154 results about "Antibacterial protein" patented technology

The patent relates to a processing method for high quality vacuum packaging poultry product, which belongs to food processing and storing technology field. The invention takes fresh poultry chicken, duck or goose as material, which is made into poultry product which can be preserved under room temperature through slaying, segmentation, finishing, precooking, braising, vacuum packaging, sterilization and cooling etc. The invention adds anti-oxidative bioactive peptides into bittern-making process for effectively controlling flavor deterioration problem caused by lipid oxidation in sterilization process and preserving process, thus the vacuum packaging product can keep remarkable traditional flavor characteristics. The invention adds antibacterial peptide, antibacterial protein and spice extracts having natural antibacterial effect into bittern-making process, which decrease sterilization strength of sterilization process, and avoids flavor and texture deterioration caused by high temperature and pressure sterilization, thus the shelf life of product can last 6 months under room temperature.

The invention discloses a bacillus velezensis ZJ20 strain and liquid preparations thereof. The bacillus velezensis ZJ20 strain is preserved in China general microbiological culture collection center in Chaoyang district, Beijing, China under the number of CGMCC NO.5995 on April 13 2012. A microbial biocontrol agent, namely the novel bacillus velezensis ZJ20 strain, prepared by screening has endospores with high stress resistance and long shelf life, is highly environmentally adaptive and can be produced into commercial biological agents. Metabolites of the bacillus velezensis ZJ20 strain have high bactericidal power, have high colonization ability and fast speed on plants, and can occupy the surface of hybrid bamboo effectively to prevent dieback. Antibacterial protein (chitin with protein) produced by the strain is antibacterial, can promote growth of hybrid bamboo and various types of bamboo branches and leaves, and have a novel effect of recovering posterior production capacity.

The invention discloses a method for preparing antibacterial protein by utilizing aquatic product leftovers. The method comprises the following main steps: mincing the fresh aquatic product leftovers for later use, crushing vegetable feed proteins, and filtering the crushed vegetable feed proteins by virtue of a 60-mesh screen for later use; mixing the minced aquatic product leftovers and the crushed vegetable proteins, and uniformly mixing nutrients and water to be used as a fermenting culture base; sterilizing and cooling the fermenting culture base, placing bacillus subtilis HL-1 capable of generating subtilin, and carrying out the solid aerobic fermentation; drying, crushing and packaging the fermented product to obtain the antibactieral feed protein. The prepared antibacterial feed protein is rich in subtilin, nutritional small peptide and probiotics and has a good function of promoting the growth and resisting the diseases. By adopting the antibacterial feed protein, not only can the utilization rate of the protein be increased, but also the antimicrobial-free feed can be prepared by utilizing the antibacterial feed protein, and the application prospect and the social benefit are good.

The invention discloses an anti-bacterial protein PBR1 as well as a preparation method and application thereof, and relates to the technical field of molecular biology and biological pesticides. The amino acid sequence of the anti-bacterial protein PBR1 is shown as SEQ ID NO.1. The anti-bacterial protein PBR1 has a superior effect on preventing and curing plant diseases such as cruciferous plasmodiophoromycetes, paddy rice magnaporthe oryzae, curvularia lunata, fusarium oxysporum f. sp. dianthi, fusarium oxysporum vasinfectum, konjac foot rot bacteria, panax notoginseng root rot pathogen, pomegranate wilt pathogen, gaeumannomyces gramini, cylindrosporium pomi brooks, alternaria alternata and / or botrytis cinerea. A pesticide taking the anti-bacterial protein PBR1 as an active ingredient is a novel environment-friendly biological pesticide, is safe to people and livestock, protects the environment, and has a favorable development prospect.

