50 results about "Rana chensinensis" patented technology

A pichia pastoris genetic engineering hybrid antibacterial peptide CC29 and a preparing method thereof are disclosed. Musca domestica cecropin obtained by cloning in a laboratory and rana chensinensis Chensirin are adopted as parent peptides and are subjected to hybrid designing to obtain the novel hybrid peptide with low hemolysis and high antibacterial activity and a fermentation strain capable of efficiently expressing the novel hybrid peptide. Compared with natural peptides, the peptide has huge advantages. A foundation of large-scale production of the antibacterial peptide is laid.

The invention provides minimally invasive repair gel with rana chensinensis skin polypeptide. The minimally invasive repair gel is composed of, by mass, 100 parts of rana chensinensis skin polypeptide, 65 parts of acrylic acid(ester)/C10-30 alkanol acrylic ester cross-linked polymer, 200 parts of trehalose, 5 parts of ethylene diamine tetraacetic acid, 300 parts of glycerinum, 10 parts of carboxymethyl beta-dextran sodium, 300 parts of butanediol, 70 parts of 1,2-hexanediol, 25 parts of phenoxyethanol, 15 parts of PEG-40 hydrogenated castor oil, 100 parts of hydrolyzed royal jelly protein, 26 parts of palmitoyl tripeptide-5 and 4 parts of sodium hyaluronate. Rana chensinensis skin polypeptide serves as a main component, thickening agents, wetting agents, skin conditioning agents, chelating agents, solubilizing agents and the like serve as auxiliary components, and then the gel with a bacterium restraining function and a wound repairing function is prepared. Rana chensinensis skin polypeptide is prepared through a neutral protease K enzymolysis method, and therefore the polypeptide molecular weight can be smaller than 2000 Da, and the polypeptide can be more easily absorbed by human skin; meanwhile, the pH value of the prepared gel finished product ranges from 5.5 to 7.5, and the stimulus to skin is avoided.

The invention relates to a preparation method of rana chensinensis frogspawn calcium amino acid chelated food, belonging to the agricultural product processing technique. The preparation method comprises the following steps: taking frogspawns as raw materials, drying, powdering, extracting and degreasing to obtain purified degreased frogspawn powder; under mild conditions, preparing frogspawn amino acid compound hydrolyzed solution by enzymolysis of various proteolytic enzymes; and then taking calcium chloride as a calcium source to prepare rana chensinensis frogspawn compound calcium amino acid chelated food by the chelation. In the method, waste frogspawns with abundant nutrition are prepared into healthcare food so as to spread the application range of the frogspawn. The method has thecharacteristics of reasonable and simple process and high utilization ratio of the frogspawns.

A Ca-enriched tablet of tree frog bone contains tree frog bone powder, granular white sugar, glucose and milk powder proportionally. It is prepared from tree frog bone through crushing, mixing it with distilled water, enzymolyzing, low-temp vacuum drying, pulverizing, mixing it with granular white sugar, glucose and milk powder, and tabletting.

The invention relates to an antimicrobial peptide separated from the skin of the northeast Rana chensinensis and the application of the antimicrobial peptide in antibacterial drugs. The technical proposal is that two antimicrobial peptides are sequence-specific single-chain polypeptides separated from the amphibian skin secretions of the northeast Rana chensinensis; the two molecular weight values are 1459.9 and 2184.2 respectively; the two isoelectric points are 9.70 and 12.60, respectively; the amino acids sequences are SEQ ID NO.1 and SEQ ID NO.2 respectively. The preparation method is as follows: the skin secretions of the northeast Rana chensinensis are collected; after the sedimentation is removed through centrifugation, the skin secretions are frozen and dried, and then separated and purified through G50 and G25 molecular sieve chromatography and reversed-phase high pressure liquid chromatography to get a single composition. The invention can be applied to the preparation of the drugs used in the treatment of microbial infections.

