addition, the feed is friendly to environment, has good palatability and feed promoting effect, has high utilization efficiency and good stability in water, and can enhance the immunity and survival rate of scylla serrata. The invention also provides a preparation method for the compound feed for scylla serrata, which is simple in technology, with the function substance in raw material undestroyed in the preparation process.

The scorpion breeding method in nature simulating condition includes providing cultivation pond with different temperature and humidity conditions for scorpion to select suitable inhabiting environment and adopting animal and plant complementing mode; providing composite feed including yellow meal worm, ground beetle, artificially compounded feed and molting hormone; and adopting loam and efflorescent wall soil as breeding soil. The present invention can raise the scorpion yield greatly.

The invention discloses feed capable of promoting molt of crayfish and a preparation method thereof and belongs to the field of agricultural feed. The feed is prepared by mixing rice bran powder, soybean meal, fish meal, seaweed powder, bone meal, squid liver paste, cholesterol powder, tricalcium phosphate, calcium hydrogen phosphate, vitamin D3, tea seed meal, potassium dihydrogen phosphate, bile acid and aquatic complex microbial agent and then performing fermentation. The feed has the advantages that the molting hormone of the crayfish is increased by promoting the crayfish to absorb the high cholesterol in the feed to synthesize ecdysterone so as to increase the molt and growth speed of the crayfish; the feed can promote the molt of the crayfish, increase the immunity of the crayfish, increase the number of intestinal tract beneficial bacteria and purify water; drugs such as hormone and antibiotics are not added into the feed, and the feed is simple to prepare, safe in raw materials, rich in nutrition, scientific in compatibility, good in feeding attraction performance, low in cost and capable of increasing benefits.

The invention discloses feed capable of promoting the molt of Penaeus vannamei and a preparation method thereof and belongs to the field of agricultural feed. The feed is prepared by mixing rice bran, bran, bean cake, minced fish meat, seaweed meal, squid liver paste, shrimp shell meal, salted duck egg, bile acid, radix astragali, Eucommia ulmoides, Adenophora stricta, radix rehmanniae preparata, dendrobium, fructus schizandrae, ginseng leaf, trace element agent and microbial fermentation agent and then performing fermentation. The feed has the advantages that the molting hormone of the Penaeus vannamei is increased by promoting the Penaeus vannamei to absorb the high cholesterol in the feed to synthesize ecdysterone so as to increase the molt and growth speed of the Penaeus vannamei; the feed can promote the molt of the Penaeus vannamei, increase the immunity of the Penaeus vannamei, increase the number of intestinal tract beneficial bacteria and purify water; drugs such as hormone and antibiotics are not added into the feed, and the feed is simple to prepare, safe in component, rich in nutrition, scientific in compatibility, good in feeding attraction performance and low in cost.

The invention relates to a feed additive for promoting growth of bred shrimps and crabs after shelling. The feed additive is prepared by screening the following components with an 80-mesh sieve and uniformly mixing the components in percentage by weight: 2 to 5 percent of beta-molting hormone, 3 to 15 percent of antibacterial peptide, 5 to 10 percent of yeast, 10 to 20 percent of bacillus subtilis and the balance of maize starch, wherein the molecular structure of the molting hormone is C27H44O7 and high performance liquid chromatography (HPLC) is more than or equal to 85 percent; the total number of viable bacteria in every gram of yeast is more than or equal to 50 hundred millions; and the total number of viable bacteria in every gram of bacillus subtilis is more than or equal to 200 hundred millions. The feed additive is preferably prepared by screening the following components with the 80-mesh sieve and uniformly mixing the components in percentage by weight: 5 percent of beta-molting hormone, 12 percent of antibacterial peptide, 8 percent of yeast, 15 percent of bacillus subtilis and 60 percent of maize starch, wherein the molecular structure of the molting hormone is C27H44O7 and high performance liquid chromatography (HPLC) is more than or equal to 85 percent; the total number of viable bacteria in every gram of yeast is more than or equal to 50 hundred millions; and the total number of viable bacteria in every gram of bacillus subtilis is more than or equal to 200 hundred millions. The feed additive has a rational formula, the preparation method is simple, and metabolism and in-vivo protein synthesis of the bred shrimps and crabs are promoted through the synergistic effect of the components, so that the autoimmunity and the digestion functions of the shrimps and the crabs can be improved while the shrimps and the crabs are shelled, the growth is promoted, the survival rate is increased and the economic benefit is obviously improved.

