52 results about "Scylla serrata" patented technology

The invention discloses a compound feed for Scylla serrata, comprising fish meal, shrimp head powder, soybean meal, rapeseed meal, fish oil, soybean phosphatide powder, brewers yeast, choline chloride, molt hormone, bread flour, calcium dihydrogen phosphate, sodium dehydroacetate, butyl hydroxy anisole, astaxanthin, Schizochytrium limacinum, Bacillus licheniformis, a composite feeding promoting agent, a complex vitamin additive, a composite mineral matter additive and a composite adhesive. The feed has balanced nutrition, is resistant to spoilage, has a long storage life, not only reduces feed cost but also promotes the growth and improves the color of the Scylla serrate. In addition, the feed is friendly to environment, has good palatability and feed promoting effect, has high utilization efficiency and good stability in water, and can enhance the immunity and survival rate of scylla serrata. The invention also provides a preparation method for the compound feed for scylla serrata, which is simple in technology, with the function substance in raw material undestroyed in the preparation process.

The invention relates to a compound feed for Scylla serrata, a preparation method, and an application method thereof. The compound feed comprises a solid part and a liquid part, wherein the solid part is composed of fish meal, strong flour, soybean meal, peanut meal, beer yeast, fish oil, shrimp shell powder, cuttlefish paste, phospholipids, plasma protein powder, choline chloride, compound vitamins, compound mineral substances, a decladding element, sodium diacetate, betaine, butylated hydroxyanisole, phytase and beta-carotene. The preparation method of the invention comprises the steps of raw material smashing and sifting, mixing, conditioning, granulating, drying and packaging, ant the like. The feed prepared by the method of the invention has the advantages of good food calling effect, good palatability, high feed utilization, rich and overall nutrient, a raised feed utilizing rate of the Scylla serrata, reduced pool pollution, and significant environmental protection effect.

A Scylla serrata cultivating method relates to a low-salt-density Scylla serrata cultivation in fresh water. The invention comprises that Scylla serrata seed can be used in 2-10 low-salinity, the Scylla serrata pool can contain hidden material in pool to increase the hidden and move space of Scylla serrata to disperse Scylla serrata and reduce meet accidence, thereby improving cultivation survival rate, and the water is 18-30Deg. C and 2-15 salinity in cultivation, the Scylla serrata cultivating density is 300-1800n in each acre, the forge is fish and shellfish to be fed 2-4 times in each day. The invention can improve survival rate and reduce cost.

The invention discloses an ecological breeding method of scylla serrata. At present, main scylla serrata breeding modes include a pond type, an enclosure type and a net cage type, and in the breeding modes, scylla serrata growing together can fight and scramble for food with pincers to lead to mutual killing and injuries, so that breeding survival rate is affected. The method adopts the mode that inner cages are sleeved by an outer cage and one scylla serrata is bred in each inner cage, and includes the technical steps of (1) breeding net cage selection; (2) net cage fixing; (3) scylla serrata breeding; and (4) scylla serrata catching. Since only one scylla serrata is bred in single inner cage, mutual pincers fight of the scylla serrata is avoided, the survival rate is increased, and the scylla serrata growing in the inner cages can eat submarine plankton and small animals on mud flats along with seawater tide rising and falling, and further nutritional requirements of the scylla serrata are guaranteed. The ecological breeding method is capable of increasing breeding density, 1000-1500 scylla serrata can be bred on per mu of mud flat, 400-500 kilograms of scylla serrata / per mu are gained, and accordingly mud flat resources are utilized to the greatest extent.

