124results about How to "Broad spectrum antimicrobial activity" patented technology

Polymeric anti-microbial agents produced by substituting the nitrogen atoms in the backbone of ethylenimine polymers are provided. The agents are believed to have low human toxicity while being effective against a variety of pathogens and are useful in applications involving human contact, such as cosmetics, hair care products and textiles, as well as in applications with much less human contact, such as coatings.

The invention provides therapeutic antimicrobial compositions and methods for their use based on natural organic phenolic compounds combined with pharmacological agents. The antimicrobial activities of each carvacrol and thymol are believed to be enhanced, while the pharmacological properties of procaine and related compounds are added to provide their unique properties to facilitate usefulness and effectiveness in humans. The therapeutic compositions are active against bacterial, fungal, and protozoan infections. The forms of the invention are intended to treat various internal infections through parenteral, subcutaneous, intradermal, intravenous, and intramuscular injections. They are also intended as useful agents to treat microbial infections that have become resistant to conventional anitibiotics as well as secondary opportunistic infections.

An anti-microbial preservative for use in ophthalmic and dermatologic products. The preservative includes from about 0.005 wt. % to about 0.20 wt. % chlorite compound and from about 0.005 wt. % to about 0.05 wt. % peroxy compound. Additionally, the preservative does not generate chlorine dioxide within the pH range of 5.0-8.8. Also included are an antimicrobial ophthalmic and dermatologic compositions for direct application onto an eye or skin of a living being including from about 0.005 wt. % to about 0.20 wt. % chlorite compound and from about 0.005 wt. % to about 0.05 wt. % peroxy compound. Also included are methods for treating dryness of the eyes and skin disorders (e.g., wounds, burns, infections, ulcerations, psoriasis, etc.) and for disinfecting and cleansing contact lenses while in place upon an eye by applying the composition to the eye or to the contact lens.

The present invention is based on a plurality of benefits from the extracts of mycelia of individual fungal species, and mixtures of species, to provide an armamentarium of defenses from multiple stressors in order to help bees survive a complex of symptoms collectively called colony collapse disorder (CCD). More particularly, the present invention utilizes specific concentrations of extracts from pure cultured mycelium from mushroom forming fungi to reduce harmful viruses in bees and to increase the longevity of bees.

The invention relates to a multifunction disinfection sterilization detergent combination and the making method. It adopts quaternary ammonium salt, biguanide species, chelating agent, chlorine-hydroxyl diphenyl ether, fatty alcohol compound, organic acid compound, and nonionic reaction compound to make disinfection sterilization detergent. It is non-toxic and without side effect. So it can be widely used in various industries.

The invention relates to genetic engineering of Scylla paramamosain, and provides a Scylla paramamosain anti-lipopolysaccharide factor, and a preparation method and application thereof. The preparation method comprises the following steps: building a recombinant expression vector for the Scylla paramamosain anti-lipopolysaccharide factor; introducing the recombinant expression vector into host cells, and carrying out induced expression on the host cells to obtain an expression product; and separating and purifying the expression product to obtain a recombinant protein, namely the Scylla paramamosain anti-lipopolysaccharide factor. The Scylla paramamosain anti-lipopolysaccharide factor has obvious growth inhibition and sterilization effects on Gram-negative bacteria such as Shigella flexneri, Vibrio alginolyiicus, Vibrio parahaemolyticus, Pseudomonas stutzeri and Pseudomonas fluorescens and Gram-positive bacteria such as Corynebacterium glutamicum, and has a potential application value in the preparation of antibacterial medicaments and antimicrobial additives for animal feed.

The invention discloses a washing composition as well as a preparation method and application of the washing composition. The washing composition is prepared by taking water, alkyl polyglucoside, propylene glycol, alkyl betaine, sodium lauryl sulfate, flos lonicerae extract, chrysanthemum extract, radix sophorae flavescentis extract, mint extract, table vinegar (edible acetic acid), table salt, edible essence and a thickening agent as raw materials, and has the functions and characteristics of sterilization and disinfection, oil removal and stain removal, itching stopping and skin protection, odor removal and deodorization, low foam, and capability of being cleaned by washing; meanwhile, alcohol, an alkali, a preservative and other disinfection components are not added, so that a functional skin-protection daily chemical washing product, which can be used for naturally killing viruses and sterilizing, removing oil and dirt, stopping itching and dandruff, resisting allergy and protection skins, and has a medicine effect, is obtained.

