Preparation method of rana chensinensis frogspawn calcium amino acid chelated food
A production method and amino acid technology, applied in food preparation, food science, application and other directions, can solve the problems of reducing the utilization value of the frog eggs, narrowing the scope of application, adverse effects, etc., achieving high utilization rate of frog eggs and ensuring continuous utilization. , the effect of protecting the ecological environment
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[0012] The embodiments of the present invention are described in detail below. A production method of wood frog egg amino acid chelated calcium food, the method comprises the following steps:
[0013] (1) Drying: put the collected frog ovum into a ventilated drying box and dry to constant weight at 55°C;
[0014] (2) crushing: crushing to 30 mesh;
[0015] (3) Extraction: using CO 2 Supercritical degreasing to prepare defatted frog egg powder; supercritical carbon dioxide solvent extraction pressure is 32MPa, temperature is 35°C; carbon dioxide flow rate is 20Kg / hour, extraction time is 6 hours;
[0016] (4) Enzymolysis: use papain 15 with an activity of 600,000 U / g and neutral protease 15 with an activity of 120,000 U / g to enzymatically hydrolyze for 3 hours at a temperature of 52°C and a pH value of 6 to make it Decompose into polypeptides and amino acids, etc., heat to above 80°C for 10 minutes, inactivate protease, filter, and prepare enzymatic hydrolysis solution;
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