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Preparation method of ganoderma lucidum polysaccharide with high yield

A high-yield technology of ganoderma polysaccharides, applied in the field of preparation of ganoderma polysaccharides, can solve the problems of not considering the recovery of water-soluble ganoderma polysaccharides, limited extraction rate, waste of resources, etc., and achieve high yield, low extraction temperature and short time Effect

Active Publication Date: 2010-08-18
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the current research results, it can be seen that the extraction rate of water-soluble ganoderma lucidum polysaccharides is only about 3%, and the extracted ganoderma lucidum residue also contains some other polysaccharides, such as alkali-soluble proteoglycans and amino polysaccharides. If they are discarded directly , not only waste resources, but also pollute the environment
Simple extraction of alkali-soluble polysaccharides of Ganoderma lucidum, because the recovery of water-soluble polysaccharides of Ganoderma lucidum is not considered, the extraction rate is also very limited

Method used

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  • Preparation method of ganoderma lucidum polysaccharide with high yield
  • Preparation method of ganoderma lucidum polysaccharide with high yield
  • Preparation method of ganoderma lucidum polysaccharide with high yield

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Use red ganoderma lucidum fruiting bodies as raw materials to carry out ultrafine pulverization treatment to obtain red ganoderma powder with a particle size of about 150 meshes. Red ganoderma powder is refluxed twice with 90% ethanol at 80° C. for 3 hours each time. The ratio of solid to liquid is 1:15 (g / ml), then centrifuged to remove the supernatant, and the resulting precipitate is dried in an oven at 60°C for 5 hours to obtain Ganoderma lucidum from which alcohol-soluble components, some monosaccharides, oligosaccharides and lipid substances have been removed pink.

[0039] (2) Accurately weigh 5g of red Ganoderma lucidum powder, wherein the moisture content of red Ganoderma lucidum powder is 13.26%, with water as solvent, the solid-liquid ratio of red Ganoderma lucidum powder and water is 1: 25 (g / ml), first use microwave assisted Hot water extraction followed by ultrasonic-assisted hot water extraction of water-soluble Ganoderma lucidum polysaccharides. Amo...

Embodiment 2

[0042] (1) Use red ganoderma lucidum fruiting body as raw material to carry out superfine pulverization treatment to obtain red ganoderma powder with a particle size of about 200 mesh. The ratio of solid to liquid is 1:20 (g / ml), then centrifuged to remove the supernatant, and the resulting precipitate is dried in an oven at 60°C for 5 hours to obtain Ganoderma lucidum from which alcohol-soluble components, some monosaccharides, oligosaccharides and lipid substances have been removed pink.

[0043] (2) Accurately weigh 5g of red Ganoderma lucidum powder, wherein the moisture content of red Ganoderma lucidum powder is 13.26%, with water as solvent, the solid-liquid ratio of red Ganoderma lucidum powder and water is 1: 20 (g / ml), first adopt microwave-assisted Hot water extraction followed by ultrasound-assisted hot water extraction of water-soluble Ganoderma lucidum polysaccharides. Among them, the microwave extraction conditions are: microwave power 400W, extraction time 4min...

Embodiment 3

[0046] (1) Use red ganoderma lucidum fruiting body as raw material to carry out ultrafine pulverization treatment to obtain red ganoderma powder with a particle size of about 100 mesh, red ganoderma powder is refluxed with 95% ethanol at 75°C for 2 times, each time for 3 hours, the mixture of red ganoderma powder and ethanol The ratio of solid to liquid is 1:18 (g / ml), then centrifuged to remove the supernatant, and the resulting precipitate is dried in an oven at 60°C for 5 hours to obtain Ganoderma lucidum from which alcohol-soluble components, some monosaccharides, oligosaccharides and lipid substances have been removed pink.

[0047] (2) Accurately weigh 5g of red Ganoderma lucidum powder, wherein the moisture content of red Ganoderma lucidum powder is 13.26%, with water as solvent, the solid-liquid ratio of red Ganoderma lucidum powder and water is 1: 15 (g / ml), first adopt microwave-assisted Hot water extraction followed by ultrasound-assisted hot water extraction of wat...

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Abstract

The invention discloses a preparation method of ganoderma lucidum polysaccharide with high yield, which comprises the following steps: firstly, crushing ganoderma lucidum fruiting bodies into ganoderma lucidum powder, then sequentially extracting the ganoderma lucidum powder by adopting a microwave-assisted hot water extraction method and an ultrasonic-assisted hot water extraction method, and carrying out concentration, impurity removal, concentration, alcohol precipitation and drying on the obtained extracting solution to obtain water-soluble ganoderma lucidum polysaccharide; and extracting the ganoderma lucidum residues by adopting an alkali extraction method, and carrying out neutralization, dialysis, concentration, impurity removal, alcohol precipitation and drying on the obtained extracting solution to obtain alkali-soluble ganoderma lucidum polysaccharide. The method is used for extracting the ganoderma lucidum polysaccharide by combining microwave heating action and ultrasonic cavitation and extracting the alkali-soluble polysaccharide in the ganoderma lucidum by utilizing the alkali extraction method, thereby reducing the loss of the ganoderma lucidum polysaccharide; and the prepared ganoderma lucidum polysaccharide has the characteristics of high production yield, strong immunological activity and the like.

Description

technical field [0001] The invention provides a high-yield preparation method of ganoderma lucidum polysaccharide. Background technique [0002] Ganoderma lucidum is the general name of the fungi G. lucidum and G. japonicum belonging to the genus Ganoderma of the family Polyporaceae, also known as "Ganoderma lucidum", "Ganoderma lucidum" and "Emperor grass", First recorded in "Shen Nong's Herbal Classic", it has the effects of nourishing the middle and Qi, nourishing yin and strengthening, strengthening the body, prolonging life and so on. Ganoderma lucidum polysaccharide is one of the main functional components of Ganoderma lucidum. Pharmacological tests show that polysaccharides of Ganoderma lucidum have various pharmacological effects, such as anti-tumor, hypoglycemic, immune regulation, anti-oxidation, anti-virus and so on. Hot water extraction is a commonly used method to extract polysaccharides from Ganoderma lucidum. However, the traditional hot water extraction met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 黄生权敖宏宁正祥
Owner INFINITUS (CHINA) CO LTD
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