57 results about "Rana" patented technology

The present invention relates to immunotoxins, that effectively kill malignant cells having a given surface marker and nucleic acid constructs encoding them. These reagents comprise a toxic moiety that is derived from a Rana pipiens protein having ribonucleolytic activity linked to an antibody capable of specific binding with a chosen tumor cell.

The invention discloses a method for extracting RNA of sweet potato root tuber and application thereof. Beta-mercaptoethanol and polyvinylpyrrolidone are used to prevent polyphenols from interfering the separation of RNA; and in addition, the NaCl with high concentration, absolute ethyl alcohol and potassium acetate are used, so the polysaccharose can be rapidly precipitated, and the influence of the polysaccharose on the extraction of RNA can be prevented. When preventing the interference effect of the polyphenols and the polysaccharose on the separation of RNA, RANA can still be remained in the solution, so the RNA with good quality, high purity and good completeness can be obtained, and the attainment rate and the yield of the RNA are large. The method has high practical application value, and can satisfy the research of molecular biology such as reverse transcription, Northern hybridization, cDNA library construction, transcriptome analysis and the like.

A defatted frog ovum powder as female health-care food for preventing osteoporosis in female menopausal phase and treating climacteric syndrome is prepared from defatted frog ovum powder, soybean extract, calcium, VC and additive.

The invention relates to a zymolysis method for extracting an oligopeptide mixture with high F value by Rana temporaria eggs. The method has the steps as follows: (1) after are compressing dried Rana temporaria eggs, and egg oil is filtered to obtain egg residue for standby; (2) the egg residue is placed in a reaction kettle and purified water is added, stirred and heated to 48 DEG C to 52 DEG C; and then after pH value is regulated to 8.0 to 8.5, peptide compound enzyme with the weight to be 1 percent to 3 percent of that of the egg residue is added for hydrolysis for 4h to 6h; the temperature is heated up to 80 DEG C to 85 DEG C, and insulated for 10min to 20min; and filtering is carried out to obtain filtrate which is frozen, dried and shattered to obtain the oligopeptide mixture with high F value. Or step (2) can be as follows: pH value is regulated to 1.5 to 2.5, pepsin with the weight to be 1 percent to 3 percent of the egg residue is added for hydrolysis for 4h to 6h; and after the pH value is regulated to 6 to 7, actin enzyme with the weight to be 0.5 percent to 1.5 percent of that of the egg residue is added for hydrolysis for 5h to 7h; and then the temperature is raised to 85 DEG C to 95 DEG C till finally the oligopeptide mixture with high F value is obtained. The method is characterized by reasonable technique, high yield and recyclable egg residue waste.

The present invention provides a lygodium japonicum (thunb.) sw lead-removing health beverage and a preparation method thereof, wherein the raw material components comprise, by weight, 5-8 parts of ginseng, 3-4 parts of milkvetch root, 4-5 parts of lotus seeds, 4-5 parts of longan, 4-5 parts of lily, 6-8 parts of lygodium japonicum (thunb.) sw, 1-2 parts of silene conoidea l, 10-15 parts of beta vulgaris leaf, 3-4 parts of celery leaf, 3-4 parts of white radish leaf, 3-4 parts of lettuce leaf, 0.1-0.2 part of rana temporaria oil, 4-6 parts of a nutritional additive, 0.1-0.3 part of citric acid, 5-8 parts of honey, 2-3 parts of xylitol, 0.1-0.3 part of a lotus leaf extract, and 800-1000 parts of water. The lygodium japonicum (thunb.) sw lead-removing health beverage has a lead-removing effect, has a sweet and sour taste, is particularly suitable for long-term use of high blood lead people, and has promotion application values.

