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Beta-CD inclusion suppository containing rana chensinensis antibacterial peptide and preparation method thereof

An antimicrobial peptide and clathrate technology, applied in the direction of antifungal agents, medical preparations containing active ingredients, antibacterial drugs, etc., can solve the problems of secondary infection, discomfort in use, non-healing of infection, etc., and achieve no discomfort , convenient administration and increased stability

Active Publication Date: 2010-10-13
TONGHUA KANGYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention discloses a β-CD inclusion complex suppository containing Rana antibacterial peptide, which is a dosage form with antibacterial and anti-inflammatory effects, definite and lasting curative effect, safety and non-irritation, and overcomes the discomfort and easy-to-cause of the existing medicines for patients. Non-healing infection or secondary infection defect

Method used

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  • Beta-CD inclusion suppository containing rana chensinensis antibacterial peptide and preparation method thereof
  • Beta-CD inclusion suppository containing rana chensinensis antibacterial peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Preparation of Rana antimicrobial peptide:

[0045] Weigh 20g of dried frog skin, soak in 500ml 5% acetic acid buffer solution at room temperature for 3 hours; centrifuge the obtained extract at 5000r.p.m for 30min, discard the precipitate, and keep the supernatant; boil the supernatant for 5min, cool, and 5000r.p.m. Centrifuge at p.m for 30 min, take the supernatant and pass through a 30KDa membrane to obtain the Rana antimicrobial peptide filtrate.

[0046] (2) Preparation of Rana antimicrobial peptide β-CD inclusion complex:

[0047] Take 50g of β-CD and add it to 240ml of water and mix it, grind it into a paste in a water bath at 50°C, add 10g of Rana antibacterial peptide, grind it thoroughly, and dry it in a constant temperature desiccator to obtain the product.

[0048] (3) Preparation of oily matrix:

[0049] Take 100g of semi-synthetic fatty acid glycerides, place them in an evaporating dish, heat them on a water bath, and make them fully melt to obtain a...

Embodiment 2

[0054] (1) Preparation of Rana antimicrobial peptide:

[0055]Weigh 15g of dried frog skin, soak in 450ml 5% acetic acid buffer solution at room temperature for 3 hours; centrifuge the obtained extract at 5000r.p.m for 30min, discard the precipitate, and keep the supernatant; boil the supernatant for 5min, cool, and 5000r.p.m. Centrifuge at p.m for 30 min, take the supernatant and pass through a 30KDa membrane to obtain the Rana antimicrobial peptide filtrate.

[0056] (2) Preparation of Rana antimicrobial peptide β-CD inclusion complex:

[0057] Take 30g of β-CD and add it to 300ml of water and mix it, grind it into a paste in a water bath at 50°C, add 6g of Rana antibacterial peptide, grind it thoroughly, and dry it in a constant temperature dryer to obtain the product.

[0058] (3) Preparation of oily matrix:

[0059] Take 75g of cocoa butter, put it in an evaporating dish, heat it on a water bath, and make it fully melt to obtain a uniform matrix solution.

[0060] (4) ...

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PUM

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Abstract

The invention discloses a Beta-CD inclusion suppository containing rana chensinensis antibacterial peptide for treating gynecological diseases, and also discloses a preparation method thereof. The suppository consists of liposoluble stroma, the antibacterial peptide, Beta-CD, azone and a moderate amount of aqueous mint solution and hardening agent. The suppository is applicable to the prevention or treatment of various directly or indirectly infected gynecological diseases and bacterial and mycotic diseases. An experiment shows that the bactericidal rate can reach more than 99 percent after the suppository acts on staphylococcus aureus, colibacillus and candida albicans for 15min; and an animal experiment shows that the antibacterial peptide suppository cannot cause any irritation on the skin, side effects and drug resistance.

Description

Technical field: [0001] The invention relates to a medicine for treating gynecological diseases, specifically provides a β-CD inclusion complex suppository containing Rana antibacterial peptide, and also discloses its preparation method, which belongs to the technical field of traditional Chinese medicine pharmacy. Background technique: [0002] At present, fungal, trichomonas, bacterial non-specific vaginitis and cervicitis are the most common diseases in women. Although there are many medicines for external use for the treatment of the above-mentioned diseases, the main dosage forms are lotion, common suppository, tablet, capsule, ointment, etc., but these dosage forms all have problems and deficiencies in varying degrees. Chinese patent CN99113318 "A kind of traditional Chinese medicine pill for treating gynecological diseases" discloses a kind of traditional Chinese medicine pill, i.e. Fengwei pill, which has the following disadvantages: first, there are many medicine pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/02A61K38/02A61K47/40A61P15/00A61P31/04A61P31/10A61P31/12A61P15/02
Inventor 滕利荣安金双孟庆繁逯家辉李青山周杰田晓乐高朝辉王贞佐
Owner TONGHUA KANGYUAN BIOLOGICAL TECH
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