The invention relates to a zymolysis method for extracting an oligopeptide mixture with high F value by Rana temporaria eggs. The method has the steps as follows: (1) after are compressing dried Rana temporaria eggs, and egg oil is filtered to obtain egg residue for standby; (2) the egg residue is placed in a reaction kettle and purified water is added, stirred and heated to 48 DEG C to 52 DEG C; and then after pH value is regulated to 8.0 to 8.5, peptide compound enzyme with the weight to be 1 percent to 3 percent of that of the egg residue is added for hydrolysis for 4h to 6h; the temperature is heated up to 80 DEG C to 85 DEG C, and insulated for 10min to 20min; and filtering is carried out to obtain filtrate which is frozen, dried and shattered to obtain the oligopeptide mixture with high F value. Or step (2) can be as follows: pH value is regulated to 1.5 to 2.5, pepsin with the weight to be 1 percent to 3 percent of the egg residue is added for hydrolysis for 4h to 6h; and after the pH value is regulated to 6 to 7, actin enzyme with the weight to be 0.5 percent to 1.5 percent of that of the egg residue is added for hydrolysis for 5h to 7h; and then the temperature is raised to 85 DEG C to 95 DEG C till finally the oligopeptide mixture with high F value is obtained. The method is characterized by reasonable technique, high yield and recyclable egg residue waste.

The invention relates to a compounded traditional Chinese medicinal rana chensinensis extract preparation and an application thereof in preparation of a medicine and a cosmetic for treating and preventing acnes, and belongs to the technical field of traditional Chinese medicine preparations. A preparation method of the preparation comprises the following steps: removing impurities and dust from dried rana chensinensis and smashing, then adding a compound enzyme hydrolysate and centrifuging to obtain a supernate at an upper layer; grinding the rest of mixtures, then shearing and carrying out spray drying to form powder, adding the supper micropowder into an acetic acid and carrying out ultrasonic treatment, then filtering the micropowder with a gauze and centrifuging to obtain a supernate, and carrying out reduced pressure concentration on the supernate so as to obtain a mixture of polysaccharides, antibacterial peptides and calcium ions; mixing the mixtures so as to obtain a mixed rana chensinensis extracting liquid; mixing the mixed rana chensinensis extracting liquid with a compounded medicine extracting liquid so as to obtain the compounded traditional Chinese medicinal rana chensinensis extract preparation. The obtained preparation is a traditional Chinese medicinal preparation which has no side effect and can be used for supplying sufficient nutrition to cells and restoring injured cells, wherein the antibacterial peptides can be combined with the calcium ions so as to enhance a bactericidal effect. The obtained preparation is nonirritating to skin mucosa, rapid in bactericidal speed, rapid in curing of nidus positions, simple and convenient to use, colorless and odorless, and is suitable for people in different ages.

The invention discloses a method for wintering of rana chensinensis in northern Guangdong mountains. The method comprises the following steps of: establishing a raising-wintering integrated chamber which can be used as an indoor rana chensinensis raising lot and also can be used as a rana chensinensis wintering chamber, and performing initial rana chensinensis wintering management, rana chensinensis wintering deep-sleep period management and rana chensinensis wintering later period management in the raising-wintering integrated chamber. The method for wintering of the rana chensinensis in the northern Guangdong mountains is formulated according to the combination of the physiological habits of the rana chensinensis under northeast climatic environment and wintering environment and the natural environment of the northern Guangdong mountains and is an environment and wintering management measure suitable for safe wintering of the rana chensinensis in the northern Guangdong mountains. The method for wintering of the rana chensinensis in the northern Guangdong mountains is generally from the last ten days of December when the rana chensinensis does not take food to the middle ten days of March of the next year when the rana chensinensis can take food. By adopting the method for wintering of the rana chensinensis in the northern Guangdong mountains, the rana chensinensis can winter in the northern Guangdong mountains safely. By practical operation of the inventor in the northern Guangdong mountains, the survival rate of the rana chensinensis in the northern Guangdong mountains reaches over 80 percent.

An oral liquid of tree frog skin contains the enzymolized liquid of tree frog skin, honey, liquid extract of acanthopanax bark and distilled water proportionally. It is prepared from the skin of fresh tree frog through washing, drying in the air, breaking, mixing with distilled water, enzymolyzing, deactivating enzyme, filtering, centrifugal liquid-solid separation, and mixing the enzymolytic liquid with honey, liquid extract of acanthopanax bark and distilled water.