The invention discloses an environment-friendly type feed additive capable of preventing skeletal muscle edema of Rana catesbeiana Shaw. The feed additive comprises sodium selenite, vitamin E, vitamin C, folium isatidis and astragalus membranaceus. The environment-friendly type feed additive has a useful effect of promoting growth of the Rana catesbeiana Shaw, has a remarkable effect of preventing the skeletal muscle edema of legs of the Rana catesbeiana Shaw, has a useful effect of treating the skeletal muscle edema of the Rana catesbeiana Shaw fed with changed feeds and is capable of lowering the probability of inducing the death of the Rana catesbeiana Shaw by the skeletal muscle edema of legs.

The invention relates to an antibacterial lotion containing rana antibacterial peptides and a preparation process thereof. The rana antibacterial peptides are dissolved in a phosphate buffer with the pH of between 5 and 7 and are added with a mannitol protecting agent to prepare a light yellow transparent liquid, namely the antibacterial lotion. The lotion has the functions of sterilization, disinfection, itching relief, astringency and hemostasis, fever removal and insecticide, and the like, is non-toxic and harmless to a human body, does not irritate skin and mucous membrane, can be widely applied to sanitation and cleanness of human skin and mucous membrane, antibacterial and sterilization and other fields with sterilizing effect reaching 100 percent. Furthermore, due to the addition of the mannitol protecting agent, the duration of effective antibiotic activity of the antibacterial lotion is greatly prolonged.

The present invention relates to rana grahami antibiotic peptide and its application, and belongs to the field of biomedicine. Rana grahami antibiotic peptide is one kind of cyclic polypeptide separated from the secretion of amphibian rana grahami, and has molecular weight of 1705.3 dalton, isoelectric point of 9.39, and primary structure of polypeptide sequence Leu Lys Gly Cys Trp The Lys Ser Ile Pro Pro Lys Pro Cys Phe(LKGCWTKSIPPKPCF) with cysteines in the 4-th and 14-th forming intramolecular disulfide bond. Its preparation process includes collecting secretion of ether stimulated rana grahami, centrifuging to eliminate precipitate, freeze drying, chromatographic separation in filtering gel column and separating purification in reverse high pressure liquid chromatogram. The rana grahami antibiotic peptide has powerful effect of inhibiting bacteria, fungi and viruses and no hemolytic activity, and may be used in preparing medicine for treating pathogenic microbe infection diseases.

The invention discloses a high-yield disease-resistant Rana rugulosa breeding method. The method includes feed formulation, feed preparation and breeding management of Rana rugulosa. The feed preparation comprises, by weight, 170-180 parts of soybean residue, 65-67 parts of oat bran, 54-58 parts of Nereid powder, 14-17 parts of sesame residue, 0.4-0.5 part of lactic acid bacteria starter, 24-26 parts of minced fillet, 3-4 parts of banana vinegar, 42-45 parts of viviparous meat powder, 4-5 parts of chilli paste, 2-3 parts of peanut oil, 12-15 parts of small white shrimp, 15-17 parts of celery stem and leaf powder, 12-14 parts of nori powder, 23-25 parts of pawpaw powder, 31-34 parts of black glutinous rice, 7-8 parts of white sesame, 27-29 parts of red bean, 4-5 parts of red sauce, 1-2 parts of Cortex eucommia, 2-3 parts of Radix platycodi, 1-2 parts of diatomite and 2-3parts of fish bone meal. The feed formulation is high in intake inducing performance and disease resistance, and Rana rugulosa is active in intake, quick in growing, early in metamorphosis time, large in body, tender and delicious in meat quality and rich in nutrition.

Disclosed is an pharmacy application of both a serine protease inhibitor of Rana grahami Graham's frog and a gene thereof, pertaining to biomedicine field. Serine protease inhibitor of Rana grahami Graham`s frog is a cyclic polypeptide encoded by a gene of Rana grahami Graham's frog, which is a Chinese amphibian. Said serine protease inhibitor has a molecular weight of 1951.38 dolton, an isoelectric point of 9.51, and no enzymatic activity. The complete sequence thereof is NH2-AVNIPFKVHFRCKAAFC-COOH,in which Cys at 12th site and Cys at 17th site form an intramolecularly disulfide bond. The coding gene is composed of 301 nucleotides, and the mature coding part is nucleotides from 160th site to 213th site. Artificial serine protease inhibitor of Rana grahami Graham's frog has strong suppression activity for serine protease, and can be used in pharmacy for preparing medicine for tumor, gastritis, and pancreatitis. Said serine protease inhibitor has a simple sequence and can be synthesized with ease.

