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87 results about "Cell sorter" patented technology
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Microfabricated Cell Sorter
InactiveUS20080176211A1Efficient separationIncrease chanceImmobilised enzymesSludge treatmentInlet channelCell sorter
Owner:CALIFORNIA INST OF TECH
Multi-way sorter system and method
ActiveUS8753573B2Avoid pollutionBioreactor/fermenter combinationsBiological substance pretreatmentsCell sorterEngineering
Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.
Owner:BECTON DICKINSON & CO
Microfabricated cell sorter
InactiveUS7214298B2High sorting rateEfficient separationSludge treatmentVolume/mass flow measurementInlet channelCell sorter
The invention provides a microfabricated device for sorting cells based on a desired characteristic, for example, reporter-labeled cells can be sorted by the presence or level of reporter on the cells. The device includes a chip having a substrate into which is microfabricated at least one analysis unit. Each analysis unit includes a main channel, having a sample inlet channel, typically at one end, and a detection region along a portion of its length. Adjacent and downstream from the detection region, the main channel has a discrimination region or branch point leading to at least two branch channels. The analysis unit may further include additional inlet channels, detection points, branch points, and branch channels as desired. A stream containing cells is passed through the detection region, such that on average one cell occupies the detection region at a given time. The cells can be sorted into an appropriate branch channel based on the presence or amount of a detectable signal such as an optical signal, with or without stimulation, such as exposure to light in order to promote fluorescence.
Owner:CALIFORNIA INST OF TECH
Cell sorter chip having gel electrodes
A cell sorting chip and a cell sorting technology are to be established which can positively detect and sort a specified cell for cell separation and detection using micro flow paths formed on a substrate, whereby a cell analyzing / sorting device is provided which uses an inexpensive disposable chip replaceable for each sample. To this end, micro flow paths formed on the substrate are formed, and cells are roughly sorted in a first stage and then finely sorted in a second stage. More specifically, cells are sorted roughly by using scattered light or according to intensity of luminescence in the first stage. In the second stage, the roughly sorted cells are sorted with high precision using image recognition.
Owner:HITACHI LTD +2
Cell sorter micro-fluidic chip based on immunomagnetic separation technology and application thereof in aspect of enrichment of rare cells
InactiveCN101643701AReduce manufacturing costEase of mass productionMicrobiological testing/measurementBiomass after-treatmentAntigenCell sorter
The invention discloses a cell sorter micro-fluidic chip based on the immunomagnetic separation technology and application thereof in the aspect of enrichment of rare cells, belonging to the technicalfield of micro-fluidic chips. The micro-fluidic chip, improved on the basis of the existing Y-type micro-fluidic chip, comprises a substrate and a cover-slip, wherein a Y-shaped micro-channel, a sample cell (1) and a buffer cell (2) respectively located at two end points of the upper part of the Y-shaped micro-channel, and an effluent cell (3) located at the end point of the lower part of the Y-shaped micro-channel are arranged on the substrate; and an antigen / antibody immunological reaction chamber (4) located on the micro-channel between the cross point (5) of the Y-shaped micro-channel andthe effluent cell (3) is further arranged on the substrate, wherein the width of the antigen / antibody immunological reaction chamber (4) is 2 to 5 times that of the micro-channel. The chip of the invention can effectively and rapidly fix the magnetic beads, increase the sample size of cells, improve the analysis flux, reduce the dead volume of sample introduction and reduce the retention and cross infection.
Owner:TSINGHUA UNIV
Cell sorter system and method
ActiveUS9200334B2Increased complexityImprove precisionBioreactor/fermenter combinationsBiological substance pretreatmentsCell sorterTime delays
Provided herein are improved cell sorter systems and methods. Such systems and methods provide a self-stabilizing sorter jet to automate calibration, and address the issue of drift in cell sorting systems. The systems and methods presented make it possible to determine and set the charge delay interval automatically with circuitry in the cell sorter. These circuits can set, monitor, and adjust the time delay continuously, allowing for a completely automatic, autonomous, turn-key, self-stabilizing sorter jet.