The invention relates to bacillus amyloliquefaciens sp. Pb-4(T4,4), a preparation method of a conditioner, and an application. The bacillus amyloliquefaciens sp. is preserved in China General Microbiological Culture Collection Center (CGMCC), with the preservation number of CGMCC NO. 7155 and preservation date of January 18, 2013. The bacillus amyloliquefaciens sp. has obvious growth-promoting, antibacterial and disease-prevention effects on crops such as tomatoes, and can be colonized in soil and plants. The preparation method of the conditioner comprises the steps of liquid culture of bean product waste water and the like, and solid fermentation of soybean pod wall powder and the like, wherein a culture has viable bacteria as high as 4*10<9>cfu / ml and 5*10<9>cfu / g, and contains three growth-promoting antibacterial substances, namely antibacterial protein, enzyme and antibiotic. Waste recycling of the bean product waste water is realized, the cost is low, and the conditioner is suitable for industrial production, reduces or substitutes chemical pesticides, is safe and nontoxic, and meets the requirements of ecological agriculture and sustainable agricultural development.

The invention relates to Fenneropenaeus chinensis antibacterial protein and its coding gene, expression system, refolding system and application. The protein comprises amino acid sequences represented by SEQ ID No.2 in the sequence table or amino acid sequences represented by SEQ ID No.4 in the sequence table. The invention has the advantages that: a novel antibacterial protein Carcinin gene sequence is cloned from the Fenneropenaeus chinensis cDNA library, the Carcinin gene sequence encoding amino acid sequence is deduced; and the prokaryotic expressing system which is suitable for the recombinant expression in vitro of the antibacterial protein is screened out, thus the protein can be expressed efficiently, the recombinant protein can be refolded well, and the verification of biological functions of the recombinant protein shows that the recombinant protein has growth inhibitory effects on Grampositive bacteria and has no growth inhibitory effects on Gram negative bacteria. The clone and recombinant expression of the gene has an important application prospect in developing novel antibacterial drugs.

The invention relates to a Fenneropenaeus chinensis antimicrobial protein gene and the recombination expression and the application. It has the amino acid sequence of SEQ ID No.2 and basic group sequence of SEQ ID No.1 in sequence table. It selects the carrier of the antimicrobial protein pCR T7 / NT TOPO TA and host bacterium BL21(DE3)pLysS that could high efficiently express recombination protein. Aimed at the feature of plural disulfide bonds, using the oxidation reduction system made of oxidized form of glutathione and glutathione in separating and purification and protein recombination process, the recombination protein with antimicrobial ability would be gained. The protein has strong inhibition to the growth of gram positivebacteria, and some inhibition to gram negativebacteria.

The invention relates to an antifungal protein separated and purified from bacillus subtilis J18, a protein product, a preparation method and an application. A strain serial number of the bacillus subtilis J18 is CGMCC No.3665, and a strain name is the bacillus substilis J18. The preparation method of the protein product comprises the following steps of: mixing the J18 antifungal protein with 1 percent of acetic acid to form a chitosan solution with a pH value of 6.0, compositing the chitosan solution to form a film coating reagent which has the concentration of 0.05mg / mL and is used for soaking a fruit, and also coating a mixture which is formed by mixing the J18 antifungal protein solution with an oxidized starch solution on a preservative paper to prepare an antifungal preservative paper used for wrapping the fruit. The antifungal protein has remarkable control effect to fruit and crop diseases and particularly black spot diseases of pears after picking and is an antagonistic strain with broad spectrum and high efficiency.

The invention discloses a method for representing activity relationships of antibacterial proteins or polypeptides. The method is characterized in that starting from 85 structural parameters and topological indexes of representation molecules, a characteristic descriptor comprising 166 amino acid systems is obtained through principle component analysis (PCA), namely a principle component score vector of structural and topological variable (VSTPV).. The VSTPV is applied to study of the quantitative structure-activity relationships (QSAR) of 101 antibacterial cationic peptides and 28 hydrolyzed bovine lactoferricins (BPPs), the models have better fitting and forecasting capabilities, and the cross validation related coefficients and related coefficients are above 0.70. The study shows that the VSTPV can well represent coded and uncoded amino acids and establish polypeptides with stronger forecasting capabilities and derivative QSAR models thereof.

