50 results about "Rana chensinensis" patented technology

A pichia pastoris genetic engineering hybrid antibacterial peptide CC29 and a preparing method thereof are disclosed. Musca domestica cecropin obtained by cloning in a laboratory and rana chensinensis Chensirin are adopted as parent peptides and are subjected to hybrid designing to obtain the novel hybrid peptide with low hemolysis and high antibacterial activity and a fermentation strain capable of efficiently expressing the novel hybrid peptide. Compared with natural peptides, the peptide has huge advantages. A foundation of large-scale production of the antibacterial peptide is laid.

The invention provides minimally invasive repair gel with rana chensinensis skin polypeptide. The minimally invasive repair gel is composed of, by mass, 100 parts of rana chensinensis skin polypeptide, 65 parts of acrylic acid(ester) / C10-30 alkanol acrylic ester cross-linked polymer, 200 parts of trehalose, 5 parts of ethylene diamine tetraacetic acid, 300 parts of glycerinum, 10 parts of carboxymethyl beta-dextran sodium, 300 parts of butanediol, 70 parts of 1,2-hexanediol, 25 parts of phenoxyethanol, 15 parts of PEG-40 hydrogenated castor oil, 100 parts of hydrolyzed royal jelly protein, 26 parts of palmitoyl tripeptide-5 and 4 parts of sodium hyaluronate. Rana chensinensis skin polypeptide serves as a main component, thickening agents, wetting agents, skin conditioning agents, chelating agents, solubilizing agents and the like serve as auxiliary components, and then the gel with a bacterium restraining function and a wound repairing function is prepared. Rana chensinensis skin polypeptide is prepared through a neutral protease K enzymolysis method, and therefore the polypeptide molecular weight can be smaller than 2000 Da, and the polypeptide can be more easily absorbed by human skin; meanwhile, the pH value of the prepared gel finished product ranges from 5.5 to 7.5, and the stimulus to skin is avoided.

The invention relates to a preparation method of rana chensinensis frogspawn calcium amino acid chelated food, belonging to the agricultural product processing technique. The preparation method comprises the following steps: taking frogspawns as raw materials, drying, powdering, extracting and degreasing to obtain purified degreased frogspawn powder; under mild conditions, preparing frogspawn amino acid compound hydrolyzed solution by enzymolysis of various proteolytic enzymes; and then taking calcium chloride as a calcium source to prepare rana chensinensis frogspawn compound calcium amino acid chelated food by the chelation. In the method, waste frogspawns with abundant nutrition are prepared into healthcare food so as to spread the application range of the frogspawn. The method has thecharacteristics of reasonable and simple process and high utilization ratio of the frogspawns.

A Ca-enriched tablet of tree frog bone contains tree frog bone powder, granular white sugar, glucose and milk powder proportionally. It is prepared from tree frog bone through crushing, mixing it with distilled water, enzymolyzing, low-temp vacuum drying, pulverizing, mixing it with granular white sugar, glucose and milk powder, and tabletting.

The invention belongs to the field of medicines and health care, and in particular relates to a rana chensinensis egg oil health care product and a preparation method thereof. The rana chensinensis egg oil health care product contains rana chensinensis egg oil which is extracted from rana chensinensis eggs by CO2 supercritical extraction technology and has health care effect; and the rana chensinensis egg oil is distilled by molecular distillation technology to form a molecular distillation product of the rana chensinensis egg oil. The peculiar smell of the rana chensinensis egg oil is effectively eliminated, the edible mouthfeel is improved, and the quality guarantee period of the health care product is effectively prolonged; and effective components are concentrated in the extraction process, the content of the effective components of extract is improved, and the quality of the heal care product is improved. Meanwhile, isoflavone-containing soyabean extract, vitamins and calcium are added to the rana chensinensis egg oil health care product so as to improve the health care effect.