The invention discloses a non-finger disc odor-frog antibiotic peptide and making method in the biochemical and biological medical domain, which comprises the following steps: stimulating the active non-finger disc odor frog through electricity; collecting secretion; centrifuging; removing sediment; freezing; drying; proceeding ion exchange; gelling; filtering; proceeding reverse-phase chromatographic separation to purify; obtaining the peptide named as OdorgrinA with 33 amino acids, theoretical molecular weight at 3365. 81D, real molecular weight at 3365. 24D, theoretical isoelectric point (pI) at 9. 31 and whole sequent structure at NH2-GLLDTFKNLALNAAKSAGVSVLNSLSCKLSKTC-COOH. The invention possesses broad spectrum activity, which can used to produce antibiotic drug and antibiotic agent.

The invention discloses a PCR (Polymerase Chain Reaction) rapid assay kit for rana boulenger guenther-infecting staphylococcus aureus and an assay method. The PCR rapid assay kit comprises a PCR assay kit for assaying the staphylococcus aureus of ana boulenger guenthers, which includes 2X mixed reaction buffer, sense primer S-F:5minute -AGGTAACGGCGTAAATAG-3minute, antisense primer S-R: 5minute-ACTTGCTTCAGGACCATA-3minute, Taq enzyme and sterilized ddH2O. The PCR rapid assay kit disclosed by the invention has the advantages of rapidness, accuracy and specificity in the assay of the rana boulenger guenther-infecting staphylococcus aureus, meets the requirement of clinical diagnosis and provides convenient conditions for the assay of the staphylococcus aureus infecting the rana boulenger guenthers.

The invention discloses a non-finger disc odor-frog antibiotic peptide and making method in the biochemical and biological medical domain, which comprises the following steps: stimulating the active non-finger disc odor frog through electricity; collecting secretion; centrifuging; removing sediment; freezing; drying; proceeding ion exchange; gelling; filtering; proceeding reverse-phase chromatographic separation to purify; obtaining the peptide named as OdorgrinA with 33 amino acids, theoretical molecular weight at 3365. 81D, real molecular weight at 3365. 24D, theoretical isoelectric point (pI) at 9. 31 and whole sequent structure at NH2-GLLDTFKNLALNAAKSAGVSVLNSLSCKLSKTC-COOH. The invention possesses broad spectrum activity, which can used to produce antibiotic drug and antibiotic agent.

The invention discloses a novel antioxidant active polypeptide OA-VI12 and a preparation method and an application thereof. An amino acid sequence of the novel antioxidant active polypeptide OA-VI12 is VIPFLACRPLGL. The antioxidant active polypeptide is extracted from skin secretions of rana andersonli, and the polypeptide has the characteristics of natural source, high activity and the like. Thenovel antioxidant active polypeptide OA-VI12 obtained by purification shows the relatively high stability and antioxidant activity and plays a beneficial role in protecting cells from UV irradiation and oxidative stress caused by hydrogen peroxide stimulation, and more importantly, the novel antioxidant active polypeptide OA-VI12 also achieves the effect of protecting the skin from photodamage caused by ultraviolet radiation, so that it is indicated that the novel antioxidant active polypeptide OA-VI12 has a relatively wide medical application prospect. The novel antioxidant active polypeptideOA-VI12 has the beneficial characteristic of being capable of serving as an anti-ultraviolet drug candidate.