The invention relates to a fingerling desalination and stocking method for the scylla serrata freshwater culture, in particular to the seawater economic crab culturein freshwater, belonging to the aquaculture technical field. The invention is characterized in that the method comprises the following technique steps: preparing a temporary prawn larva culturing pond; cleaning up the temporary crab larva breeding pond; building anti-escaping facility; setting artificial hidden objects; sterilizing the pond; fertilizing; the pond affusion and salinity regulating; temporary crab larva breeding; crab larva sterilizing; crab larva stocking and breeding; crab larva desalting; crab larva low salinity transferring and desalting; crab larva cultivating; aquatic planting; the freshwater breeding of the crab larva. The invention can prevent the crab larva from dying caused by the sudden change of an osmotic pressure after directly entering the freshwater through the desalting process during the temporary breeding period of the crab larva; leading the scylla serrata to be able to grow from the seawater into the freshwater after artificial desalting and breeding, which is beneficial to reduce the incidence of the scylla serrata, improve the surviving rate of stocking and breeding of the crab larva and increase the culturing yield.

The invention discloses a scylla serrata sesame butter. The sesame butter is prepared form the following ingredients in parts by weight: 60-80 parts of minced scylla serrata meat, 50-60 parts of sesame butter, 10-20 parts of sesame, 30-40 parts of sesame oil, 10-15 parts of fennel, 2-4 parts of pepper, and 15-20 parts of salt. The scylla serrata sesame butter disclosed by the invention not only has the seasoning characteristics of a common sesame butter, but also has the high-nutrition scylla serrata ingredient.

The invention discloses mixed feed for cultivating soft-shell scylla serrata. The mixed feed is prepared from the following components in parts by weight: 35-40 parts of fish meal, 10-15 parts of blood meal, 5-10 parts of shrimp head meal, 20-25 parts of puffed soybean meal, 10-15 parts of peanut meal, 3-5 parts of beer yeasts, 5-10 parts of wheat germ powder, 3-5 parts of fish oil, 3-5 parts of vital gluten, 5-10 parts of rosemary powder, 1-3 parts of calcium dihydrogenphosphate, 0.1-0.2 part of unshelling elements, 0.01-0.03 part of butyl hydroxy anisd, 0.01-0.03 part of butylated hydroxytoluene, 1-3 parts of a weighting agent, 1-3 parts of compound phagostimulant, 0.3-0.5 part of multi-vitamin additives and 0.1-0.3 part of compound mineral additives. The mixed feed disclosed by the invention can promote the scylla serrata to unshell to improve the survival rate and shorten the cultivating period of the soft-shell scylla serrata. The survival rate of the soft-shell scylla serrata is high and the weight gain rate is great, so that the culture benefit is improved. The invention further discloses a preparation method of the mixed feed for cultivating the soft-shell scylla serrata. The preparation method is simple in process step and suitable for industrialized production, and the prepared mixed feed is less in nutritional loss, good in moldability and uniform in granule.

The invention relates to a Scylla serrata seed cultivating pool and relative method, in particular to a method for improving survival rate and growing seed Scylla serrata quickly. the invention comprises a main pool and a seed pool, wherein the bottom of th main pool is provided with a slope inclined along a water outlet, and a sand layer, the sand layer is provided with at least one groove extended from the high part to the lower part of the bottom of the main pool to be collected into the water outlet, the seed pool is arranged under the water outlet with a network box inside. The water specific weight is 1.006-1.015 while the seed cultivating density is 2000-3000n / m2, while the water is exchanged each day and the forge is fish slurry, shellfish meat, or the like to be fed for at least four times and 2-3 times of seed weight, and the whole cultivation is aerated continuously.

The invention discloses methods for detecting bicistronic mRNA viruses of a scylla serrata and kits thereof. A poly-anti-mono monoclonal-antibody and double-antibody sandwich ELISA method and a double-antibody and monoclonal-antibody sandwich ELISA method are adopted for detecting the bicistronic mRNA viruses of the scylla serrata by using monoclonal antibody combined with MCDV specificity. Both the poly-anti-mono monoclonal-antibody and double-antibody sandwich ELISA method and the double-antibody and monoclonal-antibody sandwich ELISA method can sensitively and specifically detect the MCDV masculine in the haemolymph of the virus-infected crabs with the detectable rate of 82 to 92 percent, while the double-antibody and monoclonal-antibody sandwich ELISA method has better sensitivity and specificity on the detection of the bicistronic mRNA viruses of the scylla serrata. The two methods of the invention have the advantages that: corresponding kits can be prepared by the two methods, so the MCDV can be detected with fast speed, high efficiency, sensitivity, specificity and stability, and the methods have instructive meaning for detection in production and future scientific researches.