Owner:BECTON DICKINSON & CO
Cell sorter and culture system
InactiveUS20070238169A1Bioreactor/fermenter combinationsBiological substance pretreatmentsElectricityCell sorter
Methods and apparatus are provided for culturing cells under conditions for determining cellular differentiation and for separating cells from culture media based on differentiation. The apparatus comprises a bioreactor, media reservoir, a magnetic cell separator, an inlet port for adding magnetic particles to the bioreactor, and a circulating pump, wherein the bioreactor, media reservoir, magnetic cell separator and inlet port are on a single fluid circuit. The present method and apparatus provides a method for separating cells from culture without removing the cells from culture, so that un-selected cells may be returned to the bioreactor for further culture. The method employs magnetic labeling in culture, where the magnetic label specifically identifies cells to be distinguished, either by separation or retention in the culture. The method and apparatus are further designed to comprise means for electromechanical stimulation of hum embryonic stem cells for preparation of electrically responsive tissue.
Owner:THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIV
Rapid Single Cell Based Parallel Biological Cell Sorter
ActiveUS20120142018A1Small sizeImprove design flexibilityBioreactor/fermenter combinationsBiological substance pretreatmentsBiological cellCell sorter
A disposable rapid cell sorter comprises of microfluidic chip with electrodes and sorts biological cells of interest through magnetic field and electric field based on biological cell functional antibody bonded magnetic beads and luminescent labeling.
Owner:CYTEK BIOSCI
Imaging cell sorter
InactiveUS20150132766A1Accurate analysisImage enhancementBioreactor/fermenter combinationsCell sorterFluorescence
The present invention provides a cell enrichment / purification device having a function of continuously enriching cells, a function of locating the cells in a particular area of a flow path in a continuous array after the cell enrichment, a function of recognizing the shape of the cells and fluorescence emission from the cells at the same time in units of one cell based on an image, and a function of recognizing the cells based on the information on the shape and fluorescence emission to separate / purify the cells.
Owner:ON CHIP CELLOMICS CONSORTIUM +1
Method and apparatus for cell sorting
InactiveUS7160730B2Bioreactor/fermenter combinationsBiological substance pretreatmentsCell sorterFluorescence
Owner:UNIV OF MARYLAND +1
Detachable cell-delivery system for patch-clamp unit
ActiveUS7056430B1Improve throughputLow costImmobilised enzymesElectrostatic separatorsCell sorterEngineering
The cell-delivery unit of a high-throughput electrophysiological testing system is implemented as a reusable movable unit suitable for repetitive delivery of cells to a disposable, multi-aperture patch-clamp tray. An electric field emanating from the patch aperture is used to align the dispenser with the aperture. A set of electrodes in the nozzle of the dispenser is used to detect the electric field and effect the alignment. According to another aspect of the invention, dielectrophoretic fields produced by sets of electrodes in the nozzle form a retaining cage that is used first to suspend a test cell directly above the patch aperture and then to urge the cell toward it. A movable cell sorter may also be coupled to the cell-delivery unit.
Owner:MOLECULAR DEVICES
Inducing culture method for regulatory T cell
ActiveCN102168067AGood energyShort cycleBlood/immune system cellsIndividual particle analysisCell sorterRegulatory T cell
The invention provides an inducing culture method for regulatory T cells and relates to an inducing culture method for regulatory gamma delta T cells. In the method, cell factors are applied and deoxyribonucleic acid (DNA) demethylated medicament decitabine is combined so as to cooperatively induce the generation of the regulatory gamma delta T cells, and an enrichment process of magnetic cell sorter (MACS) positive sorting is adopted; after enrichment, detections of cell growth state and activity are carried out so as to ensure the next experience. The method has the advantages of simple and practicable inducing and enrichment processes, short period, low cost, high inducing efficiency and good repeatability; and after enrichment, cell activity is good, the quantity and activity of cells can ensure the next in vivo and in vitro studies, thereby providing a good study platform for defining the biological characteristics of the gamma delta T cells. Thus, the method has a good popularization value.
Owner:ZHEJIANG UNIV
Microfluidic cell sorter utilizing broadband coherent Anti-stokes raman scattering
InactiveUS20110207207A1Enhanced output signalBioreactor/fermenter combinationsBiological substance pretreatmentsCell sorterIlluminance
A microfluidic cell sorter has a microfluidic structure (500) with a sample input channel (1 1 0) leading into an observation region (214), two buffer channels (1 1 8) configured to hydrodynamically focus a sample target cell (208) within the observation region, and at least two output channels (1 1 4). Apparatus directs the target cell into a selected output channel based on a cell sorting control signal (61 6). A CARS pulse source (524) generates CARS pulses (526), which are directed to the target cell within the observation region. A detector (530) detects CARS illumination scattered from the target signal and generates a spectrum signal based on the detected illumination. A processor (61 4) identifies the target cell based on the spectrum signal and generates the cell sorting control signal based on the identity of the target cell.