The invention provides a preservation technology for bamboos eaten by pandas, and belongs to the technology field of fresh bamboo preservation. The preservation technology comprises following steps: soaking the bamboo incisions in chitosan coating plastics with a concentration of 0.5 to 2.0%, drying with cold wind then diagonally placing the fresh bamboos with the incisions upward placed at the temperature of 2 to 6 DEG C, steaming the fresh bamboos for 30 to 40 hours with a steaming agent 1-methylcyclopropene with a concentration of 0.5 to 2.0 [mu]L / L, and then storing the fresh bamboos for 15 to 45 days at the refrigeration temperature of 2 to 6 DEG C after the steaming is over. The preservation technology for bamboos eaten by pandas solves the problems that the loss of unique flavors of color, fragrance, taste, and crisp which is caused by the application of chemical preservation technology on bamboos eaten by pandas, and safety hazards existed in conventional chemical antiseptic preservation method, has the advantages of no toxicity, no harm, green, health, strong operability and simple method, and is capable of effectively prolonging the expiration date of the bamboos, and maintaining the original flavor of the bamboos.

The invention discloses an antibacterial peptide and application thereof. The antibacterial peptide is an antibacterial peptide YHX-1 with an amino acid sequence as shown in SEQ ID NO. 1. The antibacterial peptide has broad-spectrum antibacterial activity, can inhibit gram-positive bacteria such as listeria monocytogenes, staphylococcus aureus and streptococcus mutans and gram-negative bacteria such as escherichia coli and salmonella, and has high antibacterial activity. In addition, the antibacterial peptide is relatively low in hemolytic activity, relatively low in toxicity, short in synthetic sequence, small in molecular weight and low in chemical synthesis difficulty, the safety is ensured while pathogenic bacteria in a living body can be well and specifically killed, and in addition, the large-scale production cost can also be saved.

The invention discloses a chitosan hemostatic wound-healing material. The chitosan hemostatic wound-healing material comprises at least two layers of structures, wherein the lower layer is a chitosan rapid hemostatic layer; the upper layer is a chitosan slow release layer; the chitosan rapid hemostatic layer is obtained by evenly mixing a chitosan-acetic acid solution, carrying out cross-linking reaction for 3-6 hours, then adding a starch nano-microsphere solution and a nano-silver solution, mixing evenly, pouring in a mold, pre-freezing, and freezing and drying under vacuum; and the chitosan slow release layer is obtained by evenly mixing a chitosan-acetic acid solution, a calcium chloride solution, a thrombin solution and a nano-silver solution, carrying out cross-linking reaction for 3-6 hours, pouring in a mold, pre-freezing, and freezing and drying under vacuum. The chitosan hemostatic wound-healing material simultaneously has effects of rapidly stopping bleeding and promoting wound healing.

The invention relates to a rana nigromaculata antibacterial peptide and a gene and application thereof, belonging to the technical field of medical technology. The rana nigromaculata antibacterial peptide is a single-chain polypeptide coded by a gene of a rana nigromaculata amphibious animal in China and has molecular weight of 1495.85D and isoelectric point of 9.0; and complete sequence of the rana nigromaculata antibacterial peptide is valine-isoleucine-proline-isoleucine-valine-serin-glycine-leucine-leucine-serin-serin-leucine-leucine-glycine-lysine. A coding gene of the rana nigromaculata antibacterial peptide is composed of 333 nucleotides, wherein nucleotides No 142-186 are used for coding a mature part. The artificially synthesized rana nigromaculata antibacterial peptide has strong antibacterial action and high safety, also has the advantages of simple sequence and convenience for synthesis and can be applied to a novel antiinfection medicine.

Polyglycerol anti-microbial agents and compositions are provided. The agents are effective against a variety of pathogens including fungi, Gram positive bacteria and Gram negative bacteria yet are expected to have low human toxicity due in part to their polymeric nature. Applications for the polyglycerol anti-microbial agents and compositions include those involving human and plant contact, such as cosmetics, hair care products, textiles and plant protections, as well as in applications with much less human contact, such as plastics, coatings, wood, paper and other materials of construction.

The invention provides a method for preparing antibacterial peptide, which comprises the following steps: firstly, preparation of primary seeds; and secondly, solid fermentation. Because the method adopts the means of solid fermentation to prepare the antibacterial peptide, large-scale equipment investment is not required; the manufacturing technique is simple and convenient; acclimated engineering bacteria are easy to culture, propagate and produce; fermented products do not require separation and purification and can be directly applied; and the method is easy to promote. Moreover, the antibacterial peptide produced by the method has antibacterial activity with broad spectrum, and not only has strong killing effect on harmful bacteria such as golden yellow staphylococcus, mildew and so on and fungus but also has killing effect on viruses.

