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Rana chensinensis egg peptide as well as preparation method and application thereof

A wood frog egg and reaction technology, applied in the fields of hydrolyzed protein components, food science, digestive system, etc., can solve the problem of insufficient development and utilization of defatted wood frog egg protein, and achieve simple and easy operation, high antioxidant activity, The effect of high peptide yield

Pending Publication Date: 2016-02-03
SHENYANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the defatted Rana egg protein has not been fully developed and utilized in the prior art, the present invention provides a Rana egg polypeptide and its preparation method and application. The prepared Rana egg polypeptide can be added to the In daily diet, promote human health

Method used

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  • Rana chensinensis egg peptide as well as preparation method and application thereof
  • Rana chensinensis egg peptide as well as preparation method and application thereof
  • Rana chensinensis egg peptide as well as preparation method and application thereof

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Effect test

Embodiment 1

[0027] The method provided by the invention is used to prepare the Rana egg polypeptide, and the materials and instruments used are all commercially available.

[0028] 1) crushing and screening, 13g of degreased and dried Rana ovum is crushed with a portable high-speed traditional Chinese medicine grinder, and passed through a 60-mesh sieve;

[0029] 2) Enzymolysis, dilute the Rana roe powder prepared in step 1) by 2.5 times with distilled water, heat to 25°C and maintain a constant temperature, adjust the pH value of the system to 6 with 1mol / LNaOH solution or 1mol / LHCl solution, according to the enzyme activity The addition amount is 10400u / g material, add trypsin, papain, trypsin:papain enzyme activity ratio is 1.13, substrate concentration is 10%, enzymolysis temperature is 45℃, pH7.70, reaction time is 3.5h;

[0030] 3) Inactivate the enzyme, after reacting for 3.5 hours, adjust the pH value to 7.0 with 1mol / L NaOH solution or 1mol / L HCl solution, stop the reaction in a ...

Embodiment 2

[0034] 1) crushing and screening, 13g of degreased and dried Rana ovum is crushed with a portable high-speed traditional Chinese medicine grinder, and passed through a 60-mesh sieve;

[0035] 2) Enzymolysis, dilute the Rana roe powder prepared in step 1) by 3 times with distilled water, heat to 30°C and maintain a constant temperature, adjust the pH value of the system to 6 with 1mol / LNaOH solution or 1mol / LHCl solution, according to the enzyme activity The addition amount is 10400u / g material, add trypsin, papain, trypsin:papain enzyme activity ratio is 1.13, substrate concentration is 10%, enzymolysis temperature is 48.8℃, pH7.70, reaction time is 4h;

[0036] 3) Inactivate the enzyme, after reacting for 4 hours, adjust the pH value to 7.0 with 1mol / L NaOH solution or 1mol / L HCl solution, terminate the reaction in a water bath, the temperature of the water bath is 90°C, and the time of the water bath is 10 minutes;

[0037] 4) Centrifuge twice, combine the supernatant, centr...

Embodiment 3

[0040] 1) crushing and screening, 13g of degreased and dried Rana ovum is crushed with a portable high-speed traditional Chinese medicine grinder, and passed through a 60-mesh sieve;

[0041] 2) Enzymolysis, dilute the Rana roe powder prepared in step 1) by 3.5 times with distilled water, heat to 35°C and keep a constant temperature, adjust the pH value of the system to 6 with 1mol / LNaOH solution or 1mol / LHCl solution, according to the enzyme activity The addition amount is 10400u / g material, add trypsin, papain, trypsin:papain enzyme activity ratio is 1.13, substrate concentration is 10%, enzymolysis temperature is 55℃, pH7.70, reaction time is 4.5h;

[0042] 3) Inactivate the enzyme, after reacting for 4.5 hours, adjust the pH value to 7.0 with 1mol / L NaOH solution or 1mol / L HCl solution, terminate the reaction in a water bath, the temperature of the water bath is 95°C, and the time of the water bath is 12 minutes;

[0043] 4) Centrifuge twice, combine the supernatant, centr...

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Abstract

The invention belongs to the technical field of peptides and in particular relates to a rana chensinensis egg peptide as well as a preparation method and an application thereof. The preparation method comprises the following process steps: grinding and screening degreased rana chensinensis eggs; performing enzymolysis on an enzyme system combination of trypsin and papain; reacting to set time and ending the reaction; centrifuging twice, and mixing the supernatants; and performing freeze drying on the supernatant, and grinding, thereby obtaining the rana chensinensis egg peptide. The rana chensinensis egg peptide serves as a biological active peptide, can be applied to multiple aspects in medicines and diets and has high antioxidant activity, and the method provided by the invention is simple and easy to operate, and the yield of the peptide is high.

Description

technical field [0001] The invention belongs to the technical field of polypeptide preparation, and in particular relates to a Rana egg polypeptide and its preparation method and application. Background technique [0002] Rana Rana is widely distributed in Northeast my country. It integrates medicine, food and tonic, has high economic value, and is increasingly valued by domestic and foreign production, processing and distribution enterprises. Rana eggs have high nutritional value and have a variety of physiological functions. They can be used in pharmaceuticals and health food. After degreasing (supercritical extraction) and drying, the protein content of Rana eggs can reach more than 80%, and their amino acids are complete. , contains 9 kinds of essential amino acids required by the human body, the ratio of essential amino acids to total amino acids (E / T) is as high as 50%, and is rich in trace elements. It is a natural high-quality health food raw material. [0003] Bioa...

Claims

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Application Information

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IPC IPC(8): C12P21/06A23L33/18A61K38/01A61P1/14
Inventor 马岩吴帅孟宪军
Owner SHENYANG NORMAL UNIV
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