The invention discloses a method for raising soft-shell scylla serrata indoors, solving the problems that a method for raising the soft-shell scylla serrata, of the prior art, is great in labor intensity, unbeneficial to unshelling the soft-shell scylla serrata, high in death rate of the soft-shell scylla serrata and low in weight gain rate. The method comprises the following steps of (1) selecting a raising pond; (2) selecting the scylla serrata to be raised; (3) sterilizing the raising pond; (4) putting the scylla serrata to be raised into the pond; (5) raising the soft-shell scylla serrata. According to the method for raising the soft-shell scylla serrata indoors provided by the invention, the steps are simple; the labor intensity can be decreased greatly; the scale production is applicable; as the raising conditions and the compound feed are optimally improved, the scylla serrata can become fatter and heavier fast; with the accumulation of sufficient nutrient substances, the unshelling of the scylla serrata is accelerated, the survival rate is improved, and the period of raising the soft-shell scylla serrata is shortened; moreover, the survival rate of the soft-shell scylla serrata is high, the weight gain rate is high and the raising benefits are favorably improved.

The invention provides a fusion expression product of antimicrobial peptide genes of two marine animals and a preparation method of the fusion expression product, relating to fish and crab gene engineering in the biotechnical field. The gene of the fusion expression product comprises a scylla serrata antimicrobial peptide scygonadin2 gene, and connection peptide and large yellow croaker antimicrobial peptide hepcidin genes. The preparation method comprises the following steps of: cloning the fusion gene scygonadin2-hepcidin; expressing the fusion gene scygonadin2-hepcidin; and obtaining the fusion expression product of the antimicrobial peptide genes of the two marine animals. In the preparation method, a pET28a prokaryotic expression vector is utilized to successfully and efficiently express the fusion expression product, which has antibiosis activity, of the scylla serrata antimicrobial peptide scygonadin2 and the large yellow croaker antimicrobial peptide hepcidin is expressed, so that the officinal value of tandem antimicrobial peptides can be developed.

The invention relates to a tilapia and scylla serrata polyculture method which comprises the steps that a pond with unobstructed intake and drainage channels, matched electric power and complete oxygenation facilities is selected and disinfected thoroughly and wholly; then tilapia fry are put in; scylla serrata are put in after 30 days; tilapia complete formula feed is fed two times per day; water body disinfection is performed by quick lime every other 15-20 days in a culture process; water quality regulation is performed by a biological agent; the scylla serrata are subjected to crab pot catch rotation after cultured for 2-3 months; and the pond is dried, and tilapia is caught after cultured for 6 months. According to the method, a unique feasible technical operation route is adopted; the tilapia and the scylla serrata are collocated reasonably due to the life habit and food habit characteristics; a culture water environment is fully exerted; a bait utilization ratio is increased; a bottom environment is improved effectively; mass breeding of harmful microorganisms is reduced; the incidence is reduced; survival rates are increased obviously compared with monoculture; yield increasing and efficiency increasing are achieved; and the method is an efficient, environment-friendly and healthy culture model.

The present invention discloses a method of cultivating scylla serrata. The scylla serrata cultivation technology has the beneficial effects that (1) the present invention provides a method of systematically cultivating the scylla serrata; and (2) the key point of the scylla serrata cultivation technology is to control water temperature and salinity, and the health rate of the cultivated scylla serrata is high.