Owner:UNIV OF COLORADO THE REGENTS OF
Human papilloma virus (HPV) detection using nucleic acid probes, microbeads and fluorescent-activated cell sorter (FACS)
Owner:GENERA BIOSYSTEMS LTD
Methods for Isolating Monocytes
InactiveUS20080248011A1Increase opportunitiesBiocideNervous disorderCell sorterCell Membrane Proteins
The present invention provides HIDE1 as novel monocyte markers. Since HIDE1 are membrane proteins, monocytes can be specifically detected by using antibodies that bind to HIDE1. Further, HIDE1-positive monocytes can also be collected from peripheral blood or the like using a cell sorter, magnet, or such. Monocytes that can be prepared based on the present invention are useful in cell immunotherapy.
Owner:MEDICAL & BIOLOGICAL LAB CO LTD
Continuous whole-chip 3-dimensional dep cell sorter and related fabrication method
A 3-dimensional PDMS cell sorter having multiple passages in a PDMS layer that follow the same path in a DEP separation region and that are in fluid communication with each other within that region. The passages may differ in width transverse to the flow direction within the passages. Flat plates may sandwich the PDMS layer; each plate may have a planar electrode used to generate a DEP field within a sample fluid flowed within the passages. The DEP field may concentrate target cells or particulates within one of the passages within the DEP separation region. The passages may diverge after the DEP-separation region, leaving one passage with a high concentration of target cells or particulates. Techniques for manufacturing such structures, as well as other micro-fluidic structures, are also provided.
Owner:RGT UNIV OF CALIFORNIA
Microfluidic cell sorter and method
InactiveUS20110189714A1Bioreactor/fermenter combinationsBiological substance pretreatmentsCell sorterSub populations
A biological particle sorting device and method of its use. The device includes structure arranged to urge biological particles into substantially single file travel through an interrogation zone. Operable alignment structure nonexclusively include sheathed fluid flow, capillary tubes, an orifice, and fluid microchannels. One or more detector, selected from a plurality of operable such structures, may be employed to sense the presence of a biologic particle in the interrogation zone. Certain exemplary detectors may operate on the Coulter principle, or may detect a Stokes' shift, or side-scatter radiation. Discrimination structure is generally provided to categorize particles as being in one or another sub-population of a mix of biological particles that may be carried in a fluid sample, such as by cell type, size, or the like. Killing structure, such as a laser, is disposed to neutralize all particles in any undesired sub-population while leaving undamaged the desired sub-population(s). The device may be operated to essentially purify (in a living or viable sense) a sample including biological particles that are carried in a fluid diluent.
Owner:ORFLO TECH LLC
Multi-Way Sorter System and Method
ActiveUS20120276621A1Avoid pollutionBioreactor/fermenter combinationsOpening closed containersCell sorterEngineering
Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern.
Owner:BECTON DICKINSON & CO
System and methods for anaerobic environmental microbial compartmentalized cultivation
InactiveUS9556413B2High throughput isolationBioreactor/fermenter combinationsBiological substance pretreatmentsCell sorterBiology
The invention described below relates to an enclosed cell sorting device and methods of using the device. The device is constructed so that the entire process of cell sorting can be conducted under fully anaerobic conditions to retain viability of anaerobic cells before, during, and after cell sorting. This is accomplished by creating an anaerobic atmosphere for the high speed cell sorter and all its components and by the use of airlocks that allow the introduction of anaerobic containers into the chamber containing the sample.
Owner:SYNTHETIC GENOMICS INC
Cell sorter and cell sorting method
ActiveCN102564920AReliable sortingDielectrophoresisIndividual particle analysisDielectricThermodynamics
The invention relates to a cell sorter and a cell sorting method. The cell sorter includes a measuring electrode, a working electrode, a detection electrode, and an output section. The measuring electrode forms a measuring electric field in a flow path to measure a complex dielectric constant of each cells flowing through the flow path. The working electrode forms, in the flow path, a working electric field to sort the cells by imparting a dielectrophoretic force to the cells and using the flow path. The detection electrode detects the presence of the cell in the fluid flowing through the flow path. The output section acquires a sorting signal based on information about the measured complex dielectric constant and a detection signal indicating the detection of the cell by the detection electrode. The output section outputs a working signal adapted to form the working electric field to the working electrode when the detection signal is acquired if the sorting signal is acquired.