The invention relates to an antimicrobial peptide separated from the skin of the northeast Rana chensinensis and the application of the antimicrobial peptide in antibacterial drugs. The technical proposal is that two antimicrobial peptides are sequence-specific single-chain polypeptides separated from the amphibian skin secretions of the northeast Rana chensinensis; the two molecular weight values are 1459.9 and 2184.2 respectively; the two isoelectric points are 9.70 and 12.60, respectively; the amino acids sequences are SEQ ID NO.1 and SEQ ID NO.2 respectively. The preparation method is as follows: the skin secretions of the northeast Rana chensinensis are collected; after the sedimentation is removed through centrifugation, the skin secretions are frozen and dried, and then separated and purified through G50 and G25 molecular sieve chromatography and reversed-phase high pressure liquid chromatography to get a single composition. The invention can be applied to the preparation of the drugs used in the treatment of microbial infections.

The invention discloses a method for separating and extracting hyaluronic acid (HA) from rana chensinensis skins by using a membrane filtration process, and belongs to the field of methods for separating and extracting HA. According to the method disclosed by the invention, modern biological separation measures and methods such as membrane filtration are adopted, a ceramic membrane of which the membrane pore is 500nm is selected, the molecular weight cut off of the ceramic membrane is 1,000,000 Daltons, and thus protein can be effectively removed and HA can be intercepted. According to the method for extracting HA from the rana chensinensis skins, disclosed by the invention, 2.8g of high-quality HA can be extracted from per kilogram of the rana chensinensis skins, and by virtue of inspection, the glucuronic acid content of a product is 36%, and the protein content is 0.58%, so that the product can reach a cosmetic grade. According to the method, protein is removed by using the membrane filtration process, HA is desalted, graded and concentrated, high yield can be achieved, very little damage to the biological activity of polysaccharides can be caused, and the problem of reagent residues existing in a conventional organic solvent method also cannot be caused, so that the method is an economic, simple, convenient and effective extraction process and is suitable for industrial production of HA.

The invention belongs to the technical field of peptides and in particular relates to a rana chensinensis egg peptide as well as a preparation method and an application thereof. The preparation method comprises the following process steps: grinding and screening degreased rana chensinensis eggs; performing enzymolysis on an enzyme system combination of trypsin and papain; reacting to set time and ending the reaction; centrifuging twice, and mixing the supernatants; and performing freeze drying on the supernatant, and grinding, thereby obtaining the rana chensinensis egg peptide. The rana chensinensis egg peptide serves as a biological active peptide, can be applied to multiple aspects in medicines and diets and has high antioxidant activity, and the method provided by the invention is simple and easy to operate, and the yield of the peptide is high.

The invention relates to a zymolysis method for extracting an oligopeptide mixture with high F value by Rana temporaria eggs. The method has the steps as follows: (1) after are compressing dried Rana temporaria eggs, and egg oil is filtered to obtain egg residue for standby; (2) the egg residue is placed in a reaction kettle and purified water is added, stirred and heated to 48 DEG C to 52 DEG C; and then after pH value is regulated to 8.0 to 8.5, peptide compound enzyme with the weight to be 1 percent to 3 percent of that of the egg residue is added for hydrolysis for 4h to 6h; the temperature is heated up to 80 DEG C to 85 DEG C, and insulated for 10min to 20min; and filtering is carried out to obtain filtrate which is frozen, dried and shattered to obtain the oligopeptide mixture with high F value. Or step (2) can be as follows: pH value is regulated to 1.5 to 2.5, pepsin with the weight to be 1 percent to 3 percent of the egg residue is added for hydrolysis for 4h to 6h; and after the pH value is regulated to 6 to 7, actin enzyme with the weight to be 0.5 percent to 1.5 percent of that of the egg residue is added for hydrolysis for 5h to 7h; and then the temperature is raised to 85 DEG C to 95 DEG C till finally the oligopeptide mixture with high F value is obtained. The method is characterized by reasonable technique, high yield and recyclable egg residue waste.