The invention discloses feed for scylla serrata. The feed is prepared from the following materials in parts by weight: 100-200 parts of fish meal, 50-100 parts of crab-shell and shrimp-shell extract, 30-50 parts of soybean protein powder, 30-50 parts of expanded corn flour, 10-20 parts of oyster shell power, 10-20 parts of earthworm powder, 15-25 parts of bone meal, 20-30 parts of hair weeds, 10-20 parts of pueraria starch, 7-15 parts of seaweed, 20-30 parts of sea algae, 1-5 parts of yeast powder, 50-100 parts of milk and 10-20 parts of additives. The feed disclosed by the invention has the advantages that due to addition of the hair weeds, sea algae and seaweed, natural nutritional components of the feed can be added, and the scylla serrata can be induced to eat more feed; in addition, the pueraria starch is added, so that the bonding property of the materials is improved and the water pollution is reduced; and since multiple vitamins, trace elements and corn germ oil are contained in the additives, the anti-stress capability and the organism immunity of the scylla serrata can be adjusted and improved, and the prevalence rate of the scylla serrata can be reduced.

The invention relates to an ecological breeding method for polyculture of scylla serrata and penaeus japonicus in coastal areas of Northern Jiangsu Province. The method comprises the steps of (1) selection of a breeding pond; (2) disinfection of the breeding pond; (3) application of fertilizer and water, wherein water is fed 10 days before young scylla serrata is released, the water depth is 0.3m,20mg / L of nitrogenous fertilizer and 2mg / L of phosphatic fertilizer are applied, the pond water is dark brown or yellow green or tawny, and the transparency is 40cm; (4) releasing of young penaeus japonicus; (5) throwing of penaeus japonicus feed; (6) throwing of young scylla serrata, wherein when penaeus japonicus grows to 2-3cm, young scylla serrata is thrown according to the density of 300-400young scylla serrata per mu; (7) throwing of scylla serrata feed; (8) water quality management; (9) disease control; and (10) harvesting. By means of the breeding method, the breeding survival rate of megalopa of scylla serrata is not lower than 13%, the breeding survival rate of penaeus japonicus is not lower than 75%, the weight of bred scylla serrata individuals is 350-500g, the weight of bredpenaeus japonicus individuals is 5-10g, diseases are few, the breeding effect is remarkable, and the method has good application prospects.

The present invention discloses a scylla serrata strip tofu, which comprises, by weight, 40-60 parts of mixed and minced fishes, 0.5-1.5 parts of soybean separation protein powder, 8-12 parts of water, 4-8 parts of starch, 4-7 parts of salad oil, 2-5 parts of onion, 15-25 parts of scylla serrata strips, 1-3 parts of salt, 1-3 parts of sugar, 0.15-0.3 part of monosodium glutamate, 0.1-0.2 part of xylose, 0.3-0.5 part of glucose, 0.03-0.07 part of Mirin, and 0.1-0.3 part of skipjack tuna. The present invention further discloses a production method for the scylla serrata strip tofu. According to the present invention, the mixed and minced fishes are adopted as the main raw material, the special process way is matched to process scylla serrata strips, and then deep processing is performed to prepare the scylla serrata strip tofu, wherein the scylla serrata strip tofu has advantages of tender and crisp taste, low fat, more proteins and delicious taste, has characteristics of red and white matched color, significant color contrast, beautiful outlook and appetite attraction, and can be processed into the cuboids through the existing technology so as to meet requirements of a variety of consumer groups and consumer channels.

The invention discloses a non-medicinal treatment method for a sessile ciliate disease of scylla serrata. When a scylla serrata shows a symptom of a sessile ciliate disease, an attachment on a carapace at the back of the scylla serrata is taken to be placed under the (10*10)-time visual field of a microscope for a microscopic examination, when the number of sessile parasites is not less than 50, black breams of which the body lengths are 5 to 10cm and the quantity is 300 to 1200 per mu are put into a pond to breed. After fry are put into the pond, the feeding of both fish and the scylla serrata are stopped for 6 to 13 days, the excrement of the fry of the black breams is gathered, crude protein content is less than 11.63 percent through detection; bottom mud is collected, superficial bottom mud is taken for detection, and total carbon content is less than 214.7mg / Kg. The body surface of the scylla serrata is periodically subjected to the microscopic examination under the (10*10)-time visual field of the microscope, when the quantity of ciliates on the body surface of the scylla serrata is not more than one, the disease is cured. According to the non-medicinal treatment method for the sessile ciliate disease of the scylla serrata, excessive organic matters at the bottom of the pond are eliminated through matching the fry of the omnivorous black breams by utilizing a scylla serrata culturing pond; a nutrition source of breeding the ciliates is cut off; a high-efficiency and environment-friendly method without using a medicine is provided for treating the sessile ciliate disease of the scylla serrata; the non-medicinal treatment method for the sessile ciliate disease of the scylla serrata has favorable popularization and application prospects.

