Enzymatic hydrolysis method for extracting high F value oligopeptide mixture from rana chensinensis egg oil

A mixture and oligopeptide technology, which is applied in the field of enzymatic hydrolysis for extracting high F-value oligopeptide mixture from Rana sylvatica eggs, can solve problems such as low yield, and achieve the effects of high yield, reasonable process and improved yield.

Inactive Publication Date: 2009-03-25
TONGHUA CITY INST OF FOREST FROG
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AI Technical Summary

Problems solved by technology

It is common to use corn and soybeans as raw materials to extract high F-value o

Method used

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Examples

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Embodiment 1

[0010] Enzymolysis method for extracting high F-value oligopeptide mixture from Rana roe eggs: take 5 kg of Rana ova eggs and squeeze them through a screw press to obtain 4.55 kg of egg dregs, put them in an enamel reaction kettle, add 147.1 kg of purified water, stir evenly, and heat 50°C, use 0.5mol / L NaOH to adjust the pH value to 8.35, add 0.1kg of short peptide compound enzyme, hydrolyze for 5 hours, then warm to 83°C, keep for 15 minutes, filter, take the filtrate to freeze-dry, crush to obtain high F-value oligopeptide mixture, 2.8 kg of high-F-value oligopeptide mixture extracted from Rana ova. The measured F value is 20.16.

Embodiment 2

[0012] Enzymolysis method for extracting high F-value oligopeptide mixture from Rana roe eggs: take 5 kg of Rana ova eggs and squeeze them through a screw press to obtain 4.55 kg of egg dregs, put them in an enamel reaction kettle, add 147.1 kg of purified water, stir evenly, and heat To 37°C, adjust the pH value to 2 with 0.5mol / L HCl, add 0.091 kg of pepsin, hydrolyze for 5 hours, adjust the pH value to 6.5 with 0.5mol / L NaOH again, add 0.023 kg of actinase, and hydrolyze for 6 hours Heat to 90°C, keep warm for 12 minutes, filter, freeze-dry the filtrate, and pulverize to obtain 2.75 kg of high F value oligopeptide mixture. The measured F value is 20.18.

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Abstract

The invention relates to a zymolysis method for extracting an oligopeptide mixture with high F value by Rana temporaria eggs. The method has the steps as follows: (1) after are compressing dried Rana temporaria eggs, and egg oil is filtered to obtain egg residue for standby; (2) the egg residue is placed in a reaction kettle and purified water is added, stirred and heated to 48 DEG C to 52 DEG C; and then after pH value is regulated to 8.0 to 8.5, peptide compound enzyme with the weight to be 1 percent to 3 percent of that of the egg residue is added for hydrolysis for 4h to 6h; the temperature is heated up to 80 DEG C to 85 DEG C, and insulated for 10min to 20min; and filtering is carried out to obtain filtrate which is frozen, dried and shattered to obtain the oligopeptide mixture with high F value. Or step (2) can be as follows: pH value is regulated to 1.5 to 2.5, pepsin with the weight to be 1 percent to 3 percent of the egg residue is added for hydrolysis for 4h to 6h; and after the pH value is regulated to 6 to 7, actin enzyme with the weight to be 0.5 percent to 1.5 percent of that of the egg residue is added for hydrolysis for 5h to 7h; and then the temperature is raised to 85 DEG C to 95 DEG C till finally the oligopeptide mixture with high F value is obtained. The method is characterized by reasonable technique, high yield and recyclable egg residue waste.

Description

technical field [0001] The invention relates to a health product processing method, that is, an enzymatic hydrolysis method for extracting a high F-value oligopeptide mixture from Rana roga eggs. Background technique [0002] In the prior art, a high F value oligopeptide (High F Value Oligopeptide) is a low-molecular-weight active peptide formed after proteases act on proteins. Fischer value, referred to as F value, is the molar ratio of branched chain amino acids (BC) to aromatic amino acids (AC). It has good flavor and rich nutrition and is easily digested and absorbed by the human body, and has the functions of preventing and treating hepatic encephalopathy, improving protein nutritional status, anti-fatigue and easy absorption, etc., and has attracted much attention in recent years. It is common to use corn and soybeans as raw materials to extract high F-value oligopeptide mixtures. The disadvantage is that the yield is low, only about 20%. The egg residue after extrac...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 吕集杜景新翟丽红王丽燕俊青曲淑杰杨晓姝郑旭
Owner TONGHUA CITY INST OF FOREST FROG
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