The invention discloses a growth promoting premix compound for shrimps and crabs. The growth promoting premix compound comprises the following components in percentage by weight: 0.01-0.05 percent of L-carnitine raw material, 1-2 percent of lecithin raw material, 2-4 percent of inositol raw material, 4-5 percent of glycine raw material, 3.5-5 percent of lysine raw material, 11-15 percent of potassium chloride raw material, 0.5-0.9 percent of molting hormone raw material, 1-8 percent of vitamin C raw material, 1-5 percent of astragalus polysaccharides raw material and 58-73 percent of glucose raw material. A preparation method of the growth promoting premix compound for shrimps and crabs comprises the following steps of: weighing the raw materials according to the weight ratio; adding intoa mixer for mixing for 5-10 minutes till the uniformity is up to 5 percent to obtain a mixture; and packaging. By adopting the premix compound, the stress resistance of shrimps and crabs can be improved, the feed utilization ratio is increased, and the dehulling and growth of shrimps and crabs are facilitated simultaneously.

The invention provides a method for producing plate cocoons and white muscardine silkworms simultaneously. The method mainly comprises the following steps: after silkworms are raised to fifth-instar silkworms, according to fungus strain implantation nodes, beauveria spores are implanted into silkworm bodies, when matured silkworms appear, mulberry leaves which are sprayed with a molting hormone are applied to feed the silkworms, when the silkworms spin and begin to have a little urination, the silkworms are laid on a spinning bed, the angle of the spinning bed is adjusted to make the silkworms crawl uniformly and spin, when silkworms close feet and fall, all silkworms are swept from the spinning bed, the swept silkworms are put into a bamboo basket uniformly, the plate cocoons are collected, the bamboo basket is put into a white muscardine silkworm producing room which has a temperature and humidity until muscardine silkworms are white, baking or drying in the sun is performed, and therefore the white muscardine silkworm finished products are obtained. According to the method, production of the plate cocoons and production of the white muscardine silkworms are combined, so that the yield of the plate cocoons can be guaranteed, economic benefits of producers are improved, and meanwhile the rigidification rate of the produced white muscardine silkworms is high, the cleanness degree of stiff silkworm is high, and drug efficacy meets requirements of Chinese pharmacopoeia.

The invention discloses locust / kochia scoparia immature stem leaf composite crab feed which is characterized by being prepared from the following raw materials in parts by weight: 23-30 parts of rapeseed meal, 19-23 parts of rice, 13-15 parts of starch, 18-25 parts of wheat middling, 1-3 parts of vanilla powder, 0.4-0.7 part of molt hormone, 18-24 parts of kochia scoparia immature stem leaf, 6-8 parts of shrimp paste, 23-31 parts of locust, 15-18 parts of inkfish, 6-9 parts of olive oil, 19-24 parts of gigartina, 16-18 parts of carrot, 17-21 parts of exidia auricula judae, 0.1-0.3 part of angelica sinensis, 0.2-0.5 part of isatis root, 0.2-0.3 part of root of red-rooted salvia, 0.3-0.5 part of root of common peony, 3-6 parts of a phagostimulant and a defined amount of water. The feed is complete and rich in nutrition and good in palatability; by utilizing the locusts and kochia scoparia immature stem leaves, the cost is reduced, the antibacterial performance of the feed is improved, and the immunity of crabs is increased; crabs eating the feed provided by the invention are big and wide in body, low in disease rate, obvious in weight gaining effect, and wide in application prospect.

