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Extraction method of Mongolian snakegourd effective ingredient

The technology of an active ingredient and an extraction method, which is applied in the field of extraction of the active ingredient of Gualou, can solve the problems of limited extraction efficiency, low extract activity and high equipment requirements, and achieves the effects of low cost, high extraction efficiency and high biological activity.

Inactive Publication Date: 2009-06-10
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Existing methods for extracting effective components of Trichosanthes include water extraction, water extraction and alcohol precipitation, supercritical CO 2 Extraction and ultrasonic extraction methods, etc. Among them, water extraction and water extraction and alcohol precipitation methods are simple, but the extraction efficiency is limited and the activity of the extract is low; supercritical CO 2 Although the extraction and ultrasonic extraction methods have improved the extraction efficiency to a certain extent, the operation is complicated, the equipment requirements are high, and the cost is high.

Method used

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  • Extraction method of Mongolian snakegourd effective ingredient
  • Extraction method of Mongolian snakegourd effective ingredient
  • Extraction method of Mongolian snakegourd effective ingredient

Examples

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Effect test

Embodiment 1

[0017] Embodiment 1: Fructus Trichosanthes water extract extracts (comparative example)

[0018] Take 1000g of dried melon skin, pulverize, sieve, put in the extraction device and add 6L of water, extract for 24 hours, filter and centrifuge to obtain the extract, the extract is freeze-dried to obtain a solid extract, low temperature (4°C) Save for later.

Embodiment 2

[0019] Embodiment 2: Trichosanthes quince ethyl acetate extract extract

[0020] Take 1000g of dried melon skin, crush and sieve, add 4L (single dose) of petroleum ether to degrease 3 times, 5 hours each time, temperature 30-40°C, filter, evaporate the filter residue to dry petroleum ether, add 5L (single dose) times) of ethyl acetate at room temperature and extracted 3 times at room temperature for 24 hours each time. The extracts were combined, concentrated under reduced pressure, and freeze-dried to obtain the ethyl acetate extract of Trichosanthes melaka, which was stored at 4°C for future use.

Embodiment 3

[0021] Embodiment 3: the preparation of trichosanthes petroleum ether extract

[0022] Take 1000g of dried melon skin, pulverize and sieve, add 4L (single dose) of petroleum ether to degrease 3 times, each time for 5 hours, at a temperature of 50-60°C, filter, evaporate the filter residue to dry petroleum ether, add 5L (single dose) Subsequent use) of methanol heating and reflux extraction 3 times, 2 hours each time, combined extracts, concentrated under reduced pressure until the volume is 1 / 5 of the original volume, then extracted with petroleum ether, concentrated under reduced pressure, freeze-dried, that is Trichosanthes petroleum ether extract, stored at 4°C for later use.

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Abstract

The invention provides an extraction method for smakegourd active components, which includes: adding organic solvent or water into dry smakegourd fragment; extracting for 1-3 times, wherein time of each backflow extraction is 2-4 hours or time of normal temperature lixiviation is more than 24 hours, and added amount of organic solvent is 4-8mL per gram of smakegourd fragment; filtering; concentrating extract; and freeze-drying to obtain smakegourd extract. The organic solvent is one or more of methanol, acetone and ethyl acetate. The invention has advantages of easy operation, low cost, high extraction efficiency, high biological activity for the obtained extract, good fresh-keeping effect for fresh meat, fruit and vegetable, and better application prospect.

Description

(1) Technical field [0001] The invention relates to a method for extracting effective components of Trichosanthes trichosanthes, in particular to an extraction method for active components with bactericidal and fresh-keeping functions. (2) Background technology [0002] Melon basket is a perennial climbing herb of Cucurbitaceae, also known as Trichosanthes, Dugua, Yaogua, Diaogua. The fruits, seeds and tubers are all used for medicinal purposes. China is distributed in Hebei, Henan, Shandong, Shanxi, Jiangsu, Anhui, Zhejiang, Shaanxi, Gansu and other provinces; it is also produced in Korea and Japan. Both wild and cultivated. Stems up to 10 meters long, much branched. Root tubers are thick, long cylindrical, rich in starch. Leaves alternate, nearly round or nearly heart-shaped, often 5-7 palmately lobed to deeply lobed. Flowering in summer and autumn, white corolla, unisexual. Male flowers form racemes, female flowers are solitary in leaf axils; corolla 5-lobed, lobes ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/28A23L1/29A23L1/212A23L3/3472A23L33/105
Inventor 许培雅黄海婵石圆圆
Owner ZHEJIANG UNIV OF TECH
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