The invention relates to a breeding cage, in particular to a rana chensinensis breeding cage and aims to solve the problems that existing rana chensinensis is fed on soil or vegetation, excrement of rana chensinensis on the soil fails to be timely removed, a large number of bacteria are bred to cause cross infection and cause diseases to ran chensinensis, and rana chensinensis is caused to death in a large area. The rana chensinensis breeding cage comprises a cage rack, a pool body, a rana chensinensis cage mesh and a sewage discharge ditch mesh. The rana chensinensis cage mesh is laied on the periphery of the cage rack, cage legs are arranged below the rana chensinensis cage mesh, the pool body is arranged at the bottom of a cavity of the rana chensinensis mesh, the upper end of the pool body is provided with a pool surface and a feed table on the periphery from inside to outside and from low to high, the middle of the pool surface is provided with a sewage discharge ditch longitudinally, the sewage discharge ditch mesh is arranged above the sewage discharge ditch, the bottom of the sewage discharge ditch is provided with a sewage discharge outlet, frist slopes from inside to outside and from low to high are arranged on the pool surface, and a pool arranged between the top end of the pool surface and the lower end of the feed table is 0-50mm in height. The rana chensinensis breeding cage is used for breeding rana chensinensis.

The invention relates to a compounded traditional Chinese medicinal rana chensinensis extract preparation and an application thereof in preparation of a medicine and a cosmetic for treating and preventing acnes, and belongs to the technical field of traditional Chinese medicine preparations. A preparation method of the preparation comprises the following steps: removing impurities and dust from dried rana chensinensis and smashing, then adding a compound enzyme hydrolysate and centrifuging to obtain a supernate at an upper layer; grinding the rest of mixtures, then shearing and carrying out spray drying to form powder, adding the supper micropowder into an acetic acid and carrying out ultrasonic treatment, then filtering the micropowder with a gauze and centrifuging to obtain a supernate, and carrying out reduced pressure concentration on the supernate so as to obtain a mixture of polysaccharides, antibacterial peptides and calcium ions; mixing the mixtures so as to obtain a mixed rana chensinensis extracting liquid; mixing the mixed rana chensinensis extracting liquid with a compounded medicine extracting liquid so as to obtain the compounded traditional Chinese medicinal rana chensinensis extract preparation. The obtained preparation is a traditional Chinese medicinal preparation which has no side effect and can be used for supplying sufficient nutrition to cells and restoring injured cells, wherein the antibacterial peptides can be combined with the calcium ions so as to enhance a bactericidal effect. The obtained preparation is nonirritating to skin mucosa, rapid in bactericidal speed, rapid in curing of nidus positions, simple and convenient to use, colorless and odorless, and is suitable for people in different ages.

A chewing tablet of tree frog contains enzymolized tree frog liquid, milk powder and soybean powder proportionally. It is prepared from the tree frog in hibernation through washing, drying in the air, breaking, mixing with distilled water, enzymolyzing, deactivating enzyme, filtering, centrifugal liquid-solid separation, mixing the enzymolytic liquid with milk powder and soybean powder, drying and tabletting.

The invention discloses a method for wintering of rana chensinensis in northern Guangdong mountains. The method comprises the following steps of: establishing a raising-wintering integrated chamber which can be used as an indoor rana chensinensis raising lot and also can be used as a rana chensinensis wintering chamber, and performing initial rana chensinensis wintering management, rana chensinensis wintering deep-sleep period management and rana chensinensis wintering later period management in the raising-wintering integrated chamber. The method for wintering of the rana chensinensis in the northern Guangdong mountains is formulated according to the combination of the physiological habits of the rana chensinensis under northeast climatic environment and wintering environment and the natural environment of the northern Guangdong mountains and is an environment and wintering management measure suitable for safe wintering of the rana chensinensis in the northern Guangdong mountains. The method for wintering of the rana chensinensis in the northern Guangdong mountains is generally from the last ten days of December when the rana chensinensis does not take food to the middle ten days of March of the next year when the rana chensinensis can take food. By adopting the method for wintering of the rana chensinensis in the northern Guangdong mountains, the rana chensinensis can winter in the northern Guangdong mountains safely. By practical operation of the inventor in the northern Guangdong mountains, the survival rate of the rana chensinensis in the northern Guangdong mountains reaches over 80 percent.

An oral liquid of tree frog skin contains the enzymolized liquid of tree frog skin, honey, liquid extract of acanthopanax bark and distilled water proportionally. It is prepared from the skin of fresh tree frog through washing, drying in the air, breaking, mixing with distilled water, enzymolyzing, deactivating enzyme, filtering, centrifugal liquid-solid separation, and mixing the enzymolytic liquid with honey, liquid extract of acanthopanax bark and distilled water.

