34 results about "Fast protein liquid chromatography" patented technology

An ultrahigh-pressure dual on-line solid phase extraction / capillary reverse-phase liquid chromatography (DO-SPE / cRPLC) system is provided. The system comprises: a sample loading valve into which a first solvent and a sample to be analyzed are loaded; a first column valve in flow communication with a first solid-phase extraction column and a first reverse-phase liquid chromatography column; a second column valve in flow communication with a second solid-phase extraction column and a second reverse-phase liquid chromatography column; a column-switching valve for determining whether the sample is transferred to either the first column valve or the second column valve; a solvent selection valve in flow communication with the first and second column valves to supply the first solvent or a mixed solvent of the first solvent and a second solvent to the first and second column valves; a second solvent loading valve, in flow communication with a solvent mixer, into which the first and second solvents are loaded; a supply pump for loading the second solvent into the second solvent loading valve; and a supply pump for loading the first solvent into the sample loading valve, the second solvent loading valve and the solvent selection valve. The system requires minimal time (i.e. dead time) for column equilibration between successive experiments to shorten the total time required for the experiments by a factor of about two. In addition, the system enables rapid sample injection, on-line sample desalting and sample enrichment. Furthermore, the system is highly reproducible in terms of liquid chromatography (LC) retention time and can be operated at a pressure as high as 10,000 psi.

The invention discloses a preparation method for quickly separating plant proanthocyanidin dimer and trimer. The method comprises the following steps: performing liquid-liquid extraction on an alcohol solution rich in plant proanthocyanidin raw material, and then flowing by a gel column, performing FPLC (Fast Protein Liquid Chromatography) or HSCCC (High Speed Counter Current Chromatography) separation, thereby separating and acquiring the proanthocyanidin dimer and trimer components. The technology disclosed by the invention is suitable for the separation and purification of the proanthocyanidin dimer and trimer from different resources and has universality. The proanthocyanidin dimer and trimer acquired according to the preparation method disclosed by the invention are higher in purity. The method has the advantages of high safety, simple operation, less time consumption, low production cost, environmental protection, reaching green chemical standard, suitability for industrial production and ultrahigh commercial value.

The invention relates to a matrine liquid chromatographic determination method in the chemical detection technical field. The method comprises the following steps: respectively diluting a matrime sample and a matrime reference substance with methanol to volume, taking aqueous solution containing the methanol, phosphoric acid and triethylamine as a mobile phase and injecting into a liquid chromatograph, and then sequentially determining precision, 24h stability, reproducibility and recovery rate of the matrine sample. The determination method has the advantages of simple operational steps, strong adaptability and good reproducibility of analysis results.

The invention relates to a method for determining synthesized intermediate and product in wastewater in TATB production by liquid chromatogram, belonging to the technical field of chemical analysis. The method comprises the following steps: firstly, preparing standard solutions of phloroglucinol, TETNB and TATB standard substances by using liquid phase chromatogram mobile phase; secondly, treating the TATB wastewater, regulating pH to be neutral, extracting to-be-detected substance, drying the to-be-detected substance, adding the liquid phase chromatogram mobile phase for dissolving, filtering through a microfiltration membrane to remove impurity to obtain a liquid phase chromatogram detection sample; thirdly, performing liquid phase chromatographic analysis on the standard solutions, and drawing a standard curve; and fourthly, respectively analyzing the samples by using the liquid phase chromatogram, comparing the analysis result with the standard curve to determine the intermediate and the product in the TATB wastewater. Trace amount of intermediate related substances, such as phloroglucinol, TETNB and the product, namely TATB in the TATB wastewater can be accurately detected by using the method disclosed by the invention, and the defects in the prior art are filled.

The invention discloses a separation and purification method of c-di-GMP (cyclic diguanylate), comprising the following steps of: directly sampling a c-di-GMP crude product solution on a fast protein liquid chromatography equipped with an ion exchange chromatographic column; carrying out gradient elution by using a buffer solution with pH of 8.0; carrying out detection under 254 nm by an ultraviolet detector; collecting an eluent in the peak position; confirming a structure by using a mass spectrum; and freeze-drying the eluent with target compounds, thereby obtaining the solid c-di-GMP. The c-di-GMP prepared according to the method provided by the invention has the purity of over 95% through reversed-phase high performance liquid chromatography detection, and realizes c-di-GMP separation and purification from the c-di-GMP crude product solution in one step; moreover, the method provided by the invention has simple technology, short period and nonuse of toxic organic solvents, and is a novel method with high efficiency, environment friendliness and suitability for industrialized production.

Methods of making functionalized support material are disclosed. Functionalized support material suitable for use in chromatography columns or cartridges, such as in a high pressure liquid chromatography (HPLC) column or a fast protein liquid chromatography (FPLC) column, is also disclosed. Chromatography columns or cartridges containing the functionalized support material, and methods of using functionalized support material, such as a media (e.g., chromatographic material) in a chromatography column or cartridge, are also disclosed.