The invention discloses health passion fruit and honeysuckle tea particles which are prepared from the following raw materials in parts by weight: 100-120 parts of green tea powder, 20-30 parts of passion fruits, 60-80 parts of honeysuckle flowers, 20-30 parts of barley seedlings, 10-12 parts of pear juice, 10-11 parts of galangal, 5-6 parts of fried jujube kernels, 6-8 parts of dried pawpaw, 7-8 parts of rana chensinensis, 7-9 parts of peanut germs, 6-8 parts of cassava starch, 1-2 parts of radix scrophulariae, 3-4 parts of burdocks, 2-5 parts of rhizoma polygonati odorati, 3-4 parts of gynura procumbens, 1-2 parts of gastrodia elata, 8-10 parts of maltodextrin, 1.8-2 parts of vitamin C, a proper amount of ethanol, a proper amount of water and 8-9 parts of nutritional addition liquid. According to the health passion fruit and honeysuckle tea particles provided by the invention, the added vitamin C is used for enhancing the oxidation resistance of the green tea powder, so that flavanol compounds in the green tea powder are hardly oxidized, and therefore, the original color of the green tea powder is retained; the added barley seedlings can be used for improving the color of the health tea particles, so that the mixed tea is light yellowish green and has attractive color and luster.

The invention relates to the technical field of Rana chensinensis breeding and especially relates to a breeding shed for Rana chensinensis and a breeding method thereof. A breeding shed for Rana chensinensis comprises a fence and a ceiling which are characterized in that the ceiling is installed at the upper end of the fence; the fence is a rectangle framework structure formed by multiple fixed piles and nylon window screen; the internal fence is provided with a living area for Rana chensinensis in the center, a trench is arranged at the peripheral of the Rana chensinensis living area, and a walkway is installed at the peripheral of the trench; the ceiling presents an umbrella-shaped structure, a part, from the center to the two-thirds of the periphery, of the ceiling is a weak light layer, and a part, from the periphery to the one-third of the center, of the ceiling is a dark layer. Compared with the prior art, the breeding shed for <{EN0}>Rana chensinensis and the breeding method thereof has simple and secure structure, is easy to construct on site, and has good cooling and ventilation effect; the pavilion-type fence shed satisfies the demand of free lighting, feeding on food platform, motion and comfortable inhabitation; temperature and humidity are adjustable; the cooling and moisturizing effect is obvious in hot summer days so that <{EN0}>Rana chensinensis can safely live through summer.

The invention relates to a rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K and application thereof in preparation of an escherichia coli resistant drug, a multi-drug resistant escherichia coli H1 resistant drug, a Klebsiella pneumoniae resistant drug, a psychrobacter faecalis resistant drug, an acinetobacter baumannii resistant drug, a shigella dysenteriae resistant drug, a staphylococcus aureus resistant drug, a bacillus subtilis resistant drug and a micrococcus luteus resistant drug. The rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K comprises 26 amino acid residues. The molecular weight of the rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K is 2613.21 Da, the theoretical isoelectric point of the rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K is 10.60, and the sequence is GLLSKFKGVLKTAGKNVAKNVAGSLL. The rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K disclosed by the invention has the advantages of simple structure, low cytotoxicity, convenience for artificial synthesis and the like.

The invention provides a rana chensinensis extracted mixed liquid and an application thereof in preparing medicines for treating and preventing dermatophytosis, and belongs to the technical field of medical bio-pharmacy. The mixed liquid is prepared by adopting the following steps: performing impurity-removal and dust-collection on dried rana chensinensis, crushing, adding complex enzyme hydrolysate, and centrifuging to obtain upper supernate; grinding remaining mixture, shearing, and drying by means of spray to obtain powder; adding the superfine powder into acetic acid, performing ultrasonic treatment, and filtering by virtue of a gauze element, centrifuging to obtain supernate, and concentrating at reduced pressure so as to obtain a mixture of polysaccharide, antimicrobial peptide and calcium ions; and mixing all the materials to obtain the rana chensinensis extracted mixed liquid. The mixed liquid is a pure biological preparation, and does not have side effect; micromolecular antimicrobial peptide with a remarkable antibacterial effect is combined with the calcium ions to reinforce destruction of the micromolecular antimicrobial peptide to fungal cell walls, and can enter fungal cells to achieve the antimicrobial effect; the oligopeptide collagen can enter normal cells in the action of polysaccharide to give sufficient nutrition to the cells, reinforce the defending function of the cells, helping the normal cells defend external damage factors and repairing injured cells.

