Ethyl acetate extract of Chinese caterpillar fungus, preparation method and use thereof in antiviral medicine
A technology of ethyl acetate extraction and ethyl acetate, which is applied to antiviral agents, pharmaceutical formulations, plant raw materials, etc., can solve the problems of incomplete extraction of active ingredients and few reports on research on antiviral effects, and achieve no plasma coagulation activity. , good antiviral ability, improve the effect of drug efficacy
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Embodiment 1
[0031] Preparation of ethyl acetate extract of Cordyceps militaris:
[0032] (1) Grind the dried Cordyceps militaris mycelium to obtain 20 g of mycelium powder, add 200 ml of ethyl acetate, extract with a Soxhlet extractor at 77° C. for 8 hours under reflux, and filter to obtain filtrate 1.
[0033] (2) Extract 25.8 g of the filter residue with 258 ml of deionized water at 100°C for 3 hours, and filter to obtain filtrate 2.
[0034] (3) Filtrate 1 and filtrate 2 were combined, and concentrated under reduced pressure to constant weight to obtain the ethyl acetate extract of Cordyceps militaris.
Embodiment 2
[0036] Preparation of ethyl acetate extract of Cordyceps militaris:
[0037] (1) Grind the dried Cordyceps mycelium, take 35 g of mycelium powder, add 350 ml of ethyl acetate, extract with a Soxhlet extractor at 93° C. for 11 h under reflux, and filter to obtain filtrate 1.
[0038] (2) Extract 40.5 g of filter residue with 405 ml of deionized water at 100° C. for 3 h, and filter to obtain filtrate 2 .
[0039] (3) Filtrate 1 and filtrate 2 were combined, and concentrated under reduced pressure to constant weight to obtain the ethyl acetate extract of Cordyceps militaris.
Embodiment 3
[0041] Preparation of ethyl acetate extract of Cordyceps militaris:
[0042] (1) Crush the dried Cordyceps militaris mycelium to obtain 43 g of mycelium powder, add 430 ml of ethyl acetate, extract with a Soxhlet extractor at 86° C. for 9 hours, and filter to obtain filtrate 1.
[0043] (2) Extract 48.7 g of filter residue with 487 ml of deionized water at 100° C. for 4.5 h, and filter to obtain filtrate 2.
[0044] (3) Filtrate 1 and filtrate 2 were combined, and concentrated under reduced pressure to constant weight to obtain the ethyl acetate extract of Cordyceps militaris.
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