The inventiondiscloses an environment-friendly efficient binding agent for plywood. The binding agent is prepared from raw materials in parts by weight as follows: 5-7 parts of vinyl acetate, 0.1-0.5 parts of an emulsifier, 6-8 parts of water glass, 15-25 parts of polyvinyl alcohol, 0.3-0.7 parts of an anti-mildew bactericide, 10-20 parts of a thickening agent, 15-17 parts of a filling agent, 6-10 parts of cellulose ether, 10-20 parts of acrylic resin, 3-7 parts of starch and 40-60 parts of water. The efficient binding agent is higher in binding strength and water resistance, better in storage stability, free of toxic substances such as formaldehyde, benzene, toluene and the like and is completely non-toxic, harmless and environment-friendly, and besides, the efficient binding agent is short in curing time, long in effective service life and better in permeability. Meanwhile, a preparation method of the binding agent is lower in cost, adopts available raw materials and simple technology, is easy to operate, facilitates realization of industrial production, and has higher practical value and good application prospect.

The invention relates to an application of an isoflavonoids compound, that is, glabridin, to prepare an agricultural bactericide. The compound is used for inhibiting the following pathogenic funguses of crops: fusarium graminearum, colletotrichum gloeosporioides, botrytis cinerea, phytophthora infestans, or pyricularia grisea, and the following pathogenic bacteria of crops: erwina carotovora, pseudomonas solanacearum, ralstonia solanacearum, or pseudomonas syringae pv. actinidae. Furthermore, the compound can be prepared into various formulations with other agricultural auxiliaries, and the compound has a synergistic effect after combined with shikonin according to a certain weight ratio.

The present invention relates to one kind of extracellular antiseptic protein of Bacillus subtilis and its separation and purification process. The extracellular antiseptic protein has molecular weight of 41.9 kD, 26 mass-to-charge ratio peaks, and 5 peptide amino acid sequences including VTIVDEKGR, FSFSDVHNR, VYIADSTNFK, ELPISENLASVNMR and EAEWAYMITGK. Its separation and purification process includes the following steps: separating coarse protein from Bacillus subtilis Bs-916 via DEAE Sepharose F.F. separation, separating its B peak drips via Phenyl Sepharose 6 F.F. separation, separating the E peak drips via with hydroxyapatite column and obtaining the G peak drips as the purified extracellular antiseptic protein. The extracellular antiseptic protein has broad spectrum antiseptic activity, ribonuclease activity and agglutinin activity.

The present invention is directed to novel products, variants, pharmaceutically acceptable salts and prodrugs thereof, and medical use of such compounds for the treatment and / or management of sepsis, septicemia, septic shock, ocular infection, ocular inflammation, ocular angiogenesis, rheumatoid arthritis (RA), atherosclerosis, inflammatory bowel diseases (IBD), asthma, chronic obstructive pulmonary disease, fever syndromes, cachexia, psoriasis, autoimmune diseases, cardiac diseases, retinoblastoma, cancer and / or any disorder associated with inflammation, immunomodulation and microbial infection.

The invention discloses novel antimicrobial peptide Hainanenin-5 of Amolops hainanensis, a gene of the antimicrobial peptide Hainanenin-5 of Amolops hainanensis, a separation method and a chemical synthesis method for the antimicrobial peptide Hainanenin-5 of Amolops hainanensis and application of the gene of the antimicrobial peptide Hainanenin-5 of Amolops hainanensis, and belongs to the technical field of biomedicine. The Hainanenin-5 is small peptide which is obtained by the steps of electrically stimulating the Amolops hainanensis so as to collect a skin exudate, centrifuging, freeze-drying, performing gel filtration column chromatography and reverse phase-high performance liquid chromatography (RP-HPLC) and purifying, and has a C terminal containing a seven-membered ring; the molecular weight is 2,337.91; and the isoelectric point is 10.11. A primary structure of the Hainanenin-5 is Phe<1>a<2>Leu<3>Gly<4>Ala<5>Val<6>Thr<7>Lys<8>Arg<9>Leu<10>Pro<11>Ser<12>Leu<13>Phe<14>Cys<15>Leu<16>Ile<17>Thr<18>Arg<19>Lys<20>Cys<21>. A gene for encoding a Hainanenin-5 precursor consists of 321 nucleotides, wherein a mature peptide part is encoded by 139th to 201st nucleotides. The Hainanenin-5 has low molecular weight and a simple structure, only contains one disulfide bond, and can be conveniently prepared through chemical synthesis and genetic engineering. The Hainanenin-5 is used for preparing a therapeutic medicine for pathogenic microorganism infectious diseases, has broad-spectrum antimicrobial activity, has the activities on gram-positive bacteria, gram-negative bacteria and fungi (including clinically separated medicine-resistant strains), and also has the advantages of low hemolysis.