The invention discloses health passion fruit and honeysuckle tea particles which are prepared from the following raw materials in parts by weight: 100-120 parts of green tea powder, 20-30 parts of passion fruits, 60-80 parts of honeysuckle flowers, 20-30 parts of barley seedlings, 10-12 parts of pear juice, 10-11 parts of galangal, 5-6 parts of fried jujube kernels, 6-8 parts of dried pawpaw, 7-8 parts of rana chensinensis, 7-9 parts of peanut germs, 6-8 parts of cassava starch, 1-2 parts of radix scrophulariae, 3-4 parts of burdocks, 2-5 parts of rhizoma polygonati odorati, 3-4 parts of gynura procumbens, 1-2 parts of gastrodia elata, 8-10 parts of maltodextrin, 1.8-2 parts of vitamin C, a proper amount of ethanol, a proper amount of water and 8-9 parts of nutritional addition liquid. According to the health passion fruit and honeysuckle tea particles provided by the invention, the added vitamin C is used for enhancing the oxidation resistance of the green tea powder, so that flavanol compounds in the green tea powder are hardly oxidized, and therefore, the original color of the green tea powder is retained; the added barley seedlings can be used for improving the color of the health tea particles, so that the mixed tea is light yellowish green and has attractive color and luster.

The invention relates to the technical field of Rana chensinensis breeding and especially relates to a breeding shed for Rana chensinensis and a breeding method thereof. A breeding shed for Rana chensinensis comprises a fence and a ceiling which are characterized in that the ceiling is installed at the upper end of the fence; the fence is a rectangle framework structure formed by multiple fixed piles and nylon window screen; the internal fence is provided with a living area for Rana chensinensis in the center, a trench is arranged at the peripheral of the Rana chensinensis living area, and a walkway is installed at the peripheral of the trench; the ceiling presents an umbrella-shaped structure, a part, from the center to the two-thirds of the periphery, of the ceiling is a weak light layer, and a part, from the periphery to the one-third of the center, of the ceiling is a dark layer. Compared with the prior art, the breeding shed for <{EN0}>Rana chensinensis and the breeding method thereof has simple and secure structure, is easy to construct on site, and has good cooling and ventilation effect; the pavilion-type fence shed satisfies the demand of free lighting, feeding on food platform, motion and comfortable inhabitation; temperature and humidity are adjustable; the cooling and moisturizing effect is obvious in hot summer days so that <{EN0}>Rana chensinensis can safely live through summer.

The invention aims to improve the discarded rana chensinensis skin into practical leather products, relates to the field of tanning processing and mainly relates to a processing technology for tanning and dyeing by taking the rana chensinensis skin as a raw material. The invention provides the technical scheme for solving the problems that the rana chensinensis skin is discarded as waste but the excellent cortex air-permeability, waterproof property, excellent flexibility, rich style and anti-bacteria effect of the rana chensinensis skin cannot be fully utilized.

The invention relates to puerperium care wine for women who have given birth to a baby, and specifically to rana chensinensis puerperium care wine and a preparation method thereof. The invention mainly aims to overcome the technical problem that pure puerperium care wine does not have a remarkable effect on lactation promotion and recovery of uterus after childbirth. A technical scheme provided in the invention is as follows: the rana chensinensis puerperium care wine comprises, by weight percent, 95 to 99% of puerperium care rice wine, 1 to 5% of rana chensinensis extract, 0 to 3% of matrimony vine extract, 0 to 3% of safflower extract and 0 to 3% of red date extract. The rana chensinensis puerperium care wine prepared in the invention is applicable to women who have just given birth to a baby, especially to women who have just given birth to a baby one month before.

The invention relates to a rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K and application thereof in preparation of an escherichia coli resistant drug, a multi-drug resistant escherichia coli H1 resistant drug, a Klebsiella pneumoniae resistant drug, a psychrobacter faecalis resistant drug, an acinetobacter baumannii resistant drug, a shigella dysenteriae resistant drug, a staphylococcus aureus resistant drug, a bacillus subtilis resistant drug and a micrococcus luteus resistant drug. The rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K comprises 26 amino acid residues. The molecular weight of the rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K is 2613.21 Da, the theoretical isoelectric point of the rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K is 10.60, and the sequence is GLLSKFKGVLKTAGKNVAKNVAGSLL. The rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K disclosed by the invention has the advantages of simple structure, low cytotoxicity, convenience for artificial synthesis and the like.

