43results about How to "No hemolytic activity" patented technology

The present invention relates to rana grahami antibiotic peptide and its application, and belongs to the field of biomedicine. Rana grahami antibiotic peptide is one kind of cyclic polypeptide separated from the secretion of amphibian rana grahami, and has molecular weight of 1705.3 dalton, isoelectric point of 9.39, and primary structure of polypeptide sequence Leu Lys Gly Cys Trp The Lys Ser Ile Pro Pro Lys Pro Cys Phe(LKGCWTKSIPPKPCF) with cysteines in the 4-th and 14-th forming intramolecular disulfide bond. Its preparation process includes collecting secretion of ether stimulated rana grahami, centrifuging to eliminate precipitate, freeze drying, chromatographic separation in filtering gel column and separating purification in reverse high pressure liquid chromatogram. The rana grahami antibiotic peptide has powerful effect of inhibiting bacteria, fungi and viruses and no hemolytic activity, and may be used in preparing medicine for treating pathogenic microbe infection diseases.

New use of a cryptoid crab antimicrobial peptide Scyreprocin. The antibacterial protein is derived from a cryptoid crab, and is expressed and purified by using a genetic engineered technology. It is first disclosed that the antimicrobial peptide Scyreprocin not only has good antimicrobial activity, but also has a membrane-penetrating function, has an inhibitory and killing activity against various cancer cells, and at the same time has no cytotoxicity against human non-cancer cells. It has demonstrated that the antibacterial polypeptide has a high efficiency and specific anti-cancer activity, shows great application value, and has a good application prospect in research, development and application as an anti-tumor pharmaceutical ingredient. Provided is new use of the cryptoid crab antibacterial peptide Scyreprocin, which broadens the application range of Scyreprocin, and provides more possibilities for the application of Scyreprocin.

The invention relates to a group of antioxidative small peptides for inhibiting melanin production and a preparation method and application thereof. The small peptides comprise a short peptide OGOT, a short peptide Magin-FL and a short peptide Ansin-FL and belong to the technical field of biomedicine. The three short peptides have strong anti-oxidative activity, can inhibit the activity of tyrosinase, can significantly reduce the production of melanin and have the characteristics of low molecular weight, simple structure, no cytotoxicity, no hemolysis activity, high tissue permeability and simple preparation method. The small peptides can be used to prepare novel skin care raw materials with effects of resisting oxidization and melanin production and fading spots, can be used for preparation of anti-oxidant drugs and food additives and have a good application prospect.

The invention relates to an anti-oxidization small peptide capable of inhibiting melanogenesis as well as a preparation method and application thereof. The anti-oxidization small peptide comprises a short peptide Ansin-2 and a short peptide Magin-2, and belongs to the technical field of biomedicines. The two short peptides have strong oxidization resistance and can inhibit the activity of tyrosinase and obviously reduce melanogenesis; meanwhile, the small peptide further has the characteristics of small molecular weight, simple structure, no cytotoxicity, no hemolytic activity and high tissue penetration, and the preparation method is simple. The anti-oxidization small peptide can be used for preparing a novel beauty-maintaining and skin-care raw material capable of resisting oxidization, resisting melanogenesis and removing spots, also can be used for preparing an anti-oxidization medicine, a food additive and the like, and has an extremely good application prospect.

The invention discloses a strain of Bacillus coagulans antagonizing streptococcus and its application. The bacterial strain has been preserved in the General Microorganism Center of China Microorganism Culture Collection Management Committee, and the preservation number is CGMCC No.12196. The bacterial strain is made into bacteria powder, which can be added to animal feed, and can also be used as a microbial preparation for antagonizing streptococci. Feeding tilapia with the feed containing the bacterial strain can effectively prevent and treat streptococcal disease. Mix the bacteria with Bacillus subtilis and Lactobacillus johnsonii to make a compound microecological preparation, which can improve the growth performance of tilapia, increase the activity of liver superoxide dismutase, catalase and lysozyme in serum . In addition, the fermented liquid of the bacteria can also be used as a culture environment improver. Sprinkling the fermented liquid of the above bacteria into the tilapia culture pond can reduce the number of streptococci in the culture water, and can also remove the streptococci in the water under low oxygen conditions. nitrite.