The extraction method of insect molting hormone analog in bulge relates to extraction of botanical insect hormone analog. The extraction method of insect molting hormone analog in bulge is one combining alcohol extraction, water precipitation and ultrasonic extraction. Through ultrasonic vibration and repeated extraction of the residue, the present invention has high extracting rate, high extract purity, simple extraction process and no environmental pollution.

The invention provides an ajuga multiflora bunge regeneration system and a tissue culture method of a rapid propagation method, belonging to the technical field of in-vitro propagation of plant seedling and laying a good foundation for a cell or tissue culture technology which takes plant molting hormone analogues as target products. The method comprises the following steps of: respectively taking a culture medium as a raw material and doping 1mg of 6-BA and 0.2mg of NAA to prepare a mud-induction culture medium, wherein the culture medium is an MS culture medium and the pH value is 5.8 to 6.5; doping 1mg of 6-BA and 0 to 1mg of 2,4-D to prepare an induced healing culture medium; doping 0 to 2mg of 6-BA to prepare an auxiliary-bud enrichment culture medium; doping 0 to 1mg of IBA (NAA) to prepare a rooting culture medium; selecting leaves and carrying out induced culture after asepsis, wherein the leaves can germinate and grow strong new buds after 30 to 35 days; then carrying out enrichment culture for 25 to 30 days, carrying out rooting culture for 25 to 30 days to form seedlings, and transplanting, wherein the survival ratio reaches more than 95 percent. The ajuga multiflora bunge tissue culture and rapid propagation method aims at the characteristics of ajuga multiflora bunge, is simple, convenient and easy to practice and free of deformity of tissue culture seedlings and has improved propagation coefficient, short propagation period, good culturing effect and high survival ratio.

The invention relates to a recombined insect molting hormone inactivation gene Bbsp::egt and an insecticidal fungistatic agent thereof. The recombined insect molting hormone inactivation gene Bbsp::egt contains coding beauveria bassiana chitinase signal peptide sequences (Bbsp) and coding lymantria dispar nuclepolyhedrovirus (hereinafter referred to as LdMNPV) ecdysteroid uridine 5minute-diphosphonic acid-glucosyltransferase gene (egt), and has a nucleotide sequence as shown in SEQ ID NO.1 and an amino acid sequence as shown in SEQ ID NO.2. The molecular biological technique is adopted to shift into beauveria bassiana to obtain correct genetic transformation bacterial strains. The recombined Bbsp::egt bacterial strain obtained by the invention can disturb the normal operation of a hormone system in vivo of insects, and is beneficial to reducing immune resistance of insects on insecticidal fungi beauveria bassiana and accelerating knockout of fungus on hosts, so that the insecticidal fungi virulence is improved.

The invention discloses pellet feed for eriocheir sinensis. A technical solution lies in that the pellet feed is composed of aquatic green feed, soybean dregs, corn, rice bran, sesame seed meal, small fishes, snail, natural mineral substances, fish meal, silkworm chrysalis, white atractylodes rhizome Chinese herbal medicine feed additives, salt and molt hormone. The pellet feed for eriocheir sinensis completely simulates ecological feed growth conditions required by eriocheir sinensis, and has good palatability and stable performances in water. The particle state can be kept for a long time; repayment of the feed is high; and cost is low. The eriocheir sinensis fed by using the pellet feed for eriocheir sinensis has low incidence of diseases, high survival rate and fast growth speed. The weight of one eriocheir sinensis is higher than that of a wild eriocheir sinensis. The shell of the eriocheir sinensis is bluish white; meat quality is fine and tender; and the pellet feed is environment-friendly. The pellet feed is suitable for eriocheir sinensis at various growth stages.

A process for increasing the expression to exogenous gene in the insect baculovirus system by insect hormones features that the molting hormone (MH), the juvenile hormone (JH) and their mixture are used to increase the expression efficiency of exogenous gene and polyhedron protein gene in the insect baculovirus system and the copying efficiency of insect baculovirus in insect cells.

