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Ajuga multiflora bunge regeneration system establishment and rapid propagation method

A multiflora and system technology, applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve problems such as inability to meet actual production needs and large-scale production requirements, and reduce the number, so as to achieve robust growth of seedlings, good growth effect

Inactive Publication Date: 2010-08-18
NORTHEAST FORESTRY UNIVERSITY +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction and separation of the components of the sinensis at home and abroad are mainly based on natural resources. Due to limited resources, many plants are in an endangered state, and the number has decreased sharply, which cannot meet the actual production needs and large-scale production requirements.

Method used

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  • Ajuga multiflora bunge regeneration system establishment and rapid propagation method
  • Ajuga multiflora bunge regeneration system establishment and rapid propagation method
  • Ajuga multiflora bunge regeneration system establishment and rapid propagation method

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example 1

[0020] Example 1: select the blades of Sclera multiflora as explants for inoculation.

[0021] Preparation of medium:

[0022] Hormones were added to the basic medium MS: BA, 2,4-D, NAA or IBA to prepare bud induction medium, cluster shoot induction medium, callus induction medium and rooting medium respectively, The pH value of these mediums is 5.8, and the thickness of the medium is 1.1-1.5 cm.

[0023] Bud induction medium: MS+6-BA 1mg / L+NAA 0.2mg / L

[0024] Callus medium: MS+6-BA 1mg / L+2,4-D 0~1mg / L;

[0025] Proliferation medium: MS+6-BA 0~2mg / L

[0026] Rooting medium: MS+IBA(NAA) 0~1mg / L

[0027] Bud induction culture:

[0028] Select the leaves, cut the leaves into 1cm×1cm size, and inoculate them on the bud induction medium. The culture conditions are as follows: culture temperature: 25±2°C, light time 16h / d, light intensity 1500-2000Lx, induction culture time 30-35d.

[0029] Callus culture:

[0030] Select the leaves, cut the leaves into 1cm×1cm size, and in...

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Abstract

The invention provides an ajuga multiflora bunge regeneration system and a tissue culture method of a rapid propagation method, belonging to the technical field of in-vitro propagation of plant seedling and laying a good foundation for a cell or tissue culture technology which takes plant molting hormone analogues as target products. The method comprises the following steps of: respectively taking a culture medium as a raw material and doping 1mg of 6-BA and 0.2mg of NAA to prepare a mud-induction culture medium, wherein the culture medium is an MS culture medium and the pH value is 5.8 to 6.5; doping 1mg of 6-BA and 0 to 1mg of 2,4-D to prepare an induced healing culture medium; doping 0 to 2mg of 6-BA to prepare an auxiliary-bud enrichment culture medium; doping 0 to 1mg of IBA (NAA) to prepare a rooting culture medium; selecting leaves and carrying out induced culture after asepsis, wherein the leaves can germinate and grow strong new buds after 30 to 35 days; then carrying out enrichment culture for 25 to 30 days, carrying out rooting culture for 25 to 30 days to form seedlings, and transplanting, wherein the survival ratio reaches more than 95 percent. The ajuga multiflora bunge tissue culture and rapid propagation method aims at the characteristics of ajuga multiflora bunge, is simple, convenient and easy to practice and free of deformity of tissue culture seedlings and has improved propagation coefficient, short propagation period, good culturing effect and high survival ratio.

Description

Technical field: [0001] The invention belongs to the technical field of plant in vitro propagation and seedling formation, and particularly relates to a method for tissue culture and rapid propagation of seedlings. Background technique: [0002] Ajuga multiflora Bunge is an evergreen perennial herb of the Lamiaceae genus Ajuga. Stems quadrangular, with stolons and erect stems. The stem nodes have aerial roots, the leaves are opposite, the edges are coarsely serrated, the apex is blunt, the base is wedge-shaped, the leaves are papery, oblong, and the leaves are wrinkled. The growing season is green with purple, and the leaves are purple in autumn. Blue-purple, cymose inflorescences, open sporadically all year round, relatively concentrated opening time is April-May and October-December, plant height 25-30cm. It has a long flowering period, beautiful plant shape, fast growth, easy cultivation, and great ornamental value. It can be planted in forests and wetlands in patches t...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 迟德富赵晓杰李晓灿宇佳钱晶晶
Owner NORTHEAST FORESTRY UNIVERSITY
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