The invention discloses a process for producing feed antimicrobial peptides by bacillus subtilis and an application of the process for producing the feed antimicrobial peptides by the bacillus subtilis, and bacillus subtilis BW115 of which the collection number is CCTCC (China Center For Type Culture Collection) M 2016402. The process of the invention uses BW115 to express silkworm antimicrobial peptides, researches fed-batch high-density fermentation process, carries out multi-stage amplification culture to pilot production, and then comprehensively optimizes fermentation production process conditions, so that the biological potency of an obtained fermentation liquid reaches more than 10,000U / ml, the water contents of antimicrobial peptide products obtained through spray drying are smaller than 5%, and the biological potency of the antimicrobial peptide products reaches more than 80,000U / g. The antimicrobial peptides disclosed by the invention are applied to feed adding, so that production performances of bred animals can be obviously improved; compared with the existing similar product, the process for producing the feed antimicrobial peptides by the bacillus subtilis has the characteristics of high product biological potency, low cost of raw materials, short fermentation period, simple process and suitability for large-scale industrial production.

The invention discloses an application of chitosan guanidine cationic waterborne polyurethane in preparation of an antibacterial coating layer. A preparation method comprises the steps: taking polyester diol as a soft segment, diisocyanate as a hard segment and stannous octoate as a catalyst, and synthesizing an isocyanate end-capped prepolymer; carrying out primary chain extension reaction with small molecular chain extenders diethylene glycol and polyethylene glycol in an acetone dilution environment; introducing a cationic hydrophilic chain extender N-methyldiethanolamine, further carryingout chain extension, generating quaternary ammonium salt under the neutralization action of glacial acetic acid, and emulsifying the neutralized product by a chitosan guanidine aqueous solution underan ice-water bath condition to form the chitosan guanidine / cationic waterborne polyurethane emulsion; and coating a tetrafluoroethylene plate with a film by a film coater to obtain a chitosan guanidine / cationic waterborne polyurethane coating layer. The prepared polyurethane coating layer of chitosan guanidine has high transparency, excellent mechanical property, thermal stability, water resistance and adhesive force, so that the coating layer is expected to be used for antibiosis in the field with high requirements on weather resistance.

The invention provides an application of an antibacterial peptide as a preservative in preparing cosmetics. The antibacterial peptide provided by the invention has the beneficial effects that 1) the antibacterial peptide provided by the invention has broad-spectrum antibacterial activity and can effectively inhibit Gram negative bacteria, Gram positive bacteria, saccharomycetes and moulds; 2) the antibacterial peptide has good compatibility with raw materials of almost cosmetics, and the bacteriostatic ability is not affected by surfactants, proteins and Chinese herbal medicine additives in the cosmetics; and 3) the product does not contain a methanol releaser, highly irritant organic halogen components or chemical synthetic components, so that the product is high in safety and low in irritation.

The invention discloses a chiral cysteine nano self-assembled antibacterial material, a preparation method and application thereof, and belongs to the technical field of nano materials. According to the method, nano-gold is taken as a core, a small amount of sulfydryl polyethylene glycol is taken as a stabilizer, cysteine is fixed on the surface of the nano-gold through Au-S bonds in a fractional adding manner, and the chiral cysteine nano self-assembled antibacterial material is prepared. According to the method disclosed by the invention, the stability of nano-gold and simple cysteine can be remarkably improved, and the broad-spectrum antibacterial property on pathogenic bacteria such as escherichia coli and staphylococcus aureus is shown.

The present invention relates to one kind of extracellular antiseptic protein of Bacillus subtilis and its separation and purification process. The extracellular antiseptic protein has molecular weight of 41.9 kD, 26 mass-to-charge ratio peaks, and 5 peptide amino acid sequences including VTIVDEKGR, FSFSDVHNR, VYIADSTNFK, ELPISENLASVNMR and EAEWAYMITGK. Its separation and purification process includes the following steps: separating coarse protein from Bacillus subtilis Bs-916 via DEAE Sepharose F.F. separation, separating its B peak drips via Phenyl Sepharose 6 F.F. separation, separating the E peak drips via with hydroxyapatite column and obtaining the G peak drips as the purified extracellular antiseptic protein. The extracellular antiseptic protein has broad spectrum antiseptic activity, ribonuclease activity and agglutinin activity.