The invention provides a functional fresh water crayfish feed and a preparation method and a use method thereof. The functional fresh water crayfish feed is prepared from the following raw material components in parts by weight: 10-20 parts of bean meal, 10-15 parts of sunflower kernel meal, 13-20 parts of Canada rapeseed meal, 5-15 parts of peanut meal, 15-20 parts of high gluten flour, 10-20 parts of fish meal, 3-5 parts of shrimp shell powder, 2-3 parts of squid paste, 3-5 parts of pine needle powder, 3-5 parts of spirulina powder, 1-2 parts of a yeast extract, 1-2 parts of phospholipid oil, 2-3 parts of fish oil, 1-2 parts of monocalcium phosphate, 0.5-1 part of a Chinese herbal medicine additive, 0.1-0.2 part of choline, 0.05-0.15 part of a molting hormone, 0.5-1.0 part of complex vitamin, and 0.5-1.0 part of complex mineral. The functional freshwater crayfish feed has more comprehensive and balanced nutrition, and crayfishes grow fast, and have better meat sense and taste.

The invention provides a compound preparation for promoting the shedding and growth of shrimps and crabs and a preparation method thereof. The compound preparation consists of 0.5 to 0.9 weight part of molting hormone, 40 to 60 weight parts of oriental waterplantain rhizome, 65 to 85 weight parts of gentian, 70 to 80 weight parts of common selfheal fruit-spike, 20 to 30 weight parts of glucan and 20 to 30 weight parts of zeolite. The preparation method comprises: weighing the molting hormone, the oriental waterplantain rhizome, the gentian, the common selfheal fruit-spike, the glucan and the zeolite, which serve as raw materials, in weight part; and uniformly stirring the raw materials to obtain the compound preparation. Through the synergy of the component, the compound preparation for promoting the shedding and growth of shrimps and crabs can promote the metabolism and in-vivo protein synthesis of shrimps and crabs to allow shrimps and crabs to shed in time and can improve the immunity and digestion of shrimps and crabs, promote growth and improve survival rate; and the preparation method is simple and meets the needs for large-scale industrial production.

The invention relates to a technology for extracting beta-molting hormone from tussah in a solvent-assisted high-voltage pulsed electric field. The technology is characterized by comprising the following steps of: (1) pretreatment of raw materials; (2) crude extraction; (3) extraction in the high-voltage pulsed electric field: adding 30% of acetic acid solution according to a ratio of feed to liquid being 1:(18-22), placing the mixed liquid in the high-voltage pulsed electric field, and extracting for 1-2 hours at 35-45 DEG C, the electric field intensity of 8-10kv.cm<-1> and pulse number of 8-12 to obtain an extracting solution; and (4) purification: filtering the extracting solution under reduced pressure, and separating and extracting with macroporous resin to obtain the beta-molting hormone. The technology for extracting the beta-molting hormone from the tussah in the solvent-assisted high-voltage pulsed electric field is low in extraction temperature and short in extraction time, ensures the stability of beta-molting hormone extraction and has the advantages of time saving, high efficiency and stability; and simultaneously the yield of the beta-molting hormone is obviously improved.

The invention discloses a molting hormone extracting method. According to the method, methyl alcohol or a methyl alcohol-acetone mixed solution is used to soak cyanotis arachnoidea to extract moltinghormone, and then the molting hormone is obtained through decoloring, crystalizing and recrystallizing. The method is simple and easy to operate, does not need column chromatography, is low in cost and short in period, and is suitable for industrial application and market promotion.

The invention provides a molting hormone regulatory factor E78-C gene cDNA of cotton bollworm. A molting hormone induction factor E78-C gene is cloned from the cotton bollworm; a primer is designed for the molting hormone induction factor E78-C gene of the cotton bollworm; the 658th to the 1122nd in the SEQ ID No. 1 are used as an E78-C target gene fragment to synthesize dsRNA, and then the synthesized dsRNA is transferred to the body cavity of the cotton bollworm by microinjection; a green fluorescent protein (GFP) is used as a control group; and the counting of the egg laying amount of the cotton bollworm injected with dsRNA indicates that when the E78-C gene of the cotton bollworm is used as an interference target gene to carry out injection interference on the cotton bollworm and transcriptional expression of a silent scene, the ovary development of the cotton bollworm is inhibited, the egg laying amount is reduced, and further the population quantity of the cotton bollworm is controlled. The risk that injurious pests have drug resistance is low. A theoretical basis is provided for the creation of novel biological insecticides and plant pest-resistant genetic engineering.

