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A kind of plant proanthocyanidin dimer, trimer rapid separation preparation method

A proanthocyanidin and trimer technology, applied in the direction of organic chemistry, can solve the problems of complex components of proanthocyanidin from black wattle bark, no separation and purification method, complicated process, etc., and achieve high commercial value and short time consumption , high purity and high yield

Active Publication Date: 2018-07-20
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In 2011, Rie Kusano et al obtained pure proanthocyanidins from black wattle extract, but the process was complicated and the yield was low
The composition of proanthocyanidins from black wattle bark is more complex, and no simple and industrial separation and purification method has been found

Method used

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  • A kind of plant proanthocyanidin dimer, trimer rapid separation preparation method
  • A kind of plant proanthocyanidin dimer, trimer rapid separation preparation method
  • A kind of plant proanthocyanidin dimer, trimer rapid separation preparation method

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Experimental program
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Effect test

Embodiment 1

[0028] Add 10g of crushed and sieved black wattle bark raw material to 100mL of 60% ethanol according to the ratio of solid to liquid 1:10, and extract with ultrasonic wave at 50°C for 30min, and obtain the extract after filtration. The extract was concentrated by rotary evaporation, and the extract was dissolved in 200 mL of water. Add 100mL petroleum ether to the aqueous solution and extract (three times) to obtain the aqueous layer. The aqueous layer was extracted with 600 mL of ethyl acetate (three times) to obtain an ethyl acetate layer. The ethyl acetate layer is spin-dried and freeze-dried to obtain the black wattle bark ethyl acetate extract, and the liquid chromatography analysis results of its components are shown in figure 1 . Depend on figure 1 It can be seen that the components of the black wattle bark extract are complex after being extracted with ethyl acetate, and the overlapping peaks in the liquid phase diagram indicate that there are isomers of proanthocy...

Embodiment 2

[0034] Add 10g of crushed and sieved grape seed raw material to 100mL of 60% ethanol according to the ratio of solid to liquid 1:10, and extract with ultrasonic wave at 50°C for 30min, and obtain the extract after filtration. The extract was concentrated by rotary evaporation, and the extract was dissolved in 200 mL of water, and the aqueous solution was extracted with 100 mL of petroleum ether (three times) to obtain an aqueous layer. The aqueous layer was extracted with 600 mL of ethyl acetate (three times) to obtain an ethyl acetate layer. The ethyl acetate layer was spin-dried and freeze-dried to obtain the grape seed ethyl acetate extract, and the liquid chromatography analysis results of its components were shown in Figure 6 . Depend on Figure 6 It can be seen that the crude grape seed extract is extracted with ethyl acetate to obtain grape seed oligomers, which have complex components and need further separation and purification.

[0035] Dissolve 2 g of grape seed...

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Abstract

The invention discloses a preparation method for quickly separating plant proanthocyanidin dimer and trimer. The method comprises the following steps: performing liquid-liquid extraction on an alcohol solution rich in plant proanthocyanidin raw material, and then flowing by a gel column, performing FPLC (Fast Protein Liquid Chromatography) or HSCCC (High Speed Counter Current Chromatography) separation, thereby separating and acquiring the proanthocyanidin dimer and trimer components. The technology disclosed by the invention is suitable for the separation and purification of the proanthocyanidin dimer and trimer from different resources and has universality. The proanthocyanidin dimer and trimer acquired according to the preparation method disclosed by the invention are higher in purity. The method has the advantages of high safety, simple operation, less time consumption, low production cost, environmental protection, reaching green chemical standard, suitability for industrial production and ultrahigh commercial value.

Description

technical field [0001] The invention belongs to the technical field of rapid separation and preparation methods for flavan-3-ol dimers and trimers, and in particular relates to a rapid separation and preparation method for plant proanthocyanidin dimers and trimers. Background technique [0002] Proanthocyanidins are a general term for a large class of polyphenolic compounds that exist widely in nature, and are polyphenolic compounds of a class of flavanol monomers and their polymers. Polyphenols have strong antioxidant capacity and other biological activities, which are beneficial to human health. Among them, proanthocyanidins are the most abundant class of plant polyphenols, and they have attracted increasing attention. [0003] Proanthocyanidins have a higher V E , Vc, higher bioavailability of carotene, and can be combined with V E produce synergistic oxidation. In recent years, proanthocyanidins are mainly used in medicine, health care products, cosmetics and function...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 王飞周蒙梅雪婷姜萍李迅黄申林张瑜
Owner NANJING FORESTRY UNIV
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