Hornet anti-bacterial peptide, its preparing method and use

An antibacterial peptide and wasp technology, applied in the field of biomedicine, can solve the problem of no hemolytic activity and cytotoxicity of antibacterial components

Inactive Publication Date: 2009-10-07
SUZHOU KANGTAI BIOLOGICAL PHARMACY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it has been found that the antibacterial components of wasp venom have no shortcomings such as hemolytic activity and cytotoxicity.

Method used

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  • Hornet anti-bacterial peptide, its preparing method and use
  • Hornet anti-bacterial peptide, its preparing method and use
  • Hornet anti-bacterial peptide, its preparing method and use

Examples

Experimental program
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Embodiment 1

[0051] The steps for preparing a Vespa antimicrobial peptide are as follows:

[0052] (1) Stimulate the wasp with a voltage of 6 volts, centrifuge the collected bee venom at a speed of 10,000 rpm for 10 minutes to remove the precipitate, and freeze-dry the supernatant to obtain a freeze-dried powder. Take 300 mg of lyophilized powder and dissolve in 5 ml of deionized water to obtain a sample solution.

[0053] (2) Gel filtration column chromatography: Sephadex G-50 filtration column (column length 1000 mm, diameter 26 mm) was used. Use eluent A (0.1 mol / L Na with a pH value of 6 2 HPO 4 -NaH 2 PO 4 buffer solution) to equilibrate the filter column, load the sample solution obtained in step (1) onto the filter column, then use eluent A to elute the filter column, and collect the solution of the third elution peak. (The results of gel filtration column chromatography are shown in the attached instructions. figure 1 , the third eluting peak part is shown in A. )

[0054] ...

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Abstract

The invention relates to a vespa antimicrobial peptide and its preparation method and application. The antimicrobial peptide is a single-chain polypeptide with a molecular weight of 1387.7 Daltons and an isoelectric point of 10. The sequence of the single-chain polypeptide is: isoleucine-tryptophan-tryptophan-lysine-glycine - Isoleucine - Alanine - Alanine - Methionine - Alanine - Lysine - Isoleucine. The preparation method is that the bee venom collected by electrically stimulating wasps is separated and purified through gel filtration column chromatography, ion exchange column chromatography and reversed-phase high-pressure liquid chromatography in sequence. The vespa antimicrobial peptide can be used to prepare medicines for treating diseases infected by pathogenic microorganisms and medicines for treating tumors.

Description

technical field [0001] The invention relates to a vespa antibacterial peptide and its preparation method and application, belonging to the field of biomedicine. Background technique [0002] Since the discovery of penicillin, people are no longer helpless against various diseases caused by pathogenic microorganism infection, and thus developed a large number of β-lactam antibiotics, which have made great contributions to human health. However, with the widespread use of the above-mentioned "traditional antibiotics", many new problems have arisen, such as long-term use leading to the emergence of drug-resistant strains, and even some pathogenic bacteria infections have no corresponding drugs for treatment. Therefore, the development of a new generation of antibacterial agents has become a top priority. Recent studies have found that polypeptide antibiotics not only have broad-spectrum antibacterial activity, but also do not induce the production of drug-resistant strains, wh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/00A61P35/00
Inventor 刘卫华李明
Owner SUZHOU KANGTAI BIOLOGICAL PHARMACY
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