Cyclomycin family antibiotic enzyme-linked immunoassay reagent kit

A technology of enzyme-linked immunoassay and detection kit, which is applied in the field of enzyme immunoassay, and can solve the problems of lack of sensitivity and specificity and high pretreatment requirements of microbial inhibition method

Inactive Publication Date: 2006-08-02
镇江出入境检验检疫局检验检疫综合技术中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbial inhibition and immunoassay are suitable for routine screening methods, physical and chemical methods are used for identification and quantification, but microbial inhibition lacks sensitivity and specificity, physical and chemical methods require specialized staff, high requirements for sample pretreatment, and immunoassay The method is specific and sensitive, and it is one of the methods with the most development potential

Method used

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  • Cyclomycin family antibiotic enzyme-linked immunoassay reagent kit
  • Cyclomycin family antibiotic enzyme-linked immunoassay reagent kit
  • Cyclomycin family antibiotic enzyme-linked immunoassay reagent kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1 Preparation of coated antigen

[0038] Weigh 1 g of tetracycline hydrochloride and dissolve it in 50 mL of water, add 2.7 g of chlorosuccinimide, react for 30 minutes, filter, and wash the crude product with water. The crude product was treated with 150 mL of water and diethyl ether (volume ratio 1:1), the water phase was discarded, the diethyl ether phase was evaporated to dryness, washed with water, and filtered to obtain the pure product (about 5.6 g), which was modified tetracycline.

[0039]Dissolve 20mg of modified tetracycline and 6mg of succinic anhydride in 2mL of dioxane, react at 37°C for 2 hours, and place at 4°C overnight. This is solution ①. Take another 35mg of ovalbumin (OVA) dissolved in 2mL of water, and then add 1mL of dioxane, this is the ② solution. Then take 125 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and dissolve it in 2 mL of water. h, slowly drop the reaction solution into ② solution, stir for 4 hours, then a...

Embodiment 2

[0055] Antigen: use oxytetracycline hydrochloride standard substance instead, antibody: use oxytetracycline antibody solution instead. The ELISA plate was coated with oxytetracycline-coated antigen, and the configuration of the remaining reagent bottles was the same as in Example 1, which was the oxytetracycline ELISA kit.

Embodiment 3

[0057] Antigen: use aureomycin hydrochloride standard substance instead, antibody: use aureomycin antibody solution instead. The ELISA plate was coated with aureomycin-coated antigen, and the configuration of the rest of the reagent bottles was the same as in Example 1, which was the aureomycin enzyme-linked immunoassay kit.

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Abstract

The present invention relates to a tetracycline group antibiotic ELIA kit, belonging to the field of enzymoimmunoanlysis technology. Said invention adopts the enzymoimmunoadsorption, protein coupling and biochemical preparation technique to prepare the invented detection kit. Its key technique lies in that after the tetracycline group is renovated, it is coupled with protein BSA by using succinyl oxide as bridge to prepare coating antigen, the tetracgcline group is coupled with protein BSA by using glutaraldehyde as bridge to synthesize immunoantigen, then the enzymoimmunoadsorption indirect competition method can be used to detect tetracycline group. Its detection range is 1950ng/g-1.90ng/g; its detection time only requires 4 hr.

Description

technical field [0001] An enzyme-linked immunoassay kit for tetracycline family antibiotics belongs to the technical field of enzyme immunoassay. Applied to the detection of tetracycline antibiotics in animal source foods, especially meat products, aquatic products, bee products and dairy products. Background technique [0002] Tetracycline antibiotics include chlortetracycline, oxytetracycline and tetracycline, etc., which can be used as veterinary drugs for the prevention and treatment of livestock and poultry diseases, or as feed additives to promote the growth of livestock and poultry, and are used in the aquaculture industry to treat various fish diseases; In the dairy industry, it is used to increase the milk production of cows; in the beekeeping industry, it is used to prevent infectious diseases of bees. However, excessive use will inevitably cause antibiotics such as mothers and metabolites to remain in the muscles, eggs, milk, and organs of food-borne animals, whi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/543G01N33/535
Inventor 潘荣生宓晓黎李平李利东徐文久陆茂林朱金连周洪斌袁建兴成恒嵩
Owner 镇江出入境检验检疫局检验检疫综合技术中心
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