The invention discloses a PCR detection kit and a detection method of rana boulengeri-infected shewanella putrefaciens, wherein the detection kit consists of 1.0mL of a double-reaction mixed buffer solution; forward and reverse primers according to an optimized design; Taq DNA polymerase; a positive control solution; a negative control solution; and ddH2O. The detection kit disclosed by the invention can overcome the shortcomings of an existing shewanella putrefaciens detection method; and the rana boulengeri shewanella putrefaciens detection kit disclosed by the invention has the advantages of being rapid, accurate and specific in clinical field, so as to facilitate the detection of rana boulengeri-infected shewanella putrefaciens.

The invention discloses a non-finger frog skin peptide and gene and application in the drug in the biological medical domain, which codes a single-chained peptide with molecular weight at 3355.95 and isoelectric point at 10.14, wherein the primary structure of peptide is Gly Leu Gly Gly Ala Lys Lys Asn Phe Ile Ile Ala Ala Asn Lys Thr Ala Pro Gln Ser Val Lys Lys Thr Phe Ser Cys Lys Leu Tyr Asn Gly; the gene of secretory peptide is composed of 731 nucleic acids, which codes 520-616th nucleic acid; the peptide possesses obvious bacteria and fungi inhibiting action, which can be applied to make microbe infection disease drug.

The invention relates to the field of agricultural breeding, and discloses an integrated overwintering breeding method for hatching and breeding rana chinensis in the same pond. The method comprises the steps of (1) greenhouse construction, (2) hatching pond construction, (3) construction of an anti-escaping fence area and a rana chinensis feeding activity area, (4) spawn hatching and froglet breeding, (5) overwintering management, (6) management in early spring and (7) management of a second breeding period. According to the method, the management is convenient, the labor is saved, and each person can manage ten greenhouses; the survival rate of froglets reaches 90% or above in the overwintering process; the rana chinensis can grow quickly, the breeding period is short, from spawn hatching to a fattening period, the rana chinensis experiences overwintering once, in autumn of the next year, adult rana chinensis can be sold, and the economic benefit is high.

The invention relates to the technical field of rana spinosa culture, in particular to a heat preservation culture technology of rana spinosa. A hatching pool is designed into a flat bottom type, a dripping device is arranged at the top, an aeration facility is laid at the bottom, a culture pool is designed into a gentle slope type, the ratio of a water area to the gentle slope and land is 1:1:1,the gradient of the gentle slope is 30%, a water outlet and an aeration port are formed in the water area part, water temperature is controlled by a coal-fired boiler, hot water at about 50 DEG C is boiled in the boiler to enter a buffer pool, the hot water is mixed with cold water, and the temperature of water in the culture pool is kept at about 24 DEG C stably. By control over air temperature and water temperature of a culture room, the metamorphosis period of tadpoles is shortened and the metamorphosis rate is increased; hibernation habits of the rana spinosa are changed, the rana spinosadoes not enter the hibernation period and can take food and grow all the year round, the three-year delivery period of the rana spinosa is shortened to two years, and 1 / 3 of feeding period is reduced;through artificial adjustment and control of temperature and humidity of a bait breeding area and a reproduction area, the most proper temperature for adult reproduction is guaranteed, and yield maximization is realized.

The invention discloses convenient and pollution-free rana boulenger guenther tadpole matched bait capable of achieving fast growth. The convenient and pollution-free rana boulenger guenther tadpole matched bait capable of achieving fast growth is characterized by being prepared from, by weight, 23% of soybean meal, 13% of shelled eggs, 21% of chub meat, 15% of corn flour, 26% of cabbages and 2% of yeast powder. The convenient and pollution-free rana boulenger guenther tadpole matched bait capable of achieving fast growth has the advantages that the raw materials are easy to obtain, a preparation method is simple, a feeding method is convenient and fast to use, tadpoles grow fast, and pollution is avoided, and the bait is suitable for feeding the tadpoles in the prometaphase of rana boulenger guenther.