The invention provides a rana chensinensis extracted mixed liquid and an application thereof in preparing medicines for treating and preventing dermatophytosis, and belongs to the technical field of medical bio-pharmacy. The mixed liquid is prepared by adopting the following steps: performing impurity-removal and dust-collection on dried rana chensinensis, crushing, adding complex enzyme hydrolysate, and centrifuging to obtain upper supernate; grinding remaining mixture, shearing, and drying by means of spray to obtain powder; adding the superfine powder into acetic acid, performing ultrasonic treatment, and filtering by virtue of a gauze element, centrifuging to obtain supernate, and concentrating at reduced pressure so as to obtain a mixture of polysaccharide, antimicrobial peptide and calcium ions; and mixing all the materials to obtain the rana chensinensis extracted mixed liquid. The mixed liquid is a pure biological preparation, and does not have side effect; micromolecular antimicrobial peptide with a remarkable antibacterial effect is combined with the calcium ions to reinforce destruction of the micromolecular antimicrobial peptide to fungal cell walls, and can enter fungal cells to achieve the antimicrobial effect; the oligopeptide collagen can enter normal cells in the action of polysaccharide to give sufficient nutrition to the cells, reinforce the defending function of the cells, helping the normal cells defend external damage factors and repairing injured cells.

The invention relates to an artificial propagation technology of rana chensinensis david. The artificial propagation technology comprises the following steps of selecting 3-4a wood frogs as frog parents, collecting the frog parents into an egg laying pond of an artificial propagation farm to lay eggs, and performing massive propagation by a natural incubation method; according to the actual conditions, determining the culture density of tadpoles in the propagation pond, feeding plant and animal feeds at fixed time and fixed amount, completely transforming in a transformation pool into young frogs, and then performing forest propagation. By adopting the method, the artificial propagation technology has the advantages that a large amount of young frogs with high resistivity and immunity can be propagated, the yield and benefits of wood frogs are greatly improved, and the development of rana chensinensis david propagation industry is promoted.

The invention discloses a Beta-CD inclusion suppository containing rana chensinensis antibacterial peptide for treating gynecological diseases, and also discloses a preparation method thereof. The suppository consists of liposoluble stroma, the antibacterial peptide, Beta-CD, azone and a moderate amount of aqueous mint solution and hardening agent. The suppository is applicable to the prevention or treatment of various directly or indirectly infected gynecological diseases and bacterial and mycotic diseases. An experiment shows that the bactericidal rate can reach more than 99 percent after the suppository acts on staphylococcus aureus, colibacillus and candida albicans for 15min; and an animal experiment shows that the antibacterial peptide suppository cannot cause any irritation on the skin, side effects and drug resistance.

The invention discloses a method for researching the anti-fatigue pharmacological action of rana chensinensis oil freeze-dried powder. The method comprises the following steps: testing body weight andexhaustion time, researching the influence of the rana chensinensis oil freeze-dried powder on rat serum testosterone and corticosterone, researching the influence of the rana chensinensis oil freeze-dried powder on rat urea nitrogen, liver and myoglycogen reserves, and carrying out overall evaluation according to the researches. According to the research method, the rana chensinensis oil freeze-dried powder can be researched from multiple aspects of a fatigue causing mechanism, a systematic evaluation method is formed, and the research method has great significance in development of a rana chensinensis oil freeze-drying process and popularization of freeze-dried products.

The invention discloses a rana chensinensis functional gene Rd-RNase3 sequence which is characterized by being a nucleotide sequence shown in a sequence table SEQ ID No.1. In the invention, a tumor cell agglutination mediated by the rana chensinensis ribonuclease is not relevant to the selective cytotoxicity of tumor cells and the existence of the wild-type p53 tumor restraint substance, cannot produce gene mutation, and has better stability compared with the prior art. Long-term tumor restraint experiment proves that the Rd-RNase3 gene recombination fusion protein of the invention has strong cytotoxicity on the tumor cells under extremely low concentration, but has no cytotoxicity on normal cells, and thereby having the great advantage on preparing antitumor drugs compared with the existing gene drugs.

