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Rana chensinensis extracted mixed liquid and application thereof in preparing medicines for treating and preventing dermatophytosis

A mixed solution and mixture technology are applied in the field of extracting mixed solution from Rana frondosa to prepare medicines for the treatment and prevention of beriberi, and can solve the problem that the molecular weight range of antibacterial peptides or collagen is not fixed, polysaccharides and calcium ions are not extracted, and effective ingredients are not utilized, etc. problems, to achieve rapid disappearance of clinical symptoms, improved defense capabilities, and significant curative effects.

Inactive Publication Date: 2014-12-03
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] None of the extraction methods in the prior art extract the polysaccharides and calcium ions contained in Rana, and only simply extract Rana antibacterial peptides, or simply extract Rana collagen, the most important thing is the molecular weight range of the extracted antibacterial peptides or collagen Not fixed, the difference is large, no detailed description, no reasonable use of active ingredients

Method used

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  • Rana chensinensis extracted mixed liquid and application thereof in preparing medicines for treating and preventing dermatophytosis
  • Rana chensinensis extracted mixed liquid and application thereof in preparing medicines for treating and preventing dermatophytosis
  • Rana chensinensis extracted mixed liquid and application thereof in preparing medicines for treating and preventing dermatophytosis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 Preparation of Rana Rana Extract Mixed Solution

[0059] Take 20g of dried forest frog (Chinese forest frog Changbai Mountain subspecies, purchased from Jilin Pharmacy), and after removing impurities and dust, grind it through 20 mesh, 50 mesh, and 100 mesh respectively, so that the particle size of the dried wood frog pulverization is below 500 μm, according to Add the compound enzyme hydrolyzate in the proportion of 1g:6mL, the compound enzyme hydrolyzate contains papain 3wt%, trypsin 2wt%, flavor protease 1wt%, water 94wt%, the enzymolysis temperature is 52℃, and the enzymolysis time is 1.5h, The pH of the enzymolysis is 7.2, then 87°C, 10min to inactivate the enzyme, the mixture is centrifuged at a temperature of 25°C, the centrifugation time is 3min, and the centrifugation speed is 2500rpm, the upper supernatant (25% of the total volume) is taken, and the The short peptide collagen contained in dried wood frog;

[0060] The content of arginine, lysine...

Embodiment 2

[0066] The preparation of embodiment two wood frog extract mixed liquid ointment

[0067] Prescription composition (by weight): dried wood frog 20g, paraffin 8g, liquid paraffin 6g, white vaseline 12g, glyceryl monostearate 10g, spar 40 9g, emulsifier OP3g, ethyl p-hydroxybenzoate 1g, borneol 1g.

[0068] Preparation:

[0069] (1) Preparation of Rana extract mixture

[0070] Weigh 20g of Rana dry (Chinese Rana subspecies of Changbai Mountain, purchased from Jilin Pharmacy), prepare Rana extract mixture according to the method of Example 1, and obtain Rana extract 53mL

[0071] (2) Preparation of oil phase: get 8g paraffin, 10g glyceryl monostearate, 12g white petrolatum, 6g liquid paraffin, 9g Span 40, 3g emulsifier OP, 1g ethyl p-hydroxybenzoate and grind and mix evenly to obtain oil phase;

[0072] (3) Preparation of Rana Rana Extract Ointment: Heat the oil phase prepared in step (2) and Rana Rana Extract Mixture to 90°C respectively, add Rana Rana Extract Mixture to the...

Embodiment 3

[0073] The preparation of embodiment three wood frog extract mixed solution spray

[0074] Prescription composition (by weight): dried wood frog 20g, sodium chloride 19mg PEG 400 23mg, benzalkonium bromide 6mg.

[0075] Preparation:

[0076] Weigh 20 g of dry Rana (Chinese Rana Changbai Mountain subspecies, purchased from Jilin Pharmacy), prepare a Rana extract mixture according to the method of Example 1, obtain 53 mL of Rana extract, add the above-mentioned amount of PEG4 400 , sodium chloride, and benzalkonium bromide, stirred at 120rpm for 10min, added water to 1000mL, stirred at 150rpm for 10min, fully mixed the above substances, and sterilized at 121°C for 10min. Cool down to room temperature by itself, divide into aerosol bottles (20mL / bottle), and obtain.

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Abstract

The invention provides a rana chensinensis extracted mixed liquid and an application thereof in preparing medicines for treating and preventing dermatophytosis, and belongs to the technical field of medical bio-pharmacy. The mixed liquid is prepared by adopting the following steps: performing impurity-removal and dust-collection on dried rana chensinensis, crushing, adding complex enzyme hydrolysate, and centrifuging to obtain upper supernate; grinding remaining mixture, shearing, and drying by means of spray to obtain powder; adding the superfine powder into acetic acid, performing ultrasonic treatment, and filtering by virtue of a gauze element, centrifuging to obtain supernate, and concentrating at reduced pressure so as to obtain a mixture of polysaccharide, antimicrobial peptide and calcium ions; and mixing all the materials to obtain the rana chensinensis extracted mixed liquid. The mixed liquid is a pure biological preparation, and does not have side effect; micromolecular antimicrobial peptide with a remarkable antibacterial effect is combined with the calcium ions to reinforce destruction of the micromolecular antimicrobial peptide to fungal cell walls, and can enter fungal cells to achieve the antimicrobial effect; the oligopeptide collagen can enter normal cells in the action of polysaccharide to give sufficient nutrition to the cells, reinforce the defending function of the cells, helping the normal cells defend external damage factors and repairing injured cells.

Description

technical field [0001] The invention belongs to the technical field of medical biopharmaceuticals, and in particular relates to a wood frog extract mixture and its use in preparing medicines for treating and preventing beriberi. Background technique [0002] Athlete's foot is a very common skin disease. 70% to 80% of adults suffer from beriberi, but the severity is different. It is often aggravated in summer and alleviated in winter, and some people do not heal all the year round. The pathogenic bacteria are mostly Trichophyton and Epidermophyton species, the main species are Trichophyton rubrum, Trichophyton gypsum, Epidermophyton flocculus, Trichophyton rosea. The main reason why beriberi is not easy to cure is that these fungi have strong drug resistance. During the treatment process, the moist and anaerobic environment of the affected area is a strong breeding ground for the remaining fungi; at the same time, the patient's body contains fungi. When the administration ...

Claims

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Application Information

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IPC IPC(8): A61K35/56A61K9/06A61K9/12A61K9/08A61P17/00A61P31/10A47K10/16
Inventor 滕乐生李佺谢晶刘艳逯家辉孟庆繁张杰刘瑜刘博通滕利荣王迪周毓麟
Owner JILIN UNIV
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