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A compound capable of increasing skin anti-wrinkle function and its use in preparing cosmetics

A technology of anti-wrinkle cosmetics and compounds, applied in the field of biomedicine, can solve the problems that the improvement effect needs to be further improved, and achieve good market prospects, low cytotoxicity, and easy preparation

Active Publication Date: 2020-07-17
广州市新纪元化妆品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] CN201480017060.2 discloses a series of compounds that can improve the skin, but its improving effect needs to be further improved
[0008] However, there is still a lack of a highly effective and safe compound that increases the anti-wrinkle function of the skin in the market

Method used

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  • A compound capable of increasing skin anti-wrinkle function and its use in preparing cosmetics
  • A compound capable of increasing skin anti-wrinkle function and its use in preparing cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of coupling compound

[0023] Preparation of polypeptide-compound conjugate The compound of formula (II) of the present invention was synthesized according to the compound synthesis route in step A3 of Example A3 in CN201480017060.2. Then add 1mmol formula (II) compound, 1mmol O-benzotriazole-N,N,N',N'-tetramethylpirouentetrafluoroborate (TBTU), 5mlDMF, nitrogen protection in 50m reaction bottle, Add 0.5mmol) N, N-diisopropylhexylamine (DIEA) dropwise, stir at 25°C for 1.5h, add 0.1g of cell activity-promoting peptide (sequence VNHNYDFPWPDSEGKPPPHWLAKSPQK), stir at 36°C for 3.5h, and stop reaction. After confirming the correct molecular weight using MS-IT-TOF, the crude product was purified using HPLC to obtain a purified product with a purity of 99.96%. The structural formula of the conjugated product obtained through structural analysis is shown in formula (I): .

Embodiment 2

[0024] Example 2 Compound Toxicity Detection

[0025] (1) Cell culture

[0026] At 37°C, 5% CO 2 Human fibroblasts were prepared by culturing in DMEM medium containing 100 U / ml penicillin / streptomycin and 10% FBS in an incubator.

[0027] (2) Preparation of medium containing the compound of formula (I)

[0028] Mix DMEM powder (GIBCO, New York, USA) in the compound of Example 1, then add antibiotics (penicillin / streptomycin of 100U / ml) and 10% FBS to prepare the medium containing 5% formula (I) compound As well as the culture medium containing 50% of the compound of formula (I), the culture medium without the compound was used as a blank control, and the culture medium added with the compound of formula (II) was used as a positive control, and the concentrations were also 5% and 50% respectively.

[0029] (3) Cytotoxicity test of compounds

[0030] The human fibroblasts prepared in step (1) were added to a 24-well plate and cultured for 24 hours, and then the medium was re...

Embodiment 3

[0032] Example 3 Detection of COL1 protein expression by Western blotting electrophoresis

[0033]Human skin fibroblasts (HFF-1) were cultured in DMEM medium containing 15% fetal calf serum to the logarithmic phase of growth, digested with 0.25% trypsin, and seeded in 6-well plates at 105 / ml, each well 2ml. Cultivate the cells in a CO2 incubator at 37°C overnight to make the cells adhere to the wall. When the cell density grows to about 80%, the medium is aspirated, and the phosphate buffer solution (PBS: 0.8gNaCl; 0.2g KCl; 2.9g Na2HPO4.12H2O ; Dissolve 0.27g KH2PO4 in 800ml deionized water, stir to dissolve, dilute to 1L, adjust pH to 7.4 with concentrated HCl, autoclave) wash cells 2-3 times. Add the compound sample with a final concentration of 5% formula (I), add the compound sample with a final concentration of 5% formula (II) as a control, add 2% serum DMEM to the blank and incubate for 24 hours. Then discard the cell supernatant, centrifuge at 1000 g for 5 min to col...

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Abstract

The invention provides a compound capable of increasing the skin anti-wrinkle function, and a use thereof in preparing a cosmetic. The cosmetic contains the modified compound, a modified group is a cell activity promoting peptide, the polypeptide can relatively well promote cell activity, and has collagen secretion promotion activity and anti-aging activity, after the polypeptide is coupled to thecompound, properties of the compound can be significantly synergistically promoted, and the anti-wrinkle effect of the skin is promoted. The compound after coupling itself has the characteristics oflow cytotoxicity, no hemolytic activity, simple structure, easy preparation and the like, so that the application of the compound in the field of anti-aging cosmetics has good market prospects.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a compound capable of increasing skin anti-wrinkle function and its application in preparing cosmetics. Background technique [0002] According to the source of its composition, cosmetics can be divided into cosmetics of natural origin and cosmetics of compound origin. Most of the polymer compounds commonly used in cosmetics are water-soluble polymer compounds. Water-soluble polymer compounds, also known as water-soluble resins or water-soluble polymers, are hydrophilic polymer materials that can be dissolved or swelled in water to form solution or dispersion. The hydrophilicity of water-soluble polymers comes from the hydrophilic genes contained in their molecules. The most common hydrophilic groups are carboxyl, hydroxyl, amide, amine, ether, etc. These groups not only make the polymer hydrophilic, but also make it have many valuable properties, such as adhesion, film-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/00A61K8/64A61Q19/08
CPCA61K8/64A61Q19/08C07K14/00
Inventor 彭菲李静
Owner 广州市新纪元化妆品有限公司
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