The invention discloses a rana chensinensis tremella dipped sea cucumber food formula, which is characterized by involving the following raw materials by weight part: 3-5 of salted sea cucumber, 0.3-0.5 of dry rana chensinensis, 0.5-0.7 of dry tremella, 1-3 of dried lotus seed, 3-5 of fresh ginkgo, 2-4 of dried red dates, 7-9 of xylitol, and 70-90 of purified water. The rana chensinensis tremella dipped sea cucumber provided by the invention solves the problems of difficult making and single taste of sea cucumber food.

The invention discloses a rana chensinensis food for tonifying kidney, reinforcing premodial qi, resisting cancer and beautifying faces and a preparation method thereof. The rana chensinensis food is prepared from rana chensinensis, glossy ganoderma, ginseng, hairy antler, prepared rehmannia root, Chinese wolfberry, common macrocarpium fruit, cistanche, cinnamomvine, semen sesami nigrum and mushroom mycelia. The preparation method comprises the following steps of: drying the rana chensinensis in a weight ratio, and grinding into rana chensinensis powder for later use; separating the glossy ganoderma from water, steaming over slow fire for 40 minutes, taking the steamed glossy ganoderma out, airing or drying, and grinding into powder for later use; putting the dried semen sesami nigrum and the Chinese wolfberry into the mixture of the hairy antler, the ginseng, the mushroom mycelia, the cinnamomvine, the common macrocarpium fruit, the prepared rehmannia root and the cistanche, and grinding into powder; and blending the three groups of powder, and stirring uniformly. The rana chensinensis food has the effects of tonifying kidney, reinforcing premodial qi, resisting cancer and beautifying faces, and is comprehensive in nutrition, easy to absorb and obvious in effect; and compared with the conventional single rana chensinensis which is eaten directly, the rana chensinensis food has the advantages that nutritional ingredients are comprehensive and easy to absorb, and a health-care effect is obvious.

The invention discloses a health food containing a rana chensinensis egg extract. The health food containing the rana chensinensis egg extract is micro-capsules which are prepared by taking whey protein and beta-cyclodextrin as wall materials, as well as the rana chensinensis egg extract, ginsenoside, soybean isoflavone and collagen as core materials wherein, the mass ratio of the core materials to the wall materials is (1-2) to 1, the mass ratio of the whey protein to the beta-cyclodextrin is (1-2) to 1, and the mass ratio of the rana chensinensis egg extract to the ginsenoside to the soybeanisoflavone to the collagen is (1-3) to (1-2) to (1-2) to (1-2). The health food containing the rana chensinensis egg extract disclosed by the invention is rich in a plurality of ingredients, high innutritional values, convenient to eat, easy to absorb and purely natural, and has the functions of reducing blood lipid, reducing cholesterol, lowering blood pressure, preventing and treating cardiovascular and cerebrovascular diseases, preventing and resisting cancer, improving Immune functions, beautifying, removing freckles, delaying and resisting senility, repairing ovarian and so on.

The invention provides a method for extracting hyaluronic acid from rana chensinensis skin, which comprises the following steps of: (1) obtaining rana chensinensis skin powder; (2) dissolving the ranachensinensis skin powder by using distilled water; (3) carrying out super-high pressure treatment on the rana chensinensis skin powder dissolving liquid; (4) carrying out enzymolysis treatment on thesuper-high pressure leaching liquid by using trypsin and alkaline protease; (5) adding ethanol to the enzymolysis liquid to generate precipitate, carrying out centrifugation, collecting the precipitate, and carrying out drying treatment; (6) dissolving the dried precipitate by using sodium chloride; (7) adding hydrogen peroxide to the precipitate dissolving liquid, and carrying out decoloration;(8) adjusting the pH value to 4.5-5.0, adding the mixed solution of chloroform and n-butanol, carrying out centrifugation to separate protein, and collecting the water phase; (9) dialyzing the water phase, and collecting the dialysate; and (10) carrying out alcohol precipitation and drying treatment on the dialysate to obtain the hyaluronic acid fine product. According to the present invention, the method has advantages of simple process and high hyaluronic acid extraction rate, and the extracted hyaluronic acid has high purity and good activity.