Minimally invasive repair gel with rana chensinensis skin polypeptide

The technology of Rana wood and gel is applied in the field of minimally invasive repairing gel containing Rana Rana skin polypeptide and its preparation field, and can solve the problems of no public preparation method, unfavorable human skin absorption, excessive molecular weight of Rana Rana skin antibacterial peptide and the like

Inactive Publication Date: 2015-11-04
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, the Rana skin antimicrobial peptides in the above two patents are not conducive to human skin absorption due to excessive molecular weight
[0007] In addition, in the Chinese patent with publication number CN101491245, a preparation method of a disinfection product containing Rana antibacterial peptide is disclosed, but the patent does not disclose its preparation method

Method used

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  • Minimally invasive repair gel with rana chensinensis skin polypeptide
  • Minimally invasive repair gel with rana chensinensis skin polypeptide
  • Minimally invasive repair gel with rana chensinensis skin polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066](1) Wash the freshly stripped Rana Rana residue and peel off the skin, take 1000g of Rana Rana skin, mince the Rana Rana skin with a colloid mill, add 5000ml of distilled water to make a homogenate, add 10g of enzyme activity to 100000U / g neutral proteinase K, after enzymatic hydrolysis at 55°C for 4 hours, first filter with a 100-1000 mesh filter cloth, then pass through an ultrafiltration membrane with a molecular weight cut-off of 100kDa and an ultrafiltration membrane with a molecular weight cut-off of 10kDa, and then pass through an ultrafiltration membrane with a molecular weight cut-off of 10kDa 300Da nanofiltration membrane filtration, using the absorbance value at 220nm wavelength to monitor the peptide concentration (12 ~ 20mg / ml) in the retentate, when the peptide concentration is 18.33mg / ml, get 1200ml retentate, add 48g Glycine was freeze-dried to obtain 70 g of Rana skin polypeptide freeze-dried powder. The polypeptide content in the Rana skin freeze-dried ...

Embodiment 2

[0071] (1) Wash the freshly stripped Rana Rana residue and peel off the skin, take 500g of Rana Rana skin, mince the Rana Rana skin with a colloid mill, add 3750ml of distilled water to make a homogenate, add 5g of enzyme activity to 100000U / g Neutral proteinase K, after enzymatic hydrolysis at 60°C for 3 hours, first filtered through a 100-1000 mesh filter cloth and then filtered through an ultrafiltration membrane with a molecular weight cut-off of 100kDa and an ultrafiltration membrane with a molecular weight cut-off of 10kDa, and then filtered through an ultrafiltration membrane with a molecular weight cut-off of 300Da Nanofiltration membrane filtration, using the absorbance value at 220nm wavelength to monitor the polypeptide concentration (12-20mg / ml) in the retentate, when the polypeptide concentration is 14.17mg / ml, get 1200ml retentate, add 48g glycine to the retentate , after freeze-drying, 65 g of Rana skin polypeptide freeze-dried powder was obtained. The polypepti...

Embodiment 3

[0076] (1) Wash the freshly stripped Rana Rana residue and peel off the skin, take 250g Rana Rana skin, grind the Rana Rana skin with a colloid mill, add 2500ml distilled water to make a homogenate, add 2.5g of enzyme activity to 100000U / g of neutral proteinase K, after enzymatic hydrolysis at 65°C for 2 hours, first filter through a 100-1000 mesh filter cloth, then pass through an ultrafiltration membrane with a molecular weight cut-off of 100kDa and an ultrafiltration membrane with a molecular weight cut-off of 10kDa, and then pass through a molecular weight cut-off For 300Da nanofiltration membrane filtration, use the absorbance value at 220nm wavelength to monitor the peptide concentration (12~20mg / ml) in the retentate liquid, when the peptide concentration is 12.00mg / ml, get 500ml retentate liquid, add to the retentate liquid 20g of glycine was freeze-dried to obtain 26g of Rana skin polypeptide freeze-dried powder. The polypeptide content in Rana skin freeze-dried powde...

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Abstract

The invention provides minimally invasive repair gel with rana chensinensis skin polypeptide. The minimally invasive repair gel is composed of, by mass, 100 parts of rana chensinensis skin polypeptide, 65 parts of acrylic acid(ester)/C10-30 alkanol acrylic ester cross-linked polymer, 200 parts of trehalose, 5 parts of ethylene diamine tetraacetic acid, 300 parts of glycerinum, 10 parts of carboxymethyl beta-dextran sodium, 300 parts of butanediol, 70 parts of 1,2-hexanediol, 25 parts of phenoxyethanol, 15 parts of PEG-40 hydrogenated castor oil, 100 parts of hydrolyzed royal jelly protein, 26 parts of palmitoyl tripeptide-5 and 4 parts of sodium hyaluronate. Rana chensinensis skin polypeptide serves as a main component, thickening agents, wetting agents, skin conditioning agents, chelating agents, solubilizing agents and the like serve as auxiliary components, and then the gel with a bacterium restraining function and a wound repairing function is prepared. Rana chensinensis skin polypeptide is prepared through a neutral protease K enzymolysis method, and therefore the polypeptide molecular weight can be smaller than 2000 Da, and the polypeptide can be more easily absorbed by human skin; meanwhile, the pH value of the prepared gel finished product ranges from 5.5 to 7.5, and the stimulus to skin is avoided.

Description

technical field [0001] The invention belongs to disinfection products, and in particular relates to a minimally invasive repair gel containing Rana skin polypeptide and a preparation method thereof. Background technique [0002] Rana skin polypeptide has various activities such as antibacterial, anti-inflammatory, anti-oxidation, anti-aging, etc., and is related to the proliferation of skin cells, which can provide nutrients to the skin and delay skin aging. It is particularly noteworthy that the Rana skin polypeptide has important practical value and development potential because of its promotion of skin wound repair function. [0003] Gel is a semi-solid state without fluidity between the liquid state and the solid state. Gel is a huge spatial network structure, and the gaps in the structure are filled with a large amount of liquid. These liquids are wrapped in the gaps of the network structure and fixed, which makes the system lose its fluidity. Therefore, it has the fol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/02A61K35/65A61K9/06A61P17/00A61P31/04C12P21/06C07K1/34
Inventor 郝林琳李思明张宝元邓旭明张梅丁克祥于浩刘松财
Owner JILIN UNIV
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