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Rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K and application thereof

A technology of B2-N26-V5K and brevinin-2ce, which is applied in the field of peptides, can solve the problem that skin active peptide substances are not much studied, and achieve the effects of convenient artificial synthesis, good inhibitory effect and strong killing effect.

Inactive Publication Date: 2015-03-04
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The application of amphibian drugs has a long history in my country, but the research on its active ingredients and pharmacological properties mainly focuses on small organic molecules such as alkaloids, and there are not many studies on its skin active peptides

Method used

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  • Rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The Chinese Rana antimicrobial peptide Brevinin-2CE modified body, named B2-N26-V5K, contains 26 amino acid residues, molecular weight 2613.21Da, theoretical isoelectric point 10.60, sequence GLLSKFKGVLKTAGKNVAKNVAGSLL.

[0019] The synthesis method of the above-mentioned Rana antimicrobial peptide Brevinin-2CE modified body B2-N26-V5K is as follows:

[0020] (1) Designing the Sequence of the Chinese Rana Antimicrobial Peptide Brevinin-2CE Modified Body

[0021] The protein sequence number of Rana chinensis Brevinin-2CE is GenBank: ADD51562.1. By truncating the C-terminal 11 amino acids of Brevinin-2CE and replacing the fifth valine with lysine, an antimicrobial peptide Brevinin from Rana chinensis was designed The sequence of the -2CE modified body is GLLSKFKGVLKTAGKNVAKNVAGSLL, which is a linear polypeptide containing 26 amino acid residues.

[0022] (2) Synthesis of Chinese Rana antibacterial peptide Brevinin-2CE modified body B2-N26-V5K

[0023] The whole sequence...

Embodiment 2

[0028] The use of Chinese Rana antibacterial peptide Brevinin-2CE modified body B2-N26-V5K (hereinafter referred to as B2-N26-V5K) in the preparation of anti-Escherichia coli drugs is as follows:

[0029] B2-N26-V5K 2000 μg

[0030] Add water for injection to 1000mL

[0031] Prepare injections according to the conventional method of pharmaceutical injections, each milliliter contains 2 μg of active ingredient B2-N26-V5K, and the dose for adults is intramuscularly injected once a day, 1 bottle of 2 mL each time.

Embodiment 3

[0033] The use of B2-N26-V5K in the preparation of anti-multidrug-resistant Escherichia coli H1 medicines is as follows:

[0034] B2-N26-V5K 4000μg

[0035] Add water for injection to 1000mL

[0036] Prepare injections according to the conventional method of pharmaceutical injections, each milliliter contains 4 μg of active ingredient B2-N26-V5K, and the dose for adults is injected intramuscularly once a day, 1 bottle of 2 mL each time.

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Abstract

The invention relates to a rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K and application thereof in preparation of an escherichia coli resistant drug, a multi-drug resistant escherichia coli H1 resistant drug, a Klebsiella pneumoniae resistant drug, a psychrobacter faecalis resistant drug, an acinetobacter baumannii resistant drug, a shigella dysenteriae resistant drug, a staphylococcus aureus resistant drug, a bacillus subtilis resistant drug and a micrococcus luteus resistant drug. The rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K comprises 26 amino acid residues. The molecular weight of the rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K is 2613.21 Da, the theoretical isoelectric point of the rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K is 10.60, and the sequence is GLLSKFKGVLKTAGKNVAKNVAGSLL. The rana chensinensis antibacterial peptide Brevinin-2CE modifier B2-N26-V5K disclosed by the invention has the advantages of simple structure, low cytotoxicity, convenience for artificial synthesis and the like.

Description

technical field [0001] The invention belongs to the technical field of polypeptides, and in particular relates to the Chinese Rana antibacterial peptide Brevinin-2CE modified body B2-N26-V5K. Background technique [0002] Brevinin-2 antimicrobial peptides were first isolated from the skin secretions of Japanese pond frog R.brevipoda in 1992. In recent years, brevinin-2 family antimicrobial peptides have also been found in frog stomach tissues. Brevinin-2 has broad-spectrum antibacterial activity, not only has very strong bactericidal activity against Gram-positive bacteria, Gram-negative bacteria, and some fungi, but also has effects on many clinical drug-resistant bacteria. In addition, brevinin-2 also has the function of stimulating insulin release. The pharmaceutical development of brevinin-2 contains huge value in the preparation of clinical therapeutic drugs. [0003] With the large-scale and inappropriate use of traditional antibiotics such as penicillin, microorgani...

Claims

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Application Information

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IPC IPC(8): C07K14/46A61K38/17A61P31/04
CPCA61K38/00C07K14/463Y02A50/30
Inventor 孙燕王晓艳李治
Owner SHAANXI NORMAL UNIV
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