Matrine liquid chromatography measuring method

Abstract

The invention relates to a matrine liquid chromatographic determination method in the chemical detection technical field. The method comprises the following steps: respectively diluting a matrime sample and a matrime reference substance with methanol to volume, taking aqueous solution containing the methanol, phosphoric acid and triethylamine as a mobile phase and injecting into a liquid chromatograph, and then sequentially determining precision, 24h stability, reproducibility and recovery rate of the matrine sample. The determination method has the advantages of simple operational steps, strong adaptability and good reproducibility of analysis results.

Description

technical field [0001] The invention relates to a method in the technical field of chemical detection, in particular to a liquid chromatography determination method for matrine. Background technique [0002] High-performance liquid chromatography analysis method is a commonly used method in drug analysis. This method is based on classical liquid chromatography and introduces the theory and technology of gas chromatography. Realize online separation and analysis. It has the characteristics of wide application range, high separation efficiency, fast analysis speed and high sensitivity. Therefore, in recent years, it has been widely used in the separation, identification and content determination of the chemical components of Chinese herbal medicines and their preparations. [0003] Matrine is one of the main alkaloids contained in leguminous plants Sophora flavescens, Sophora flavescens and Shandou root. It is mainly used clinically to treat cancer, viral hepatitis, heart di...

AI Technical Summary

Problems solved by technology

Among them, most HPLC methods use ion-pairing reagents or strong acid-base, or use amino columns and ion-exchange columns, and the elution methods are mostly gradient elution. The shortcomings of these analytical methods are: ① ion-pairing reagents or strong The introduction of acid and alkali is easy to produce precipitation, which will cause system leakage due to friction between the plunger and the sealing pad of the high-pressure pump in the high-performance liquid chromatograph; ②Amino column and ion exchange column are expensive, short in life, and difficult to maintain;③ The solvent ratio of the gradient elution method changes with time, and each separation needs to be rebalanced. The analysis time is long, the operation is complicated, and the reproducibility is not as good as that of isocratic elution; ④The mobile phase preparation is quite complicated and difficult to operate

However, this technology has the disadvantages of complex operation, poor component separation and poor reproducibility of analysis results.

[0005] After searching, it was found that Hou Huichan published "Determination of Oxymatrine and Matrine in Shandou Root" in "Chinese New Medicine" (2004, Vol.3-No.9-P.83), which records A method for simultaneously determining the content of matrine and oxymatrine in the root of the mountain bean root by RP-HPLC method, but this technology has the disadvantages of complex mobile phase preparation and narrow linear range

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