The invention discloses a complex enzyme preparation special for crab formula feeds; the complex enzyme preparation is prepared by solving five monomeric enzymes such as neutral protease, cellulose, amylase, lipase and phytase into a water solution containing composite stabilizer; wherein, the neutral protease is 1000-3000 IU / g; the cellulose is 8000-16000 IU / g; the amylase is 5000-20000 IU / g; thelipase is 10-30 IU / g; the phytase is 500-800 IU / g. Adding 500-1000 g / t of the complex enzyme preparation of the invention on the basis of perfect compound feeds of Eriocheir sinensis, mud crab, bluecrab can obviously improve the rate of live weight growth, yields of crabs and the efficiency of the feeds, and reduce excretion of ammonian and phosphorus, alleviate the eutrophication degree of theaquaculture water, and prevent the infections disorders and improve the survival rate; the invention is a safe green feed additive without pollution nuisances and residuals.

The invention discloses a formula for preparing a soft pellet bait for scylla serrata. According to the formula, the bait comprises the following components in parts by weight: 40 to 50 parts of mixed dry material, 20 to 30 parts of fish mince, and 20 to 30 parts of glutinous rice; the mixed dry material comprises the following components in percentage by weight: 40 to 50% of fish meal, 10 to 20% of cooked groundnut meal, 10 to 20% of cooked soya bean meal, 10 to 20% of wheat middling, 4.5% of shrimp and crab shell powder, 0.4% of fish oil, 0.09% of decorticating agent, and 0.01% of phospholipid. According to the formula for preparing the soft scylla serrata pellet feed, waste soup which is produced by cooking shellfish of a canned shellfish or dried shellfish product processing enterprise is concentrated to obtain shell concentrated liquor which is added to a synthetic formula as a food attractant of synthetic scylla serrata bait, and therefore, the palatability of the synthetic scylla serrata bait and the nutritional ingredients of the bait are greatly improved and increased.

The invention relates to a Scylla serrata reovirus colloidal gold immunochromatographic assay reagent strip and belongs to the field of biological detection. SSRV recombinant antigen p29 expression, purification, monoclonal antibody preparation, polyclonal antibody preparation, colloidal gold immunochromatographic assay reagent strip preparation and sensitivity and specificity of the Scylla serrata reovirus colloidal gold immunochromatographic assay reagent strip on detecting SSRV antigens are included. The invention further relates to a preparing method and a using method of the Scylla serrata reovirus colloidal gold immunochromatographic assay reagent strip. Compared with traditional virus extracting, grinding, centrifuging and the like, the colloidal gold immunochromatographic assay reagent strip established through the method has the advantages of being convenient and fast to use, sensitive, special and the like, a definite diagnosis result can be obtained within 10 minutes, and the colloidal gold immunochromatographic assay reagent strip is especially suitable for clinical use and use in grassroots units.

The invention discloses a method for controlling bacterial diseases during scylla serrata breeding. The method comprises disease prevention and treatment. Specifically, pool water sterilization and prevention in germ high-incidence seasons are conducted for medicine prevention, and medicine soaking and oral medicine administration are conducted for medicine treatment. Compared with the prior art, the method has the advantages that causes and high-incidence seasons of scylla serrata bacterial diseases are analyzed, regular sterilization of breeding water is conducted, prevention medicine is added to feed in the high-incidence seasons to improve the immunity of scylla serrata on bacterial diseases, digestion function is also improved, the ingestion capacity of scylla serrata at high temperature is improved, infected scylla serrata is isolated in time, therapeutic soaking and oral administration are conducted, spreading of bacterial diseases is controlled, recovery treatment is conducted, scylla serrata recovery time is shortened, recovery effect is improved, and the survival rate of scylla serrata is increased greatly.

