Growth-promoting antibacterial bacillus amyloliquefaciens sp., preparation method of conditioner and application
A technology of amylolytic spores and conditioners, which is applied in the application field of microorganisms in agriculture, and can solve the problems that restrict the sustainable development of facility production, frequent occurrence of soil-borne diseases, and imbalance of soil nutrients, and achieve high antibacterial and disease prevention effects. Wide antibacterial spectrum and low cost effect
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[0038] (3) Preparation of solid conditioner Use various cheap and easy-to-obtain agricultural and sideline products as fermentation material: fermentation material components: bean curd powder: 30-33g, bran 25-30g, chaff 5-10g, bean cake powder 5-30g 10g, 12-20g corn flour, 20-30% water content; adjust the pH to be neutral; sterilize by autoclaving at 121°C for 1h. When it is cooled to about 45°C, add 3-10% seed liquid into the solid fermentation material. Static culture at 30~35℃ for 50~72h, the number of viable bacteria can be obtained ≥4*10 9 cfu / g. dry basal culture. It is a solid conditioner.
[0039] The characteristic of the production of the conditioner is that it uses the leftovers of agricultural and sideline products such as bean pods, bean stalks, and rice husks discarded during soybean harvesting. It is also cheap and environmentally friendly.
[0040] Three, Bacillus amyloliquefaciens PB-4 (T4,4) antibacterial spectrum in the present embodiment
[0041] The a...
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