Owner:SONY CORP
Method for controlling release velocity of fluorescent probe molecule in emulsions
InactiveCN101231233ASmall toxicityOrganic active ingredientsMaterial analysis by optical meansCell sorterFluorescence
A method for controlling the speed of releasing the fluorescent probe molecules in the latex emulsion belongs to the material controlled release technical field. The art is as follows: a fluorescent probe molecule water solution or a polymer-fluorescent probe molecule water solution taken as the water phase is added into a mineral oil phase containing a polylol non-ionic surface promoting agent, the volume ratio of the polylol non-ionic surface promoting agent to the mineral oil ranges from 1:19 to 1:10, the solution is evenly stirred at a rotating speed of 8000 to 12000 turns per minute under 273 to 298 K for 1 to 5 minutes to get a water-in-oil latex emulsion, a 0.8 milliliters of water solution containing two to four percent of polyoxyethylene sorbitan laurate is added into to the prepared water-in-oil latex emulsion to be emulsionized for the second time for 2 to 5 minutes to obtain the water-in-oil or oil-in-water latex double emulsion. The sample is analyzed by a fluorescent energetic cell sorter and the release speed of the fluorescent probe molecules is calculated by a formula. The invention has the advantages of high speed, high efficiency, directness and accuracy.
Owner:UNIV OF SCI & TECH BEIJING
Method for preparing foot-and-mouth disease virus-resistant RNAi transgenic livestock
InactiveCN101979607AReduce infection rateHas the ability to resist FMDAnimal reproductionClimate change adaptationBase JInfection rate
The invention discloses a method for preparing foot-and-mouth disease virus-resistant (FMDV) transgenic livestock by expressing FMDV-resistant ribonucleic acid interference (RNAi), which comprises the following steps of: linearizing an FMDV-resistant RNAi recombinant Lenti-virus vector which carries a short hairpin RNA (shRNA) base sequence and a green fluorescent protein (GFP) reporter gene and transfecting fetus fibroblasts of normal livestock; sieving transgenic nuclear donor cells by using the GFP reporter gene and a flow cell sorter and introducing nuclear donor nuclei into denucleated oocytes of the livestock to obtain a reconstructed embryo; and immigrating the reconstructed embryo into the uterus of surrogate livestock to obtain an offspring, namely the FMDV-resistant RNAi heterozygote transgenic livestock. Heterozygote transgenic male livestock and heterozygote transgenic female livestock are normally mated or reproduced by artificial insemination so as to obtain homozygote transgenic livestock. The transgenic livestock prepared by the method can express the FMDV-resistant RNAi, obviously reduces the FMDV infection rate and has the capability of resisting foot-and-mouth disease (FMD).
Owner:DAIRY CATTLE RES CENT SHANDONG ACADEMY OF AGRI SCI
Efficient PD-1-CD8+T cell culture method
InactiveCN106591232ASpecific damageHigh cytotoxic activityCell dissociation methodsCulture processCell sorterMicrobiology
The invention discloses an efficient PD-1-CD8+T cell culture method, which specifically comprises: extracting TIL cells; screening the TIL cell suspension by using a flow cell sorter to obtain purified PD-1-CD8+T cells; adding the purified PD-1-CD8+T cells to a G-Rex gas permeable bottle, and culturing for 1 week with a complete culture medium; and rapidly amplifying the PD-1-CD8+T cells. The efficient PD-1-CD8+T cell culture method of the present invention has advantages of effective microbial contamination rate reducing, high amplification efficiency, high cytotoxic activity, and the like.
Owner:安徽安龙基因科技有限公司
Method and apparatus for cell sorting
InactiveUS20040077033A1Bioreactor/fermenter combinationsBiological substance pretreatmentsCell sorterFluorescence
A cell sorter system that includes a precision pump that causes fluid containing cells to enter an inlet port where the fluid is exposed to source light of one or more wavelengths and where cells scatter light or produce fluorescence by known means, and the scattered light and / or fluorescence is used to detect the presence and position of desired cells. The fluid is passed into a select gate that can be magnetostrictive where it is cause to exit one of a plurality of ports, at least one of these ports receiving the desired cells. A controller controls the precision pump, the detection system, and the select gate so that cells are selected from the fluid. An optional vacuum system can be used to pull fluid or cells out of the selected exit port.