The present invention relates to a kind of bacillus preparation for preventing and controlling pest and disease of crop. The effective components of the bacillus preparation includes spores of Bacillus subtilis and bacillus thringiensis. Spores has high resistance and stable effect in field, and the two kinds of bacillus are different in functional mode and have no mutual interference. The bacillus thringiensis after being eaten by lepidopterous pests will cause lethal septicaemia; while Bacillus subtilis is a kind of beneficial bacillus capable of spreading widely in soil and on the surface of plant body and secreting antibacterial protein with powerful antagonistic effect to harmful bacteria to resist the invasion.

The present invention relates to a novel bacteriophage-originated protein having antimicrobial activity, more precisely an antimicrobial protein originated from lytic bacteriophage having killing activity specific to Staphylococcus aureus which is the causing agent of infectious diseases in human and animals, a pharmaceutical composition for the prevention and treatment of the disease caused by Staphylococcus aureus, an antibiotic and a disinfectant containing the bacteriophage-originated antimicrobial protein as an active ingredient.

The invention discloses a coral-associated marine bacillus amyloliquefaciens strain CoMb-9 and an application thereof. The strain is preserved in the Guangdong Microbiological Culture Collection Center on Floor 5, Building 59, Courtyard 100, Xianlie Middle Road, Guangzhou, Guangdong Province on April 19, 2016, and the preservation number is GDMCC No. 60028. The strain has strong effects of inhibiting growth of plant phytophthora bacteria, plant wilt pathogenic bacteria and / or plant colletotrichum orbiculare and the like, can be colonized to propagate in soybeans, hot peppers, bananas and other crops or in rhizosphere soil of the above crops, secretes to produce antifungal proteins, indoleacetic acid and other active materials, has the effects of promoting crop growth and preventing and treating multiple plant diseases, can be applied to developing and preparing bio-organic fertilizers, biological disease prevention preparations and other agricultural biological agents with effects of promoting crop growth, improving crop yield and preventing and treating the multiple plant diseases, and has excellent popularization and application prospects in related agricultural production.

The invention discloses a controlled-release antibacterial edible composite film and a preparation method thereof. Soy isolate protein serves as a main substrate, glycerin, pectin, nisin, sodium lactate and the like are added according to a specific proportion, so that antibacterial activity and the mechanical performance of the film are improved, and coating is performed for three times by the aid of ultrasound-assisted technology to prepare a controlled-release edible antibacterial protein composite film. The antibacterial activity of the composite film can be effectively improved by nisin-sodium lactate bacteriostatic agents, and microbe development is prevented. Release of the bacteriostatic agents can be effectively controlled by a multilayer film structure prepared by controlled-release technology, and the antibacterial activity of the film is greatly enhanced. The antibacterial activity of the antibacterial composite film slightly changes within two months during storage period, the mechanical performance also slightly changes, the diameter of an antibacterial ring is decreased by 10-15%, tensile strength is reduced by 3-9%, water permeability is enhanced by 2-7%, oxygen permeability is enhanced by 4-5.5%, the color of the film is transparent and does not remarkably change, and the effective period of food quality is prolonged. The composite film is simple to operate, stable and reliable in product quality and suitable for industrial production.

The invention discloses a preparation method of DWD (Double Whey Acidic Protein Domain) antibacterial peptide molecules of fenneropenaeus chinensis, and discloses an application of DWD antibacterial peptide in the aspect of preparing antibacterial proteins and protease inhibitor proteins. The recombined DWD antibacterial peptide of the fenneropenaeus chinensis, which is prepared by utilizing the method disclosed by the invention, can be used for directly inhibiting the growth of common bacteria and inhibiting the activity of proteases secreted in the growth process of the bacteria and has a greater application prospect on the aspects of food antibacterial biological reagents and medical antibiotic substitutes.

The invention discloses a method for purifying antibacterial protein in bacillus cereus fermentation broth. The method comprises the following steps of: culturing the bacillus cereus fermentation broth through microbial fermentation by utilizing autonomously screened and identified Bacillus cereus (CGMCC (China General Microbiological Culture Collection Center) NO.4348), extracting an antibacterial protein crude product by utilizing a ammonium sulfate fractional precipitation method, further purifying the antibacterial protein crude product by utilizing a sephadex column and detecting the bacteriostatic activity on fungal diseases. The method disclosed by the invention has the advantages of convenience for operation, simple process, low cost and capability of remarkably increasing the antibacterial activity of the antibacterial protein in the fermentation broth.