The invention relates to a staged feeding and female mutagenesis multifunctional feed of rana tadpole for reducing water body pollution. The feed is characterized in that: according to an adopted technical formula, the feed consists of a main material and auxiliary materials; and formulas of per kilogram of feed at different stages are different. The feed has the advantages that: a produced tadpole bait is in a frozen state at normal temperature, and is difficult to dissolve in water when fed; bait waste is reduced; the pollution of bait residues to a water body of a culture pond is avoided; the feed is similar to rana egg jelly and has prominent palatability; nutrients are proportioned according to different growth stages, and the feed has comprehensive and rich nutrition; the feed has obvious effects of preventing, controlling and treating the common diseases of the tadpole when a Chinese herbal medicine compound preparation for preventing the diseases of the tadpole is added, does not have toxic or side effect or form residues in the body of the tadpole after being used, and prevents germs from medicament resistance and medicament tolerance; when a rana tadpole gender inducer is added into the feed according to a scientific proportion, malformed frogs are avoided while the proportion of female frogs is greatly improved; the bait waste caused by dissolution and sedimentation of the feed is reduced; accurate control over the feeding amount of the feed is realized; and the culturing cost is greatly reduced.

The invention provides a polyculture method of dendrobium officinale and rana spinosa. According to the polyculture method, rana-spinosa inhabiting ponds are arranged between adjacent seed beds, a plurality of pavement rana-spinosa sunning areas are arranged at equal intervals between adjacent rana-spinosa inhabiting ponds and between adjacent seed beds, the movement range of rana spinosa is limited in the rana-spinosa inhabiting ponds and in the seed beds, rana spinose preys on natural enemies when dendrobium officinale is planted, excreta of rana spinose is used as growth fertilizer of dendrobium officinale, and the mutualism and polyculture between dendrobium officinale and rana spinosa are achieved. By the adoption of the polyculture method of dendrobium officinale and rana spinosa, the land is saved, and the utilization efficiency of greenhouses is improved; the insect damage of dendrobium officinale is reduced, the superb fertilizer is provided for the growth of dendrobium officinale, and the production cost is reduced; an optimal growth environment is provided for rana spinosa, palatable bait is provided, the meat quality of rana spinosa is improved, and the production cost is reduced; cyclic planting and culturing are conducted, the purpose of zero emission is basically achieved, and the polyculture method of dendrobium officinale and rana spinosa is environmentally friendly; dendrobium officinale and rana spinosa belong to health-preservation nourishing products, the purpose of two-way income is achieved, and the economic benefits are significant.

A soft capsule of frog ovum fat for preventing arteriosclerosis and hyperlipomia, and whitening and protecting skin is prepared from frog ovum fat, plant embryo bud oil and VE.

The invention relates to rana tiglina feed. The feed comprises components in parts by weight as follows: 6-10 parts of imported fish meal, 10-12.5 parts of chicken meal, 7.7-20.5 parts of soybean meal, 13-23 parts of fermented soybean meal, 20-21 parts of flour, 5-6 parts of wheat, 2-6 parts of corn protein powder, 5-7 parts of soy protein concentrate, 1-1.5 parts of squid paste, 1-2 parts of soybean phospholipid oil, 1-2 parts of soybean oil, 1.5-2 parts of fish oil, 2-3 parts of monocalcium phosphate, 0.2-0.3 part of choline chloride, 1-1.5 parts of vitamin premixed feed, 1-1.5 parts of mineral premixed feed, 0.1-0.3 part of lysine, 0.2-0.3 part of methionine and 0.01-0.02 part of an antioxidant. By matching of various nutrient substances, the use amount of the fish meal is substantiallyreduced and the nutrient requirement of rana tiglina is met, so that the rana tiglina feed is efficient and environmentally friendly.