The invention discloses a formula and a processing technology characterized by adding poached shellfish juice concentrated liquor as an attractant of soft particle baits of scylla serrata. The formula comprises the following components by weight percent: 25% of fish powder, 12% of cooked peanut cake powder, 12% of cooked soybean meal, 10% of wheat middling, 15% of glutinous rice, 15% of chilled fresh offal fish, 5.5% of shrimp and crab shell powder, 0.4% of fish oil, 0.09% of shelling element, 0.01% of phospholipid and 5% of shellfish juice concentrated liquor. The formula and the processing technology have the advantages that the shellfish juice concentrated liquor is prepared by concentrating waste cooking liquor obtained after poaching shellfish in the processing enterprises of canned shellfish or shellfish dried products and is added to the synthetic formula as the food attractant of artificial mixed baits of scylla serrata, thus greatly improving the palatability of the artificial mixed baits of the scylla serrata and increasing the nutritional components of the baits; by utilizing the characteristics of strong adhesion between high-viscosity rice and feeds of fresh and live fish and good stability, the soybean meal is uniformly polymerized in the dextrin molecules of glutinous rice to form a stable bonding state; the baits have high digestive performances and good palatability.

The invention discloses a polyculture method of Scylla serrata and perches, comprising cleaning mud in a pond that uses mixed silt as a base, has smooth water access ditches and has water exchange equipment and aerating equipment, subjecting a pond dike to leak-proof reinforcement, disinfecting, planting algae at the bottom of the pond, setting layers of wire mesh at the bottom, stocking Scylla serrata in the wire mesh, stocking perches above the wire mesh, feeding the upper perches with common perch feed while the lower crabs of Scylla serrata are not specially fed with feed, disinfecting thepond with quick lime at intervals of 15-20 days during culturing, discharging lime water, and re-injecting filtered sea water. The polyculture method of Scylla serrata and perches makes use of livinghabits and preying features of perches and Scylla serrata, the bottom environment is effectively improved, and disease attack chances are decreased; culturing cost is lowered, and Scylla serrata is provided with higher survival rate and better condition.

The invention discloses a formula for preparing soft particle mixed baits of scylla serrata. The formula comprises the following components by weight percent: 30% of fish powder, 10% of cooked peanut cake powder, 10% of cooked soybean meal, 10% of wheat middling, 15% of glutinous rice, 15% of chilled fresh offal fish, 4.5% of shrimp and crab shell powder, 0.4% of fish oil, 0.09% of shelling element, 0.01% of phospholipid and 5% of shellfish juice concentrated liquor. The formula has the advantages that the shellfish juice concentrated liquor is prepared by concentrating waste cooking liquor obtained after poaching shellfish in the processing enterprises of canned shellfish or shellfish dried products and is added to the synthetic formula as the food attractant of artificial mixed baits of scylla serrata, thus greatly improving the palatability of the artificial mixed baits of the scylla serrata and increasing the nutritional components of the baits.

The present invention Scylla serrata antibiotic peptide and its gene and genetic cloning method, and relates to one kind of active antibiotic peptide scygonadin and its gene. Antibiotic peptide scygonadin is separated and purified from male sexual gland of Scylla serrata, and degenerate primer is designed and synthesized based on the total RNA of the male sexual gland as template and the detected 20 amino acid sequence in N end of antibiotic peptide scygonadin and cloned to the whole length cDNA gene sequence one the antibiotic peptide scygonadin by means of RT-PCR, RACE or other molecular biological process. Genetic engineering strain with high efficiency expression of the antibiotic peptide may be constituted via constituting eukaryotic expression vector of the gene so as to externally produce antibiotic peptide as feed additive for aquatic animal to resist drug resisting bacteria. In addition, the present invention may be used also in transgenic blue crab breeding.