The present invention relates to one kind of extracellular antiseptic protein of Bacillus subtilis and its separation and purification process. The extracellular antiseptic protein has molecular weight of 41.9 kD, 26 mass-to-charge ratio peaks, and 5 peptide amino acid sequences including VTIVDEKGR, FSFSDVHNR, VYIADSTNFK, ELPISENLASVNMR and EAEWAYMITGK. Its separation and purification process includes the following steps: separating coarse protein from Bacillus subtilis Bs-916 via DEAE Sepharose F.F. separation, separating its B peak drips via Phenyl Sepharose 6 F.F. separation, separating the E peak drips via with hydroxyapatite column and obtaining the G peak drips as the purified extracellular antiseptic protein. The extracellular antiseptic protein has broad spectrum antiseptic activity, ribonuclease activity and agglutinin activity.

The invention belongs to the field of biochemistry, and discloses an antibacterial protein LYS-ABP and an antibacterial protein MBP-LYS-ABP having an LYS-ABP protein N terminal added with MBP, and preparation methods thereof, wherein the antibacterial protein LYS-ABP and the antibacterial protein MBP-LYS-ABP have no stimulation to human bodies and have good antibacterial effect. The antibacterial protein LYS-ABP and the antibacterial protein MBP-LYS-ABP have no stimulation to the human bodies and have good antibacterial effect, the antibacterial effect is better than single muramidases and antibacterial peptide proteins, and the biological activity stability is good. The antibacterial protein MBP-LYS-ABP is obtained by in-vitro recombinant expression, and is easy to prepare and relatively low in cost; and the antibacterial protein MBP-LYS-ABP has high soluble expression yield and is suitable for large-scale preparation. The antibacterial protein LYS-ABP is obtained by enzyme digestion of a recombinant expression-prepared MBP-LYS-ABP fusion protein with thrombin, and is easy to prepare and relatively low in cost.

The invention relates to a PopW antibacterial protein and biological bactericide mixed biological preparation PopW-PF1, belonging to the technical field of plant protection. The total concentration of viable bacteria in a biological bactericide prepared from pseudomonas fluorescens is 1*10<9>CFU / ml; PopW antibacterial protein has the concentration of 30mg / ml and is diluted into 300-30000 times for use; and in the mixed biological preparation PopW-PF1, the PopW antibacterial protein and the biological bactericide have mixed volume ratio of (30000-30):1, and diluted into 50-100 times when in use. The mixed biological preparation PopW-PF1 has control effect reaching above 50 percent on viral diseases on vegetable crops of pepper, tomato and the like and grain crops of rice, bean and the like, and better control effect on other diseases on the ground such as leaf mold and ustilaginoidea virens, wherein the increase production effect reaches 10 percent.

The present invention relates to one kind of extracellular antiseptic protein of Bacillus subtilis and its separation and purification process. The extracellular antiseptic protein has molecular weight of 41.9 kD, 26 mass-to-charge ratio peaks, and 5 peptide amino acid sequences including VTIVDEKGR, FSFSDVHNR, VYIADSTNFK, ELPISENLASVNMR and EAEWAYMITGK. Its separation and purification process includes the following steps: separating coarse protein from Bacillus subtilis Bs-916 via DEAE Sepharose F.F. separation, separating its B peak drips via Phenyl Sepharose 6 F.F. separation, separating the E peak drips via with hydroxyapatite column and obtaining the G peak drips as the purified extracellular antiseptic protein. The extracellular antiseptic protein has broad spectrum antiseptic activity, ribonuclease activity and agglutinin activity.