The invention relates to an artificial propagation technology of rana chensinensis david. The artificial propagation technology comprises the following steps of selecting 3-4a wood frogs as frog parents, collecting the frog parents into an egg laying pond of an artificial propagation farm to lay eggs, and performing massive propagation by a natural incubation method; according to the actual conditions, determining the culture density of tadpoles in the propagation pond, feeding plant and animal feeds at fixed time and fixed amount, completely transforming in a transformation pool into young frogs, and then performing forest propagation. By adopting the method, the artificial propagation technology has the advantages that a large amount of young frogs with high resistivity and immunity can be propagated, the yield and benefits of wood frogs are greatly improved, and the development of rana chensinensis david propagation industry is promoted.

The invention discloses a Beta-CD inclusion suppository containing rana chensinensis antibacterial peptide for treating gynecological diseases, and also discloses a preparation method thereof. The suppository consists of liposoluble stroma, the antibacterial peptide, Beta-CD, azone and a moderate amount of aqueous mint solution and hardening agent. The suppository is applicable to the prevention or treatment of various directly or indirectly infected gynecological diseases and bacterial and mycotic diseases. An experiment shows that the bactericidal rate can reach more than 99 percent after the suppository acts on staphylococcus aureus, colibacillus and candida albicans for 15min; and an animal experiment shows that the antibacterial peptide suppository cannot cause any irritation on the skin, side effects and drug resistance.

The invention discloses a method for researching the anti-fatigue pharmacological action of rana chensinensis oil freeze-dried powder. The method comprises the following steps: testing body weight andexhaustion time, researching the influence of the rana chensinensis oil freeze-dried powder on rat serum testosterone and corticosterone, researching the influence of the rana chensinensis oil freeze-dried powder on rat urea nitrogen, liver and myoglycogen reserves, and carrying out overall evaluation according to the researches. According to the research method, the rana chensinensis oil freeze-dried powder can be researched from multiple aspects of a fatigue causing mechanism, a systematic evaluation method is formed, and the research method has great significance in development of a rana chensinensis oil freeze-drying process and popularization of freeze-dried products.

The invention discloses a health food containing a rana chensinensis egg extract. The health food containing the rana chensinensis egg extract is micro-capsules which are prepared by taking whey protein and beta-cyclodextrin as wall materials, as well as the rana chensinensis egg extract, ginsenoside, soybean isoflavone and collagen as core materials wherein, the mass ratio of the core materials to the wall materials is (1-2) to 1, the mass ratio of the whey protein to the beta-cyclodextrin is (1-2) to 1, and the mass ratio of the rana chensinensis egg extract to the ginsenoside to the soybeanisoflavone to the collagen is (1-3) to (1-2) to (1-2) to (1-2). The health food containing the rana chensinensis egg extract disclosed by the invention is rich in a plurality of ingredients, high innutritional values, convenient to eat, easy to absorb and purely natural, and has the functions of reducing blood lipid, reducing cholesterol, lowering blood pressure, preventing and treating cardiovascular and cerebrovascular diseases, preventing and resisting cancer, improving Immune functions, beautifying, removing freckles, delaying and resisting senility, repairing ovarian and so on.

The invention provides a method for extracting hyaluronic acid from rana chensinensis skin, which comprises the following steps of: (1) obtaining rana chensinensis skin powder; (2) dissolving the ranachensinensis skin powder by using distilled water; (3) carrying out super-high pressure treatment on the rana chensinensis skin powder dissolving liquid; (4) carrying out enzymolysis treatment on thesuper-high pressure leaching liquid by using trypsin and alkaline protease; (5) adding ethanol to the enzymolysis liquid to generate precipitate, carrying out centrifugation, collecting the precipitate, and carrying out drying treatment; (6) dissolving the dried precipitate by using sodium chloride; (7) adding hydrogen peroxide to the precipitate dissolving liquid, and carrying out decoloration;(8) adjusting the pH value to 4.5-5.0, adding the mixed solution of chloroform and n-butanol, carrying out centrifugation to separate protein, and collecting the water phase; (9) dialyzing the water phase, and collecting the dialysate; and (10) carrying out alcohol precipitation and drying treatment on the dialysate to obtain the hyaluronic acid fine product. According to the present invention, the method has advantages of simple process and high hyaluronic acid extraction rate, and the extracted hyaluronic acid has high purity and good activity.