Owner:UNIV OF MARYLAND +1
Isolation and Culture-Expansion Methods of Mesenchymal Stem/Progenitor Cells From Umbilical Cord Blood, And Differentiation Method of Umbilical Cord Blood-Derived Meschymal Stem/Progenitor Cells Into Various Mesenchymal Tissues
InactiveUS20100184218A1Increase the number ofMicrobiological testing/measurementCulture processAntigenProgenitor
There is provided a method for the isolation and cultivation of mesenchymal stem / progenitor cells from umbilical cord blood, and also to a method for the differentiation of the umbilical cord blood-derived mesenchymal stem / progenitor cells into various mesenchymal tissues. The method include overlaying umbilical cord blood onto Ficoll-Hypaque solution; centrifuging the umbilical cord blood on the Ficoll-Hypaque solution to obtain a mononuclear cell layer; reacting cells obtained by monolayer culture of the mononuclear cells with antibodies to mesenchymal stem / progenitor cell-specific antigens for a predetermined period of incubation time; isolating only cells bound to their corresponding antibodies using a cell sorter; and cultivating the isolated cells, thereby obtaining mesenchymal stem / progenitor cells with high purity and excellent viability.
Owner:MEDIPOST
Human papilloma virus (HPV) detection using nucleic acid probes, microbeads and fluorescent-activated cell sorter (FACS)
Owner:INCITE HEALTH PTY LTD
Cell sorter
InactiveCN109504598AImproved dispensing accuracyBioreactor/fermenter combinationsDielectrophoresisCell sorterEngineering
An embodiment relates to a cell sorter that separates fine particles such as specific cells from a sample liquid. The cell sorter separates specific particles in the sample liquid without damage on particles such as cells in the sample liquid. The cell sorter according to the embodiment includes a flow channel (20) which supplies a sample liquid containing particles from one end side; a pluralityof branch channels (21, 22) which are connected to the other end side of the flow channel (20); an image sensor (40) which has a pixel region covering the flow channel (20) and the branch channels (21, 22); a determination unit (50) which determines the characteristics of the particles in the sample liquid from a measurement signal of the pixel region of the portion of the flow channel (20) of theimage sensor (40); and a separation unit (60) which is provided adjacently to the connection of the flow channel (20) and the branch channels (21, 22) and guides the particles in the sample liquid toany of the branch channels (21, 22) based on the determination result of the determination unit (50).
Owner:KK TOSHIBA
Light irradiating apparatus for cell sorter
A light irradiating apparatus for a cell sorter for irradiating light to a specimen in a sample liquid includes a light source for emitting light at a plurality of wavelengths, a light extracting member for separately extracting a plurality of monochromatic light from the emitted light, said respective monochromatic light having respective peak wavelengths, a directing member for directing said monochromatic light extracted by the light extracting member, and an irradiating member for simultaneously irradiating said plurality of monochromatic light to a position where the sample liquid passes through.
Owner:AISIN SEIKI KK
High throughput screening for novel bioactivities
InactiveUS20050040550A1Microbiological testing/measurementLibrary screeningCo encapsulationExpression Library
Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nuclei acid directly isolated from the environment; and (ii) screening said libraries utilizing a fluorescence activated cell sorter to identify said clones. More particularly, this is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; (ii) exposing said libraries to a particular substrate or substrates of interest; and (iii) screening said exposed libraries utilizing a fluorescence activated cell sorter to identify clones which react with the substrate or substrates. Also provided is a process for identifying clones having a specified activity of interest by (i) generating one or more expression libraries derived from nucleic acid directly or indirectly isolated from the environment; and (ii) screening said exposed libraries utilizing an assay requiring co-encapsulation, a binding event or the covalent modification of a target, and a fluorescence activated cell sorter to identify positive clones.
Owner:DIVERSA CORP
Modular monitor and control system for cell sorter stream
InactiveUS20150146962A1Continuous monitoringPrevent or minimize any potential contamination of the sorted cellsComputer controlFlow control using electric meansDigital videoCell sorter
A modular monitor and control system which monitors the fluid jet and droplet formation region in a stream-in-air cell or particle sorter and controls sheath fluid and sample flows via the first and second valves. The system includes a processor which receives digital video image signals of the fluid jet and droplet formation region in the sorter; continuously analyzes the digital image signals to determine a significant change in the digital image signal; and triggers at least a relay switch activation signal in response to a determined significant change. At least one relay switch which is separate from the cell sorter and in communication with the processor is connected with the first and second valves and configured to close the first and second valves upon activation of the relay switch via the relay switch activation signal.
Owner:RUTGERS THE STATE UNIV
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