A domestication method of a full-feed black-spotted frog species. In the first year, the eggs of the black-spotted frog are cultivated in a simulated wild environment and fed with live bait; in the second year, after the young frogs of the black-spotted frog complete metamorphosis and land , feed with live bait for one month, after that, gradually reduce the amount of live bait, add feed to the live bait for feeding, and gradually increase the proportion of feed until all feed is used for feeding; in the third year, in the black After the young frogs of spotted frogs complete their metamorphosis and come ashore, they are directly fed with feed, and the young frogs will have adverse reactions. After feeding with feed for one month, some young frogs are eliminated, and some young frogs are used to feeding with feed; in the fourth year, Black-spotted frogs are raised in the frog pond and fed with feed. The invention subverts the tradition, reduces the production cost, liberates a large amount of labor force, is suitable for large-scale industrial production, greatly shortens the growth cycle, greatly improves the output, the yield per mu reaches more than 3,000 jin, and the economic benefit is significantly increased.

The invention discloses a non-finger frog secretory peptide and gene and application in the drug in the biological medical domain, which codes a cyclic peptide with molecular weight at 1587.98 and isoelectric point at 9.7, wherein the primary structure of peptide is Phe Met Pro Ile Leu Ser Cys Ser Arg Phe Lys Arg Cys with disulfide bond in the seventh cysteine and thirteenth cysteine; the gene of secretory peptide is composed of 300 nucleic acids, which codes 138-177th nucleic acid; the peptide possesses obvious bacteria and fungi inhibiting action, which can be applied to make microbe infection disease drug.

The invention discloses a method for breeding rana catesbiana shaw. The method specifically comprises the following steps that step 1, a place close to a water resource is selected to carry out digging of a culture pond, the cross section of the culture pond is inverted trapezoidal, iron gauze is arranged on the edge of the culture pond, the height of the iron gauze is 1-1.5 meters; step 2, beforeyoung rana catesbiana shaw is put, firstly, glacial acetic acid is poured into the culture pond to disinfect the culture pond; step 3, after disinfection is completed, water containing the glacial acetic acid in the culture pond is discharged, then new water is introduced and then discharged after soaking is carried out for three days, new water is introduced again, and the step is repeated threetimes. The method for breeding rana catesbiana shaw has the advantages that feed made by mixing tenebrio molitor living bodies, maggot living bodies and animal giblets is fed, 60-70% of feed is evenly spread on a dry edge zone in the culture pond, thereby increasing the exercise amount of rana catesbiana shaw, so that the meat of rana catesbiana shaw becomes more compact, and the taste of rana catesbiana shaw is ensured.

The invention discloses a young frog management technology for wood frogs. The management technology is characterized in that bleaching powder of 1 ppm is used for disinfection of whole fences beforeyoung frogs enter a frog house; the young frogs are placed into the frog house according to the density of 300-400 frogs in each square meter of the frog house; water is sprayed once every day, and the amount of the water is not too much so that accumulated water on the ground is prevented; feeding is carried out from 4 a.m. to 5 a.m. in the morning or from 5 p.m. to 6 p.m. in the afternoon everyday; each young frog is fed by using 1-2 tenebrio molitors of 2-3 instars every day; after 40 days, feeding is carried out twice every day by using 2-3 tenebrio molitors of 4-5 instars; and corn stalks or tree branches and leaves and the like are placed on the periphery of the frog house, so that the landing of the young frogs are facilitated. The management technology solves the problem that whenwood frogs are bred in coastal regions, the survival rate of the young frogs is low because of the climate environment, so that the management technology can be widely adaptable to coastal regions.

Provided is an adult frog management technology for wood frog breeding. The adult frog management technology for wood frog breeding is characterized by including the steps that adult frogs are released according to the density of 40-50 per square meter of a frog pen, larvae of tenebrio molitor are released as main feed, feeding is conducted once every day at 4-7 AM or 5-7 PM, and each wood frog isfed with 3-4 larvae each time. By means of the adult frog management technology for wood frog breeding, the problem is solved that growth is slow in frigid regions, so that the social requirements are not met; the adult frog management technology for wood frog breeding is widely suitable for being used in coastal regions.