The invention discloses ass meat / saltwater fish composite crab feed which is characterized by being prepared from the following raw materials in parts by weight: 1-3 parts of ethyl acetate, 2-4 parts of butylated hydroxytoluene, 3-6 parts of tea polyphenol, 13-17 parts of rape pollen, 6-9 parts of saccharose, 5-8 parts of glycine betaine, 15-18 parts of white carbon black, 23-30 parts of oil-tea-cake, 17-19 parts of wheat gluten, 5-9 parts of glycerinum, 4-7 parts of fish oil, 25-32 parts of ass meat, 17-20 parts of saltwater fish, 14-18 parts of rose, 13-15 parts of greenish lily flower, 4-7 parts of honey, 16-18 parts of sweet potato, 21-24 parts of water chestnut, 0.04-0.07 part of molt hormone, 3-7 parts of a phagostimulant and a defined amount of water. The ass meat / saltwater fish composite crab feed is balanced and full in nutrition, rich in multiple mineral components, good in palatability, high in digestibility, easy to store and low in price, can promote the growth of the crabs and has less pollution to the environment.

The present invention discloses an anti-stress compound preparation. The anti-stress compound preparation comprises the following components in parts by mass: 20-25 parts of glycine, 10-15 parts of methylenetetrahydrofolic acid, 10-15 parts of calcium lactate, 2-5 parts of vitamins, 1-3 parts of molting hormones, 1-3 parts of potassium ferrate, 5-9 parts of polyaluminum chloride and 1-3 parts of sodium hypochlorite. The present invention also discloses a preparation method of the anti-stress compound preparation. The compound preparation is polymerized to form glucose in aquaculture water bodyand can remain in the aquaculture water for a long time; the excessive glycine can be directly ingested by shrimps to improve the immunity of the shrimps; the calcium lactate ensures supplement of calcium in the shelling process of the shrimps; besides, the molting hormones promote the concentration shelling of the shrimps in the same aquaculture water body, avoid the occurrence of death after the shelled shrimps are attacked by the same kind for food; the compound preparation disinfects the water body, avoids produced lesions and stress due to excessive pathogenic bacteria during the shelling of the shrimps; and the potassium ferrate can effectively remove algae substances in the aquaculture water body and increase the oxygen content in the aquaculture water body.

The present invention provides a completely novel molting hormone receptor, which is used for efficiently screening a substance that can be applied to a disinfestant or the like. An insect molting hormone receptor comprising the following polypeptide (a) and polypeptide (b) or (c): (a) a polypeptide having the amino acid sequence shown in SEQ ID NO: 1; (b) a polypeptide having the amino acid sequence shown in SEQ ID NO: 2; and (c) a polypeptide having the amino acid sequence shown in SEQ ID NO: 3.

The invention discloses a method for breeding and cultivating cyanotis arachnoidea. The method mainly comprises the steps of seed selection and harvesting, seedbed preparation, sowing, seedbed management, field preparation, transplantation, tillage management, harvesting and the like. According to the method, biological characteristics are utilized, control measures for each key planting link are detailed on the basis of a conventional cyanotis arachnoidea planting technology, the fertilization ratio and the planting density are optimized, and a rare earth fertilizer and a biological organic fertilizer are applied according to a reasonable proportion after seedling raising and transplantation, so that the growth of the cyanotis arachnoidea can be effectively promoted, the proportion of nutritional ingredients in plants is optimized, the effects of improving the yield of a unit area and increasing the beta-molting hormone content are achieved under the conditions of reducing the fertilizing amount and expanding a planting row pitch, the planting cost is lowered, the planting return rate is increased, the income increase and the production increase of a farmer are promoted, the development of the cyanotis arachnoidea industry can be effectively promoted, and the method has remarkable economic benefits and social benefits.