The invention discloses bacillus pumilus, a probiotics preparation and a preparation method and application thereof. The Bacillus pumilus LV149 is preserved in China center for type culture collection (CCTCC) in Nov. 23th, 2011, and the preservation number is CCTCC NO: M 2011411. The bacillus pumilus LV149 has strong extracellular protease, lipase and amylase activities, has wide rejection capability to vibrio and has no hemolytic activity. The Bacillus pumilus LV149 serves as fermenting bacterial strains and is performed with solid fermentation, drying and smashing so as to prepare bacillus pumilus probiotics preparation which uses Bacillus pumilus LV149 as the active ingredients. The bacillus pumilus probiotics preparation can be added into prawn feeds for feeding prawns, so that growth of prawn intestinal pathogenic vibrio can be restrained, prawn vibriosis can be reduced, simultaneously prawn growth is promoted, fish bait coefficient is reduced, quality of commodity is improved, culture cycle is shortened, accordingly culture risk and culture cost are reduced, biological safety is high and the bacillus pumilus, the probiotics preparation and the preparation method and application thereof have wide application prospect in aquaculture.

Disclosed is an alkaline antibacterial peptide consisting of leucine and lysine. The alkaline antibacterial peptide comprises 12-24 amino acids, wherein the proportion of lysine is more than 33.3%. Also disclosed is a targeted antibacterial peptide formed by the connection of the alkaline antibacterial peptide and the complementarity determining region of an antibody through leucine. The alkaline antibacterial peptide and the targeted antibacterial peptide can be used as an antibacterial and for cancer resistance.

The invention relates to the technical field of biomedical materials, in particular to a controllably degradable bone implantation composite material and a preparation method thereof. The bone implantation composite material comprises a nano bioglass material as a main structure, an amino acid polymer, and a modified calcium carbonate whisker / natural latex compound as a synergistic component, wherein graphene is added into the modified calcium carbonate whisker / natural latex compound serving as the synergistic component. According to the preparation method, the raw materials of the bone implantation composite material have good biological activity and biocompatibility, interaction between tissues and cells and the material is facilitated, the composite material has enough mechanical strength, meanwhile, the composite material can be rapidly degraded in the later period, the degradation rate of the bone implantation composite material can be adjusted by changing components, and the controllable degradation of the bone implantation composite material is realized.

The invention provides an antifungal antibacterial peptide GL4W and its preparation method and application. The sequence of the antibacterial peptide GL4W is shown in SEQ ID No.1. Taking the antimicrobial peptide L4 (RLRLLRLR) as the active center, a cap structure was formed with glycine at its N-terminus, and tryptophan was added at its C-terminus to form a tail anchor structure to design an antimicrobial peptide, which was named GL4W. Application of the antimicrobial peptide GL4W in the preparation of drugs for treating infectious diseases caused by fungi. The invention changes the antibacterial spectrum of antibacterial peptide L4, becomes a kind of antifungal antibacterial peptide, and improves the selectivity of antibacterial peptide to bacterial cells, which has the development potential of antifungal infection drug, and the therapeutic index of antibacterial peptide GL4W is as high as 39.61.

The invention provides a compound capable of increasing the skin anti-wrinkle function, and a use thereof in preparing a cosmetic. The cosmetic contains the modified compound, a modified group is a cell activity promoting peptide, the polypeptide can relatively well promote cell activity, and has collagen secretion promotion activity and anti-aging activity, after the polypeptide is coupled to thecompound, properties of the compound can be significantly synergistically promoted, and the anti-wrinkle effect of the skin is promoted. The compound after coupling itself has the characteristics oflow cytotoxicity, no hemolytic activity, simple structure, easy preparation and the like, so that the application of the compound in the field of anti-aging cosmetics has good market prospects.