The invention discloses a 2-mercapto-1,3,4-thiadiazole derivative having a general formula I and a preparation method and application thereof. According to the method, meta-substituted phthalaldehyde reacts with 2-mercapto-5-amino-1,3,4-thiadiazole in ethanol so as to prepare a compound having the general formula I. The prepared 2-mercapto-1,3,4-thiadiazole derivative has broad-spectrum antibacterial activity, is capable of selectively recognizing dihydrogen phosphate ions, provides a critical basis for screening of lead compounds and has an application value in quantitative determination of dihydrogen phosphate ions in biological, environmental and medicinal samples and the like.

The invention relates to a new cationic antimicrobial peptide and application thereof, belonging to the technical field of biochemical polypeptide medicines. The new cationic antimicrobial peptide is a small molecule polypeptide which is designed artificially and synthesized chemically by taking a natural antimicrobial peptide in skin secretions of rana temporaria chensinensis david as a template. The small molecule polypeptide comprises 13 amino acid residues, and the C terminal of the small molecule polypeptide is amidated. The small molecule polypeptide has the sequence of IKKILSKIKKLLK-NH2, the molecular weight of 1552.13Da and the isoelectric point of 10.70, and the small molecule polypeptide has 6 positive charges. Tests prove that the small molecule polypeptide has broad-spectrum inhibiting and killing effects on a variety of bacteria and human cancer cells in vitro and has no hemolytic activity and eukaryotic cell toxicity in an antimicrobial and anticancer concentration range, and drug resistance is not easy to produce. In addition, experiments on animals show that the small molecule polypeptide has the effect of resisting wound infection. The small molecule polypeptide has small molecular weight, is synthesized artificially and conveniently, has extremely high application value and can be used for preparing new antibacterial, anti-inflammatory and anti-cancer medicines.

The invention relates to a formamide alkylbenzene-substituted sulfhydryl pyrrolidine carbapenem compound as shown in general formula (I), and also relates to the easy hydrolysis esters of the compound, the pharmaceutical acceptable non-toxic salts of the compound, the isomers of the compound, the hydrates of the compound, the hydrates of the esters or salts of the compound, the preparation methods for the compounds in formula (I), the purposes of the compounds as medicinal active substances and especially the applications of the compounds in the preparation of drugs for curing and / or preventing infectious diseases, as well as the drug combinations containing the compounds in formula (I). The detailed definitions of R1, R2, R3, R4, R5, R6 and n in the formula (I) are shown in the instruction book.

The invention relates to application of sugar, in particular to the application of algin oligosaccharide. The invention is characterized in that the algin oligosaccharide is used as a bacteriostat of germs or epiphyte. The invention proves that the bacteriostat can effectively inhibit the growing of Gram-negative bacteria or positive bacteria by bacteriostatic experiments, and shows broad spectrum antibiotic activity; the algin oligosaccharide is proved to have an antiinflammatory action and certain abirritation by acute inflammation experiments on animals.

The invention provides an extraction method for smakegourd active components, which includes: adding organic solvent or water into dry smakegourd fragment; extracting for 1-3 times, wherein time of each backflow extraction is 2-4 hours or time of normal temperature lixiviation is more than 24 hours, and added amount of organic solvent is 4-8mL per gram of smakegourd fragment; filtering; concentrating extract; and freeze-drying to obtain smakegourd extract. The organic solvent is one or more of methanol, acetone and ethyl acetate. The invention has advantages of easy operation, low cost, high extraction efficiency, high biological activity for the obtained extract, good fresh-keeping effect for fresh meat, fruit and vegetable, and better application prospect.

Antimicrobial / pharmaceutical preparations (e.g., solutions, gels, ointments, creams, sustained release preparations, etc.) which comprise chlorite (e.g., a metal salt of a chlorite) in combination with a peroxy compound (e.g., hydrogen peroxide), and methods for using such preparations for disinfection of articles or surfaces (e.g., contact lenses, counter tops, etc.), antisepsis of skin or other body parts, prevention or deterrence of scar formation and / or treatment and prophylaxis of dermal (i.e., skin or mucous membrane) disorders (e.g., wounds, burns, infections, cold sores, ulcerations, psoriasis, acne, or other scar-forming lesions).

The present invention relates to antimicrobial alkaline compositions suitable for use in medical and dental treatment. The compositions comprise a polyalkylene glycol or C3-C6 polyol compound and calcium hydroxide or components that generate calcium hydroxide in situ. The use of the compositions as dental medicaments, obturants, liners, oral disinfectants and bone cements is also described.