The invention discloses a method for separating antibacterial proteins from bacillus pumilus E14. The method comprises the following steps: preparing a zymophyte solution through fermentation production, and centrifuging to prepare supernatant; precipitating by using ammonium sulfate, resuspending the obtained precipitate by using a buffer solution to obtain resuspension; separating through an ion exchange method, collecting an elution peak, concentrating, dialyzing, and obtaining an active ingredient separation peak; and measuring the molecular weight of the antibacterial proteins separated from the bacillus pumilus E14 to be between 97.2 and 116.0kD through antibacterial activity detection and protein electrophoresis detection. The method is simple and reasonable in design and convenient to operate, and proteins with an effect of resisting Vibrio harveyi ((i) Vibrio( / i) (i) harveyi( / i) can be separated according to the method.

The invention discloses a microbial preparation, and specifically discloses a microbial preparation prepared from pig-derived bacillus licheniformis YHSH-02, and an application of the microbial preparation to aquaculture. The strain disclosed by the invention is preserved in the China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms at No. 3, No. 1 yard, Beichen West Road, Chaoyang district, Beijing, the preservation number is CGMCC NO.15454, and the preservation date is 15th March, 2018. The bacillus licheniformis YHSH-02 disclosedby the invention is separated from intestines of wild boars, can produce rich small peptide, lipopeptide type bacillin, antibacterial protein and the like, can restrain growth of various aquaculture pathogenic bacteria of vibrio parahaemolyticus, edwardsiella tarda, aeromonas hydrophila, pseudoalteromonas nigrifaciens, vibrio vulnificus and the like, and can be used for preventing and treating aquaculture diseases caused by various bacteria of the vibrio parahaemolyticus, the edwardsiella tarda, the aeromonas hydrophila, the pseudoalteromonas nigrifaciens, the vibrio vulnificus and the like. The situation that the microbial preparation can effectively prevent and treat black-foot diseases of prawns is found for the first time, an effective scheme is provided for preventing and treating theblack-foot diseases of the prawns, and the microbial preparation has important significance. The bacillus licheniformis YHSH-02 obtained from the intestines of the wild boars is a bacterial strain which is high in preventing and treating efficiency, wide in preventing and treating range and notable in growth promotion and has biological preventing and treating potential, and has favorable application and development prospects.

The invention relates to the field of water-treatment materials and discloses an antibacterial composite reverse osmosis membrane for high-efficiency water treatment and a preparation method. The preparation method comprises the following preparation processes of: (1) immersing a porous polysulfone supporting membrane into piperazine aqueous solution, n-hexane solution of trimesoyl chloride and glycerinum aqueous solution containing formaldehyde successively, and after taking out, preparing out a polyamide desalting layer on the surface of the porous polysulfone supporting film; (2) preparingdispersion liquid by adopting the following components in parts by weight: 25-30 parts of bacillus-natto antibacterial protein, 22-25 parts of protamine, 5-8 parts of sodium lactate and 37-48 parts ofwater; (3) after mixing the dispersion liquid and glutamine transaminase, coating on single surface of the polyamide desalting layer, drying and preparing the antibacterial composite reverse osmosismembrane. Compared with the traditional antibacterial composite reverse osmosis membrane, the antibacterial composite reverse osmosis membrane disclosed by the invention is firm in antibacterial layerand difficult in dropping, can generate bacteriostatic action for multiple strains, and is good in bacteriostatic effect, stable in bacteriostatic property and long in service life.

The invention provides barley worm natural antibacterial protein and a preparation method and application thereof. According to the barley worm natural antibacterial protein and the preparation method and application thereof disclosed by the invention, escherichia coli and / or staphylococcus aureus are / is used as an inducer, after bacterial liquid is injected to barley worm larvae or yellow mealworm larvae, then the barley worm larvae or the yellow mealworm larvae are raised for some time, hemolymph of the larvae is collected, and antibacterial protein is extracted. The obtained antibacterial protein can be used for preparing an antibacterial agent or a preservative. The antibacterial protein provided by the invention has the characteristics of being good in broad-spectrum antibacterial properties, high in stability, good in safety and the like, the preparation technology is simple, raw materials are cheap and easy to obtain, and the application prospect is great.

