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Cellulose-based degradable antibacterial peptide and preparation method thereof

A technology of cellulose and antimicrobial peptides, applied in the field of antimicrobial peptides, can solve the problems of poor resistance to proteolytic enzymes, reduced activity of antimicrobial peptides, and low stability

Active Publication Date: 2022-07-29
SHANGHAI MOYANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, a carboxymethylcellulose biomimetic antimicrobial peptide disclosed in the existing patent CN202011539085.2, although it does not affect the degradable characteristics of carboxymethylcellulose, it improves the modification efficiency based on carboxymethylcellulose , increased the antibacterial effect; but its antibacterial effect is still low, and because of its poor resistance to proteolytic enzymes, its stability in plasma is low, and the activity of antimicrobial peptides will be reduced in actual use

Method used

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  • Cellulose-based degradable antibacterial peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Step 1: Add 2 g of carboxymethyl cellulose to 20 mL of sulfuric acid solution with a concentration of 50%, acid treatment at room temperature for 2 hours, and ethanol to terminate the reaction; centrifuge at a centrifugal speed of 6000 rmp for 10 minutes, wash and freeze-dry to obtain fibers prime A;

[0028] Step 2: Disperse cellulose A in deionized water to obtain a cellulose solution; adjust pH=9, add 1-(3-dimethylaminopropyl)-3-ethylcarbeneimide, N-hydroxythiol Succinimide sodium salt, stirring for 2 hours; adding excess 5-diaminopentane, stirring for 12 hours, adding methoxypolyethylene glycol amine, reacting for a period of time; ethanol precipitation, centrifugation, washing, freeze-drying; It is ultrasonically dispersed in water, adjusted to pH=3.5 with 0.1M hydrochloric acid, stirred for 4 hours, washed, dialyzed and freeze-dried to obtain degradable antimicrobial peptides.

[0029] In the technical scheme, the added amount of 5-diaminopentane accounts for 20%...

Embodiment 2

[0031] Step 1: Add 2 g of carboxymethyl cellulose into 20 mL of sulfuric acid solution with a concentration of 50%, treat with acid for 1 to 6 hours at room temperature, and terminate the reaction with ethanol; centrifuge at a centrifugal speed of 6000 rmp for 10 minutes, wash, freeze-dry, to obtain cellulose A;

[0032]Step 2: Disperse cellulose A in deionized water to obtain 0.1 g / mL cellulose solution, adjust pH=9, add 1-(3-dimethylaminopropyl)-3-ethylcarbeneimide, N-Hydroxysulfosuccinimide sodium salt, stirred for 2 hours; added excess diamino compound, stirred for 8 hours; added methoxypolyethylene glycol amine, added 0.1M hydrochloric acid to adjust pH=5, stirred for 6 hours Ethanol precipitation, centrifugation, washing, freeze-drying; ultrasonically dispersing it in water, using 0.1M hydrochloric acid to adjust pH=3.5, stirring for 4 hours, adding tetrabutylammonium hydroxide solution dropwise, and continuing to stir for 3 hours; Washing, dialysis, freeze-drying to ob...

Embodiment 3

[0035] Step 1: Add 2 g of carboxymethyl cellulose into 20 mL of sulfuric acid solution with a concentration of 40%, acid treatment at room temperature for 6 hours, and ethanol to terminate the reaction; centrifuge at a centrifugal speed of 6000 rmp for 10 minutes, wash and freeze-dry to obtain fibers prime A;

[0036] Step 2: Disperse cellulose A in deionized water to obtain 0.1 g / mL cellulose solution, adjust pH=9, add 1-(3-dimethylaminopropyl)-3-ethylcarbeneimide, N-Hydroxythiosuccinimide sodium salt, stirred for 2 hours; added excess diamino compound, stirred for 6 hours; added methoxypolyethylene glycol amine, added 0.1M hydrochloric acid to adjust pH=5, stirred for 10 hours ; Ethanol precipitation, centrifugation, washing, freeze-drying; ultrasonically dispersing it in water, using 0.1M hydrochloric acid to adjust pH=3.5, stirring for 4 hours, adding tetrabutylammonium hydroxide solution dropwise, and continuing to stir for 4 hours; Washing, dialysis, freeze-drying to ob...

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Abstract

The invention discloses a degradable antibacterial peptide based on cellulose and a preparation method thereof. Comprising the following steps: step 1, adding cellulose into a sulfuric acid solution, carrying out acid treatment, and terminating the reaction with ethanol; centrifuging, washing and freeze-drying to obtain cellulose A; 2, dispersing cellulose A in deionized water to obtain a cellulose solution; the preparation method comprises the following steps: adjusting the pH value to 9, adding 1-(3-dimethylaminopropyl)-3-ethyl carbene imide and N-hydroxyl thiosuccinimide sodium salt, and stirring for 2 hours; adding excessive diamino compound, stirring for 10-12 hours, adding methoxy polyethylene glycol amine, and reacting for a period of time; carrying out ethanol precipitation, centrifugation, washing and freeze drying; the preparation method comprises the following steps: ultrasonically dispersing in water, adjusting the pH value to 3.5 by using 0.1 M hydrochloric acid, stirring for 2-4 hours, washing, dialyzing and freeze-drying to obtain the degradable antibacterial peptide.

Description

technical field [0001] The invention relates to the technical field of antimicrobial peptides, in particular to a cellulose-based degradable antimicrobial peptide and a preparation method thereof. Background technique [0002] Antibacterial peptide is a kind of basic polypeptide substance with antibacterial activity, drug resistance and environmental friendliness used to replace antibiotics. The antibacterial mechanism of antimicrobial peptides is related to the disruption of cell membranes by their amphiphilicity and positive charge. Due to the difficulty in extracting and purifying antimicrobial peptides from natural substances, and their high toxicity and low stability, their clinical application has been greatly restricted. However, by regulating the amphiphilicity in the polymer segment and regulating the molecular conformation through polymer simulation, synthetic antimicrobial peptides can be simulated and prepared. [0003] Natural cellulose is a wide-ranging and e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00A61K31/785A61K47/60A61K47/54A61P31/04
CPCC08G81/00A61K31/785A61K47/60A61K47/54A61P31/04Y02A50/30
Inventor 林光明江春阳秦照宇魏岱旭
Owner SHANGHAI MOYANG BIOTECHNOLOGY CO LTD
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