The invention relates to a feed for preventing red speck disease of freshwater shrimps. The feed contains the following components: imported fish meal, soybean pulp, cottonseed meal, rapeseed cakes, rapeseed meal, DDGS, alpha starch, phosphorous calcium, choline, adhesive, premix, molt hormone, salt, rapeseed oil and a feed additive. By improving the proportion of the materials, the feed is rich in nutrition, good in palatability and high in utilization rate. The feed for preventing red speck disease of freshwater shrimps contains feed additive component, thus having the efficacies of resisting insects and sterilizing, clearing heat and detoxifying and the like. In the early period of cultivation, the freshwater shrimps are fed with the feed for preventing the red speck disease of the freshwater shrimps for one week, the red speck disease can be effectively prevented, the morbidity of the freshwater shrimps can be reduced, and the cultivation benefits can be guaranteed.

The invention belongs to the technical field of pesticides and discloses an insecticidal composition containing butyl chlorantraniliprole and a molting hormone. The insecticidal composition comprisesan active ingredient A and an active ingredient B at a weight ratio of (50-1):(1-50), wherein the active ingredient A is butyl chlorantraniliprole; the active ingredient B is one of tebufenozide and dibenzoyl-1-tert-butylhydrazine. The composition can be prepared into various agriculturally allowable dosage forms, has a better effect on prevention and control of homona coffearia, rice leaf rollers, diamondback moths and the like, and can effectively retard resistance generation of pests; compared with a single preparation, the composition has a good prevention and control effect, is good in fast-acting property and persistence, and has an obvious synergetic effect; an insecticidal spectrum is enlarged.

The invention discloses a Chinese mitten crab rearing feed. The Chinese mitten crab rearing feed comprises the following raw materials in parts by weight: 15 parts of peanut meal, 20 parts of corn germ meal, 8 parts of fish meal, 4 parts of sesame seed oil extraction waste, 15 parts of soybean meal, 3 parts of condensed fish soluble protein, 4 parts of of xanthan gum, 15 parts of shrimp shell powder, 6 parts of soybean oil, 1 part of calcium dihydrogen phosphate, 2 parts of stone powder, 1 part of chloride choline, 1 part of betaine, 0.4 part of spirulina, 0.1 part of calcium chloride, 0.1 part of lysine, 32 parts of a Chinese herbal medicine additive, 2 parts of phytase, 0.5 part of a compound vitamin additive, 0.3 part of a compound mineral additive, 0.8 part of molting hormone, and 6 parts of endarachne binghamiae. By synchronously adding the Chinese herbal medicine additive, the compound vitamin additive and the compound mineral additive for different feeding ways in different Chinese mitten crab rearing stages, nutrients in the bodies of the Chinese mitten crabs can be adjusted so as to have meat quality and taste of the Chinese mitten crabs improved, immunity of the Chinese mitten crabs enhanced, and yields of the Chinese mitten crabs increased.

A material and a system are provided that efficiently inactivate a molting hormone by means of protein (a) or (b) below, that is, (a) a protein having an amino acid sequence shown by Sequence Listing SEQ ID NO:2, or (b) a protein having an amino acid sequence in which one or a plurality of amino acids are deleted, substituted, or added in Sequence Listing SEQ ID NO:2, the protein having ecdysteroid 22-oxidase activity.

The invention discloses an application of dsRNA of an ecdysone receptor (EcR) related gene USP to control of damage caused by aphids. DsRNA provided by the invention is double-stranded RNA formed by a nucleotide shown in a sequence 2 in a sequence table and a nucleotide shown in an inverse complementary sequence of the sequence 2. Experiments prove that dsRNA of cDNA of the EcR related gene of sitobion avenae is obtained; expression of the gene USP in sitobion avenae can be inhibited, impaired growth and development of wheat aphids can be caused and lethal effects can be generated by adopting the method of feeding dsRNA in vitro.