The invention relates to the field of molecular biology, and aims at providing a Cotesia vestalis Teratocyte Defensin CvT-Def gene and an encoded antibacterial polypeptide. A nucleotide sequence of the gene is as shown in SEQ ID NO. 1, and an amino acid sequence of the antibacterial polypeptide is as shown in SEQ ID NO. 2. The Cotesia vestalis Teratocyte Defensin CvT-Def gene and an amino acid sequence encoded thereby are of a novel hymenopteran-derived antibacterial protein, can be developed into food additives and medicaments having application value and are applied to multiple fields of agriculture, food sanitation, industry and the like.

The invention discloses a clone, an expression and an application of an antimicrobial protein HAS1 encoding gene. The nucleotide sequence of the antimicrobial protein HAS1 encoding gene is shown in SEQIDNO.1, and the amino acid sequence is shown in SEQIDNO.2. The sequences are obtained by taking a biocontrol strain HAS as a research object, performing the antibacterial mechanism, taking genome DNA (deoxyribonucleic acid) of the strain HAS as a template, using a specific expression primer to perform PCR (polymerase chain reaction) amplification to obtain the full expression sequences of the encoding gene, then, constructing a prokaryotic expression vector to express the sequences, and at last taking a sugar cane smut virus as a target to carry out an expression product bacteriostasis experiment. The experiment results show that the expression product has the same capability of resisting against the sugar cane smut virus. The online comparison shows that the antimicrobial protein is a novel one; and encoding the gene sequences of the antimicrobial protein can provide a new material for the future breeding for disease resistance.

The invention discloses a fermentation method for improving the yield of bacillus subtilis antibacterial protein. The method comprises the following steps: firstly obtaining microbial strain, successively inoculating the obtained microbial strain to a seed culture medium and a fermentation culture medium to perform fermentation, so as to obtain the bacillus subtilis antibacterial protein, and performing stage adjustment according to growth conditions of thallus in a logarithmic growth phase within 6 to 16 hours after the beginning of fermentation of the thallus and in a stable phase from 16 hours after the beginning of fermentation of the thallus to the end of the fermentation and according to the fermentation time. The method has the advantages that the inoculation amount of the microbialstrain is regulated before the fermentation of the bacillus subtilis BNCC 189983 antibacterial protein, the temperature, dissolved oxygen, pH value and shaking table rotate speed of a system during fermentation are regulated, the microbial strain can rapidly and stably grow in the early stage of fermentation through the regulation of the parameters, the microbial strain can efficiently and stablyproduce antibacterial protein in the middle and later stage, and the production of other side products is reduced.

The invention provides a plant expression vector for a vascular peculiar promoter to control two types of antimicrobial protein gene and a method for cultivating greensickness-resistant cotton. In the method, motherwort antimicrobial protein genes LjAMP2 and LjAMP2 controlled by a vascular tissue peculiar promoter AAP2 are integrated into a cotton genome to realize the high-amount expression of the antimicrobial protein gene in the vascular tissue of the transgenic cotton root and properly express in the vascular tissues of other parts of the plant so as to obviously improve the resistance to greensickness by cotton. After performing homozygosis to defoliating and nondefoliating greensickness bacteria inoculated by a T2 generation plant greenhouse disease pool, the transgenic cotton obtained with the method compares the defoliating and nondefoliating greensickness bacteria with Non-GMO, and disease index can be respectively lowered by 40% and 80%.

The invention relates to the technical field of microbial control, in particular to a bacillus siamensis YW17 strain, a biocontrol inoculant and application of the bacillus siamensis YW17 strain in soil-borne disease control and / or plant growth promotion. The preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) The YW17 strain provided by the invention can generate antibacterial protein, has an inhibition effect on fusarium oxysporum causing soil-borne diseases, has the bacteriostasis rate of 49.7%, and has the advantages of environmental protection, high efficiency and safety. Furthermore, the YW17 strain provided by the invention can secrete heteroauxin to promote plant growth.

A nucleic acid sequence encoding a polypeptide having antibacterial activity, which nucleic acid sequence has the nucleotide sequence of Bases 198 to 452 of Seq. ID No. 13 or is a nucleic acid that hybridizes under highly stringent conditions to a complement of a nucleic acid having the nucleotide sequence of Bases 198 to 452 of Seq. ID No. 13, and the polypeptide encoded thereby.